Oxytocin Increases Neurite Length and Expression of Cytoskeletal Proteins Associated with Neuronal Growth
Neuropeptide oxytocin acts as a growth and differentiation factor; however, its effects on neurite growth are poorly understood. The aims of the present study were (1) to evaluate time effects of oxytocin on expression of nestin and MAP2; (2) to measure the effect of oxytocin on gene expression of β...
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| Veröffentlicht in: | Journal of molecular neuroscience 2016-06, Vol.59 (2), p.184-192 |
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| creator | Lestanova, Z. Bacova, Z. Kiss, A. Havranek, T. Strbak, V. Bakos, J. |
| description | Neuropeptide oxytocin acts as a growth and differentiation factor; however, its effects on neurite growth are poorly understood. The aims of the present study were (1) to evaluate time effects of oxytocin on expression of nestin and MAP2; (2) to measure the effect of oxytocin on gene expression of β-actin, vimentin, cofilin, and drebrin; and (3) to measure changes in neurite length and number in response to oxytocin/oxytocin receptor antagonist L-371,257. Exposure of SH-SY5Y cells to 1 μM oxytocin resulted in a significant increase in gene expression and protein levels of nestin after 12, 24, and 48 h. Oxytocin treatment induced no changes in gene expression of MAP2; however, a decrease of protein levels was observed in all time intervals. Gene expression of β-actin, vimentin, and drebrin increased in response to oxytocin. Oxytocin induced significant elongation of neurites after 12, 24, and 48 h. No change in neurite length was observed in the presence of the combination of retinoic acid and oxytocin receptor antagonist L-371,257. Oxytocin treatment for 12 h increased the number of neurites. Overall, the present data suggest that oxytocin contributes to the regulation of expression of cytoskeletal proteins associated with growth of neuronal cones and induces neurite elongation mediated by oxytocin receptors at least in certain types of neuronal cells. |
| doi_str_mv | 10.1007/s12031-015-0664-9 |
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The aims of the present study were (1) to evaluate time effects of oxytocin on expression of nestin and MAP2; (2) to measure the effect of oxytocin on gene expression of β-actin, vimentin, cofilin, and drebrin; and (3) to measure changes in neurite length and number in response to oxytocin/oxytocin receptor antagonist L-371,257. Exposure of SH-SY5Y cells to 1 μM oxytocin resulted in a significant increase in gene expression and protein levels of nestin after 12, 24, and 48 h. Oxytocin treatment induced no changes in gene expression of MAP2; however, a decrease of protein levels was observed in all time intervals. Gene expression of β-actin, vimentin, and drebrin increased in response to oxytocin. Oxytocin induced significant elongation of neurites after 12, 24, and 48 h. No change in neurite length was observed in the presence of the combination of retinoic acid and oxytocin receptor antagonist L-371,257. Oxytocin treatment for 12 h increased the number of neurites. 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The aims of the present study were (1) to evaluate time effects of oxytocin on expression of nestin and MAP2; (2) to measure the effect of oxytocin on gene expression of β-actin, vimentin, cofilin, and drebrin; and (3) to measure changes in neurite length and number in response to oxytocin/oxytocin receptor antagonist L-371,257. Exposure of SH-SY5Y cells to 1 μM oxytocin resulted in a significant increase in gene expression and protein levels of nestin after 12, 24, and 48 h. Oxytocin treatment induced no changes in gene expression of MAP2; however, a decrease of protein levels was observed in all time intervals. Gene expression of β-actin, vimentin, and drebrin increased in response to oxytocin. Oxytocin induced significant elongation of neurites after 12, 24, and 48 h. No change in neurite length was observed in the presence of the combination of retinoic acid and oxytocin receptor antagonist L-371,257. Oxytocin treatment for 12 h increased the number of neurites. Overall, the present data suggest that oxytocin contributes to the regulation of expression of cytoskeletal proteins associated with growth of neuronal cones and induces neurite elongation mediated by oxytocin receptors at least in certain types of neuronal cells.