Fibrous polymer-grafted chitosan/clay composite beads as a carrier for immobilization of papain and its usability for mercury elimination
Papain, which is an industrially important enzyme, has been immobilized on fibrous polymer-modified composite beads, namely poly(methacrylic acid)-grafted chitosan/clay. Characterization studies have been done using FTIR and SEM analysis. Operating parameters such as pH and initial concentration of...
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| Veröffentlicht in: | Bioprocess and biosystems engineering 2016-07, Vol.39 (7), p.1137-1149 |
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| description | Papain, which is an industrially important enzyme, has been immobilized on fibrous polymer-modified composite beads, namely poly(methacrylic acid)-grafted chitosan/clay. Characterization studies have been done using FTIR and SEM analysis. Operating parameters such as pH and initial concentration of papain have been varied to obtain the finest papain immobilized polymer-modified composite beads. The immobilization capacity of composite beads has been determined as 34.47 ± 1.18 (
n
= 3) mg/g. The proteolytic activity of immobilized papain was operated using bovine serum albumin (BSA) and maximum velocity (
V
max
) and Michaelis–Menten constant (K
m
) values of the free and immobilized enzymes were determined using Lineweaver–Burk and Eadie–Hofstee equations. Usability of papain immobilized polymer-modified composite beads as adsorbents for the elimination of mercury was investigated. The maximum removal capacity of PIPMC beads has been found to be 4.88 ± 0.21 mg Hg/g when the initial metal concentration and weight of polymer-modified composite beads were 50 mg/L and 0.04 g at pH 7, respectively. Mercury removal performance of the papain immobilized polymer-modified composite beads was investigated in conjunction with Cu (II), Zn (II) and Cd (II) ions. The mercury adsorption capacity of papain immobilized polymer-modified composite beads was a slight reduction from 1.15 to 0.89 mg/g in presence of multiple metal salts. |
| doi_str_mv | 10.1007/s00449-016-1590-0 |
| format | Article |
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n
= 3) mg/g. The proteolytic activity of immobilized papain was operated using bovine serum albumin (BSA) and maximum velocity (
V
max
) and Michaelis–Menten constant (K
m
) values of the free and immobilized enzymes were determined using Lineweaver–Burk and Eadie–Hofstee equations. Usability of papain immobilized polymer-modified composite beads as adsorbents for the elimination of mercury was investigated. The maximum removal capacity of PIPMC beads has been found to be 4.88 ± 0.21 mg Hg/g when the initial metal concentration and weight of polymer-modified composite beads were 50 mg/L and 0.04 g at pH 7, respectively. Mercury removal performance of the papain immobilized polymer-modified composite beads was investigated in conjunction with Cu (II), Zn (II) and Cd (II) ions. The mercury adsorption capacity of papain immobilized polymer-modified composite beads was a slight reduction from 1.15 to 0.89 mg/g in presence of multiple metal salts.</description><identifier>ISSN: 1615-7591</identifier><identifier>EISSN: 1615-7605</identifier><identifier>DOI: 10.1007/s00449-016-1590-0</identifier><identifier>PMID: 27013506</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Adsorption ; Aluminum Silicates ; Bioengineering ; Biotechnology ; Chemistry ; Chemistry and Materials Science ; Chitosan - chemistry ; Clay ; Composite materials ; Environmental Engineering/Biotechnology ; Enzymes ; Enzymes, Immobilized - chemistry ; Food Science ; Hydrogen-Ion Concentration ; Industrial and Production Engineering ; Industrial Chemistry/Chemical Engineering ; Mercury ; Mercury - isolation & purification ; Metal concentrations ; Microscopy, Electron, Scanning ; Original Paper ; Papain - chemistry ; Polymers ; Polymers - chemistry ; Spectroscopy, Fourier Transform Infrared ; Temperature</subject><ispartof>Bioprocess and biosystems engineering, 2016-07, Vol.39 (7), p.1137-1149</ispartof><rights>Springer-Verlag Berlin Heidelberg 