A Rapid and Efficient Method for In Vitro Screening of Taro for Leaf Blight Disease Caused by Phytophthora colocasiae
Taro leaf blight caused by Phytophthora colocasiae presents the single biggest constraint for taro cultivation globally. To accelerate breeding and selection for disease resistance to leaf blight, it is important to develop bioassays which could differentiate resistant and susceptible cultivars effi...
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Veröffentlicht in: | Journal of phytopathology 2016-08, Vol.164 (7-8), p.520-527 |
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creator | Nath, Vishnu Sukumari Basheer, Shyni Jeeva, Muthulekshmi Lajapathy Hegde, Vinayaka Mahabaleswar Devi, Asha Misra, Raj Shekar Veena, Syamala Swayamvaran Raj, Mithun |
description | Taro leaf blight caused by Phytophthora colocasiae presents the single biggest constraint for taro cultivation globally. To accelerate breeding and selection for disease resistance to leaf blight, it is important to develop bioassays which could differentiate resistant and susceptible cultivars efficiently. In this study, thirty taro accessions and four released cultivars were evaluated for resistance to leaf blight using a modified floating leaf disc assay. A novel method for mass production of P. colocasiae zoospores was developed and used as inoculum for the assay. There were significant (P |
doi_str_mv | 10.1111/jph.12477 |
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To accelerate breeding and selection for disease resistance to leaf blight, it is important to develop bioassays which could differentiate resistant and susceptible cultivars efficiently. In this study, thirty taro accessions and four released cultivars were evaluated for resistance to leaf blight using a modified floating leaf disc assay. A novel method for mass production of P. colocasiae zoospores was developed and used as inoculum for the assay. There were significant (P < 0.05) differences among accessions in their response to P. colocasiae infection in the detached leaf assay. The accessions could be efficiently classified into various resistance groups based on a 0–4 score. Also, the assay results were consistent with the field evaluation scores of taro accessions. Thus, this study reports the development of a rapid, simple and repeatable assay that can be used to screen large numbers of taro cultivars for resistance to P. colocasiae.</description><identifier>ISSN: 0931-1785</identifier><identifier>EISSN: 1439-0434</identifier><identifier>DOI: 10.1111/jph.12477</identifier><language>eng</language><publisher>Berlin: Blackwell Publishing Ltd</publisher><subject>disease management ; floating leaf disc assay ; Phytophthora colocasiae ; resistance screening ; zoospore production</subject><ispartof>Journal of phytopathology, 2016-08, Vol.164 (7-8), p.520-527</ispartof><rights>2016 Blackwell Verlag GmbH</rights><rights>Copyright © 2016 Blackwell Verlag GmbH</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3687-450cf40c0db377e9019d620972454f7bde6e6bb688419568d67b7392e488156a3</citedby><cites>FETCH-LOGICAL-c3687-450cf40c0db377e9019d620972454f7bde6e6bb688419568d67b7392e488156a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjph.12477$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjph.12477$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids></links><search><creatorcontrib>Nath, Vishnu Sukumari</creatorcontrib><creatorcontrib>Basheer, Shyni</creatorcontrib><creatorcontrib>Jeeva, Muthulekshmi Lajapathy</creatorcontrib><creatorcontrib>Hegde, Vinayaka Mahabaleswar</creatorcontrib><creatorcontrib>Devi, Asha</creatorcontrib><creatorcontrib>Misra, Raj Shekar</creatorcontrib><creatorcontrib>Veena, Syamala Swayamvaran</creatorcontrib><creatorcontrib>Raj, Mithun</creatorcontrib><title>A Rapid and Efficient Method for In Vitro Screening of Taro for Leaf Blight Disease Caused by Phytophthora colocasiae</title><title>Journal of phytopathology</title><addtitle>J Phytopathol</addtitle><description>Taro leaf blight caused by Phytophthora colocasiae presents the single biggest constraint for taro cultivation globally. To accelerate breeding and selection for disease resistance to leaf blight, it is important to develop bioassays which could differentiate resistant and susceptible cultivars efficiently. In this study, thirty taro accessions and four released cultivars were evaluated for resistance to leaf blight using a modified floating leaf disc assay. A novel method for mass production of P. colocasiae zoospores was developed and used as inoculum for the assay. There were significant (P < 0.05) differences among accessions in their response to P. colocasiae infection in the detached leaf assay. The accessions could be efficiently classified into various resistance groups based on a 0–4 score. Also, the assay results were consistent with the field evaluation scores of taro accessions. Thus, this study reports the development of a rapid, simple and repeatable assay that can be used to screen large numbers of taro cultivars for resistance to P. colocasiae.