Monitoring of cholesterol oxidation in a lipid bilayer membrane using streptolysin O as a sensing and signal transduction element
[Display omitted] •A fluorometric method for monitoring chol oxidation in a lipid bilayer is described based on the pore forming ability of SLO.•The 3β-OH configuration of sterol in lipid bilayers is important for SLO to form a nanopore.•The P30min value decreased almost linearly with the mole fract...
Gespeichert in:
| Veröffentlicht in: | Journal of pharmaceutical and biomedical analysis 2016-09, Vol.128, p.455-461 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 461 |
|---|---|
| container_issue | |
| container_start_page | 455 |
| container_title | Journal of pharmaceutical and biomedical analysis |
| container_volume | 128 |
| creator | Shoji, Atsushi Ikeya, Kana Aoyagi, Miki Takatsuji, Ryutaro Yanagida, Akio Shibusawa, Yoichi Sugawara, Masao |
| description | [Display omitted]
•A fluorometric method for monitoring chol oxidation in a lipid bilayer is described based on the pore forming ability of SLO.•The 3β-OH configuration of sterol in lipid bilayers is important for SLO to form a nanopore.•The P30min value decreased almost linearly with the mole fraction of 4-cholesten-3-one in immobilized liposomes.•The progress of enzymatic chol oxidation in liposomes was detected by utilizing the SLO.
Streptolysin O (SLO), which recognizes sterols and forms nanopores in lipid membranes, is proposed as a sensing element for monitoring cholesterol oxidation in a lipid bilayer. The structural requirements of eight sterols for forming nanopores by SLO confirmed that a free 3-OH group in the β-configuration of sterols is required for recognition by SLO in a lipid bilayer. The extent of nanopore formation by SLO in lipid bilayers increased in the order of cholestanol |
| doi_str_mv | 10.1016/j.jpba.2016.06.009 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1809047634</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0731708516303260</els_id><sourcerecordid>1809047634</sourcerecordid><originalsourceid>FETCH-LOGICAL-c422t-a1b340f42b8d0f78fa758c5ddd2c742d956b1d373df67ea2da332bd1a4d9b583</originalsourceid><addsrcrecordid>eNp9kEuLFDEUhYMoTjv6B1xIlm6qzasqVeBGhvEBI7OZhbuQx60xTSopk5TYS_-56enRpXDh3pDzHTgHodeU7Cmhw7vD_rAavWft3pM2ZHqCdnSUvGOD-PYU7YjktJNk7C_Qi1IOhJCeTuI5umCSD0z0cod-f03R15R9vMdpxvZ7ClAq5BRw-uWdrj5F7CPWOPjVO2x80EfIeIHFZB0Bb-WElpphrSkc2wvfYl0aUCA-_OnocPH3UQdcG1LcZh9cIcACsb5Ez2YdCrx63Jfo7uP13dXn7ub205erDzedFYzVTlPDBZkFM6MjsxxnLfvR9s45ZqVgbuoHQx2X3M2DBM2c5pwZR7Vwk-lHfonenm3XnH5sLaNafLEQQguRtqLoSCYi5MBFk7Kz1OZUSoZZrdkvOh8VJerUvDqoU_Pq1LwibcjUoDeP_ptZwP1D_lbdBO_PAmghf3rIqlgP0YLzGWxVLvn_-f8BKyOYCA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1809047634</pqid></control><display><type>article</type><title>Monitoring of cholesterol oxidation in a lipid bilayer membrane using streptolysin O as a sensing and signal transduction element</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Shoji, Atsushi ; Ikeya, Kana ; Aoyagi, Miki ; Takatsuji, Ryutaro ; Yanagida, Akio ; Shibusawa, Yoichi ; Sugawara, Masao</creator><creatorcontrib>Shoji, Atsushi ; Ikeya, Kana ; Aoyagi, Miki ; Takatsuji, Ryutaro ; Yanagida, Akio ; Shibusawa, Yoichi ; Sugawara, Masao</creatorcontrib><description>[Display omitted]
•A fluorometric method for monitoring chol oxidation in a lipid bilayer is described based on the pore forming ability of SLO.•The 3β-OH configuration of sterol in lipid bilayers is important for SLO to form a nanopore.•The P30min value decreased almost linearly with the mole fraction of 4-cholesten-3-one in immobilized liposomes.•The progress of enzymatic chol oxidation in liposomes was detected by utilizing the SLO.