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c512t-582bd81e24ce214e5dc1e8fb544ab3fea763f825ab1a21892467d56dff51cc743</citedby><cites>FETCH-LOGICAL-c512t-582bd81e24ce214e5dc1e8fb544ab3fea763f825ab1a21892467d56dff51cc743</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00449-016-1590-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00449-016-1590-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51297</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27013506$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Metin, Ayşegül Ülkü</creatorcontrib><creatorcontrib>Alver, Erol</creatorcontrib><title>Fibrous polymer-grafted chitosan/clay composite beads as a carrier for immobilization of papain and its usability for mercury elimination</title><title>Bioprocess and biosystems engineering</title><addtitle>Bioprocess Biosyst Eng</addtitle><addtitle>Bioprocess Biosyst Eng</addtitle><description>Papain, which is an industrially important enzyme, has been immobilized on fibrous polymer-modified composite beads, namely poly(methacrylic acid)-grafted chitosan/clay. Characterization studies have been done using FTIR and SEM analysis. Operating parameters such as pH and initial concentration of papain have been varied to obtain the finest papain immobilized polymer-modified composite beads. The immobilization capacity of composite beads has been determined as 34.47 ± 1.18 (
n
= 3) mg/g. The proteolytic activity of immobilized papain was operated using bovine serum albumin (BSA) and maximum velocity (
V
max
) and Michaelis–Menten constant (K
m
) values of the free and immobilized enzymes were determined using Lineweaver–Burk and Eadie–Hofstee equations. Usability of papain immobilized polymer-modified composite beads as adsorbents for the elimination of mercury was investigated. The maximum removal capacity of PIPMC beads has been found to be 4.88 ± 0.21 mg Hg/g when the initial metal concentration and weight of polymer-modified composite beads were 50 mg/L and 0.04 g at pH 7, respectively. Mercury removal performance of the papain immobilized polymer-modified composite beads was investigated in conjunction with Cu (II), Zn (II) and Cd (II) ions. The mercury adsorption capacity of papain immobilized polymer-modified composite beads was a slight reduction from 1.15 to 0.89 mg/g in presence of multiple metal salts.</description><subject>Adsorption</subject><subject>Aluminum Silicates</subject><subject>Bioengineering</subject><subject>Biotechnology</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chitosan - chemistry</subject><subject>Clay</subject><subject>Composite materials</subject><subject>Environmental Engineering/Biotechnology</subject><subject>Enzymes</subject><subject>Enzymes, Immobilized - chemistry</subject><subject>Food Science</subject><subject>Hydrogen-Ion Concentration</subject><subject>Industrial and Production Engineering</subject><subject>Industrial Chemistry/Chemical Engineering</subject><subject>Mercury</subject><subject>Mercury - isolation & purification</subject><subject>Metal concentrations</subject><subject>Microscopy, Electron, Scanning</subject><subject>Original Paper</subject><subject>Papain - chemistry</subject><subject>Polymers</subject><subject>Polymers - chemistry</subject><subject>Spectroscopy, Fourier Transform Infrared</subject><subject>Temperature</subject><issn>1615-7591</issn><issn>1615-7605</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkVFr1zAUxYMobk4_gC8S8MWXutw0SdtHGU4HA1_0OdymNzOjbWrSPtRv4Lde_vtvIoIIgQTu75zDzWHsNYj3IERznoVQqqsEmAp0JyrxhJ2CAV01Ruinj2_dwQl7kfOtEKBbKZ6zE9kIqLUwp-zXZehT3DJf4rhPlKqbhH6lgbvvYY0Z53M34s5dnJaYw0q8Jxwyx3K4w5QCJe5j4mGaYh_G8BPXEGcePV9wwTBznAce1sy3jIf5ut_jJcltaec0hinM95qX7JnHMdOrh_uMfbv8-PXic3X95dPVxYfrymmQa1U26IcWSCpHEhTpwQG1vtdKYV97wsbUvpUae0AJbSeVaQZtBu81ONeo-oy9O_ouKf7YKK92CtnROOJM5SMstKI1Xa3q9v9o0zUlV0ld0Ld_obdxS3NZ5EAZVSsJXaHgSLkUc07k7ZLChGm3IOyhUnus1JZK7aFSK4rmzYPz1k80_FY8dlgAeQRyGc03lP6I_qfrHe7vrcE</recordid><startdate>20160701</startdate><enddate>20160701</enddate><creator>Metin, Ayşegül Ülkü</creator><creator>Alver, Erol</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>7QO</scope></search><sort><creationdate>20160701</creationdate><title>Fibrous polymer-grafted chitosan/clay composite beads as a carrier for immobilization of papain and its usability for mercury elimination</title><author>Metin, Ayşegül Ülkü ; Alver, Erol</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c512t-582bd81e24ce214e5dc1e8fb544ab3fea763f825ab1a21892467d56dff51cc743</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Adsorption</topic><topic>Aluminum