Tracking inflammation in the epileptic rat brain by bi-functional fluorescent and magnetic nanoparticles
Abstract Correct localization of epileptic foci can improve surgical outcome in patients with drug-resistant seizures. Our aim was to demonstrate that systemically injected nanoparticles identify activated immune cells, which have been reported to accumulate in epileptogenic brain tissue. Fluorescen...
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| Veröffentlicht in: | Nanomedicine 2016-07, Vol.12 (5), p.1335-1345 |
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| creator | Portnoy, Emma Polyak, Boris Inbar, Dorrit Kenan, Gilad Rai, Ahmad Wehrli, Suzanne L Roberts, Timothy P.L Bishara, Ameer Mann, Aniv Shmuel, Miriam Rozovsky, Katya Itzhak, Gal Hur, Tamir Ben Magdassi, Shlomo Ekstein, Dana Eyal, Sara |
| description | Abstract Correct localization of epileptic foci can improve surgical outcome in patients with drug-resistant seizures. Our aim was to demonstrate that systemically injected nanoparticles identify activated immune cells, which have been reported to accumulate in epileptogenic brain tissue. Fluorescent and magnetite-labeled nanoparticles were injected intravenously to rats with lithium-pilocarpine-induced chronic epilepsy. Cerebral uptake was studied ex vivo by confocal microscopy and MRI. Cellular uptake and biological effects were characterized in vitro in murine monocytes and microglia cell lines. Microscopy confirmed that the nanoparticles selectively accumulate within myeloid cells in the hippocampus, in association with inflammation. The nanoparticle signal was also detectable by MRI. The in vitro studies demonstrate rapid nanoparticle uptake and good cellular tolerability. We show that nanoparticles can target myeloid cells in epileptogenic brain tissue. This system can contribute to pre-surgical and intra-surgical localization of epileptic foci, and assist in detecting immune system involvement in epilepsy. |
| doi_str_mv | 10.1016/j.nano.2016.01.018 |
| format | Article |
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Our aim was to demonstrate that systemically injected nanoparticles identify activated immune cells, which have been reported to accumulate in epileptogenic brain tissue. Fluorescent and magnetite-labeled nanoparticles were injected intravenously to rats with lithium-pilocarpine-induced chronic epilepsy. Cerebral uptake was studied ex vivo by confocal microscopy and MRI. Cellular uptake and biological effects were characterized in vitro in murine monocytes and microglia cell lines. Microscopy confirmed that the nanoparticles selectively accumulate within myeloid cells in the hippocampus, in association with inflammation. The nanoparticle signal was also detectable by MRI. The in vitro studies demonstrate rapid nanoparticle uptake and good cellular tolerability. We show that nanoparticles can target myeloid cells in epileptogenic brain tissue. This system can contribute to pre-surgical and intra-surgical localization of epileptic foci, and assist in detecting immune system involvement in epilepsy.</description><identifier>ISSN: 1549-9634</identifier><identifier>EISSN: 1549-9642</identifier><identifier>DOI: 10.1016/j.nano.2016.01.018</identifier><identifier>PMID: 26964483</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Brain ; Epilepsy ; Epilepsy - surgery ; Hippocampus ; Humans ; Imaging ; Inflammation ; Internal Medicine ; Magnetic nanoparticles ; Magnetic Resonance Imaging ; Magnetic Resonance Imaging - methods ; Magnetite Nanoparticles ; Mice ; Microscopy, Confocal ; Rats</subject><ispartof>Nanomedicine, 2016-07, Vol.12 (5), p.1335-1345</ispartof><rights>Elsevier Inc.</rights><rights>2016 Elsevier Inc.