Influence of preservation techniques and freezing storage time on biochemical composition and spectrum of fatty acids of Isochrysis galbana clone T-ISO

Isochrysis galbana clone T‐ISO was cultured in 5‐L vessels with continuous light at 100 µmol photons m−2 s−1 at temperature room (22 °C) and harvested at early exponential phase. Dry weight (dw), ash content, biochemical composition and fatty acid profile were determined in fresh biomass and after storage for 7 days and 3 months at −76 °C for both freeze‐dried and frozen biomasses. Both freezing and freeze‐drying caused significant losses in dry weight (range 13.67–19.74%) when compared with fresh microalgae (40.17 pg cell−1), irrespective of storage time. Ash content increased significantly (P