Site-selective incorporation and ligation of protein aldehydes

Site-selective incorporation and ligation of protein aldehydes

The incorporation of aldehyde handles into proteins, and subsequent chemical reactions thereof, is rapidly proving to be an effective way of generating homogeneous, covalently linked protein constructs that can display a vast array of functionality. In this review, we discuss methods for introducing...

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Veröffentlicht in:Organic & biomolecular chemistry 2016-01, Vol.14 (32), p.7622-7638
Hauptverfasser: Spears, Richard J, Fascione, Martin A
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Sprache:eng
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Aldehydes - chemistry
Models, Molecular
Molecular Structure
Proteins - chemistry
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creator Spears, Richard J
Fascione, Martin A
description The incorporation of aldehyde handles into proteins, and subsequent chemical reactions thereof, is rapidly proving to be an effective way of generating homogeneous, covalently linked protein constructs that can display a vast array of functionality. In this review, we discuss methods for introducing aldehydes into target proteins, and summarise the ligation strategies for site-selective modification of proteins containing this class of functional handles. The incorporation of aldehyde handles into proteins, and subsequent chemical reactions thereof, is rapidly proving to be an effective way of generating homogeneous, covalently linked protein constructs that can display a vast array of functionality.
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source MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection
subjects Aldehydes - chemistry
Models, Molecular
Molecular Structure
Proteins - chemistry
title Site-selective incorporation and ligation of protein aldehydes
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