Colorimetric assay for Lindane dechlorination by bacteria

A colorimetric microtitre plate-based assay that detects haloalkane dehalogenase activity was modified to detect dechlorination of γ-hexachlorocyclohexane (Lindane). Dechlorination is indicated by the colour change of phenol red from red to yellow, in a weakly buffered solution, as the solution beco...

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Veröffentlicht in:	Journal of microbiological methods 2001-11, Vol.47 (2), p.181-188
Hauptverfasser:	Phillips, Theresa M, Seech, Alan G, Lee, Hung, Trevors, Jack T
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteria Bacteria - metabolism Bacteriological methods and techniques used in bacteriology Bacteriology Biodegradation, Environmental Biological and medical sciences Chlorides - metabolism Colorimetric Colorimetry - methods Dechlorination Enzyme kinetics Ethylene Dichlorides - metabolism Fundamental and applied biological sciences. Psychology g-Hexachlorocyclohexane Insecticides - chemistry Insecticides - metabolism Isolate screening Kinetics Lindane Lindane - chemistry Lindane - metabolism Microbiology Oxidation-Reduction phenol red phenolsulfonphthalein Pseudomonas - metabolism Systematics
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