MHC class IIα polymorphisms and their association with resistance/susceptibility to Singapore grouper iridovirus (SGIV) in orange-spotted grouper, Epinephelus coioides
The major histocompatibility complex (MHC) is a crucial component of the immune systems of vertebrate animals and is associated with their resistance/susceptibility to pathogenic diseases. In this study, the association between MHC IIα gene polymorphisms and disease resistance/susceptibility was ana...
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| Veröffentlicht in: | Aquaculture 2016-09, Vol.462, p.10-16 |
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| creator | Yang, Min Wei, Jingguang Li, Pengfei Wei, Shina Huang, Youhua Qin, Qiwei |
| description | The major histocompatibility complex (MHC) is a crucial component of the immune systems of vertebrate animals and is associated with their resistance/susceptibility to pathogenic diseases. In this study, the association between MHC IIα gene polymorphisms and disease resistance/susceptibility was analyzed in the orange-spotted grouper (Epinephelus coioides), by challenging it with Singapore grouper iridovirus (SGIV). Thirty-seven alleles showing high levels of polymorphism (24.7% mutation sites) were isolated from 80 individuals. In the peptide-binding region, the ratio of nonsynonymous (dN) substitutions to synonymous (dS) substitutions was 1.427 (>1), suggesting that the loci are evolving under positive balancing selection. Eight alleles were selected to estimate their distributions in high-resistance (HR) and high-susceptibility (HS) groups of fish. The EPCO-DAA*0101 allele was significantly associated with susceptibility to SGIV infection (P |
| doi_str_mv | 10.1016/j.aquaculture.2016.04.026 |
| format | Article |
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Molecular marker-assisted selective breeding program.
•We detected the MHC class IIα polymorphisms and their association with resistance/susceptibility to iridovirus in grouper•MHC allele EPCO-DAA*1101 was associated with resistance to SGIV, allele EPCO-DAA*0101 was associated with susceptibility•These markers could be useful for the MAS selective breeding of the orange-spotted grouper.</description><identifier>ISSN: 0044-8486</identifier><identifier>EISSN: 1873-5622</identifier><identifier>DOI: 10.1016/j.aquaculture.2016.04.026</identifier><language>eng</language><publisher>Elsevier B.V</publisher><subject>Disease resistance ; Epinephelus coioides ; Major histocompatibility complex IIα ; Marine ; Orange-spotted grouper ; Selective breeding program ; Singapore Grouper Iridovirus</subject><ispartof>Aquaculture, 2016-09, Vol.462, p.10-16</ispartof><rights>2016 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c354t-f7d35aab1697d59c160176ee08e949562d06b8a658aded410ed4c3109c47800c3</citedby><cites>FETCH-LOGICAL-c354t-f7d35aab1697d59c160176ee08e949562d06b8a658aded410ed4c3109c47800c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0044848616302113$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids></links><search><creatorcontrib>Yang, Min</creatorcontrib><creatorcontrib>Wei, Jingguang</creatorcontrib><creatorcontrib>Li, Pengfei</creatorcontrib><creatorcontrib>Wei, Shina</creatorcontrib><creatorcontrib>Huang, Youhua</creatorcontrib><creatorcontrib>Qin, Qiwei</creatorcontrib><title>MHC class IIα polymorphisms and their association with resistance/susceptibility to Singapore grouper iridovirus (SGIV) in orange-spotted grouper, Epinephelus coioides</title><title>Aquaculture</title><description>The major histocompatibility complex (MHC) is a crucial component of the immune systems of vertebrate animals and is associated with their resistance/susceptibility to pathogenic diseases. In this study, the association between MHC IIα gene polymorphisms and disease resistance/susceptibility was analyzed in the orange-spotted grouper (Epinephelus coioides), by challenging it with Singapore grouper iridovirus (SGIV). Thirty-seven alleles showing high levels of polymorphism (24.7% mutation sites) were isolated from 80 individuals. In