Alteration of the immune status of umbilical cord mesenchymal stem cells stimulated by TLR1/2 agonist, Pam3Csk
Mesenchymal stem cells (MSCs) have been widely used in clinical trials due to their multiple differentiation ability, low immunogenicity and immunosuppressant effects on immune response. However, accumulating evidence has indicated that MSCs may stimulate in vivo immune responses and result in the d...
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| Veröffentlicht in: | Molecular medicine reports 2016-09, Vol.14 (3), p.2206-2212 |
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| creator | Wang, Pingxi Zeng, Fanwei He, Lina Wang, Jin Zhang, Tingjiu Zhang, Dong |
| description | Mesenchymal stem cells (MSCs) have been widely used in clinical trials due to their multiple differentiation ability, low immunogenicity and immunosuppressant effects on immune response. However, accumulating evidence has indicated that MSCs may stimulate in vivo immune responses and result in the disappearance of MSCs following engrafting. Toll-like receptors (TLRs) are important in immune response induction against invaded pathogens, however, the function of TLRs in regulating the immune status of MSCs has been seldom reported. The present stimulated umbilical cord (UC) MSCs by treatment with the TLR1/2 agonist, Pam3Csk, the to determine whether activation of TLR1/2 signaling alters the immune status of UCMSCs. The results indicated that activation of TLR1/2 increased the proliferation of peripheral blood mononuclear cells (PBMCs) and the production of lactate dehydrogenase in a PBMC-MSC co-culture system. The study also demonstrated that Pam3Csk induced the secretion of pro-inflammatory molecules, and increased the expression levels of cytokine and chemokines in UCMSCs. Flow cytometry analysis indicated that the levels of surface co-stimulators, CD80 and CD86, were increased on UCMSCs in the presence of Pam3Csk, whereas activation of TLR1/2 exerted no observable effect on the differentiation abilities of UCMSCs. The results of the current study indicated that activation of TLR1/2 signaling may alter the immune status of UCMSCs, however, further mechanistic research is required in future studies. |
| doi_str_mv | 10.3892/mmr.2016.5520 |
| format | Article |
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However, accumulating evidence has indicated that MSCs may stimulate in vivo immune responses and result in the disappearance of MSCs following engrafting. Toll-like receptors (TLRs) are important in immune response induction against invaded pathogens, however, the function of TLRs in regulating the immune status of MSCs has been seldom reported. The present stimulated umbilical cord (UC) MSCs by treatment with the TLR1/2 agonist, Pam3Csk, the to determine whether activation of TLR1/2 signaling alters the immune status of UCMSCs. The results indicated that activation of TLR1/2 increased the proliferation of peripheral blood mononuclear cells (PBMCs) and the production of lactate dehydrogenase in a PBMC-MSC co-culture system. The study also demonstrated that Pam3Csk induced the secretion of pro-inflammatory molecules, and increased the expression levels of cytokine and chemokines in UCMSCs. Flow cytometry analysis indicated that the levels of surface co-stimulators, CD80 and CD86, were increased on UCMSCs in the presence of Pam3Csk, whereas activation of TLR1/2 exerted no observable effect on the differentiation abilities of UCMSCs. The results of the current study indicated that activation of TLR1/2 signaling may alter the immune status of UCMSCs, however, further mechanistic research is required in future studies.