Influence on the Long-term Differentiation Culture of ES-D3 Cells with String-like Collagen Scaffolds Derived from Tilapia Scales

In the field of regenerative medicine, tissues and organs differentiated from iPS cells are implanted into the body. For this purpose, scaffolds using collagen derived from fish, evolutionarily distant from humans, are safer than those using mammalian (porcine) collagen. In the present study, the di...

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Veröffentlicht in:Journal of Oral Tissue Engineering 2015, Vol.13(3), pp.125-130
Hauptverfasser: IMAI, Koichi, NISHIKAWA, Tetsunari, MORITA, Shosuke, ISEKI, Tomio, YOSHIDA, Hiroaki, MATSUMOTO, Kazuhiro, SHIDA, Muneyasu, OGAWA, Fumiya, SUESE, Kazuhiko
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container_end_page 130
container_issue 3
container_start_page 125
container_title Journal of Oral Tissue Engineering
container_volume 13
creator IMAI, Koichi
NISHIKAWA, Tetsunari
MORITA, Shosuke
ISEKI, Tomio
YOSHIDA, Hiroaki
MATSUMOTO, Kazuhiro
SHIDA, Muneyasu
OGAWA, Fumiya
SUESE, Kazuhiko
description In the field of regenerative medicine, tissues and organs differentiated from iPS cells are implanted into the body. For this purpose, scaffolds using collagen derived from fish, evolutionarily distant from humans, are safer than those using mammalian (porcine) collagen. In the present study, the differentiation of mouse-derived ES cells was examined using string-like porous collagen derived from tilapia that inhabits tropical and subtropical regions. Cell differentiation into bone or cartilage requires 30-day culture. Whether or not the string-like collagen could withstand the long-term culture was examined, demonstrating that 86% of the collagen retained a gel form during the culture without any effect on ES cell differentiation.
doi_str_mv 10.11223/jarde.13.125
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source Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese
subjects caffold
ES cells
marine collagen
porous collagen
Tilapia
title Influence on the Long-term Differentiation Culture of ES-D3 Cells with String-like Collagen Scaffolds Derived from Tilapia Scales
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