Functional Analysis of CP2-Like Domain and SAM-Like Domain in TFCP2L1, Novel Pluripotency Factor of Embryonic Stem Cells
TFCP2L1 is a transcription factor that facilitates establishment and maintenance of pluripotency in embryonic stem cells by forming a complex transcriptional network with other transcription factors (OCT4, SOX2, and NANOG). TFCP2L1 contains two distinct domains, the CP2-like domain at the N-terminus...
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| Veröffentlicht in: | Applied biochemistry and biotechnology 2016-06, Vol.179 (4), p.650-658 |
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| creator | Kim, Chang Min Jang, Tae-ho Park, Hyun Ho |
| description | TFCP2L1 is a transcription factor that facilitates establishment and maintenance of pluripotency in embryonic stem cells by forming a complex transcriptional network with other transcription factors (OCT4, SOX2, and NANOG). TFCP2L1 contains two distinct domains, the CP2-like domain at the N-terminus and the SAM-like domain at the C-terminus. In this study, we found that TFCP2L1 is hexamerized in solution via the C-terminal SAM-like domain. We also found that homo-oligomerization of SAM-like domain is dependent on the concentration of the proteins. Finally, we found that TFCP2L1 binds directly to DNA via the N-terminal CP2-like domain. |
| doi_str_mv | 10.1007/s12010-016-2021-z |
| format | Article |
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TFCP2L1 contains two distinct domains, the CP2-like domain at the N-terminus and the SAM-like domain at the C-terminus. In this study, we found that TFCP2L1 is hexamerized in solution via the C-terminal SAM-like domain. We also found that homo-oligomerization of SAM-like domain is dependent on the concentration of the proteins. 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genetics</subject><subject>Repressor Proteins - metabolism</subject><subject>SOXB1 Transcription Factors - genetics</subject><subject>SOXB1 Transcription Factors - metabolism</subject><subject>Stem cells</subject><subject>Transcription factors</subject><issn>0273-2289</issn><issn>1559-0291</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkVFLHDEUhUNR6mr7A_oiAV_60GhusjOZPC6rawurFbTPIZO5K2NnJmsyU7r-erOMFSuIEBJIvntyzz2EfAF-DJyrkwiCA2cccia4APbwgUwgyzTjQsMOmXChJBOi0HtkP8Y7zkEUmfpI9kSueQ5QTMjfxdC5vvadbegsbZtYR-pXdH4l2LL-jfTUt7buqO0qej27-O8urZtFApfwjV76P9jQq2YI9dr32LkNXVjX-7AVO2vLsPFd7eh1jy2dY9PET2R3ZZuIn5_OA_JrcXYz_86WP89_zGdL5qZT0TNZulxaviqVkslnViUHTsopIqCsKqWEqGyF00JXWBZcZNKVrpRZmevMARbygHwdddfB3w8Ye9PW0aUObId-iAYKXiiZKy3eR5XWabq5hoQevULv_BDS_EZKpi5lnigYKRd8jAFXZh3q1oaNAW62CZoxQZOcmW2C5iHVHD4pD2WL1XPFv8gSIEYgpqfuFsOLr99UfQRHS6P6</recordid><startdate>20160601</startdate><enddate>20160601</enddate><creator>Kim, Chang Min</creator><creator>Jang, Tae-ho</creator><creator>Park, Hyun Ho</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope><scope>7QO</scope></search><sort><creationdate>20160601</creationdate><title>Functional Analysis of CP2-Like Domain and SAM-Like Domain in TFCP2L1, Novel Pluripotency Factor of Embryonic Stem Cells</title><author>Kim, Chang Min ; Jang, Tae-ho ; Park, Hyun Ho</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c442t-3bc63a0fb7730165d285c334ee1e3dd7722dade489deb80253cbcb35b695c1e83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Biochemistry</topic><topic>Biotechnology</topic><topic>Cell Differentiation - 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| subjects | Animals Biochemistry Biotechnology Cell Differentiation - genetics Chemistry Chemistry and Materials Science DNA-Binding Proteins - chemistry DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism Embryonic Stem Cells - chemistry Embryonic Stem Cells - metabolism Embryos Mice Nanog Homeobox Protein - genetics Nanog Homeobox Protein - metabolism Octamer Transcription Factor-3 - genetics Octamer Transcription Factor-3 - metabolism Protein Domains - genetics Protein Multimerization Proteins Repressor Proteins - chemistry Repressor Proteins - genetics Repressor Proteins - metabolism SOXB1 Transcription Factors - genetics SOXB1 Transcription Factors - metabolism Stem cells Transcription factors |
| title | Functional Analysis of CP2-Like Domain and SAM-Like Domain in TFCP2L1, Novel Pluripotency Factor of Embryonic Stem Cells |
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