Embryotoxicity Test of Restoration Materials using ES Cells Enclosed with Collagen Gel

We examined the embryotoxicity of dental materials using a three-dimension protocol using porcine collagen gel. Although cell differentiation was hardly observed in dental amalgam, the cell survival ratio of 3T3 cells was the highest level. Glass ionomer cement showed the highest differentiation ratio and there was a tendency that the cell survival ratio of 3T3 cells was the lowest. In the two types of light-cured composite resin showed a moderate embryotoxicity. In this test protocol, it became possible to expose dental materials after curing to the ES cells without using solvents thanks to the application of a three-dimensional culture method using collagen gel. Although this modified EST method has not been subjected to validation test, it can be positively assessed to some extent for in vitro examination of the embryotoxicity level of dental materials.