Comprehensive panel of cross-reacting monoclonal antibodies for analysis of different immune cells and their distribution in the common marmoset (Callithrix jacchus)
Background Common marmosets are extensively used in immunological and pharmacological research, and the usage of methods such as flow cytometry gain increasing importance. Methods Using multicolor flow cytometry cross‐reactivity of monoclonal antibodies with cells of common marmosets was analyzed. F...
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| Veröffentlicht in: | Journal of medical primatology 2016-06, Vol.45 (3), p.139-146 |
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| container_title | Journal of medical primatology |
| container_volume | 45 |
| creator | Neumann, Berit Shi, Tingchuan Gan, Li Lin Klippert, Antonina Daskalaki, Maria Stolte-Leeb, Nicole Stahl-Hennig, Christiane |
| description | Background
Common marmosets are extensively used in immunological and pharmacological research, and the usage of methods such as flow cytometry gain increasing importance.
Methods
Using multicolor flow cytometry cross‐reactivity of monoclonal antibodies with cells of common marmosets was analyzed. Furthermore, frequencies of immune cells and immunological parameters were assessed in healthy common marmosets.
Results
A total of 97 clones of monoclonal antibodies raised against CD markers, chemokine receptors, and miscellaneous markers were tested. Additionally, baseline frequencies of different innate and adaptive immune cells as well as certain parameters, such as activation and memory T‐cell and B‐cell distribution, are provided.
Conclusion
Our study gives an extended overview of cross‐reactive antibodies for flow cytometric analysis of immune cells as well as baseline values for different immune parameters in healthy common marmosets. |
| doi_str_mv | 10.1111/jmp.12216 |
| format | Article |
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Common marmosets are extensively used in immunological and pharmacological research, and the usage of methods such as flow cytometry gain increasing importance.
Methods
Using multicolor flow cytometry cross‐reactivity of monoclonal antibodies with cells of common marmosets was analyzed. Furthermore, frequencies of immune cells and immunological parameters were assessed in healthy common marmosets.
Results
A total of 97 clones of monoclonal antibodies raised against CD markers, chemokine receptors, and miscellaneous markers were tested. Additionally, baseline frequencies of different innate and adaptive immune cells as well as certain parameters, such as activation and memory T‐cell and B‐cell distribution, are provided.
Conclusion
Our study gives an extended overview of cross‐reactive antibodies for flow cytometric analysis of immune cells as well as baseline values for different immune parameters in healthy common marmosets.</description><identifier>ISSN: 0047-2565</identifier><identifier>EISSN: 1600-0684</identifier><identifier>DOI: 10.1111/jmp.12216</identifier><identifier>PMID: 27221549</identifier><language>eng</language><publisher>Denmark: Blackwell Publishing Ltd</publisher><subject>Animals ; Antibodies, Monoclonal - immunology ; Callithrix - immunology ; Callithrix jacchus ; Cross Reactions ; Dendritic Cells - immunology ; Flow Cytometry ; Immunoglobulins ; Immunology ; immunophenotyping ; innate and adaptive immunity ; Leukocytes - immunology ; New World monkeys</subject><ispartof>Journal of medical primatology, 2016-06, Vol.45 (3), p.139-146</ispartof><rights>2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd</rights><rights>2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.</rights><rights>Copyright © 2016 John Wiley & Sons A/S</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4246-5d9b64390fd679042fdd99601ba0eaffc8bc58f08c8eaad1c1d3d0b3c74106b53</citedby><cites>FETCH-LOGICAL-c4246-5d9b64390fd679042fdd99601ba0eaffc8bc58f08c8eaad1c1d3d0b3c74106b53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjmp.12216$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjmp.12216$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27221549$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Neumann, Berit</creatorcontrib><creatorcontrib>Shi, Tingchuan</creatorcontrib><creatorcontrib>Gan, Li Lin</creatorcontrib><creatorcontrib>Klippert, Antonina</creatorcontrib><creatorcontrib>Daskalaki, Maria</creatorcontrib><creatorcontrib>Stolte-Leeb, Nicole</creatorcontrib><creatorcontrib>Stahl-Hennig, Christiane</creatorcontrib><title>Comprehensive panel of cross-reacting monoclonal antibodies for analysis of different immune cells and their distribution in the common marmoset (Callithrix jacchus)</title><title>Journal of medical primatology</title><addtitle>J Med Primatol</addtitle><description>Background
Common marmosets are extensively used in immunological and pharmacological research, and the usage of methods such as flow cytometry gain increasing importance.
Methods
Using multicolor flow cytometry cross‐reactivity of monoclonal antibodies with cells of common marmosets was analyzed. Furthermore, frequencies of immune cells and immunological parameters were assessed in healthy common marmosets.
Results
A total of 97 clones of monoclonal antibodies raised against CD markers, chemokine receptors, and miscellaneous markers were tested. Additionally, baseline frequencies of different innate and adaptive immune cells as well as certain parameters, such as activation and memory T‐cell and B‐cell distribution, are provided.
