Combined effects of proinflammatory cytokines and intermittent cyclic mechanical strain in inhibiting osteogenicity in human periodontal ligament cells
Mechanical strain plays an important role in bone formation and resorption during orthodontic tooth movement. The mechanism has not been fully studied, and the process becomes complex with increased amounts of periodontal patients seeking orthodontic care. Our aims were to elucidate the combined eff...
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| Veröffentlicht in: | Cell biology international 2016-09, Vol.40 (9), p.999-1007 |
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| creator | Sun, Chaofan Chen, Lijiao Shi, Xinlian Cao, Zhensheng Hu, Bibo Yu, Wenbin Ren, Manman Hu, Rongdang Deng, Hui |
| description | Mechanical strain plays an important role in bone formation and resorption during orthodontic tooth movement. The mechanism has not been fully studied, and the process becomes complex with increased amounts of periodontal patients seeking orthodontic care. Our aims were to elucidate the combined effects of proinflammatory cytokines and intermittent cyclic strain (ICS) on the osteogenic capacity of human periodontal ligament cells. Cultured human periodontal ligament cells were exposed to proinflammatory cytokines (interleukin‐1β 5 ng/mL and tumor necrosis factor‐α 10 ng/mL) for 1 and 5 days, and ICS (0.5 Hz, 12% elongation) was applied for 4 h per day. The autocrine of inflammatory cytokines was measured by enzyme‐linked immunosorbent assay. The expression of osteoblast markers runt‐related transcription factor 2 and rabbit collagen type I was determined using real‐time polymerase chain reaction and Western blot. The osteogenic capacity was also detected by alkaline phosphatase (ALP) staining, ALP activity, and alizarin red staining. We demonstrated that ICS impaired the osteogenic capacity of human periodontal ligament cells when incubated with proinflammatory cytokines, as evidenced by the low expression of ALP staining, low ALP activity, reduced alizarin red staining, and reduced osteoblast markers. These data, for the first time, suggest that ICS has a negative effect on the inductive inhibition of osteogenicity in human PDL cells mediated by proinflammatory cytokines. |
| doi_str_mv | 10.1002/cbin.10641 |
| format | Article |
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The mechanism has not been fully studied, and the process becomes complex with increased amounts of periodontal patients seeking orthodontic care. Our aims were to elucidate the combined effects of proinflammatory cytokines and intermittent cyclic strain (ICS) on the osteogenic capacity of human periodontal ligament cells. Cultured human periodontal ligament cells were exposed to proinflammatory cytokines (interleukin‐1β 5 ng/mL and tumor necrosis factor‐α 10 ng/mL) for 1 and 5 days, and ICS (0.5 Hz, 12% elongation) was applied for 4 h per day. The autocrine of inflammatory cytokines was measured by enzyme‐linked immunosorbent assay. The expression of osteoblast markers runt‐related transcription factor 2 and rabbit collagen type I was determined using real‐time polymerase chain reaction and Western blot. The osteogenic capacity was also detected by alkaline phosphatase (ALP) staining, ALP activity, and alizarin red staining. We demonstrated that ICS impaired the osteogenic capacity of human periodontal ligament cells when incubated with proinflammatory cytokines, as evidenced by the low expression of ALP staining, low ALP activity, reduced alizarin red staining, and reduced osteoblast markers. These data, for the first time, suggest that ICS has a negative effect on the inductive inhibition of osteogenicity in human PDL cells mediated by proinflammatory cytokines.</description><identifier>ISSN: 1065-6995</identifier><identifier>EISSN: 1095-8355</identifier><identifier>DOI: 10.1002/cbin.10641</identifier><identifier>PMID: 27357508</identifier><language>eng</language><publisher>England: Blackwell Publishing Ltd</publisher><subject>Adolescent ; Adult ; Cell Differentiation - physiology ; Cells, Cultured ; Cytokines ; Cytokines - immunology ; Cytokines - metabolism ; Female ; human periodontal ligament cells ; Humans ; ICS ; IL-1β ; Inflammation - metabolism ; Ligaments ; Male ; Orthodontics ; Osteoblasts - cytology ; Osteoblasts - metabolism ; Osteogenesis - physiology ; osteogenicity ; Periodontal Ligament - cytology ; Periodontal Ligament - immunology ; Periodontal Ligament - metabolism ; Periodontitis - metabolism ; Periodontitis - pathology ; proinflammatory cytokines ; Stress, Mechanical ; TNF-α ; Tooth Mobility - immunology ; Tooth Mobility - metabolism ; Young Adult</subject><ispartof>Cell biology international, 2016-09, Vol.40 (9), p.999-1007</ispartof><rights>2016 International Federation for Cell Biology</rights><rights>2016 International Federation for Cell Biology.