In vitro tetraploid induction and generation of tetraploids from mixoploids in hop (Humulus lupulus L.)
This paper describes an efficient colchicine-mediated technique for the in vitro induction of hop tetraploids and its confirmation by flow cytometry. A window of conditions generated a high percentage (>20%) of tetraploid induction, with the highest induction (25.6%) achieved with 0.05% colchicin...
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| Veröffentlicht in: | Plant cell reports 2001-09, Vol.20 (6), p.489-495 |
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| creator | ROY, A. T LEGGETT, G KOUTOULIS, A |
| description | This paper describes an efficient colchicine-mediated technique for the in vitro induction of hop tetraploids and its confirmation by flow cytometry. A window of conditions generated a high percentage (>20%) of tetraploid induction, with the highest induction (25.6%) achieved with 0.05% colchicine for 48 h. Colchicine-induced tetraploids remained stable after 6 months in soil. Leaf characteristics of diploid and tetraploid hops were compared, and it was determined that stomatal length and width are suitable parameters for identifying putative hop tetraploids. As well as generating tetraploids, this technique generates mixoploid hops. Calli, derived from mixoploid leaves, were induced to form shoot buds and shoots. Individual shoots were classed as diploid, mixoploid or tetraploid after screening by flow cytometry. This callus-based technique can be employed when a genome-doubling agent generates mixoploids but fails to generate tetraploids. |
| doi_str_mv | 10.1007/s002990100364 |
| format | Article |
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This callus-based technique can be employed when a genome-doubling agent generates mixoploids but fails to generate tetraploids.</description><identifier>ISSN: 0721-7714</identifier><identifier>EISSN: 1432-203X</identifier><identifier>DOI: 10.1007/s002990100364</identifier><identifier>CODEN: PCRPD8</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Agronomy. Soil science and plant productions ; Anther and ovule culture, utilization for haploid production ; Biological and medical sciences ; Biotechnology ; Callus ; Colchicine ; Eukaryotic cell cultures ; Flow cytometry ; Fundamental and applied biological sciences. Psychology ; Genetics and breeding of economic plants ; Genomes ; Haploidy, in vitro culture applications, somatic hybrids ; Hops ; Humulus lupulus ; Leaves ; Methods. Procedures. Technologies ; Parameter identification ; Plant breeding: fundamental aspects and methodology ; Plant cells and fungal cells ; Shoots ; Stomata ; tetraploidy</subject><ispartof>Plant cell reports, 2001-09, Vol.20 (6), p.489-495</ispartof><rights>2002 INIST-CNRS</rights><rights>Plant Cell Reports is a copyright of Springer, (2001). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c390t-1dc2af7cb11392aa5b516f40bade00bc45690a0e1a3b936429fd776c53c093e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14057815$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>ROY, A. 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| subjects | Agronomy. Soil science and plant productions Anther and ovule culture, utilization for haploid production Biological and medical sciences Biotechnology Callus Colchicine Eukaryotic cell cultures Flow cytometry Fundamental and applied biological sciences. Psychology Genetics and breeding of economic plants Genomes Haploidy, in vitro culture applications, somatic hybrids Hops Humulus lupulus Leaves Methods. Procedures. Technologies Parameter identification Plant breeding: fundamental aspects and methodology Plant cells and fungal cells Shoots Stomata tetraploidy |
| title | In vitro tetraploid induction and generation of tetraploids from mixoploids in hop (Humulus lupulus L.) |
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