Streptolysin O (SLO), which recognizes sterols and forms nanopores in lipid membranes, is proposed as a sensing element for monitoring cholesterol oxidation in a lipid bilayer. The structural requirements of eight sterols for forming nanopores by SLO confirmed that a free 3-OH group in the β-configuration of sterols is required for recognition by SLO in a lipid bilayer. The extent of nanopore formation by SLO in lipid bilayers increased in the order of cholestanol<cholesterol<25-OH cholesterol and in a sterol concentration-dependent manner. The immobilization of liposomes consisting of PC, cholesterol and 4-cholesten-3-one exhibited a linear relationship between calcein permeability and the molar ratio of cholesterol and 4-cholesten-3-one. The SLO-based method was successfully applied for monitoring of cholesterol oxidase–mediated oxidation of cholesterol in a lipid bilayer. The potential of the SLO nanopore-based method for monitoring cholesterol oxidation in a lipid bilayer by other oxidative enzymes is also discussed.</description><identifier>ISSN: 0731-7085</identifier><identifier>EISSN: 1873-264X</identifier><identifier>DOI: 10.1016/j.jpba.2016.06.009</identifier><identifier>PMID: 27362457</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Bacterial Proteins - analysis ; Bacterial Proteins - chemistry ; Bacterial Proteins - metabolism ; Cholesterol ; Cholesterol - analysis ; Cholesterol - metabolism ; Cholesterol oxidase ; Cholesterol Oxidase - metabolism ; Fluoresceins - chemistry ; Fluorometric permeability assay ; Immobilized liposome membrane ; Lipid Bilayers - chemistry ; Liposomes - chemistry ; Nanopores ; Oxidation-Reduction ; Signal Transduction ; Sterols - chemistry ; Sterols - metabolism ; Streptolysin O ; Streptolysins - analysis ; Streptolysins - chemistry ; Streptolysins - metabolism</subject><ispartof>Journal of pharmaceutical and biomedical analysis, 2016-09, Vol.128, p.455-461</ispartof><rights>2016 Elsevier B.V.</rights><rights>Copyright © 2016 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-a1b340f42b8d0f78fa758c5ddd2c742d956b1d373df67ea2da332bd1a4d9b583</citedby><cites>FETCH-LOGICAL-c422t-a1b340f42b8d0f78fa758c5ddd2c742d956b1d373df67ea2da332bd1a4d9b583</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jpba.2016.06.009$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27362457$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shoji, Atsushi</creatorcontrib><creatorcontrib>Ikeya, Kana</creatorcontrib><creatorcontrib>Aoyagi, Miki</creatorcontrib><creatorcontrib>Takatsuji, Ryutaro</creatorcontrib><creatorcontrib>Yanagida, Akio</creatorcontrib><creatorcontrib>Shibusawa, Yoichi</creatorcontrib><creatorcontrib>Sugawara, Masao</creatorcontrib><title>Monitoring of cholesterol oxidation in a lipid bilayer membrane using streptolysin O as a sensing and signal transduction element</title><title>Journal of pharmaceutical and biomedical analysis</title><addtitle>J Pharm Biomed Anal</addtitle><description>[Display omitted]
•A fluorometric method for monitoring chol oxidation in a lipid bilayer is described based on the pore forming ability of SLO.•The 3β-OH configuration of sterol in lipid bilayers is important for SLO to form a nanopore.•The P30min value decreased almost linearly with the mole fraction of 4-cholesten-3-one in immobilized liposomes.•The progress of enzymatic chol oxidation in liposomes was detected by utilizing the SLO.
