A Simple and Sensitive Method for Auramine O Detection Based on the Binding Interaction with Bovin Serum Albumin

A simple, rapid and effective method for auramine O (AO) detection was proposed by fluorescence and UV-Vis absorption spectroscopy. In the BR buffer system (pH 7.0), AO had a strong quenching ability to the fluorescence of bovin serum albumin (BSA) by dynamic quenching. In terms of the thermodynamic...

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Veröffentlicht in:	Analytical Sciences 2016/08/10, Vol.32(8), pp.819-824
Hauptverfasser:	YAN, Jingjing, HUANG, Xin, LIU, Shaopu, YANG, Jidong, YUAN, Yusheng, DUAN, Ruilin, ZHANG, Hui, HU, Xiaoli
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical Chemistry Auramine O Benzophenoneidum - analysis Benzophenoneidum - metabolism bovin serum albumin Chemistry fluorescence Fluorescence Resonance Energy Transfer FRET Hydrogen-Ion Concentration Hydrophobic and Hydrophilic Interactions Limit of Detection Original Papers Osmolar Concentration Protein Binding Serum Albumin, Bovine - metabolism Spectrometry, Fluorescence
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