Regulatory function of Arabidopsis lipid transfer protein 1 (LTP1) in ethylene response and signaling
Ethylene as a gaseous plant hormone is directly involved in various processes during plant growth and development. Much is known regarding the ethylene receptors and regulatory factors in the ethylene signal transduction pathway. In Arabidopsis thaliana , REVERSION-TO-ETHYLENE SENSITIVITY1 (RTE1) ca...
Gespeichert in:
| Veröffentlicht in: | Plant molecular biology 2016-07, Vol.91 (4-5), p.471-484 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 484 |
|---|---|
| container_issue | 4-5 |
| container_start_page | 471 |
| container_title | Plant molecular biology |
| container_volume | 91 |
| creator | Wang, Honglin Sun, Yue Chang, Jianhong Zheng, Fangfang Pei, Haixia Yi, Yanjun Chang, Caren Dong, Chun-Hai |
| description | Ethylene as a gaseous plant hormone is directly involved in various processes during plant growth and development. Much is known regarding the ethylene receptors and regulatory factors in the ethylene signal transduction pathway. In
Arabidopsis thaliana
, REVERSION-TO-ETHYLENE SENSITIVITY1 (RTE1) can interact with and positively regulates the ethylene receptor ETHYLENE RESPONSE1 (ETR1). In this study we report the identification and characterization of an RTE1-interacting protein, a putative Arabidopsis lipid transfer protein 1 (LTP1) of unknown function. Through bimolecular fluorescence complementation, a direct molecular interaction between LTP1 and RTE1 was verified in planta. Analysis of an LTP1-GFP fusion in transgenic plants and plasmolysis experiments revealed that LTP1 is localized to the cytoplasm. Analysis of ethylene responses showed that the
ltp1
knockout is hypersensitive to 1-aminocyclopropanecarboxylic acid (ACC), while
LTP1
overexpression confers insensitivity. Analysis of double mutants
etr1
-
2 ltp1
and
rte1
-
3 ltp1
demonstrates a regulatory function of LTP1 in ethylene receptor signaling through the molecular association with RTE1. This study uncovers a novel function of Arabidopsis LTP1 in the regulation of ethylene response and signaling. |
| doi_str_mv | 10.1007/s11103-016-0482-7 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1808670803</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>4095046551</sourcerecordid><originalsourceid>FETCH-LOGICAL-c405t-602df772496ed408d6ca563b03240d10a0d69c1b266f9f45e3400a9802a0a99e3</originalsourceid><addsrcrecordid>eNqFkU1r3DAQhkVpabbb_oBeiqCX9OB29GHJOobQL1hICOlZaK3xVsEruZJ92H8fBaelFEqYwwzM874j8RLylsFHBqA_FcYYiAaYakB2vNHPyIa1WjQt8O452dSFbqRk_Iy8KuUOoKqEeknOuAajDYgNwRs8LKObUz7RYYn9HFKkaaAX2e2DT1MJhY5hCp7O2cUyYKZTTjOGSBk9391esw-0zjj_PI0YkWYsU4oFqYuelnCIbgzx8Jq8GNxY8M1j35IfXz7fXn5rdldfv19e7JpeQjs3CrgftObSKPQSOq961yqxB8EleAYOvDI923OlBjPIFoUEcKYD7mozKLbkfPWtb_y1YJntMZQex9FFTEuxrINOaehAPI1q0xkjWK0tef8PepeWXH-2Uhw0B14ptlJ9TqVkHOyUw9Hlk2VgH-Kya1y2pmIf4rK6at49Oi_7I_o_it_5VICvQKmreMD81-n_ut4Dq7mdmw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1798207202</pqid></control><display><type>article</type><title>Regulatory function of Arabidopsis lipid transfer protein 1 (LTP1) in ethylene response and signaling</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Wang, Honglin ; Sun, Yue ; Chang, Jianhong ; Zheng, Fangfang ; Pei, Haixia ; Yi, Yanjun ; Chang, Caren ; Dong, Chun-Hai</creator><creatorcontrib>Wang, Honglin ; Sun, Yue ; Chang, Jianhong ; Zheng, Fangfang ; Pei, Haixia ; Yi, Yanjun ; Chang, Caren ; Dong, Chun-Hai</creatorcontrib><description>Ethylene as a gaseous plant hormone is directly involved in various processes during plant growth and development. Much is known regarding the ethylene receptors and regulatory factors in the ethylene signal transduction pathway. In
Arabidopsis thaliana