Streptolysin O (SLO), which recognizes sterols and forms nanopores in lipid membranes, is proposed as a sensing element for monitoring cholesterol oxidation in a lipid bilayer. The structural requirements of eight sterols for forming nanopores by SLO confirmed that a free 3-OH group in the β-configuration of sterols is required for recognition by SLO in a lipid bilayer. The extent of nanopore formation by SLO in lipid bilayers increased in the order of cholestanol<cholesterol<25-OH cholesterol and in a sterol concentration-dependent manner. The immobilization of liposomes consisting of PC, cholesterol and 4-cholesten-3-one exhibited a linear relationship between calcein permeability and the molar ratio of cholesterol and 4-cholesten-3-one. The SLO-based method was successfully applied for monitoring of cholesterol oxidase–mediated oxidation of cholesterol in a lipid bilayer. The potential of the SLO nanopore-based method for monitoring cholesterol oxidation in a lipid bilayer by other oxidative enzymes is also discussed.</description><subject>Bacterial Proteins - analysis</subject><subject>Bacterial Proteins - chemistry</subject><subject>Bacterial Proteins - metabolism</subject><subject>Cholesterol</subject><subject>Cholesterol - analysis</subject><subject>Cholesterol - metabolism</subject><subject>Cholesterol oxidase</subject><subject>Cholesterol Oxidase - metabolism</subject><subject>Fluoresceins - chemistry</subject><subject>Fluorometric permeability assay</subject><subject>Immobilized liposome membrane</subject><subject>Lipid Bilayers - chemistry</subject><subject>Liposomes - chemistry</subject><subject>Nanopores</subject><subject>Oxidation-Reduction</subject><subject>Signal Transduction</subject><subject>Sterols - chemistry</subject><subject>Sterols - metabolism</subject><subject>Streptolysin O</subject><subject>Streptolysins - analysis</subject><subject>Streptolysins - chemistry</subject><subject>Streptolysins - metabolism</subject><issn>0731-7085</issn><issn>1873-264X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEuLFDEUhYMoTjv6B1xIlm6qzasqVeBGhvEBI7OZhbuQx60xTSopk5TYS_-56enRpXDh3pDzHTgHodeU7Cmhw7vD_rAavWft3pM2ZHqCdnSUvGOD-PYU7YjktJNk7C_Qi1IOhJCeTuI5umCSD0z0cod-f03R15R9vMdpxvZ7ClAq5BRw-uWdrj5F7CPWOPjVO2x80EfIeIHFZB0Bb-WElpphrSkc2wvfYl0aUCA-_OnocPH3UQdcG1LcZh9cIcACsb5Ez2YdCrx63Jfo7uP13dXn7ub205erDzedFYzVTlPDBZkFM6MjsxxnLfvR9s45ZqVgbuoHQx2X3M2DBM2c5pwZR7Vwk-lHfonenm3XnH5sLaNafLEQQguRtqLoSCYi5MBFk7Kz1OZUSoZZrdkvOh8VJerUvDqoU_Pq1LwibcjUoDeP_ptZwP1D_lbdBO_PAmghf3rIqlgP0YLzGWxVLvn_-f8BKyOYCA</recordid><startdate>20160905</startdate><enddate>20160905</enddate><creator>Shoji, Atsushi</creator><creator>Ikeya, Kana</creator><creator>Aoyagi, Miki</creator><creator>Takatsuji, Ryutaro</creator><creator>Yanagida, Akio</creator><creator>Shibusawa, Yoichi</creator><creator>Sugawara, Masao</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20160905</creationdate><title>Monitoring of cholesterol oxidation in a lipid bilayer membrane using streptolysin O as a sensing and signal transduction element</title><author>Shoji, Atsushi ; Ikeya, Kana ; Aoyagi, Miki ; Takatsuji, Ryutaro ; Yanagida, Akio ; Shibusawa, Yoichi ; Sugawara, Masao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-a1b340f42b8d0f78fa758c5ddd2c742d956b1d373df67ea2da332bd1a4d9b583</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Bacterial Proteins - analysis</topic><topic>Bacterial Proteins - chemistry</topic><topic>Bacterial Proteins - metabolism</topic><topic>Cholesterol</topic><topic>Cholesterol - analysis</topic><topic>Cholesterol - metabolism</topic><topic>Cholesterol oxidase</topic><topic>Cholesterol Oxidase - metabolism</topic><topic>Fluoresceins - chemistry</topic><topic>Fluorometric permeability assay</topic><topic>Immobilized liposome membrane</topic><topic>Lipid Bilayers - chemistry</topic><topic>Liposomes - chemistry</topic><topic>Nanopores</topic><topic>Oxidation-Reduction</topic><topic>Signal Transduction</topic><topic>Sterols - chemistry</topic><topic>Sterols - metabolism</topic><topic>Streptolysin O</topic><topic>Streptolysins - analysis</topic><topic>Streptolysins - chemistry</topic><topic>Streptolysins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shoji, Atsushi</creatorcontrib><creatorcontrib>Ikeya, Kana</creatorcontrib><creatorcontrib>Aoyagi, Miki</creatorcontrib><creatorcontrib>Takatsuji, Ryutaro</creatorcontrib><creatorcontrib>Yanagida, Akio</creatorcontrib><creatorcontrib>Shibusawa, Yoichi</creatorcontrib><creatorcontrib>Sugawara, Masao</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shoji, Atsushi</au><au>Ikeya, Kana</au><au>Aoyagi, Miki</au><au>Takatsuji, Ryutaro</au><au>Yanagida, Akio</au><au>Shibusawa, Yoichi</au><au>Sugawara, Masao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Monitoring of cholesterol oxidation in a lipid bilayer membrane using streptolysin O as a sensing and signal transduction element</atitle><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle><addtitle>J Pharm Biomed Anal</addtitle><date>2016-09-05</date><risdate>2016</risdate><volume>128</volume><spage>455</spage><epage>461</epage><pages>455-461</pages><issn>0731-7085</issn><eissn>1873-264X</eissn><abstract>[Display omitted]
•A fluorometric method for monitoring chol oxidation in a lipid bilayer is described based on the pore forming ability of SLO.•The 3β-OH configuration of sterol in lipid bilayers is important for SLO to form a nanopore.•The P30min value decreased almost linearly with the mole fraction of 4-cholesten-3-one in immobilized liposomes.•The progress of enzymatic chol oxidation in liposomes was detected by utilizing the SLO.
Streptolysin O (SLO), which recognizes sterols and forms nanopores in lipid membranes, is proposed as a sensing element for monitoring cholesterol oxidation in a lipid bilayer. The structural requirements of eight sterols for forming nanopores by SLO confirmed that a free 3-OH group in the β-configuration of sterols is required for recognition by SLO in a lipid bilayer. The extent of nanopore formation by SLO in lipid bilayers increased in the order of cholestanol<cholesterol<25-OH cholesterol and in a sterol concentration-dependent manner. The immobilization of liposomes consisting of PC, cholesterol and 4-cholesten-3-one exhibited a linear relationship between calcein permeability and the molar ratio of cholesterol and 4-cholesten-3-one. The SLO-based method was successfully applied for monitoring of cholesterol oxidase–mediated oxidation of cholesterol in a lipid bilayer. The potential of the SLO nanopore-based method for monitoring cholesterol oxidation in a lipid bilayer by other oxidative enzymes is also discussed.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>27362457</pmid><doi>10.1016/j.jpba.2016.06.009</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0731-7085 |
| ispartof | Journal of pharmaceutical and biomedical analysis, 2016-09, Vol.128, p.455-461 |
| issn | 0731-7085 1873-264X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1809047634 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Bacterial Proteins - analysis Bacterial Proteins - chemistry Bacterial Proteins - metabolism Cholesterol Cholesterol - analysis Cholesterol - metabolism Cholesterol oxidase Cholesterol Oxidase - metabolism Fluoresceins - chemistry Fluorometric permeability assay Immobilized liposome membrane Lipid Bilayers - chemistry Liposomes - chemistry Nanopores Oxidation-Reduction Signal Transduction Sterols - chemistry Sterols - metabolism Streptolysin O Streptolysins - analysis Streptolysins - chemistry Streptolysins - metabolism |
| title | Monitoring of cholesterol oxidation in a lipid bilayer membrane using streptolysin O as a sensing and signal transduction element |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-02T23%3A00%3A31IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Monitoring%20of%20cholesterol%20oxidation%20in%20a%20lipid%20bilayer%20membrane%20using%20streptolysin%20O%20as%20a%20sensing%20and%20signal%20transduction%20element&rft.jtitle=Journal%20of%20pharmaceutical%20and%20biomedical%20analysis&rft.au=Shoji,%20Atsushi&rft.date=2016-09-05&rft.volume=128&rft.spage=455&rft.epage=461&rft.pages=455-461&rft.issn=0731-7085&rft.eissn=1873-264X&rft_id=info:doi/10.1016/j.jpba.2016.06.009&rft_dat=%3Cproquest_cross%3E1809047634%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1809047634&rft_id=info:pmid/27362457&rft_els_id=S0731708516303260&rfr_iscdi=true |