Silicates</topic><topic>Bioengineering</topic><topic>Biotechnology</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chitosan - chemistry</topic><topic>Clay</topic><topic>Composite materials</topic><topic>Environmental Engineering/Biotechnology</topic><topic>Enzymes</topic><topic>Enzymes, Immobilized - chemistry</topic><topic>Food Science</topic><topic>Hydrogen-Ion Concentration</topic><topic>Industrial and Production Engineering</topic><topic>Industrial Chemistry/Chemical Engineering</topic><topic>Mercury</topic><topic>Mercury - isolation & purification</topic><topic>Metal concentrations</topic><topic>Microscopy, Electron, Scanning</topic><topic>Original Paper</topic><topic>Papain - chemistry</topic><topic>Polymers</topic><topic>Polymers - chemistry</topic><topic>Spectroscopy, Fourier Transform Infrared</topic><topic>Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Metin, Ayşegül Ülkü</creatorcontrib><creatorcontrib>Alver, Erol</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><jtitle>Bioprocess and biosystems engineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Metin, Ayşegül Ülkü</au><au>Alver, Erol</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fibrous polymer-grafted chitosan/clay composite beads as a carrier for immobilization of papain and its usability for mercury elimination</atitle><jtitle>Bioprocess and biosystems engineering</jtitle><stitle>Bioprocess Biosyst Eng</stitle><addtitle>Bioprocess Biosyst Eng</addtitle><date>2016-07-01</date><risdate>2016</risdate><volume>39</volume><issue>7</issue><spage>1137</spage><epage>1149</epage><pages>1137-1149</pages><issn>1615-7591</issn><eissn>1615-7605</eissn><abstract>Papain, which is an industrially important enzyme, has been immobilized on fibrous polymer-modified composite beads, namely poly(methacrylic acid)-grafted chitosan/clay. Characterization studies have been done using FTIR and SEM analysis. Operating parameters such as pH and initial concentration of papain have been varied to obtain the finest papain immobilized polymer-modified composite beads. The immobilization capacity of composite beads has been determined as 34.47 ± 1.18 (
n
= 3) mg/g. The proteolytic activity of immobilized papain was operated using bovine serum albumin (BSA) and maximum velocity (
V
max
) and Michaelis–Menten constant (K
m
) values of the free and immobilized enzymes were determined using Lineweaver–Burk and Eadie–Hofstee equations. Usability of papain immobilized polymer-modified composite beads as adsorbents for the elimination of mercury was investigated. The maximum removal capacity of PIPMC beads has been found to be 4.88 ± 0.21 mg Hg/g when the initial metal concentration and weight of polymer-modified composite beads were 50 mg/L and 0.04 g at pH 7, respectively. Mercury removal performance of the papain immobilized polymer-modified composite beads was investigated in conjunction with Cu (II), Zn (II) and Cd (II) ions. The mercury adsorption capacity of papain immobilized polymer-modified composite beads was a slight reduction from 1.15 to 0.89 mg/g in presence of multiple metal salts.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>27013506</pmid><doi>10.1007/s00449-016-1590-0</doi><tpages>13</tpages></addata></record> |
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| ispartof | Bioprocess and biosystems engineering, 2016-07, Vol.39 (7), p.1137-1149 |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Adsorption Aluminum Silicates Bioengineering Biotechnology Chemistry Chemistry and Materials Science Chitosan - chemistry Clay Composite materials Environmental Engineering/Biotechnology Enzymes Enzymes, Immobilized - chemistry Food Science Hydrogen-Ion Concentration Industrial and Production Engineering Industrial Chemistry/Chemical Engineering Mercury Mercury - isolation & purification Metal concentrations Microscopy, Electron, Scanning Original Paper Papain - chemistry Polymers Polymers - chemistry Spectroscopy, Fourier Transform Infrared Temperature |
| title | Fibrous polymer-grafted chitosan/clay composite beads as a carrier for immobilization of papain and its usability for mercury elimination |
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