Conclusion
Our study gives an extended overview of cross‐reactive antibodies for flow cytometric analysis of immune cells as well as baseline values for different immune parameters in healthy common marmosets.</description><subject>Animals</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Callithrix - immunology</subject><subject>Callithrix jacchus</subject><subject>Cross Reactions</subject><subject>Dendritic Cells - immunology</subject><subject>Flow Cytometry</subject><subject>Immunoglobulins</subject><subject>Immunology</subject><subject>immunophenotyping</subject><subject>innate and adaptive immunity</subject><subject>Leukocytes - immunology</subject><subject>New World monkeys</subject><issn>0047-2565</issn><issn>1600-0684</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctu1DAUhiNERYfCghdAlti0i7R24jjxEk2hUA33IthZji-MB1-CnUDngXhPnJm2CySEN5at73zS-f-ieILgKcrnbOOGU1RViNwrFohAWELS4fvFAkLcllVDmsPiYUobCGGNKX5QHFZtphtMF8XvZXBDVGvlk_mpwMC9siBoIGJIqYyKi9H4b8AFH4QNnlvA_Wj6II1KQIeYn9xuk0nzkDRaq6j8CIxzk1dAKGtTRiQY18rEDKQxmn4aTfDA-PkXiOCyHTgeXUhqBMdLbq0Z19Fcgw0XYj2lk0fFgeY2qcc391Hx-eWLq-WrcvXu4vXy-aoUuMKkbCTtCa4p1JK0FOJKS0kpgajnUHGtRdeLptOwE53iXCKBZC1hX4sWI0j6pj4qjvfeIYYfk0ojcybNS-RYwpQY6mBHCO5q8n-0pajNPJmtz_5CN2GKObcdBSls6x11sqd20Uel2RBNTmXLEGRzzSzXzHY1Z_bpjXHqnZJ35G2vGTjbA7-MVdt_m9jlm_e3ynI_kStS13cTPH5npK3bhn15e8E-fvp69WF13jBa_wHrx8Py</recordid><startdate>201606</startdate><enddate>201606</enddate><creator>Neumann, Berit</creator><creator>Shi, Tingchuan</creator><creator>Gan, Li Lin</creator><creator>Klippert, Antonina</creator><creator>Daskalaki, Maria</creator><creator>Stolte-Leeb, Nicole</creator><creator>Stahl-Hennig, Christiane</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7TK</scope><scope>K9.</scope><scope>7X8</scope><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>201606</creationdate><title>Comprehensive panel of cross-reacting monoclonal antibodies for analysis of different immune cells and their distribution in the common marmoset (Callithrix jacchus)</title><author>Neumann, Berit ; Shi, Tingchuan ; Gan, Li Lin ; Klippert, Antonina ; Daskalaki, Maria ; Stolte-Leeb, Nicole ; Stahl-Hennig, Christiane</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4246-5d9b64390fd679042fdd99601ba0eaffc8bc58f08c8eaad1c1d3d0b3c74106b53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Callithrix - immunology</topic><topic>Callithrix jacchus</topic><topic>Cross Reactions</topic><topic>Dendritic Cells - immunology</topic><topic>Flow Cytometry</topic><topic>Immunoglobulins</topic><topic>Immunology</topic><topic>immunophenotyping</topic><topic>innate and adaptive immunity</topic><topic>Leukocytes - immunology</topic><topic>New World monkeys</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Neumann, Berit</creatorcontrib><creatorcontrib>Shi, Tingchuan</creatorcontrib><creatorcontrib>Gan, Li Lin</creatorcontrib><creatorcontrib>Klippert, Antonina</creatorcontrib><creatorcontrib>Daskalaki, Maria</creatorcontrib><creatorcontrib>Stolte-Leeb, Nicole</creatorcontrib><creatorcontrib>Stahl-Hennig, Christiane</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Journal of medical primatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Neumann, Berit</au><au>Shi, Tingchuan</au><au>Gan, Li Lin</au><au>Klippert, Antonina</au><au>Daskalaki, Maria</au><au>Stolte-Leeb, Nicole</au><au>Stahl-Hennig, Christiane</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comprehensive panel of cross-reacting monoclonal antibodies for analysis of different immune cells and their distribution in the common marmoset (Callithrix jacchus)</atitle><jtitle>Journal of medical primatology</jtitle><addtitle>J Med Primatol</addtitle><date>2016-06</date><risdate>2016</risdate><volume>45</volume><issue>3</issue><spage>139</spage><epage>146</epage><pages>139-146</pages><issn>0047-2565</issn><eissn>1600-0684</eissn><abstract>Background
Common marmosets are extensively used in immunological and pharmacological research, and the usage of methods such as flow cytometry gain increasing importance.
Methods
Using multicolor flow cytometry cross‐reactivity of monoclonal antibodies with cells of common marmosets was analyzed. Furthermore, frequencies of immune cells and immunological parameters were assessed in healthy common marmosets.
Results
A total of 97 clones of monoclonal antibodies raised against CD markers, chemokine receptors, and miscellaneous markers were tested. Additionally, baseline frequencies of different innate and adaptive immune cells as well as certain parameters, such as activation and memory T‐cell and B‐cell distribution, are provided.
Conclusion
Our study gives an extended overview of cross‐reactive antibodies for flow cytometric analysis of immune cells as well as baseline values for different immune parameters in healthy common marmosets.</abstract><cop>Denmark</cop><pub>Blackwell Publishing Ltd</pub><pmid>27221549</pmid><doi>10.1111/jmp.12216</doi><tpages>8</tpages></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Antibodies, Monoclonal - immunology Callithrix - immunology Callithrix jacchus Cross Reactions Dendritic Cells - immunology Flow Cytometry Immunoglobulins Immunology immunophenotyping innate and adaptive immunity Leukocytes - immunology New World monkeys |
| title | Comprehensive panel of cross-reacting monoclonal antibodies for analysis of different immune cells and their distribution in the common marmoset (Callithrix jacchus) |
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