Identification and characterization of profilin antigen among Babesia species as a common vaccine candidate against babesiosis
We have characterized a member of the profilin (PROF) family protein as a common antigen in three pathogens—Babesia bovis (B. bovis), Babesia bigemina (B. bigemina), and Babesia microti (B. microti)—and evaluated its immunogenic and cross-protective properties against a challenge infection with B. m...
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| Veröffentlicht in: | Experimental parasitology 2016-07, Vol.166, p.29-36 |
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| creator | Munkhjargal, Tserendorj Aboge, Gabriel Oluga Ueno, Akio Aboulaila, Mahmoud Yokoyama, Naoaki Igarashi, Ikuo |
| description | We have characterized a member of the profilin (PROF) family protein as a common antigen in three pathogens—Babesia bovis (B. bovis), Babesia bigemina (B. bigemina), and Babesia microti (B. microti)—and evaluated its immunogenic and cross-protective properties against a challenge infection with B. microti in BALB/c mice. The recombinant PROF proteins of B. bovis, B. bigemina, and B. microti were successfully expressed in Escherichia coli (E. coli) as soluble GST fusion proteins (rBboPROF, rBbigPROF, and rBmPROF, respectively), and they were found to be antigenic. On probing with mouse anti-rPROF serum, green fluorescence was observed on the parasites’ cytosols by confocal laser microscopy. Immunization regimes in BALB/c mice using rPROFs induced cross-protective immunity against B. microti infection based on high levels of cytokines and immunoglobulin (IgG) titers, a reduction in peak parasitemia levels, and earlier clearance of the parasite as compared with control mice. The findings of the present study indicate that PROF is a common antigen among bovine and murine Babesia parasites, and it might be used as a common vaccine candidate against babesiosis.
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•rPROF proteins of Babesia bovis, Babesia bigemina, and Babesia microti were antigenic.•rPROF showed cross-protective effect against B. microti infection.•rPROF induced a high levels of cytokines and immunoglobulin titers.•PROF might be used as a common vaccine candidate against babesiosis. |
| doi_str_mv | 10.1016/j.exppara.2016.03.024 |
| format | Article |
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[Display omitted]
•rPROF proteins of Babesia bovis, Babesia bigemina, and Babesia microti were antigenic.•rPROF showed cross-protective effect against B. microti infection.•rPROF induced a high levels of cytokines and immunoglobulin titers.•PROF might be used as a common vaccine candidate against babesiosis.</description><identifier>ISSN: 0014-4894</identifier><identifier>EISSN: 1090-2449</identifier><identifier>DOI: 10.1016/j.exppara.2016.03.024</identifier><identifier>PMID: 27003460</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Amino Acid Sequence ; Animals ; Antibodies, Protozoan - blood ; Antigens, Protozoan - chemistry ; Antigens, Protozoan - genetics ; Antigens, Protozoan - immunology ; Babesia ; Babesia - classification ; Babesia - genetics ; Babesia - immunology ; Babesia bigemina ; Babesia microti ; Babesiosis - prevention & control ; Base Sequence ; Cattle ; Computational Biology ; Enzyme-Linked Immunosorbent Assay ; Erythrocytes - parasitology ; Escherichia coli ; Female ; Fluorescent Antibody Technique, Indirect ; Immunization ; Immunoglobulin G - blood ; Mice ; Mice, Inbred BALB C ; Microscopy, Confocal ; Phylogeny ; Profilins - chemistry ; Profilins - genetics ; Profilins - immunology ; Protozoa ; Protozoan Vaccines ; Recombinant protein ; RNA, Protozoan - isolation & purification ; Sequence Alignment</subject><ispartof>Experimental parasitology, 2016-07, Vol.166, p.29-36</ispartof><rights>2016 Elsevier Inc.</rights><rights>Copyright © 2016 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c464t-284adac6c944d332921c27b3902e271e3c26a88f2aeb1cdd7cc6a217e17f213c3</citedby><cites>FETCH-LOGICAL-c464t-284adac6c944d332921c27b3902e271e3c26a88f2aeb1cdd7cc6a217e17f213c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.exppara.2016.03.024$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27003460$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Munkhjargal, Tserendorj</creatorcontrib><creatorcontrib>Aboge, Gabriel Oluga</creatorcontrib><creatorcontrib>Ueno, Akio</creatorcontrib><creatorcontrib>Aboulaila, Mahmoud</creatorcontrib><creatorcontrib>Yokoyama, Naoaki</creatorcontrib><creatorcontrib>Igarashi, Ikuo</creatorcontrib><title>Identification and characterization of profilin antigen among Babesia species as a common vaccine candidate against babesiosis</title><title>Experimental parasitology</title><addtitle>Exp Parasitol</addtitle><description>We have characterized a member of the profilin (PROF) family protein as a common antigen in three pathogens—Babesia bovis (B. bovis), Babesia bigemina (B. bigemina), and Babesia microti (B. microti)—and evaluated its immunogenic and cross-protective properties against a challenge infection with B. microti in BALB/c mice. The recombinant PROF proteins of B. bovis, B. bigemina, and B. microti were successfully expressed in Escherichia coli (E. coli) as soluble GST fusion proteins (rBboPROF, rBbigPROF, and rBmPROF, respectively), and they were found to be antigenic. On probing with mouse anti-rPROF serum, green fluorescence was observed on the parasites’ cytosols by confocal laser microscopy. Immunization regimes in BALB/c mice using rPROFs induced cross-protective immunity against B. microti infection based on high levels of cytokines and immunoglobulin (IgG) titers, a reduction in peak parasitemia levels, and earlier clearance of the parasite as compared with control mice. The findings of the present study indicate that PROF is a common antigen among bovine and murine Babesia parasites, and it might be used as a common vaccine candidate against babesiosis.