</description><subject>disease management</subject><subject>floating leaf disc assay</subject><subject>Phytophthora colocasiae</subject><subject>resistance screening</subject><subject>zoospore production</subject><issn>0931-1785</issn><issn>1439-0434</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNp1kU9v1DAQxS0EEkvpgW9giQsc0tqxYzvHsv2LlrItBY6W44wbL2kc7ESw375eFjggMZeRZn7vzUgPoVeUHNFcx5uxO6Ill_IJWlDO6oJwxp-iBakZLahU1XP0IqUNISVhhCzQfIJvzehbbIYWnznnrYdhwh9g6kKLXYj4asBf_BQD_mQjwOCHexwcvjN5sluvwDj8rvf33YRPfQKTAC_NnKDFzRavu-0Uxi6bRYNt6IM1yRt4iZ450yc4_N0P0Ofzs7vlZbH6eHG1PFkVlgklC14R6zixpG2YlFATWreiJLUsecWdbFoQIJpGKMVpXQnVCtlIVpfAlaKVMOwAvdn7jjF8nyFN-sEnC31vBghz0lQRtVMTltHX_6CbMMchf7ejSiaFEjJTb_eUjSGlCE6P0T-YuNWU6F0AOgegfwWQ2eM9-8P3sP0_qN-vL_8oir3Cpwl-_lWY-E3n27LSX68v9M2SXYvzU67X7BH1rpQZ</recordid><startdate>201608</startdate><enddate>201608</enddate><creator>Nath, Vishnu Sukumari</creator><creator>Basheer, Shyni</creator><creator>Jeeva, Muthulekshmi Lajapathy</creator><creator>Hegde, Vinayaka Mahabaleswar</creator><creator>Devi, Asha</creator><creator>Misra, Raj Shekar</creator><creator>Veena, Syamala Swayamvaran</creator><creator>Raj, Mithun</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>201608</creationdate><title>A Rapid and Efficient Method for In Vitro Screening of Taro for Leaf Blight Disease Caused by Phytophthora colocasiae</title><author>Nath, Vishnu Sukumari ; Basheer, Shyni ; Jeeva, Muthulekshmi Lajapathy ; Hegde, Vinayaka Mahabaleswar ; Devi, Asha ; Misra, Raj Shekar ; Veena, Syamala Swayamvaran ; Raj, Mithun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3687-450cf40c0db377e9019d620972454f7bde6e6bb688419568d67b7392e488156a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>disease management</topic><topic>floating leaf disc assay</topic><topic>Phytophthora colocasiae</topic><topic>resistance screening</topic><topic>zoospore production</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nath, Vishnu Sukumari</creatorcontrib><creatorcontrib>Basheer, Shyni</creatorcontrib><creatorcontrib>Jeeva, Muthulekshmi Lajapathy</creatorcontrib><creatorcontrib>Hegde, Vinayaka Mahabaleswar</creatorcontrib><creatorcontrib>Devi, Asha</creatorcontrib><creatorcontrib>Misra, Raj Shekar</creatorcontrib><creatorcontrib>Veena, Syamala Swayamvaran</creatorcontrib><creatorcontrib>Raj, Mithun</creatorcontrib><collection>Istex</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of phytopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nath, Vishnu Sukumari</au><au>Basheer, Shyni</au><au>Jeeva, Muthulekshmi Lajapathy</au><au>Hegde, Vinayaka Mahabaleswar</au><au>Devi, Asha</au><au>Misra, Raj Shekar</au><au>Veena, Syamala Swayamvaran</au><au>Raj, Mithun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Rapid and Efficient Method for In Vitro Screening of Taro for Leaf Blight Disease Caused by Phytophthora colocasiae</atitle><jtitle>Journal of phytopathology</jtitle><addtitle>J Phytopathol</addtitle><date>2016-08</date><risdate>2016</risdate><volume>164</volume><issue>7-8</issue><spage>520</spage><epage>527</epage><pages>520-527</pages><issn>0931-1785</issn><eissn>1439-0434</eissn><abstract>Taro leaf blight caused by Phytophthora colocasiae presents the single biggest constraint for taro cultivation globally. To accelerate breeding and selection for disease resistance to leaf blight, it is important to develop bioassays which could differentiate resistant and susceptible cultivars efficiently. In this study, thirty taro accessions and four released cultivars were evaluated for resistance to leaf blight using a modified floating leaf disc assay. A novel method for mass production of P. colocasiae zoospores was developed and used as inoculum for the assay. There were significant (P < 0.05) differences among accessions in their response to P. colocasiae infection in the detached leaf assay. The accessions could be efficiently classified into various resistance groups based on a 0–4 score. Also, the assay results were consistent with the field evaluation scores of taro accessions. Thus, this study reports the development of a rapid, simple and repeatable assay that can be used to screen large numbers of taro cultivars for resistance to P. colocasiae.</abstract><cop>Berlin</cop><pub>Blackwell Publishing Ltd</pub><doi>10.1111/jph.12477</doi><tpages>8</tpages></addata></record> |
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subjects | disease management floating leaf disc assay Phytophthora colocasiae resistance screening zoospore production |
title | A Rapid and Efficient Method for In Vitro Screening of Taro for Leaf Blight Disease Caused by Phytophthora colocasiae |
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