</rights><rights>Copyright © 2016 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c444t-9c941b023f08e90f4491b0681bdb36045313274cf9d25e7c1daed3b0a02315d63</citedby><cites>FETCH-LOGICAL-c444t-9c941b023f08e90f4491b0681bdb36045313274cf9d25e7c1daed3b0a02315d63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.nano.2016.01.018$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26964483$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Portnoy, Emma</creatorcontrib><creatorcontrib>Polyak, Boris</creatorcontrib><creatorcontrib>Inbar, Dorrit</creatorcontrib><creatorcontrib>Kenan, Gilad</creatorcontrib><creatorcontrib>Rai, Ahmad</creatorcontrib><creatorcontrib>Wehrli, Suzanne L</creatorcontrib><creatorcontrib>Roberts, Timothy P.L</creatorcontrib><creatorcontrib>Bishara, Ameer</creatorcontrib><creatorcontrib>Mann, Aniv</creatorcontrib><creatorcontrib>Shmuel, Miriam</creatorcontrib><creatorcontrib>Rozovsky, Katya</creatorcontrib><creatorcontrib>Itzhak, Gal</creatorcontrib><creatorcontrib>Hur, Tamir Ben</creatorcontrib><creatorcontrib>Magdassi, Shlomo</creatorcontrib><creatorcontrib>Ekstein, Dana</creatorcontrib><creatorcontrib>Eyal, Sara</creatorcontrib><title>Tracking inflammation in the epileptic rat brain by bi-functional fluorescent and magnetic nanoparticles</title><title>Nanomedicine</title><addtitle>Nanomedicine</addtitle><description>Abstract Correct localization of epileptic foci can improve surgical outcome in patients with drug-resistant seizures. Our aim was to demonstrate that systemically injected nanoparticles identify activated immune cells, which have been reported to accumulate in epileptogenic brain tissue. Fluorescent and magnetite-labeled nanoparticles were injected intravenously to rats with lithium-pilocarpine-induced chronic epilepsy. Cerebral uptake was studied ex vivo by confocal microscopy and MRI. Cellular uptake and biological effects were characterized in vitro in murine monocytes and microglia cell lines. Microscopy confirmed that the nanoparticles selectively accumulate within myeloid cells in the hippocampus, in association with inflammation. The nanoparticle signal was also detectable by MRI. The in vitro studies demonstrate rapid nanoparticle uptake and good cellular tolerability. We show that nanoparticles can target myeloid cells in epileptogenic brain tissue. This system can contribute to pre-surgical and intra-surgical localization of epileptic foci, and assist in detecting immune system involvement in epilepsy.</description><subject>Animals</subject><subject>Brain</subject><subject>Epilepsy</subject><subject>Epilepsy - surgery</subject><subject>Hippocampus</subject><subject>Humans</subject><subject>Imaging</subject><subject>Inflammation</subject><subject>Internal Medicine</subject><subject>Magnetic nanoparticles</subject><subject>Magnetic Resonance Imaging</subject><subject>Magnetic Resonance Imaging - methods</subject><subject>Magnetite Nanoparticles</subject><subject>Mice</subject><subject>Microscopy, Confocal</subject><subject>Rats</subject><issn>1549-9634</issn><issn>1549-9642</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkstq3TAQhkVpadKkL9BF0bIbn44sWZagFEroDQJZNFkLWR4nOpFlV7ID5-0rc9IsumhgQDPi-4e5EfKOwY4Bkx_3u2jjtKuLvwNWTL0gp6wRutJS1C-ffC5OyJuc9wC8BdCvyUktCyEUPyV318m6ex9vqY9DsONoFz_FEtDlDinOPuC8eEeTXWiXbPnvDrTz1bBGt5E20CGsU8LsMC7Uxp6O9jbiptmqm20qbsB8Tl4NNmR8-_iekZtvX68vflSXV99_Xny5rJwQYqm004J1UPMBFGoYhNAllIp1fccliIYzXrfCDbqvG2wd6y32vANbJKzpJT8jH4555zT9XjEvZvSlthBsxGnNhilQUmup-PNoq3kj21q3Ba2PqEtTzgkHMyc_2nQwDMy2DLM3W7tmW4YBVkwV0fvH_Gs3Yv8k-Tv9Anw6AlgG8uAxmew8Roe9T-gW00_-__k__yN3wUfvbLjHA-b9tKayn9KHybUB82s7h-0amAQApRr-B337sFQ</recordid><startdate>20160701</startdate><enddate>20160701</enddate><creator>Portnoy, Emma</creator><creator>Polyak, Boris</creator><creator>Inbar, Dorrit</creator><creator>Kenan, Gilad</creator><creator>Rai, Ahmad</creator><creator>Wehrli, Suzanne