[Display omitted]
•rPROF proteins of Babesia bovis, Babesia bigemina, and Babesia microti were antigenic.•rPROF showed cross-protective effect against B. microti infection.•rPROF induced a high levels of cytokines and immunoglobulin titers.•PROF might be used as a common vaccine candidate against babesiosis.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antibodies, Protozoan - blood</subject><subject>Antigens, Protozoan - chemistry</subject><subject>Antigens, Protozoan - genetics</subject><subject>Antigens, Protozoan - immunology</subject><subject>Babesia</subject><subject>Babesia - classification</subject><subject>Babesia - genetics</subject><subject>Babesia - immunology</subject><subject>Babesia bigemina</subject><subject>Babesia microti</subject><subject>Babesiosis - prevention & control</subject><subject>Base Sequence</subject><subject>Cattle</subject><subject>Computational Biology</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Erythrocytes - parasitology</subject><subject>Escherichia coli</subject><subject>Female</subject><subject>Fluorescent Antibody Technique, Indirect</subject><subject>Immunization</subject><subject>Immunoglobulin G - blood</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microscopy, Confocal</subject><subject>Phylogeny</subject><subject>Profilins - chemistry</subject><subject>Profilins - genetics</subject><subject>Profilins - immunology</subject><subject>Protozoa</subject><subject>Protozoan Vaccines</subject><subject>Recombinant protein</subject><subject>RNA, Protozoan - isolation & purification</subject><subject>Sequence Alignment</subject><issn>0014-4894</issn><issn>1090-2449</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2LFDEQhoMo7rj6E5QcvXRbSWc7nZPo4sfCghc9h-pK9Zhh-sOkZ3E9-NvNOKPXhUCRyvO-ReoV4qWCWoFq3-xq_rksmLDW5VpDU4M2j8RGgYNKG-Meiw2AMpXpnLkQz3LeAUCntHkqLrQFaEwLG_H7JvC0xiESrnGeJE5B0vdiSyun-OvUnAe5pHmI-3gE1rjlUsd52sr32HOOKPPCFDlLLEfSPJZHeYdEcWJJxTMGXFniFuOUV9n_Vc055ufiyYD7zC_O9VJ8-_jh6_Xn6vbLp5vrd7cVmdasle4MBqSWnDGhabTTirTtGweatVXckG6x6waN3CsKwRK1qJVlZQetGmouxeuTb_nHjwPn1Y8xE-_3OPF8yF510FlonVMPo9bZK9OqThf06oRSmnNOPPglxRHTvVfgjyn5nT-n5I8peWh8SanoXp1HHPqRw3_Vv1gK8PYEcNnJXeTkc1nvRBxiYlp9mOMDI_4AEran6w</recordid><startdate>201607</startdate><enddate>201607</enddate><creator>Munkhjargal, Tserendorj</creator><creator>Aboge, Gabriel Oluga</creator><creator>Ueno, Akio</creator><creator>Aboulaila, Mahmoud</creator><creator>Yokoyama, Naoaki</creator><creator>Igarashi, Ikuo</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T5</scope><scope>H94</scope><scope>M7N</scope></search><sort><creationdate>201607</creationdate><title>Identification and characterization of profilin antigen among Babesia species as a common vaccine candidate against babesiosis</title><author>Munkhjargal, Tserendorj ; Aboge, Gabriel Oluga ; Ueno, Akio ; Aboulaila, Mahmoud ; Yokoyama, Naoaki ; Igarashi, Ikuo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c464t-284adac6c944d332921c27b3902e271e3c26a88f2aeb1cdd7cc6a217e17f213c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Antibodies, Protozoan - blood</topic><topic>Antigens, Protozoan - chemistry</topic><topic>Antigens, Protozoan - genetics</topic><topic>Antigens, Protozoan - immunology</topic><topic>Babesia</topic><topic>Babesia - classification</topic><topic>Babesia - genetics</topic><topic>Babesia - immunology</topic><topic>Babesia bigemina</topic><topic>Babesia microti</topic><topic>Babesiosis - prevention & control</topic><topic>Base Sequence</topic><topic>Cattle</topic><topic>Computational Biology</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Erythrocytes - parasitology</topic><topic>Escherichia coli</topic><topic>Female</topic><topic>Fluorescent Antibody Technique, Indirect</topic><topic>Immunization</topic><topic>Immunoglobulin G - blood</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microscopy, Confocal</topic><topic>Phylogeny</topic><topic>Profilins - chemistry</topic><topic>Profilins - genetics</topic><topic>Profilins - immunology</topic><topic>Protozoa</topic><topic>Protozoan Vaccines</topic><topic>Recombinant protein</topic><topic>RNA, Protozoan - isolation & purification</topic><topic>Sequence Alignment</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Munkhjargal, Tserendorj</creatorcontrib><creatorcontrib>Aboge, Gabriel Oluga</creatorcontrib><creatorcontrib>Ueno, Akio</creatorcontrib><creatorcontrib>Aboulaila, Mahmoud</creatorcontrib><creatorcontrib>Yokoyama, Naoaki</creatorcontrib><creatorcontrib>Igarashi, Ikuo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Experimental parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Munkhjargal, Tserendorj</au><au>Aboge, Gabriel Oluga</au><au>Ueno, Akio</au><au>Aboulaila, Mahmoud</au><au>Yokoyama, Naoaki</au><au>Igarashi, Ikuo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and characterization of profilin antigen among Babesia species as a common vaccine candidate against babesiosis</atitle><jtitle>Experimental parasitology</jtitle><addtitle>Exp Parasitol</addtitle><date>2016-07</date><risdate>2016</risdate><volume>166</volume><spage>29</spage><epage>36</epage><pages>29-36</pages><issn>0014-4894</issn><eissn>1090-2449</eissn><abstract>We have characterized a member of the profilin (PROF) family protein as a common antigen in three pathogens—Babesia bovis (B. bovis), Babesia bigemina (B. bigemina), and Babesia microti (B. microti)—and evaluated its immunogenic and cross-protective properties against a challenge infection with B. microti in BALB/c mice. The recombinant PROF proteins of B. bovis, B. bigemina, and B. microti were successfully expressed in Escherichia coli (E. coli) as soluble GST fusion proteins (rBboPROF, rBbigPROF, and rBmPROF, respectively), and they were found to be antigenic. On probing with mouse anti-rPROF serum, green fluorescence was observed on the parasites’ cytosols by confocal laser microscopy. Immunization regimes in BALB/c mice using rPROFs induced cross-protective immunity against B. microti infection based on high levels of cytokines and immunoglobulin (IgG) titers, a reduction in peak parasitemia levels, and earlier clearance of the parasite as compared with control mice. The findings of the present study indicate that PROF is a common antigen among bovine and murine Babesia parasites, and it might be used as a common vaccine candidate against babesiosis.
[Display omitted]
•rPROF proteins of Babesia bovis, Babesia bigemina, and Babesia microti were antigenic.•rPROF showed cross-protective effect against B. microti infection.•rPROF induced a high levels of cytokines and immunoglobulin titers.•PROF might be used as a common vaccine candidate against babesiosis.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>27003460</pmid><doi>10.1016/j.exppara.2016.03.024</doi><tpages>8</tpages></addata></record> |
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| ispartof | Experimental parasitology, 2016-07, Vol.166, p.29-36 |
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| subjects | Amino Acid Sequence Animals Antibodies, Protozoan - blood Antigens, Protozoan - chemistry Antigens, Protozoan - genetics Antigens, Protozoan - immunology Babesia Babesia - classification Babesia - genetics Babesia - immunology Babesia bigemina Babesia microti Babesiosis - prevention & control Base Sequence Cattle Computational Biology Enzyme-Linked Immunosorbent Assay Erythrocytes - parasitology Escherichia coli Female Fluorescent Antibody Technique, Indirect Immunization Immunoglobulin G - blood Mice Mice, Inbred BALB C Microscopy, Confocal Phylogeny Profilins - chemistry Profilins - genetics Profilins - immunology Protozoa Protozoan Vaccines Recombinant protein RNA, Protozoan - isolation & purification Sequence Alignment |
| title | Identification and characterization of profilin antigen among Babesia species as a common vaccine candidate against babesiosis |
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