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3951-83b9fa8ca18a8ef17ea344792b9e36f1e20bbce067fcd5fb07019f74630e8c773</citedby><cites>FETCH-LOGICAL-c3951-83b9fa8ca18a8ef17ea344792b9e36f1e20bbce067fcd5fb07019f74630e8c773</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fcbin.10641$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fcbin.10641$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27357508$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sun, Chaofan</creatorcontrib><creatorcontrib>Chen, Lijiao</creatorcontrib><creatorcontrib>Shi, Xinlian</creatorcontrib><creatorcontrib>Cao, Zhensheng</creatorcontrib><creatorcontrib>Hu, Bibo</creatorcontrib><creatorcontrib>Yu, Wenbin</creatorcontrib><creatorcontrib>Ren, Manman</creatorcontrib><creatorcontrib>Hu, Rongdang</creatorcontrib><creatorcontrib>Deng, Hui</creatorcontrib><title>Combined effects of proinflammatory cytokines and intermittent cyclic mechanical strain in inhibiting osteogenicity in human periodontal ligament cells</title><title>Cell biology international</title><addtitle>Cell Biol Int</addtitle><description>Mechanical strain plays an important role in bone formation and resorption during orthodontic tooth movement. The mechanism has not been fully studied, and the process becomes complex with increased amounts of periodontal patients seeking orthodontic care. Our aims were to elucidate the combined effects of proinflammatory cytokines and intermittent cyclic strain (ICS) on the osteogenic capacity of human periodontal ligament cells. Cultured human periodontal ligament cells were exposed to proinflammatory cytokines (interleukin‐1β 5 ng/mL and tumor necrosis factor‐α 10 ng/mL) for 1 and 5 days, and ICS (0.5 Hz, 12% elongation) was applied for 4 h per day. The autocrine of inflammatory cytokines was measured by enzyme‐linked immunosorbent assay. The expression of osteoblast markers runt‐related transcription factor 2 and rabbit collagen type I was determined using real‐time polymerase chain reaction and Western blot. The osteogenic capacity was also detected by alkaline phosphatase (ALP) staining, ALP activity, and alizarin red staining. We demonstrated that ICS impaired the osteogenic capacity of human periodontal ligament cells when incubated with proinflammatory cytokines, as evidenced by the low expression of ALP staining, low ALP activity, reduced alizarin red staining, and reduced osteoblast markers. These data, for the first time, suggest that ICS has a negative effect on the inductive inhibition of osteogenicity in human PDL cells mediated by proinflammatory cytokines.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Cell Differentiation - physiology</subject><subject>Cells, Cultured</subject><subject>Cytokines</subject><subject>Cytokines - immunology</subject><subject>Cytokines - metabolism</subject><subject>Female</subject><subject>human periodontal ligament cells</subject><subject>Humans</subject><subject>ICS</subject><subject>IL-1β</subject><subject>Inflammation - metabolism</subject><subject>Ligaments</subject><subject>Male</subject><subject>Orthodontics</subject><subject>Osteoblasts - cytology</subject><subject>Osteoblasts - metabolism</subject><subject>Osteogenesis - physiology</subject><subject>osteogenicity</subject><subject>Periodontal Ligament - cytology</subject><subject>Periodontal Ligament - immunology</subject><subject>Periodontal Ligament - metabolism</subject><subject>Periodontitis - metabolism</subject><subject>Periodontitis - pathology</subject><subject>proinflammatory cytokines</subject><subject>Stress, Mechanical</subject><subject>TNF-α</subject><subject>Tooth Mobility - immunology</subject><subject>Tooth Mobility - metabolism</subject><subject>Young Adult</subject><issn>1065-6995</issn><issn>1095-8355</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc9u1DAQxiMEoqVw4QGQJS6oUsCO4zg-wopuK5Xlwh-Ji-U44123ib3YjiBPwuvi7LY9cECy5JHm932ama8oXhL8lmBcvdOddblqavKoOCVYsLKljD1e6oaVjRDspHgW4w3GhNRt87Q4qThlnOH2tPiz8mOWQ4_AGNApIm_QPnjrzKDGUSUfZqTn5G8zFJFyPbIuQRhtSuBSbunBajSC3ilntRpQTEFZhw5vZzubrNsiHxP4LWTCpnnp7aZRObSHYH3vXcq6wW7VeLCEYYjPiydGDRFe3P1nxdeLj19Wl-X15_XV6v11qalgJC_aCaNarUirWjCEg6J1zUXVCaCNIVDhrtOAG250z0yHOSbC8LqhGFrNOT0r3hx9884_J4hJjjYuEygHfoqStIRUQnAmMvr6H_TGT8Hl6RYKN5RXvMnU-ZHSwccYwMh9sKMKsyRYLnHJJS55iCvDr-4sp26E_gG9zycD5Aj8sgPM_7GSqw9Xm3vT8qix-ei_HzQq3MqGU87k981a_vi0qcU3spaX9C-Yt7LQ</recordid><startdate>201609</startdate><enddate>201609</enddate><creator>Sun, Chaofan</creator><creator>Chen, Lijiao</creator><creator>Shi, Xinlian</creator><creator>Cao, Zhensheng</creator><creator>Hu, Bibo</creator><creator>Yu, Wenbin</creator><creator>Ren, Manman</creator><creator>Hu, Rongdang</creator><creator>Deng, Hui</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201609</creationdate><title>Combined effects of proinflammatory cytokines and intermittent cyclic mechanical strain in inhibiting osteogenicity in human periodontal ligament cells</title><author>Sun, Chaofan ; Chen, Lijiao ; Shi, Xinlian ; Cao, Zhensheng ; Hu, Bibo ; Yu, Wenbin ; Ren, Manman ; Hu, Rongdang ; Deng, Hui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3951-83b9fa8ca18a8ef17ea344792b9e36f1e20bbce067fcd5fb07019f74630e8c773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Cell Differentiation - physiology</topic><topic>Cells, Cultured</topic><topic>Cytokines</topic><topic>Cytokines - immunology</topic><topic>Cytokines - metabolism</topic><topic>Female</topic><topic>human periodontal ligament cells</topic><topic>Humans</topic><topic>ICS</topic><topic>IL-1β</topic><topic>Inflammation - metabolism</topic><topic>Ligaments</topic><topic>Male</topic><topic>Orthodontics</topic><topic>Osteoblasts - cytology</topic><topic>Osteoblasts - metabolism</topic><topic>Osteogenesis - physiology</topic><topic>osteogenicity</topic><topic>Periodontal Ligament - cytology</topic><topic>Periodontal Ligament - immunology</topic><topic>Periodontal Ligament - metabolism</topic><topic>Periodontitis - metabolism</topic><topic>Periodontitis - pathology</topic><topic>proinflammatory cytokines</topic><topic>Stress, Mechanical</topic><topic>TNF-α</topic><topic>Tooth Mobility - immunology</topic><topic>Tooth Mobility - metabolism</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sun, Chaofan</creatorcontrib><creatorcontrib>Chen, Lijiao</creatorcontrib><creatorcontrib>Shi, Xinlian</creatorcontrib><creatorcontrib>Cao, Zhensheng</creatorcontrib><creatorcontrib>Hu, Bibo</creatorcontrib><creatorcontrib>Yu, Wenbin</creatorcontrib><creatorcontrib>Ren, Manman</creatorcontrib><creatorcontrib>Hu, Rongdang</creatorcontrib><creatorcontrib>Deng, Hui</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cell biology international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sun, Chaofan</au><au>Chen, Lijiao</au><au>Shi, Xinlian</au><au>Cao, Zhensheng</au><au>Hu, Bibo</au><au>Yu, Wenbin</au><au>Ren, Manman</au><au>Hu, Rongdang</au><au>Deng, Hui</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Combined effects of proinflammatory cytokines and intermittent cyclic mechanical strain in inhibiting osteogenicity in human periodontal ligament cells</atitle><jtitle>Cell biology international</jtitle><addtitle>Cell Biol Int</addtitle><date>2016-09</date><risdate>2016</risdate><volume>40</volume><issue>9</issue><spage>999</spage><epage>1007</epage><pages>999-1007</pages><issn>1065-6995</issn><eissn>1095-8355</eissn><abstract>Mechanical strain plays an important role in bone formation and resorption during orthodontic tooth movement. The mechanism has not been fully studied, and the process becomes complex with increased amounts of periodontal patients seeking orthodontic care. Our aims were to elucidate the combined effects of proinflammatory cytokines and intermittent cyclic strain (ICS) on the osteogenic capacity of human periodontal ligament cells. Cultured human periodontal ligament cells were exposed to proinflammatory cytokines (interleukin‐1β 5 ng/mL and tumor necrosis factor‐α 10 ng/mL) for 1 and 5 days, and ICS (0.5 Hz, 12% elongation) was applied for 4 h per day. The autocrine of inflammatory cytokines was measured by enzyme‐linked immunosorbent assay. The expression of osteoblast markers runt‐related transcription factor 2 and rabbit collagen type I was determined using real‐time polymerase chain reaction and Western blot. The osteogenic capacity was also detected by alkaline phosphatase (ALP) staining, ALP activity, and alizarin red staining. We demonstrated that ICS impaired the osteogenic capacity of human periodontal ligament cells when incubated with proinflammatory cytokines, as evidenced by the low expression of ALP staining, low ALP activity, reduced alizarin red staining, and reduced osteoblast markers. These data, for the first time, suggest that ICS has a negative effect on the inductive inhibition of osteogenicity in human PDL cells mediated by proinflammatory cytokines.</abstract><cop>England</cop><pub>Blackwell Publishing Ltd</pub><pmid>27357508</pmid><doi>10.1002/cbin.10641</doi><tpages>9</tpages></addata></record> |
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| subjects | Adolescent Adult Cell Differentiation - physiology Cells, Cultured Cytokines Cytokines - immunology Cytokines - metabolism Female human periodontal ligament cells Humans ICS IL-1β Inflammation - metabolism Ligaments Male Orthodontics Osteoblasts - cytology Osteoblasts - metabolism Osteogenesis - physiology osteogenicity Periodontal Ligament - cytology Periodontal Ligament - immunology Periodontal Ligament - metabolism Periodontitis - metabolism Periodontitis - pathology proinflammatory cytokines Stress, Mechanical TNF-α Tooth Mobility - immunology Tooth Mobility - metabolism Young Adult |
| title | Combined effects of proinflammatory cytokines and intermittent cyclic mechanical strain in inhibiting osteogenicity in human periodontal ligament cells |
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