A Rapid and Efficient Method for In Vitro Screening of Taro for Leaf Blight Disease Caused by Phytophthora colocasiae

Taro leaf blight caused by Phytophthora colocasiae presents the single biggest constraint for taro cultivation globally. To accelerate breeding and selection for disease resistance to leaf blight, it is important to develop bioassays which could differentiate resistant and susceptible cultivars effi...

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Veröffentlicht in:Journal of phytopathology 2016-08, Vol.164 (7-8), p.520-527
Hauptverfasser: Nath, Vishnu Sukumari, Basheer, Shyni, Jeeva, Muthulekshmi Lajapathy, Hegde, Vinayaka Mahabaleswar, Devi, Asha, Misra, Raj Shekar, Veena, Syamala Swayamvaran, Raj, Mithun
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container_end_page 527
container_issue 7-8
container_start_page 520
container_title Journal of phytopathology
container_volume 164
creator Nath, Vishnu Sukumari
Basheer, Shyni
Jeeva, Muthulekshmi Lajapathy
Hegde, Vinayaka Mahabaleswar
Devi, Asha
Misra, Raj Shekar
Veena, Syamala Swayamvaran
Raj, Mithun
description Taro leaf blight caused by Phytophthora colocasiae presents the single biggest constraint for taro cultivation globally. To accelerate breeding and selection for disease resistance to leaf blight, it is important to develop bioassays which could differentiate resistant and susceptible cultivars efficiently. In this study, thirty taro accessions and four released cultivars were evaluated for resistance to leaf blight using a modified floating leaf disc assay. A novel method for mass production of P. colocasiae zoospores was developed and used as inoculum for the assay. There were significant (P 
doi_str_mv 10.1111/jph.12477
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To accelerate breeding and selection for disease resistance to leaf blight, it is important to develop bioassays which could differentiate resistant and susceptible cultivars efficiently. In this study, thirty taro accessions and four released cultivars were evaluated for resistance to leaf blight using a modified floating leaf disc assay. A novel method for mass production of P. colocasiae zoospores was developed and used as inoculum for the assay. There were significant (P &lt; 0.05) differences among accessions in their response to P. colocasiae infection in the detached leaf assay. The accessions could be efficiently classified into various resistance groups based on a 0–4 score. Also, the assay results were consistent with the field evaluation scores of taro accessions. 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source Wiley Online Library Journals Frontfile Complete
subjects disease management
floating leaf disc assay
Phytophthora colocasiae
resistance screening
zoospore production
title A Rapid and Efficient Method for In Vitro Screening of Taro for Leaf Blight Disease Caused by Phytophthora colocasiae
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