Shikonin Inhibits Inflammatory Cytokine Production in Human Periodontal Ligament Cells
Shikonin, which is derived from Lithospermum erythrorhizon , a herb used in traditional medicine, has long been considered to be a useful treatment for various diseases in traditional oriental medicine. Shikonin has recently been reported to have several pharmacological properties, e.g., it has anti...
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| Veröffentlicht in: | Inflammation 2016-06, Vol.39 (3), p.1124-1129 |
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| creator | Shindo, Satoru Hosokawa, Yoshitaka Hosokawa, Ikuko Ozaki, Kazumi Matsuo, Takashi |
| description | Shikonin, which is derived from
Lithospermum erythrorhizon
, a herb used in traditional medicine, has long been considered to be a useful treatment for various diseases in traditional oriental medicine. Shikonin has recently been reported to have several pharmacological properties, e.g., it has anti-microbial, anti-tumor, and anti-inflammatory effects. The aim of this study was to examine whether shikonin is able to influence the production of interleukin (IL)-6, IL-8, and/or chemokine C-C motif ligand (CCL)20, which contribute to the pathogenesis of periodontal disease, in human periodontal ligament cells (HPDLC). The production levels of IL-6, IL-8, and CCL20 in HPDLC were determined using an ELISA. Western blot analysis was used to detect nuclear factor kappa B (NF-κB) pathway activation in HPDLC. Shikonin prevented IL-1β- or tumor necrosis factor (TNF)-α-mediated IL-6, IL-8, and CCL20 production in HPDLC. Moreover, we found that shikonin suppressed the phosphorylation and degradation of inhibitor of kappa B-alpha (IκB-α) in IL-1β- or TNF-α-stimulated HPDLC. These findings suggest that shikonin could have direct beneficial effects against periodontal disease by reducing IL-6, IL-8, and CCL20 production in periodontal lesions. |
| doi_str_mv | 10.1007/s10753-016-0344-0 |
| format | Article |
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Lithospermum erythrorhizon
, a herb used in traditional medicine, has long been considered to be a useful treatment for various diseases in traditional oriental medicine. Shikonin has recently been reported to have several pharmacological properties, e.g., it has anti-microbial, anti-tumor, and anti-inflammatory effects. The aim of this study was to examine whether shikonin is able to influence the production of interleukin (IL)-6, IL-8, and/or chemokine C-C motif ligand (CCL)20, which contribute to the pathogenesis of periodontal disease, in human periodontal ligament cells (HPDLC). The production levels of IL-6, IL-8, and CCL20 in HPDLC were determined using an ELISA. Western blot analysis was used to detect nuclear factor kappa B (NF-κB) pathway activation in HPDLC. Shikonin prevented IL-1β- or tumor necrosis factor (TNF)-α-mediated IL-6, IL-8, and CCL20 production in HPDLC. Moreover, we found that shikonin suppressed the phosphorylation and degradation of inhibitor of kappa B-alpha (IκB-α) in IL-1β- or TNF-α-stimulated HPDLC. These findings suggest that shikonin could have direct beneficial effects against periodontal disease by reducing IL-6, IL-8, and CCL20 production in periodontal lesions.</description><identifier>ISSN: 0360-3997</identifier><identifier>EISSN: 1573-2576</identifier><identifier>DOI: 10.1007/s10753-016-0344-0</identifier><identifier>PMID: 27072015</identifier><identifier>CODEN: INFLD4</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Anti-Inflammatory Agents, Non-Steroidal - pharmacology ; Biomedical and Life Sciences ; Biomedicine ; Cells, Cultured ; Chemokine CCL20 - biosynthesis ; Chemokine CCL20 - drug effects ; Cytokines - biosynthesis ; Cytokines - drug effects ; Humans ; Immunology ; Inflammation ; Interleukin-6 - antagonists & inhibitors ; Interleukin-6 - biosynthesis ; Interleukin-8 - biosynthesis ; Interleukin-8 - drug effects ; Internal Medicine ; Lithospermum erythrorhizon ; Naphthoquinones - pharmacology ; Original Article ; Pathology ; Periodontal Diseases - drug therapy ; Periodontal Diseases - pathology ; Periodontal Ligament - cytology ; Periodontal Ligament - drug effects ; Pharmacology/Toxicology ; Rheumatology</subject><ispartof>Inflammation, 2016-06, Vol.39 (3), p.1124-1129</ispartof><rights>Springer Science+Business Media New York 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c467t-9391576122bc4624821b42c80b2bb375cb284a537b352e59725d1b17ecd198c23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10753-016-0344-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10753-016-0344-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51298</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27072015$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shindo, Satoru</creatorcontrib><creatorcontrib>Hosokawa, Yoshitaka</creatorcontrib><creatorcontrib>Hosokawa, Ikuko</creatorcontrib><creatorcontrib>Ozaki, Kazumi</creatorcontrib><creatorcontrib>Matsuo, Takashi</creatorcontrib><title>Shikonin Inhibits Inflammatory Cytokine Production in Human Periodontal Ligament Cells</title><title>Inflammation</title><addtitle>Inflammation</addtitle><addtitle>Inflammation</addtitle><description>Shikonin, which is derived from
