Recovery of cryopreserved silver birch shoot tips is affected by the pre-freezing age of the cultures and ammonium substitution
The aim of the present study was to improve the cryopreservation protocol for silver birch (Betula pendula Roth) in vitro shoot tips. The recovery of shoot tips derived from young cultures (shoot tips excised from less than 20-month-old cultures) was significantly better than that from old cultures...
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| Veröffentlicht in: | Plant cell reports 2001-06, Vol.20 (4), p.354-360 |
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| creator | Ryynaenen, L Haeggman, H |
| description | The aim of the present study was to improve the cryopreservation protocol for silver birch (Betula pendula Roth) in vitro shoot tips. The recovery of shoot tips derived from young cultures (shoot tips excised from less than 20-month-old cultures) was significantly better than that from old cultures (shoot tips excised from cultures at least 55 months old) when the standard protocol, i.e. the slow-cooling procedure with PGD as cryoprotectant, was used. The recovery of shoot tips of both ages was, however, improved by substituting KNO3 for ammonium during cold hardening and post-thaw cultivation. The substitution of ammonium with KNO3 improved recovery in most of the genotypes, indicating the possibility of developing a cryopreservation protocol applicable to several genotypes. |
| doi_str_mv | 10.1007/s002990100336 |
| format | Article |
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The recovery of shoot tips derived from young cultures (shoot tips excised from less than 20-month-old cultures) was significantly better than that from old cultures (shoot tips excised from cultures at least 55 months old) when the standard protocol, i.e. the slow-cooling procedure with PGD as cryoprotectant, was used. The recovery of shoot tips of both ages was, however, improved by substituting KNO3 for ammonium during cold hardening and post-thaw cultivation. The substitution of ammonium with KNO3 improved recovery in most of the genotypes, indicating the possibility of developing a cryopreservation protocol applicable to several genotypes.</description><identifier>ISSN: 0721-7714</identifier><identifier>EISSN: 1432-203X</identifier><identifier>DOI: 10.1007/s002990100336</identifier><identifier>CODEN: PCRPD8</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Ammonium ; Biological and medical sciences ; Biotechnology ; Cold treatment ; Cryopreservation ; Cryoprotectors ; Cultivation ; Eukaryotic cell cultures ; Freezing ; Fundamental and applied biological sciences. Psychology ; Genotypes ; Leaves ; Methods. Procedures. Technologies ; Miscellaneous ; Plant cells and fungal cells ; Plant growth ; Recovery ; Substitutes ; Tips</subject><ispartof>Plant cell reports, 2001-06, Vol.20 (4), p.354-360</ispartof><rights>2001 INIST-CNRS</rights><rights>Plant Cell Reports is a copyright of Springer, (2001). 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The substitution of ammonium with KNO3 improved recovery in most of the genotypes, indicating the possibility of developing a cryopreservation protocol applicable to several genotypes.</description><subject>Ammonium</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cold treatment</subject><subject>Cryopreservation</subject><subject>Cryoprotectors</subject><subject>Cultivation</subject><subject>Eukaryotic cell cultures</subject><subject>Freezing</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genotypes</subject><subject>Leaves</subject><subject>Methods. Procedures. 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| ispartof | Plant cell reports, 2001-06, Vol.20 (4), p.354-360 |
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| subjects | Ammonium Biological and medical sciences Biotechnology Cold treatment Cryopreservation Cryoprotectors Cultivation Eukaryotic cell cultures Freezing Fundamental and applied biological sciences. Psychology Genotypes Leaves Methods. Procedures. Technologies Miscellaneous Plant cells and fungal cells Plant growth Recovery Substitutes Tips |
| title | Recovery of cryopreserved silver birch shoot tips is affected by the pre-freezing age of the cultures and ammonium substitution |
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