third vegetative generation of a field-grown transgenic pineapple clone shows minor side effects of transformation on plant physiological parameters
Genetic modifications of pineapple provide the potential to increase fungal resistance without the use of pesticides. Previously, we developed a procedure for pineapple callus transformation, introducing the antifungal chitinase and AP24 genes, and the herbicide-tolerance conferring bar gene as a se...
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| Veröffentlicht in: | Plant cell, tissue and organ culture tissue and organ culture, 2016-05, Vol.125 (2), p.303-308 |
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| creator | Yabor, Lourdes Bárbara Valle René Carlos RodrÃguez Carlos Aragón Jutta Papenbrock Christoph C. Tebbe José Carlos Lorenzo |
| description | Genetic modifications of pineapple provide the potential to increase fungal resistance without the use of pesticides. Previously, we developed a procedure for pineapple callus transformation, introducing the antifungal chitinase and AP24 genes, and the herbicide-tolerance conferring bar gene as a selectable marker. The three recombinant genes were under the control of the constitutively expressing promoters OCS-35S CaMV-rice actin I, 35S CaMV, and maize Ubi1, respectively. The objective of this study was to analyze whether these genetic modifications caused unintentional side effects on growth-related properties under field conditions. We analyzed the third vegetative generation of a transgenic pineapple clone, approximately 7Â years after the genetic modification occurred. Plant height and diameter, chlorophyll and phenolic contents, fruit mass and D leaf (middle-age) mass, water content, succulence, sap pH, transpiration, COâ assimilation, and water use efficiency were recorded and compared to a micro-propagated cultivar. A macro-propagated variant was also cultivated at the field site in order to distinguish unintended effects of the propagation method from that of the genetic modification. While all parameters analyzed in this study were highly similar to each other for the three cultivars, statistically analyses revealed significant deviations of the genetically modified variant for the succulence index being higher at in the morning and evening, carbon dioxide assimilation being lower at noon and, at night, the water use efficiency was lower. The minor deviations were all within the range which can be expected between common cultivars grown together. |
| doi_str_mv | 10.1007/s11240-016-0950-4 |
| format | Article |
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Tebbe ; José Carlos Lorenzo</creator><creatorcontrib>Yabor, Lourdes ; Bárbara Valle ; René Carlos RodrÃguez ; Carlos Aragón ; Jutta Papenbrock ; Christoph C. Tebbe ; José Carlos Lorenzo</creatorcontrib><description>Genetic modifications of pineapple provide the potential to increase fungal resistance without the use of pesticides. Previously, we developed a procedure for pineapple callus transformation, introducing the antifungal chitinase and AP24 genes, and the herbicide-tolerance conferring bar gene as a selectable marker. The three recombinant genes were under the control of the constitutively expressing promoters OCS-35S CaMV-rice actin I, 35S CaMV, and maize Ubi1, respectively. The objective of this study was to analyze whether these genetic modifications caused unintentional side effects on growth-related properties under field conditions. We analyzed the third vegetative generation of a transgenic pineapple clone, approximately 7 years after the genetic modification occurred. Plant height and diameter, chlorophyll and phenolic contents, fruit mass and D leaf (middle-age) mass, water content, succulence, sap pH, transpiration, COâ assimilation, and water use efficiency were recorded and compared to a micro-propagated cultivar. A macro-propagated variant was also cultivated at the field site in order to distinguish unintended effects of the propagation method from that of the genetic modification. While all parameters analyzed in this study were highly similar to each other for the three cultivars, statistically analyses revealed significant deviations of the genetically modified variant for the succulence index being higher at in the morning and evening, carbon dioxide assimilation being lower at noon and, at night, the water use efficiency was lower. The minor deviations were all within the range which can be expected between common cultivars grown together.</description><identifier>ISSN: 0167-6857</identifier><identifier>EISSN: 1573-5044</identifier><identifier>DOI: 10.1007/s11240-016-0950-4</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Actin ; adverse effects ; Assimilation ; Bar gene ; Biomedical and Life Sciences ; Callus ; Carbon dioxide ; Chitinase ; Chlorophyll ; Cloning ; corn ; Cultivars ; Fruits ; fungi ; Fungicides ; Genes ; genetic engineering ; Genetic modification ; Herbicides ; leaves ; Life Sciences ; Moisture content ; Original Article ; Parameter modification ; Pesticides ; Phenolic compounds ; Phenols ; Physiological effects ; Pineapples ; Plant Genetics and Genomics ; Plant Pathology ; Plant Physiology ; Plant Sciences ; Propagation ; sap ; Side effects ; Statistical analysis ; Transgenic plants ; Transpiration ; Water content ; Water use ; Water use efficiency ; Zea mays</subject><ispartof>Plant cell, tissue and organ culture, 2016-05, Vol.125 (2), p.303-308</ispartof><rights>Springer Science+Business Media Dordrecht 2016</rights><rights>Plant Cell, Tissue and Organ Culture (PCTOC) is a copyright of Springer, (2016). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c373t-3edc5821293ad4c49eb36952b59f9803cad1ca2849e0998c8c0435c09e597e243</citedby><cites>FETCH-LOGICAL-c373t-3edc5821293ad4c49eb36952b59f9803cad1ca2849e0998c8c0435c09e597e243</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11240-016-0950-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11240-016-0950-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Yabor, Lourdes</creatorcontrib><creatorcontrib>Bárbara Valle</creatorcontrib><creatorcontrib>René Carlos RodrÃguez</creatorcontrib><creatorcontrib>Carlos Aragón</creatorcontrib><creatorcontrib>Jutta Papenbrock</creatorcontrib><creatorcontrib>Christoph C. Tebbe</creatorcontrib><creatorcontrib>José Carlos Lorenzo</creatorcontrib><title>third vegetative generation of a field-grown transgenic pineapple clone shows minor side effects of transformation on plant physiological parameters</title><title>Plant cell, tissue and organ culture</title><addtitle>Plant Cell Tiss Organ Cult</addtitle><description>Genetic modifications of pineapple provide the potential to increase fungal resistance without the use of pesticides. Previously, we developed a procedure for pineapple callus transformation, introducing the antifungal chitinase and AP24 genes, and the herbicide-tolerance conferring bar gene as a selectable marker. The three recombinant genes were under the control of the constitutively expressing promoters OCS-35S CaMV-rice actin I, 35S CaMV, and maize Ubi1, respectively. The objective of this study was to analyze whether these genetic modifications caused unintentional side effects on growth-related properties under field conditions. We analyzed the third vegetative generation of a transgenic pineapple clone, approximately 7 years after the genetic modification occurred. Plant height and diameter, chlorophyll and phenolic contents, fruit mass and D leaf (middle-age) mass, water content, succulence, sap pH, transpiration, COâ assimilation, and water use efficiency were recorded and compared to a micro-propagated cultivar. A macro-propagated variant was also cultivated at the field site in order to distinguish unintended effects of the propagation method from that of the genetic modification. While all parameters analyzed in this study were highly similar to each other for the three cultivars, statistically analyses revealed significant deviations of the genetically modified variant for the succulence index being higher at in the morning and evening, carbon dioxide assimilation being lower at noon and, at night, the water use efficiency was lower. The minor deviations were all within the range which can be expected between common cultivars grown together.</description><subject>Actin</subject><subject>adverse effects</subject><subject>Assimilation</subject><subject>Bar gene</subject><subject>Biomedical and Life Sciences</subject><subject>Callus</subject><subject>Carbon dioxide</subject><subject>Chitinase</subject><subject>Chlorophyll</subject><subject>Cloning</subject><subject>corn</subject><subject>Cultivars</subject><subject>Fruits</subject><subject>fungi</subject><subject>Fungicides</subject><subject>Genes</subject><subject>genetic engineering</subject><subject>Genetic modification</subject><subject>Herbicides</subject><subject>leaves</subject><subject>Life Sciences</subject><subject>Moisture content</subject><subject>Original Article</subject><subject>Parameter modification</subject><subject>Pesticides</subject><subject>Phenolic compounds</subject><subject>Phenols</subject><subject>Physiological effects</subject><subject>Pineapples</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Pathology</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Propagation</subject><subject>sap</subject><subject>Side effects</subject><subject>Statistical analysis</subject><subject>Transgenic plants</subject><subject>Transpiration</subject><subject>Water content</subject><subject>Water use</subject><subject>Water use efficiency</subject><subject>Zea mays</subject><issn>0167-6857</issn><issn>1573-5044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kc1q3TAQhUVpoLdJHqCrCrrpxunoz5aWJTRtIdBFk7VQ5LGvgi25km9C3qMPXN06UMiii2EG5juHA4eQdwwuGED3qTDGJTTA2gaMgka-IjumOtEokPI12dVH17RadW_I21LuAaAVku3I73Ufck8fcMTVreEB6YgRcz1TpGmgjg4Bp74Zc3qMdM0ulgoET5cQ0S3LhNRPKSIt-_RY6BxiyrSEHikOA_q1HE3-yoaU52fbSJfJxZUu-6cS0pTG4N1EF5fdjCvmckZOBjcVPH_ep-T26svN5bfm-sfX75efrxsvOrE2AnuvNGfcCNdLLw3eidYofqfMYDQI73rmHdf1AcZorz1IoTwYVKZDLsUp-bj5Ljn9OmBZ7RyKx6mGw3QolmnQHZN1KvrhBXqfDjnWdJZzZYTuNNOVYhvlcyol42CXHGaXnywDe-zJbj3ZWoc99mSPIfimKZWNI-Z_zv8Tvd9Eg0vWjTkUe_uTVwCA6dYYLv4AXyChfw</recordid><startdate>20160501</startdate><enddate>20160501</enddate><creator>Yabor, Lourdes</creator><creator>Bárbara Valle</creator><creator>René Carlos RodrÃguez</creator><creator>Carlos Aragón</creator><creator>Jutta Papenbrock</creator><creator>Christoph C. Tebbe</creator><creator>José Carlos Lorenzo</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20160501</creationdate><title>third vegetative generation of a