Detection and quantification of Leishmania infantum in naturally and experimentally infected animal samples

•QPCR strategy for specific detection of L. infantum parasites in animal samples.•Specific primer-probe for distinguishing between L. infantum and L. donovani species.•Approach for the diagnosis of canine visceral leishmaniasis due to L. infantum.•Parasite load quantification in symptomatic and asym...

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Veröffentlicht in:	Veterinary parasitology 2016-08, Vol.226, p.57-64
Hauptverfasser:	Losada-Barragán, Monica, Cavalcanti, Amanda, Umaña-Pérez, Adriana, Porrozzi, Renato, Cuervo-Escobar, Sergio, Vallejo, Andrés Felipe, Sánchez-Gómez, Myriam, Cuervo, Patricia
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence Cricetinae DNA Primers - chemistry DNA, Protozoan - chemistry Dog Diseases - parasitology Dogs Leishmania infantum - genetics Leishmania infantum - growth & development Leishmania infantum - isolation & purification Leishmaniasis, Visceral - parasitology Leishmaniasis, Visceral - veterinary Liver - parasitology Mice Mice, Inbred BALB C Real-Time Polymerase Chain Reaction Sensitivity and Specificity Spleen - parasitology
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