, REVERSION-TO-ETHYLENE SENSITIVITY1 (RTE1) can interact with and positively regulates the ethylene receptor ETHYLENE RESPONSE1 (ETR1). In this study we report the identification and characterization of an RTE1-interacting protein, a putative Arabidopsis lipid transfer protein 1 (LTP1) of unknown function. Through bimolecular fluorescence complementation, a direct molecular interaction between LTP1 and RTE1 was verified in planta. Analysis of an LTP1-GFP fusion in transgenic plants and plasmolysis experiments revealed that LTP1 is localized to the cytoplasm. Analysis of ethylene responses showed that the
ltp1
knockout is hypersensitive to 1-aminocyclopropanecarboxylic acid (ACC), while
LTP1
overexpression confers insensitivity. Analysis of double mutants
etr1
-
2 ltp1
and
rte1
-
3 ltp1
demonstrates a regulatory function of LTP1 in ethylene receptor signaling through the molecular association with RTE1. This study uncovers a novel function of Arabidopsis LTP1 in the regulation of ethylene response and signaling.</description><identifier>ISSN: 0167-4412</identifier><identifier>EISSN: 1573-5028</identifier><identifier>DOI: 10.1007/s11103-016-0482-7</identifier><identifier>PMID: 27097903</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Arabidopsis ; Arabidopsis - genetics ; Arabidopsis - metabolism ; Arabidopsis Proteins - metabolism ; Arabidopsis thaliana ; Biochemistry ; Biomedical and Life Sciences ; Ethylenes - metabolism ; Flowers & plants ; Gene Expression Regulation, Plant ; Life Sciences ; Mutation - genetics ; Plant biology ; Plant growth ; Plant Pathology ; Plant Sciences ; Plants, Genetically Modified ; Protein Binding ; Protein expression ; Protein Transport ; Signal Transduction ; Subcellular Fractions - metabolism ; Transgenic plants</subject><ispartof>Plant molecular biology, 2016-07, Vol.91 (4-5), p.471-484</ispartof><rights>Springer Science+Business Media Dordrecht 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-602df772496ed408d6ca563b03240d10a0d69c1b266f9f45e3400a9802a0a99e3</citedby><cites>FETCH-LOGICAL-c405t-602df772496ed408d6ca563b03240d10a0d69c1b266f9f45e3400a9802a0a99e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11103-016-0482-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11103-016-0482-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27923,27924,41487,42556,51318</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27097903$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Honglin</creatorcontrib><creatorcontrib>Sun, Yue</creatorcontrib><creatorcontrib>Chang, Jianhong</creatorcontrib><creatorcontrib>Zheng, Fangfang</creatorcontrib><creatorcontrib>Pei, Haixia</creatorcontrib><creatorcontrib>Yi, Yanjun</creatorcontrib><creatorcontrib>Chang, Caren</creatorcontrib><creatorcontrib>Dong, Chun-Hai</creatorcontrib><title>Regulatory function of Arabidopsis lipid transfer protein 1 (LTP1) in ethylene response and signaling</title><title>Plant molecular biology</title><addtitle>Plant Mol Biol</addtitle><addtitle>Plant Mol Biol</addtitle><description>Ethylene as a gaseous plant hormone is directly involved in various processes during plant growth and development. Much is known regarding the ethylene receptors and regulatory factors in the ethylene signal transduction pathway. In
Arabidopsis thaliana
, REVERSION-TO-ETHYLENE SENSITIVITY1 (RTE1) can interact with and positively regulates the ethylene receptor ETHYLENE RESPONSE1 (ETR1). In this study we report the identification and characterization of an RTE1-interacting protein, a putative Arabidopsis lipid transfer protein 1 (LTP1) of unknown function. Through bimolecular fluorescence complementation, a direct molecular interaction between LTP1 and RTE1 was verified in planta. Analysis of an LTP1-GFP fusion in transgenic plants and plasmolysis experiments revealed that LTP1 is localized to the cytoplasm. Analysis of ethylene responses showed that the