the peptide-binding region, the ratio of nonsynonymous (dN) substitutions to synonymous (dS) substitutions was 1.427 (>1), suggesting that the loci are evolving under positive balancing selection. Eight alleles were selected to estimate their distributions in high-resistance (HR) and high-susceptibility (HS) groups of fish. The EPCO-DAA*0101 allele was significantly associated with susceptibility to SGIV infection (P<0.05) and the EPCO-DAA*0104, EPCO-DAA*0601, EPCO-DAA*1101, and EPCO-DAA*1201 alleles were significantly associated with resistance to SGIV (P<0.05 for each). To confirm these correlations, an additional independent challenge experiment was performed in the Wenchang population of the orange-spotted grouper. The frequency distribution showed that the EPCO-DAA*0101 allele was significantly more frequent in the Wenchang HS (WC-HS) group than in the WC-HR group (P<0.05), whereas the EPCO-DAA*1101 allele was significantly more frequent in the WC-HR group than in the WC-HS group (P<0.05), consistent with the first challenge. However, the frequency distributions of the EPCO-DAA*0104, EPCO-DAA*0601, and EPCO-DAA*1201 alleles did not differ significantly between the WC-HR and WC-HS groups (P>0.05). These results indicate that the EPCO-DAA*0101 allele confers susceptibility to SGIV infection, whereas the EPCO-DAA*1101 allele confers resistance to it. These disease-resistance-related MHC markers could be useful for the molecular-marker-assisted selective breeding of the orange-spotted grouper.
Molecular marker-assisted selective breeding program.
•We detected the MHC class IIα polymorphisms and their association with resistance/susceptibility to iridovirus in grouper•MHC allele EPCO-DAA*1101 was associated with resistance to SGIV, allele EPCO-DAA*0101 was associated with susceptibility•These markers could be useful for the MAS selective breeding of the orange-spotted grouper.</description><subject>Disease resistance</subject><subject>Epinephelus coioides</subject><subject>Major histocompatibility complex IIα</subject><subject>Marine</subject><subject>Orange-spotted grouper</subject><subject>Selective breeding program</subject><subject>Singapore Grouper Iridovirus</subject><issn>0044-8486</issn><issn>1873-5622</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNqNkcFuEzEQhlcIJELhHcytSOzWznq93iOKShupiEOBq-XYk2Sizdr1eIvyRlx5EZ4JVwGJI5cZafT9vzT_X1VvBW8EF-rq0NiH2bp5zHOCZllODZcNX6pn1ULovq07tVw-rxacS1lrqdXL6hXRgXOuVCcW1Y9PtyvmRkvE1utfP1kM4-kYUtwjHYnZybO8B0ysAMGhzRgm9h3zniUgpGwnB1c0k4OYcYMj5hPLgd3jtLMxJGC7FOYIiWFCHx4xzcQu72_W394xnFhIdtpBTTHkDP4v-55dR5wg7mEstAsY0AO9rl5s7Ujw5s--qL5-vP6yuq3vPt-sVx_uatd2Mtfb3redtRuhht53gxOKi14BcA2DHEoYnquNtqrT1oOXgpfhWsEHJ3vNuWsvqsuzb0zhYQbK5ojlvXG0E4SZjNBcK636vi_ocEZdCkQJtiYmPNp0MoKbp3bMwfzTjnlqx3BpSjtFuzprofzyiJAMOYSSpscELhsf8D9cfgOMEaQ1</recordid><startdate>20160901</startdate><enddate>20160901</enddate><creator>Yang, Min</creator><creator>Wei, Jingguang</creator><creator>Li, Pengfei</creator><creator>Wei, Shina</creator><creator>Huang, Youhua</creator><creator>Qin, Qiwei</creator><general>Elsevier B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7ST</scope><scope>7TN</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H94</scope><scope>H95</scope><scope>H98</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>P64</scope><scope>SOI</scope></search><sort><creationdate>20160901</creationdate><title>MHC class IIα polymorphisms and their association with resistance/susceptibility to Singapore grouper iridovirus (SGIV) in orange-spotted grouper, Epinephelus coioides</title><author>Yang, Min ; Wei, Jingguang ; Li, Pengfei ; Wei, Shina ; Huang, Youhua ; Qin, Qiwei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c354t-f7d35aab1697d59c160176ee08e949562d06b8a658aded410ed4c3109c47800c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Disease resistance</topic><topic>Epinephelus coioides</topic><topic>Major histocompatibility complex IIα</topic><topic>Marine</topic><topic>Orange-spotted grouper</topic><topic>Selective breeding program</topic><topic>Singapore Grouper