</description><identifier>ISSN: 1791-2997</identifier><identifier>EISSN: 1791-3004</identifier><identifier>DOI: 10.3892/mmr.2016.5520</identifier><identifier>PMID: 27431529</identifier><language>eng</language><publisher>Greece: D.A. Spandidos</publisher><subject>CD80 antigen ; CD86 antigen ; Cell Communication ; Cell culture ; Cell Proliferation ; Cells, Cultured ; Chemokines ; Clinical trials ; Coculture Techniques ; Cytokines - metabolism ; Cytotoxicity ; Dehydrogenases ; Flow cytometry ; Gene expression ; Genetic aspects ; Growth ; Humans ; Immune response ; Immune status ; Immunogenicity ; Immunoglobulins ; Immunomodulation - drug effects ; Inflammation ; Inflammation Mediators - metabolism ; L-Lactate dehydrogenase ; Lactic acid ; Leukocytes (mononuclear) ; Leukocytes, Mononuclear - drug effects ; Leukocytes, Mononuclear - metabolism ; Ligands ; Lipopeptides - pharmacology ; Mesenchymal stem cells ; Mesenchymal Stromal Cells - cytology ; Mesenchymal Stromal Cells - drug effects ; Mesenchymal Stromal Cells - physiology ; Mesenchyme ; Pam3Csk ; Peripheral blood mononuclear cells ; Polymerase chain reaction ; pro-inflammatory molecules ; Properties ; Proteins ; Regulation ; Signal Transduction - drug effects ; Stem cells ; TLR1 protein ; Toll-Like Receptor 1 - agonists ; Toll-like receptor 1/2 ; Toll-Like Receptor 2 - agonists ; Toll-like receptors ; Tumor necrosis factor-TNF ; Umbilical cord ; Umbilical Cord - cytology ; umbilical cord mesenchymal stem cells</subject><ispartof>Molecular medicine reports, 2016-09, Vol.14 (3), p.2206-2212</ispartof><rights>Copyright © 2016, Spandidos Publications</rights><rights>COPYRIGHT 2016 Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c525t-a9af1c51613c7208c75fe7433dc7d2ce6f384255df7bcc6a30b554bc25c2a6783</citedby><cites>FETCH-LOGICAL-c525t-a9af1c51613c7208c75fe7433dc7d2ce6f384255df7bcc6a30b554bc25c2a6783</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,5556,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27431529$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Pingxi</creatorcontrib><creatorcontrib>Zeng, Fanwei</creatorcontrib><creatorcontrib>He, Lina</creatorcontrib><creatorcontrib>Wang, Jin</creatorcontrib><creatorcontrib>Zhang, Tingjiu</creatorcontrib><creatorcontrib>Zhang, Dong</creatorcontrib><title>Alteration of the immune status of umbilical cord mesenchymal stem cells stimulated by TLR1/2 agonist, Pam3Csk</title><title>Molecular medicine reports</title><addtitle>Mol Med Rep</addtitle><description>Mesenchymal stem cells (MSCs) have been widely used in clinical trials due to their multiple differentiation ability, low immunogenicity and immunosuppressant effects on immune response. However, accumulating evidence has indicated that MSCs may stimulate in vivo immune responses and result in the disappearance of MSCs following engrafting. Toll-like receptors (TLRs) are important in immune response induction against invaded pathogens, however, the function of TLRs in regulating the immune status of MSCs has been seldom reported. The present stimulated umbilical cord (UC) MSCs by treatment with the TLR1/2 agonist, Pam3Csk, the to determine whether activation of TLR1/2 signaling alters the immune status of UCMSCs. The results indicated that activation of TLR1/2 increased the proliferation of peripheral blood mononuclear cells (PBMCs) and the production of lactate dehydrogenase in a PBMC-MSC co-culture system. The study also demonstrated that Pam3Csk induced the secretion of pro-inflammatory molecules, and increased the expression levels of cytokine and chemokines in UCMSCs. Flow cytometry analysis indicated that the levels of surface co-stimulators, CD80 and CD86, were increased on UCMSCs in the presence of Pam3Csk, whereas activation of TLR1/2 exerted no observable effect on the differentiation abilities of UCMSCs. The results of the current study indicated that activation of TLR1/2 signaling may alter the immune status of UCMSCs, however, further mechanistic research is required in future studies.</description><subject>CD80 antigen</subject><subject>CD86 antigen</subject><subject>Cell Communication</subject><subject>Cell culture</subject><subject>Cell Proliferation</subject><subject>Cells, Cultured</subject><subject>Chemokines</subject><subject>Clinical trials</subject><subject>Coculture Techniques</subject><subject>Cytokines - metabolism</subject><subject>Cytotoxicity</subject><subject>Dehydrogenases</subject><subject>Flow cytometry</subject><subject>Gene expression</subject><subject>Genetic aspects</subject><subject>Growth</subject><subject>Humans</subject><subject>Immune response</subject><subject>Immune status</subject><subject>Immunogenicity</subject><subject>Immunoglobulins</subject><subject>Immunomodulation - drug effects</subject><subject>Inflammation</subject><subject>Inflammation Mediators - metabolism</subject><subject>L-Lactate dehydrogenase</subject><subject>Lactic acid</subject><subject>Leukocytes (mononuclear)</subject><subject>Leukocytes, Mononuclear - drug effects</subject><subject>Leukocytes, Mononuclear - metabolism</subject><subject>Ligands</subject><subject>Lipopeptides - pharmacology</subject><subject>Mesenchymal stem cells</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Mesenchymal Stromal Cells - drug effects</subject><subject>Mesenchymal Stromal Cells - physiology</subject><subject>Mesenchyme</subject><subject>Pam3Csk</subject><subject>Peripheral blood mononuclear cells</subject><subject>Polymerase chain reaction</subject><subject>pro-inflammatory molecules</subject><subject>Properties</subject><subject>Proteins</subject><subject>Regulation</subject><subject>Signal Transduction - drug effects</subject><subject>Stem cells</subject><subject>TLR1 protein</subject><subject>Toll-Like Receptor 1 - agonists</subject><subject>Toll-like receptor 1/2</subject><subject>Toll-Like Receptor 2 - agonists</subject><subject>Toll-like receptors</subject><subject>Tumor necrosis factor-TNF</subject><subject>Umbilical cord</subject><subject>Umbilical Cord - cytology</subject><subject>umbilical cord mesenchymal stem cells</subject><issn>1791-2997</issn><issn>1791-3004</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNptkc2LFDEQxRtR3HX16FUCHvRgzyaVTrpzHAa_YECR9RzSSXo3aycZk_Rh_nvTzLioSB2qePzq8eA1zUuCN3QQcO192gAmfMMY4EfNJekFaSnG3ePzDUL0F82znO8x5gyYeNpcQN9RwkBcNmE7F5tUcTGgOKFyZ5HzfgkW5aLKkldx8aObnVYz0jEZ5G22Qd8dfRVysR5pO8-5ns4vsyrWoPGIbvbfyDUgdRuDy-Ud-qo83eUfz5snk5qzfXHeV833D-9vdp_a_ZePn3fbfatrwtIqoSaiGeGE6h7woHs22ZqZGt0b0JZPdOiAMTP1o9ZcUTwy1o0amAbF-4FeNW9PvocUfy42F-ldXnOqYOOSJRkIpoxS4BV9_Q96H5cUajpJBIWuHzD_g7pVs5UuTLEkpVdTue04iAE6Jiq1-Q9Vx1jvdAx2clX_66E9PegUc052kofkvEpHSbBc-5W1X7n2K9d-K__qHHYZvTUP9O9CK_DmBOSDCsaZmB-Y6tSSrsW0BcCc_gJ766uV</recordid><startdate>20160901</startdate><enddate>20160901</enddate><creator>Wang, Pingxi</creator><creator>Zeng, Fanwei</creator><creator>He, Lina</creator><creator>Wang, Jin</creator><creator>Zhang, Tingjiu</creator><creator>Zhang, Dong</creator><general>D.A. Spandidos</general><general>Spandidos Publications</general><general>Spandidos Publications UK Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20160901</creationdate><title>Alteration of the immune status of umbilical cord mesenchymal stem cells stimulated by TLR1/2 agonist, Pam3Csk</title><author>Wang, Pingxi ; Zeng, Fanwei ; He, Lina ; Wang, Jin ; Zhang, Tingjiu ; Zhang, Dong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c525t-a9af1c51613c7208c75fe7433dc7d2ce6f384255df7bcc6a30b554bc25c2a6783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>CD80 antigen</topic><topic>CD86 antigen</topic><topic>Cell Communication</topic><topic>Cell culture</topic><topic>Cell Proliferation</topic><topic>Cells, Cultured</topic><topic>Chemokines</topic><topic>Clinical trials</topic><topic>Coculture Techniques</topic><topic>Cytokines - metabolism</topic><topic>Cytotoxicity</topic><topic>Dehydrogenases</topic><topic>Flow cytometry</topic><topic>Gene expression</topic><topic>Genetic aspects</topic><topic>Growth</topic><topic>Humans</topic><topic>Immune response</topic><topic>Immune status</topic><topic>Immunogenicity</topic><topic>Immunoglobulins</topic><topic>Immunomodulation - drug effects</topic><topic>Inflammation</topic><topic>Inflammation Mediators - metabolism</topic><topic>L-Lactate dehydrogenase</topic><topic>Lactic acid</topic><topic>Leukocytes (mononuclear)</topic><topic>Leukocytes, Mononuclear - drug effects</topic><topic>Leukocytes, Mononuclear - metabolism</topic><topic>Ligands</topic><topic>Lipopeptides - pharmacology</topic><topic>Mesenchymal stem cells</topic><topic>Mesenchymal Stromal Cells - cytology</topic><topic>Mesenchymal Stromal Cells - drug effects</topic><topic>Mesenchymal Stromal Cells - physiology</topic><topic>Mesenchyme</topic><topic>Pam3Csk</topic><topic>Peripheral blood mononuclear cells</topic><topic>Polymerase chain reaction</topic><topic>pro-inflammatory molecules</topic><topic>Properties</topic><topic>Proteins</topic><topic>Regulation</topic><topic>Signal Transduction - drug effects</topic><topic>Stem cells</topic><topic>TLR1 protein</topic><topic>Toll-Like Receptor 1 - agonists</topic><topic>Toll-like receptor 1/2</topic><topic>Toll-Like Receptor 2 - agonists</topic><topic>Toll-like receptors</topic><topic>Tumor necrosis factor-TNF</topic><topic>Umbilical cord</topic><topic>Umbilical Cord - cytology</topic><topic>umbilical cord mesenchymal stem cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Pingxi</creatorcontrib><creatorcontrib>Zeng, Fanwei</creatorcontrib><creatorcontrib>He, Lina</creatorcontrib><creatorcontrib>Wang, Jin</creatorcontrib><creatorcontrib>Zhang, Tingjiu</creatorcontrib><creatorcontrib>Zhang, Dong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular medicine reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Pingxi</au><au>Zeng, Fanwei</au><au>He, Lina</au><au>Wang, Jin</au><au>Zhang, Tingjiu</au><au>Zhang, Dong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Alteration of the immune status of umbilical cord mesenchymal stem cells stimulated by TLR1/2 agonist, Pam3Csk</atitle><jtitle>Molecular medicine reports</jtitle><addtitle>Mol Med Rep</addtitle><date>2016-09-01</date><risdate>2016</risdate><volume>14</volume><issue>3</issue><spage>2206</spage><epage>2212</epage><pages>2206-2212</pages><issn>1791-2997</issn><eissn>1791-3004</eissn><abstract>Mesenchymal stem cells (MSCs) have been widely used in clinical trials due to their multiple differentiation ability, low immunogenicity and immunosuppressant effects on immune response. However, accumulating evidence has indicated that MSCs may stimulate in vivo immune responses and result in the disappearance of MSCs following engrafting. Toll-like receptors (TLRs) are important in immune response induction against invaded pathogens, however, the function of TLRs in regulating the immune status of MSCs has been seldom reported. The present stimulated umbilical cord (UC) MSCs by treatment with the TLR1/2 agonist, Pam3Csk, the to determine whether activation of TLR1/2 signaling alters the immune status of UCMSCs. The results indicated that activation of TLR1/2 increased the proliferation of peripheral blood mononuclear cells (PBMCs) and the production of lactate dehydrogenase in a PBMC-MSC co-culture system. The study also demonstrated that Pam3Csk induced the secretion of pro-inflammatory molecules, and increased the expression levels of cytokine and chemokines in UCMSCs. Flow cytometry analysis indicated that the levels of surface co-stimulators, CD80 and CD86, were increased on UCMSCs in the presence of Pam3Csk, whereas activation of TLR1/2 exerted no observable effect on the differentiation abilities of UCMSCs. The results of the current study indicated that activation of TLR1/2 signaling may alter the immune status of UCMSCs, however, further mechanistic research is required in future studies.</abstract><cop>Greece</cop><pub>D.A. Spandidos</pub><pmid>27431529</pmid><doi>10.3892/mmr.2016.5520</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | Spandidos Publications Journals; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | CD80 antigen CD86 antigen Cell Communication Cell culture Cell Proliferation Cells, Cultured Chemokines Clinical trials Coculture Techniques Cytokines - metabolism Cytotoxicity Dehydrogenases Flow cytometry Gene expression Genetic aspects Growth Humans Immune response Immune status Immunogenicity Immunoglobulins Immunomodulation - drug effects Inflammation Inflammation Mediators - metabolism L-Lactate dehydrogenase Lactic acid Leukocytes (mononuclear) Leukocytes, Mononuclear - drug effects Leukocytes, Mononuclear - metabolism Ligands Lipopeptides - pharmacology Mesenchymal stem cells Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - drug effects Mesenchymal Stromal Cells - physiology Mesenchyme Pam3Csk Peripheral blood mononuclear cells Polymerase chain reaction pro-inflammatory molecules Properties Proteins Regulation Signal Transduction - drug effects Stem cells TLR1 protein Toll-Like Receptor 1 - agonists Toll-like receptor 1/2 Toll-Like Receptor 2 - agonists Toll-like receptors Tumor necrosis factor-TNF Umbilical cord Umbilical Cord - cytology umbilical cord mesenchymal stem cells |
| title | Alteration of the immune status of umbilical cord mesenchymal stem cells stimulated by TLR1/2 agonist, Pam3Csk |
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