field-grown transgenic pineapple clone shows minor side effects of transformation on plant physiological parameters</title><author>Yabor, Lourdes ; Bárbara Valle ; René Carlos RodrÃguez ; Carlos Aragón ; Jutta Papenbrock ; Christoph C. Tebbe ; José Carlos Lorenzo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c373t-3edc5821293ad4c49eb36952b59f9803cad1ca2849e0998c8c0435c09e597e243</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Actin</topic><topic>adverse effects</topic><topic>Assimilation</topic><topic>Bar gene</topic><topic>Biomedical and Life Sciences</topic><topic>Callus</topic><topic>Carbon dioxide</topic><topic>Chitinase</topic><topic>Chlorophyll</topic><topic>Cloning</topic><topic>corn</topic><topic>Cultivars</topic><topic>Fruits</topic><topic>fungi</topic><topic>Fungicides</topic><topic>Genes</topic><topic>genetic engineering</topic><topic>Genetic modification</topic><topic>Herbicides</topic><topic>leaves</topic><topic>Life Sciences</topic><topic>Moisture content</topic><topic>Original Article</topic><topic>Parameter modification</topic><topic>Pesticides</topic><topic>Phenolic compounds</topic><topic>Phenols</topic><topic>Physiological effects</topic><topic>Pineapples</topic><topic>Plant Genetics and Genomics</topic><topic>Plant Pathology</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Propagation</topic><topic>sap</topic><topic>Side effects</topic><topic>Statistical analysis</topic><topic>Transgenic plants</topic><topic>Transpiration</topic><topic>Water content</topic><topic>Water use</topic><topic>Water use efficiency</topic><topic>Zea mays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yabor, Lourdes</creatorcontrib><creatorcontrib>Bárbara Valle</creatorcontrib><creatorcontrib>René Carlos RodrÃguez</creatorcontrib><creatorcontrib>Carlos Aragón</creatorcontrib><creatorcontrib>Jutta Papenbrock</creatorcontrib><creatorcontrib>Christoph C. 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Tebbe</au><au>José Carlos Lorenzo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>third vegetative generation of a field-grown transgenic pineapple clone shows minor side effects of transformation on plant physiological parameters</atitle><jtitle>Plant cell, tissue and organ culture</jtitle><stitle>Plant Cell Tiss Organ Cult</stitle><date>2016-05-01</date><risdate>2016</risdate><volume>125</volume><issue>2</issue><spage>303</spage><epage>308</epage><pages>303-308</pages><issn>0167-6857</issn><eissn>1573-5044</eissn><abstract>Genetic modifications of pineapple provide the potential to increase fungal resistance without the use of pesticides. Previously, we developed a procedure for pineapple callus transformation, introducing the antifungal chitinase and AP24 genes, and the herbicide-tolerance conferring bar gene as a selectable marker. The three recombinant genes were under the control of the constitutively expressing promoters OCS-35S CaMV-rice actin I, 35S CaMV, and maize Ubi1, respectively. The objective of this study was to analyze whether these genetic modifications caused unintentional side effects on growth-related properties under field conditions. We analyzed the third vegetative generation of a transgenic pineapple clone, approximately 7 years after the genetic modification occurred. Plant height and diameter, chlorophyll and phenolic contents, fruit mass and D leaf (middle-age) mass, water content, succulence, sap pH, transpiration, COâ assimilation, and water use efficiency were recorded and compared to a micro-propagated cultivar. A macro-propagated variant was also cultivated at the field site in order to distinguish unintended effects of the propagation method from that of the genetic modification. While all parameters analyzed in this study were highly similar to each other for the three cultivars, statistically analyses revealed significant deviations of the genetically modified variant for the succulence index being higher at in the morning and evening, carbon dioxide assimilation being lower at noon and, at night, the water use efficiency was lower. The minor deviations were all within the range which can be expected between common cultivars grown together.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s11240-016-0950-4</doi><tpages>6</tpages></addata></record> |
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| ispartof | Plant cell, tissue and organ culture, 2016-05, Vol.125 (2), p.303-308 |
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| source | Springer Nature - Complete Springer Journals |
| subjects | Actin adverse effects Assimilation Bar gene Biomedical and Life Sciences Callus Carbon dioxide Chitinase Chlorophyll Cloning corn Cultivars Fruits fungi Fungicides Genes genetic engineering Genetic modification Herbicides leaves Life Sciences Moisture content Original Article Parameter modification Pesticides Phenolic compounds Phenols Physiological effects Pineapples Plant Genetics and Genomics Plant Pathology Plant Physiology Plant Sciences Propagation sap Side effects Statistical analysis Transgenic plants Transpiration Water content Water use Water use efficiency Zea mays |
| title | third vegetative generation of a field-grown transgenic pineapple clone shows minor side effects of transformation on plant physiological parameters |
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