Cu super(2+)-selective ratiometric fluorescence signaling probe based on the hydrolysis of dansylhydrazine

A simple Cu super(2+)-selective ratiometric fluorescence signaling probe based on the hydrolysis of dansylhydrazine was developed. Dansylhydrazine 1 showed prominent ratiometric fluorescence signaling behavior toward Cu super(2+) ions in a 50% aqueous (pH 7.4) acetonitrile solution. The ratiometric signaling behavior was due to the Cu super(2+)-assisted hydrolysis of the yellow-colored fluorescent dansylhydrazine to strongly blue-fluorescent dansyl acid. The Cu super(2+)-selective signaling of probe 1 was not affected by the presence of biologically important metal ions. The ratiometric signaling of Cu super(2+) by 1 was possible with a detection limit of 6.0 10 super(-8) M (3.8 ppb). In addition, the signaling of Cu super(2+) in biological samples using synthetic urine was also tested. InChIKeys * WAZWAZXWPDTCKG-UHFFFAOYSA-N * KPQYDVAFRDWIBW-UHFFFAOYSA-N