Influence on the Long-term Differentiation Culture of ES-D3 Cells with String-like Collagen Scaffolds Derived from Tilapia Scales
In the field of regenerative medicine, tissues and organs differentiated from iPS cells are implanted into the body. For this purpose, scaffolds using collagen derived from fish, evolutionarily distant from humans, are safer than those using mammalian (porcine) collagen. In the present study, the di...
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Veröffentlicht in: | Journal of Oral Tissue Engineering 2015, Vol.13(3), pp.125-130 |
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container_title | Journal of Oral Tissue Engineering |
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creator | IMAI, Koichi NISHIKAWA, Tetsunari MORITA, Shosuke ISEKI, Tomio YOSHIDA, Hiroaki MATSUMOTO, Kazuhiro SHIDA, Muneyasu OGAWA, Fumiya SUESE, Kazuhiko |
description | In the field of regenerative medicine, tissues and organs differentiated from iPS cells are implanted into the body. For this purpose, scaffolds using collagen derived from fish, evolutionarily distant from humans, are safer than those using mammalian (porcine) collagen. In the present study, the differentiation of mouse-derived ES cells was examined using string-like porous collagen derived from tilapia that inhabits tropical and subtropical regions. Cell differentiation into bone or cartilage requires 30-day culture. Whether or not the string-like collagen could withstand the long-term culture was examined, demonstrating that 86% of the collagen retained a gel form during the culture without any effect on ES cell differentiation. |
doi_str_mv | 10.11223/jarde.13.125 |
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Oral Tissue Engin.</addtitle><description>In the field of regenerative medicine, tissues and organs differentiated from iPS cells are implanted into the body. For this purpose, scaffolds using collagen derived from fish, evolutionarily distant from humans, are safer than those using mammalian (porcine) collagen. In the present study, the differentiation of mouse-derived ES cells was examined using string-like porous collagen derived from tilapia that inhabits tropical and subtropical regions. Cell differentiation into bone or cartilage requires 30-day culture. 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Oral Tissue Engin.</addtitle><date>2015-01-01</date><risdate>2015</risdate><volume>13</volume><issue>3</issue><spage>125</spage><epage>130</epage><pages>125-130</pages><issn>1348-9623</issn><eissn>1880-0823</eissn><abstract>In the field of regenerative medicine, tissues and organs differentiated from iPS cells are implanted into the body. For this purpose, scaffolds using collagen derived from fish, evolutionarily distant from humans, are safer than those using mammalian (porcine) collagen. In the present study, the differentiation of mouse-derived ES cells was examined using string-like porous collagen derived from tilapia that inhabits tropical and subtropical regions. Cell differentiation into bone or cartilage requires 30-day culture. Whether or not the string-like collagen could withstand the long-term culture was examined, demonstrating that 86% of the collagen retained a gel form during the culture without any effect on ES cell differentiation.</abstract><cop>Okayama</cop><pub>Japanese Association of Regenerative Dentistry</pub><doi>10.11223/jarde.13.125</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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subjects | caffold ES cells marine collagen porous collagen Tilapia |
title | Influence on the Long-term Differentiation Culture of ES-D3 Cells with String-like Collagen Scaffolds Derived from Tilapia Scales |
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