</description><subject>Actin Depolymerizing Factors - genetics</subject><subject>Actin Depolymerizing Factors - metabolism</subject><subject>Actins - genetics</subject><subject>Actins - metabolism</subject><subject>Benzoxazines - pharmacology</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cell Biology</subject><subject>Cell Line, Tumor</subject><subject>Cytoskeleton - drug effects</subject><subject>Cytoskeleton - metabolism</subject><subject>Gene expression</subject><subject>Growth Cones - drug effects</subject><subject>Growth Cones - metabolism</subject><subject>Hormones</subject><subject>Humans</subject><subject>Kinases</subject><subject>Microtubule-Associated Proteins - genetics</subject><subject>Microtubule-Associated Proteins - 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Academic</collection><jtitle>Journal of molecular neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lestanova, Z.</au><au>Bacova, Z.</au><au>Kiss, A.</au><au>Havranek, T.</au><au>Strbak, V.</au><au>Bakos, J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Oxytocin Increases Neurite Length and Expression of Cytoskeletal Proteins Associated with Neuronal Growth</atitle><jtitle>Journal of molecular neuroscience</jtitle><stitle>J Mol Neurosci</stitle><addtitle>J Mol Neurosci</addtitle><date>2016-06-01</date><risdate>2016</risdate><volume>59</volume><issue>2</issue><spage>184</spage><epage>192</epage><pages>184-192</pages><issn>0895-8696</issn><eissn>1559-1166</eissn><abstract>Neuropeptide oxytocin acts as a growth and differentiation factor; however, its effects on neurite growth are poorly understood. The aims of the present study were (1) to evaluate time effects of oxytocin on expression of nestin and MAP2; (2) to measure the effect of oxytocin on gene expression of β-actin, vimentin, cofilin, and drebrin; and (3) to measure changes in neurite length and number in response to oxytocin/oxytocin receptor antagonist L-371,257. Exposure of SH-SY5Y cells to 1 μM oxytocin resulted in a significant increase in gene expression and protein levels of nestin after 12, 24, and 48 h. Oxytocin treatment induced no changes in gene expression of MAP2; however, a decrease of protein levels was observed in all time intervals. Gene expression of β-actin, vimentin, and drebrin increased in response to oxytocin. Oxytocin induced significant elongation of neurites after 12, 24, and 48 h. No change in neurite length was observed in the presence of the combination of retinoic acid and oxytocin receptor antagonist L-371,257. Oxytocin treatment for 12 h increased the number of neurites. Overall, the present data suggest that oxytocin contributes to the regulation of expression of cytoskeletal proteins associated with growth of neuronal cones and induces neurite elongation mediated by oxytocin receptors at least in certain types of neuronal cells.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>26474566</pmid><doi>10.1007/s12031-015-0664-9</doi><tpages>9</tpages></addata></record> |
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| subjects | Actin Depolymerizing Factors - genetics Actin Depolymerizing Factors - metabolism Actins - genetics Actins - metabolism Benzoxazines - pharmacology Biomedical and Life Sciences Biomedicine Cell Biology Cell Line, Tumor Cytoskeleton - drug effects Cytoskeleton - metabolism Gene expression Growth Cones - drug effects Growth Cones - metabolism Hormones Humans Kinases Microtubule-Associated Proteins - genetics Microtubule-Associated Proteins - metabolism Nestin - genetics Nestin - metabolism Neuroblastoma Neurochemistry Neurology Neurons Neuropeptides Neuropeptides - genetics Neuropeptides - metabolism Neurosciences Oxytocics - pharmacology Oxytocin - pharmacology Physiology Piperidines - pharmacology Proteins Proteomics Vimentin - genetics Vimentin - metabolism |
| title | Oxytocin Increases Neurite Length and Expression of Cytoskeletal Proteins Associated with Neuronal Growth |
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