A low-temperature polygalacturonase from P. occitanis: characterization and application in juice clarification

•Purification of a new polygalacturonase after one MonoQ-FPLC purification step. ≡ PG2 was optimally active at 35°C and pH 6 with 85% of activity at pH7. ≡ DEPC strongly inhibited PG2 suggesting the implication of Histidine residue.•Molecular analysis of the gene supported the presence of His residu...

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Veröffentlicht in:	International journal of biological macromolecules 2016-10, Vol.91, p.158-164
Hauptverfasser:	Sassi, Azza Hadj, Tounsi, Hajer, Trigui-Lahiani, Hèla, Bouzouita, Rihab, Romdhane, Zamen Ben, Gargouri, Ali
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Clarification DEPC Endo-PGase Enzyme Stability Food Handling Fruit and Vegetable Juices Hydrogen-Ion Concentration Iodoacetamide - metabolism Metals - pharmacology Mutation Penicillium - enzymology Penicillium - genetics Polygalacturonase - chemistry Polygalacturonase - genetics Polygalacturonase - isolation & purification Polygalacturonase - metabolism Substrate Specificity Temperature
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