In vitro tetraploid induction and generation of tetraploids from mixoploids in hop (Humulus lupulus L.)

This paper describes an efficient colchicine-mediated technique for the in vitro induction of hop tetraploids and its confirmation by flow cytometry. A window of conditions generated a high percentage (>20%) of tetraploid induction, with the highest induction (25.6%) achieved with 0.05% colchicin...

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Veröffentlicht in:	Plant cell reports 2001-09, Vol.20 (6), p.489-495
Hauptverfasser:	ROY, A. T, LEGGETT, G, KOUTOULIS, A
Format:	Artikel
Sprache:	eng
Schlagworte:	Agronomy. Soil science and plant productions Anther and ovule culture, utilization for haploid production Biological and medical sciences Biotechnology Callus Colchicine Eukaryotic cell cultures Flow cytometry Fundamental and applied biological sciences. Psychology Genetics and breeding of economic plants Genomes Haploidy, in vitro culture applications, somatic hybrids Hops Humulus lupulus Leaves Methods. Procedures. Technologies Parameter identification Plant breeding: fundamental aspects and methodology Plant cells and fungal cells Shoots Stomata tetraploidy
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