L</creator><creator>Roberts, Timothy P.L</creator><creator>Bishara, Ameer</creator><creator>Mann, Aniv</creator><creator>Shmuel, Miriam</creator><creator>Rozovsky, Katya</creator><creator>Itzhak, Gal</creator><creator>Hur, Tamir Ben</creator><creator>Magdassi, Shlomo</creator><creator>Ekstein, Dana</creator><creator>Eyal, Sara</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20160701</creationdate><title>Tracking inflammation in the epileptic rat brain by bi-functional fluorescent and magnetic nanoparticles</title><author>Portnoy, Emma ; Polyak, Boris ; Inbar, Dorrit ; Kenan, Gilad ; Rai, Ahmad ; Wehrli, Suzanne L ; Roberts, Timothy P.L ; Bishara, Ameer ; Mann, Aniv ; Shmuel, Miriam ; Rozovsky, Katya ; Itzhak, Gal ; Hur, Tamir Ben ; Magdassi, Shlomo ; Ekstein, Dana ; Eyal, Sara</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c444t-9c941b023f08e90f4491b0681bdb36045313274cf9d25e7c1daed3b0a02315d63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Brain</topic><topic>Epilepsy</topic><topic>Epilepsy - surgery</topic><topic>Hippocampus</topic><topic>Humans</topic><topic>Imaging</topic><topic>Inflammation</topic><topic>Internal Medicine</topic><topic>Magnetic nanoparticles</topic><topic>Magnetic Resonance Imaging</topic><topic>Magnetic Resonance Imaging - methods</topic><topic>Magnetite Nanoparticles</topic><topic>Mice</topic><topic>Microscopy, Confocal</topic><topic>Rats</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Portnoy, Emma</creatorcontrib><creatorcontrib>Polyak, Boris</creatorcontrib><creatorcontrib>Inbar, Dorrit</creatorcontrib><creatorcontrib>Kenan, Gilad</creatorcontrib><creatorcontrib>Rai, Ahmad</creatorcontrib><creatorcontrib>Wehrli, Suzanne L</creatorcontrib><creatorcontrib>Roberts, Timothy P.L</creatorcontrib><creatorcontrib>Bishara, Ameer</creatorcontrib><creatorcontrib>Mann, Aniv</creatorcontrib><creatorcontrib>Shmuel, Miriam</creatorcontrib><creatorcontrib>Rozovsky, Katya</creatorcontrib><creatorcontrib>Itzhak, Gal</creatorcontrib><creatorcontrib>Hur, Tamir Ben</creatorcontrib><creatorcontrib>Magdassi, Shlomo</creatorcontrib><creatorcontrib>Ekstein, Dana</creatorcontrib><creatorcontrib>Eyal, Sara</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Nanomedicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Portnoy, Emma</au><au>Polyak, Boris</au><au>Inbar, Dorrit</au><au>Kenan, Gilad</au><au>Rai, Ahmad</au><au>Wehrli, Suzanne L</au><au>Roberts, Timothy P.L</au><au>Bishara, Ameer</au><au>Mann, Aniv</au><au>Shmuel, Miriam</au><au>Rozovsky, Katya</au><au>Itzhak, Gal</au><au>Hur, Tamir Ben</au><au>Magdassi, Shlomo</au><au>Ekstein, Dana</au><au>Eyal, Sara</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tracking inflammation in the epileptic rat brain by bi-functional fluorescent and magnetic nanoparticles</atitle><jtitle>Nanomedicine</jtitle><addtitle>Nanomedicine</addtitle><date>2016-07-01</date><risdate>2016</risdate><volume>12</volume><issue>5</issue><spage>1335</spage><epage>1345</epage><pages>1335-1345</pages><issn>1549-9634</issn><eissn>1549-9642</eissn><abstract>Abstract Correct localization of epileptic foci can improve surgical outcome in patients with drug-resistant seizures. 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| subjects | Animals Brain Epilepsy Epilepsy - surgery Hippocampus Humans Imaging Inflammation Internal Medicine Magnetic nanoparticles Magnetic Resonance Imaging Magnetic Resonance Imaging - methods Magnetite Nanoparticles Mice Microscopy, Confocal Rats |
| title | Tracking inflammation in the epileptic rat brain by bi-functional fluorescent and magnetic nanoparticles |
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