ltp1
knockout is hypersensitive to 1-aminocyclopropanecarboxylic acid (ACC), while
LTP1
overexpression confers insensitivity. Analysis of double mutants
etr1
-
2 ltp1
and
rte1
-
3 ltp1
demonstrates a regulatory function of LTP1 in ethylene receptor signaling through the molecular association with RTE1. This study uncovers a novel function of Arabidopsis LTP1 in the regulation of ethylene response and signaling.</description><subject>Arabidopsis</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - metabolism</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Arabidopsis thaliana</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Ethylenes - metabolism</subject><subject>Flowers & plants</subject><subject>Gene Expression Regulation, Plant</subject><subject>Life Sciences</subject><subject>Mutation - genetics</subject><subject>Plant biology</subject><subject>Plant growth</subject><subject>Plant Pathology</subject><subject>Plant Sciences</subject><subject>Plants, Genetically Modified</subject><subject>Protein Binding</subject><subject>Protein expression</subject><subject>Protein Transport</subject><subject>Signal Transduction</subject><subject>Subcellular Fractions - metabolism</subject><subject>Transgenic plants</subject><issn>0167-4412</issn><issn>1573-5028</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkU1r3DAQhkVpabbb_oBeiqCX9OB29GHJOobQL1hICOlZaK3xVsEruZJ92H8fBaelFEqYwwzM874j8RLylsFHBqA_FcYYiAaYakB2vNHPyIa1WjQt8O452dSFbqRk_Iy8KuUOoKqEeknOuAajDYgNwRs8LKObUz7RYYn9HFKkaaAX2e2DT1MJhY5hCp7O2cUyYKZTTjOGSBk9391esw-0zjj_PI0YkWYsU4oFqYuelnCIbgzx8Jq8GNxY8M1j35IfXz7fXn5rdldfv19e7JpeQjs3CrgftObSKPQSOq961yqxB8EleAYOvDI923OlBjPIFoUEcKYD7mozKLbkfPWtb_y1YJntMZQex9FFTEuxrINOaehAPI1q0xkjWK0tef8PepeWXH-2Uhw0B14ptlJ9TqVkHOyUw9Hlk2VgH-Kya1y2pmIf4rK6at49Oi_7I_o_it_5VICvQKmreMD81-n_ut4Dq7mdmw</recordid><startdate>20160701</startdate><enddate>20160701</enddate><creator>Wang, Honglin</creator><creator>Sun, Yue</creator><creator>Chang, Jianhong</creator><creator>Zheng, Fangfang</creator><creator>Pei, Haixia</creator><creator>Yi, Yanjun</creator><creator>Chang, Caren</creator><creator>Dong, Chun-Hai</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20160701</creationdate><title>Regulatory function of Arabidopsis lipid transfer protein 1 (LTP1) in ethylene response and signaling</title><author>Wang, Honglin ; Sun, Yue ; Chang, Jianhong ; Zheng, Fangfang ; Pei, Haixia ; Yi, Yanjun ; Chang, Caren ; Dong, Chun-Hai</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-602df772496ed408d6ca563b03240d10a0d69c1b266f9f45e3400a9802a0a99e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Arabidopsis</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - metabolism</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>Arabidopsis thaliana</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Ethylenes - metabolism</topic><topic>Flowers & plants</topic><topic>Gene Expression Regulation, Plant</topic><topic>Life Sciences</topic><topic>Mutation - genetics</topic><topic>Plant biology</topic><topic>Plant growth</topic><topic>Plant Pathology</topic><topic>Plant Sciences</topic><topic>Plants, Genetically Modified</topic><topic>Protein Binding</topic><topic>Protein expression</topic><topic>Protein Transport</topic><topic>Signal Transduction</topic><topic>Subcellular Fractions - metabolism</topic><topic>Transgenic plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Honglin</creatorcontrib><creatorcontrib>Sun, Yue</creatorcontrib><creatorcontrib>Chang, Jianhong</creatorcontrib><creatorcontrib>Zheng, Fangfang</creatorcontrib><creatorcontrib>Pei, Haixia</creatorcontrib><creatorcontrib>Yi, Yanjun</creatorcontrib><creatorcontrib>Chang, Caren</creatorcontrib><creatorcontrib>Dong, Chun-Hai</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection (ProQuest)</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Honglin</au><au>Sun, Yue</au><au>Chang, Jianhong</au><au>Zheng, Fangfang</au><au>Pei, Haixia</au><au>Yi, Yanjun</au><au>Chang, Caren</au><au>Dong, Chun-Hai</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regulatory function of Arabidopsis lipid transfer protein 1 (LTP1) in ethylene response and signaling</atitle><jtitle>Plant molecular biology</jtitle><stitle>Plant Mol Biol</stitle><addtitle>Plant Mol Biol</addtitle><date>2016-07-01</date><risdate>2016</risdate><volume>91</volume><issue>4-5</issue><spage>471</spage><epage>484</epage><pages>471-484</pages><issn>0167-4412</issn><eissn>1573-5028</eissn><abstract>Ethylene as a gaseous plant hormone is directly involved in various processes during plant growth and development. Much is known regarding the ethylene receptors and regulatory factors in the ethylene signal transduction pathway. In
Arabidopsis thaliana
, REVERSION-TO-ETHYLENE SENSITIVITY1 (RTE1) can interact with and positively regulates the ethylene receptor ETHYLENE RESPONSE1 (ETR1). In this study we report the identification and characterization of an RTE1-interacting protein, a putative Arabidopsis lipid transfer protein 1 (LTP1) of unknown function. Through bimolecular fluorescence complementation, a direct molecular interaction between LTP1 and RTE1 was verified in planta. Analysis of an LTP1-GFP fusion in transgenic plants and plasmolysis experiments revealed that LTP1 is localized to the cytoplasm. Analysis of ethylene responses showed that the
ltp1
knockout is hypersensitive to 1-aminocyclopropanecarboxylic acid (ACC), while
LTP1
overexpression confers insensitivity. Analysis of double mutants
etr1
-
2 ltp1
and
rte1
-
3 ltp1
demonstrates a regulatory function of LTP1 in ethylene receptor signaling through the molecular association with RTE1. This study uncovers a novel function of Arabidopsis LTP1 in the regulation of ethylene response and signaling.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>27097903</pmid><doi>10.1007/s11103-016-0482-7</doi><tpages>14</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0167-4412 |
| ispartof | Plant molecular biology, 2016-07, Vol.91 (4-5), p.471-484 |
| issn | 0167-4412 1573-5028 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1808670803 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Arabidopsis Arabidopsis - genetics Arabidopsis - metabolism Arabidopsis Proteins - metabolism Arabidopsis thaliana Biochemistry Biomedical and Life Sciences Ethylenes - metabolism Flowers & plants Gene Expression Regulation, Plant Life Sciences Mutation - genetics Plant biology Plant growth Plant Pathology Plant Sciences Plants, Genetically Modified Protein Binding Protein expression Protein Transport Signal Transduction Subcellular Fractions - metabolism Transgenic plants |
| title | Regulatory function of Arabidopsis lipid transfer protein 1 (LTP1) in ethylene response and signaling |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-13T08%3A42%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Regulatory%20function%20of%20Arabidopsis%20lipid%20transfer%20protein%201%20(LTP1)%20in%20ethylene%20response%20and%20signaling&rft.jtitle=Plant%20molecular%20biology&rft.au=Wang,%20Honglin&rft.date=2016-07-01&rft.volume=91&rft.issue=4-5&rft.spage=471&rft.epage=484&rft.pages=471-484&rft.issn=0167-4412&rft.eissn=1573-5028&rft_id=info:doi/10.1007/s11103-016-0482-7&rft_dat=%3Cproquest_cross%3E4095046551%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1798207202&rft_id=info:pmid/27097903&rfr_iscdi=true |