Iridovirus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, Min</creatorcontrib><creatorcontrib>Wei, Jingguang</creatorcontrib><creatorcontrib>Li, Pengfei</creatorcontrib><creatorcontrib>Wei, Shina</creatorcontrib><creatorcontrib>Huang, Youhua</creatorcontrib><creatorcontrib>Qin, Qiwei</creatorcontrib><collection>CrossRef</collection><collection>Environment Abstracts</collection><collection>Oceanic Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><jtitle>Aquaculture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Min</au><au>Wei, Jingguang</au><au>Li, Pengfei</au><au>Wei, Shina</au><au>Huang, Youhua</au><au>Qin, Qiwei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>MHC class IIα polymorphisms and their association with resistance/susceptibility to Singapore grouper iridovirus (SGIV) in orange-spotted grouper, Epinephelus coioides</atitle><jtitle>Aquaculture</jtitle><date>2016-09-01</date><risdate>2016</risdate><volume>462</volume><spage>10</spage><epage>16</epage><pages>10-16</pages><issn>0044-8486</issn><eissn>1873-5622</eissn><abstract>The major histocompatibility complex (MHC) is a crucial component of the immune systems of vertebrate animals and is associated with their resistance/susceptibility to pathogenic diseases. In this study, the association between MHC IIα gene polymorphisms and disease resistance/susceptibility was analyzed in the orange-spotted grouper (Epinephelus coioides), by challenging it with Singapore grouper iridovirus (SGIV). Thirty-seven alleles showing high levels of polymorphism (24.7% mutation sites) were isolated from 80 individuals. In the peptide-binding region, the ratio of nonsynonymous (dN) substitutions to synonymous (dS) substitutions was 1.427 (>1), suggesting that the loci are evolving under positive balancing selection. Eight alleles were selected to estimate their distributions in high-resistance (HR) and high-susceptibility (HS) groups of fish. The EPCO-DAA*0101 allele was significantly associated with susceptibility to SGIV infection (P<0.05) and the EPCO-DAA*0104, EPCO-DAA*0601, EPCO-DAA*1101, and EPCO-DAA*1201 alleles were significantly associated with resistance to SGIV (P<0.05 for each). To confirm these correlations, an additional independent challenge experiment was performed in the Wenchang population of the orange-spotted grouper. The frequency distribution showed that the EPCO-DAA*0101 allele was significantly more frequent in the Wenchang HS (WC-HS) group than in the WC-HR group (P<0.05), whereas the EPCO-DAA*1101 allele was significantly more frequent in the WC-HR group than in the WC-HS group (P<0.05), consistent with the first challenge. However, the frequency distributions of the EPCO-DAA*0104, EPCO-DAA*0601, and EPCO-DAA*1201 alleles did not differ significantly between the WC-HR and WC-HS groups (P>0.05). These results indicate that the EPCO-DAA*0101 allele confers susceptibility to SGIV infection, whereas the EPCO-DAA*1101 allele confers resistance to it. These disease-resistance-related MHC markers could be useful for the molecular-marker-assisted selective breeding of the orange-spotted grouper.
Molecular marker-assisted selective breeding program.
•We detected the MHC class IIα polymorphisms and their association with resistance/susceptibility to iridovirus in grouper•MHC allele EPCO-DAA*1101 was associated with resistance to SGIV, allele EPCO-DAA*0101 was associated with susceptibility•These markers could be useful for the MAS selective breeding of the orange-spotted grouper.</abstract><pub>Elsevier B.V</pub><doi>10.1016/j.aquaculture.2016.04.026</doi><tpages>7</tpages></addata></record> |
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| subjects | Disease resistance Epinephelus coioides Major histocompatibility complex IIα Marine Orange-spotted grouper Selective breeding program Singapore Grouper Iridovirus |
| title | MHC class IIα polymorphisms and their association with resistance/susceptibility to Singapore grouper iridovirus (SGIV) in orange-spotted grouper, Epinephelus coioides |
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