Lithospermum erythrorhizon
, a herb used in traditional medicine, has long been considered to be a useful treatment for various diseases in traditional oriental medicine. Shikonin has recently been reported to have several pharmacological properties, e.g., it has anti-microbial, anti-tumor, and anti-inflammatory effects. The aim of this study was to examine whether shikonin is able to influence the production of interleukin (IL)-6, IL-8, and/or chemokine C-C motif ligand (CCL)20, which contribute to the pathogenesis of periodontal disease, in human periodontal ligament cells (HPDLC). The production levels of IL-6, IL-8, and CCL20 in HPDLC were determined using an ELISA. Western blot analysis was used to detect nuclear factor kappa B (NF-κB) pathway activation in HPDLC. Shikonin prevented IL-1β- or tumor necrosis factor (TNF)-α-mediated IL-6, IL-8, and CCL20 production in HPDLC. Moreover, we found that shikonin suppressed the phosphorylation and degradation of inhibitor of kappa B-alpha (IκB-α) in IL-1β- or TNF-α-stimulated HPDLC. These findings suggest that shikonin could have direct beneficial effects against periodontal disease by reducing IL-6, IL-8, and CCL20 production in periodontal lesions.</description><subject>Anti-Inflammatory Agents, Non-Steroidal - pharmacology</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cells, Cultured</subject><subject>Chemokine CCL20 - biosynthesis</subject><subject>Chemokine CCL20 - drug effects</subject><subject>Cytokines - biosynthesis</subject><subject>Cytokines - drug effects</subject><subject>Humans</subject><subject>Immunology</subject><subject>Inflammation</subject><subject>Interleukin-6 - antagonists & inhibitors</subject><subject>Interleukin-6 - biosynthesis</subject><subject>Interleukin-8 - biosynthesis</subject><subject>Interleukin-8 - drug effects</subject><subject>Internal Medicine</subject><subject>Lithospermum erythrorhizon</subject><subject>Naphthoquinones - pharmacology</subject><subject>Original Article</subject><subject>Pathology</subject><subject>Periodontal Diseases - drug therapy</subject><subject>Periodontal Diseases - pathology</subject><subject>Periodontal Ligament - cytology</subject><subject>Periodontal Ligament - drug effects</subject><subject>Pharmacology/Toxicology</subject><subject>Rheumatology</subject><issn>0360-3997</issn><issn>1573-2576</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNqFkU1LxDAQhoMouq7-AC9S8OKlOpM0SXuUxS9YUPDjWpI2q9E20aQ97L83y6qIIF6SMHnyzoSHkAOEEwSQpxFBcpYDihxYUeSwQSbIJcspl2KTTIAJyFlVyR2yG-MLAJRVybbJDpUgKSCfkMe7Z_vqnXXZtXu22g4xHRad6ns1-LDMZsvBv1pnstvg27EZrHdZgq_GXrns1gTrW-8G1WVz-6R644ZsZrou7pGtheqi2f_cp-Th4vx-dpXPby6vZ2fzvCmEHPKKVWlegZTqVKBFSVEXtClBU62Z5I2mZaE4k5pxanglKW9RozRNi1XZUDYlx-vct-DfRxOHurexSRMoZ_wYayyhFAIQ8H9UVpSlBnKVevQLffFjcOkjKwoFClEUicI11QQfYzCL-i3YXoVljVCv_NRrP3XyU6_8pGVKDj-TR92b9vvFl5AE0DUQ05V7MuFH6z9TPwDFUJiC</recordid><startdate>20160601</startdate><enddate>20160601</enddate><creator>Shindo, Satoru</creator><creator>Hosokawa, Yoshitaka</creator><creator>Hosokawa, Ikuko</creator><creator>Ozaki, Kazumi</creator><creator>Matsuo, Takashi</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20160601</creationdate><title>Shikonin Inhibits Inflammatory Cytokine Production in Human Periodontal Ligament Cells</title><author>Shindo, Satoru ; Hosokawa, Yoshitaka ; Hosokawa, Ikuko ; Ozaki, Kazumi ; Matsuo, Takashi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c467t-9391576122bc4624821b42c80b2bb375cb284a537b352e59725d1b17ecd198c23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Anti-Inflammatory Agents, Non-Steroidal - pharmacology</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cells, Cultured</topic><topic>Chemokine CCL20 - biosynthesis</topic><topic>Chemokine CCL20 - drug effects</topic><topic>Cytokines - biosynthesis</topic><topic>Cytokines - drug effects</topic><topic>Humans</topic><topic>Immunology</topic><topic>Inflammation</topic><topic>Interleukin-6 - antagonists & inhibitors</topic><topic>Interleukin-6 - biosynthesis</topic><topic>Interleukin-8 - biosynthesis</topic><topic>Interleukin-8 - drug effects</topic><topic>Internal Medicine</topic><topic>Lithospermum erythrorhizon</topic><topic>Naphthoquinones - pharmacology</topic><topic>Original Article</topic><topic>Pathology</topic><topic>Periodontal Diseases - drug therapy</topic><topic>Periodontal Diseases - pathology</topic><topic>Periodontal Ligament - cytology</topic><topic>Periodontal Ligament - drug effects</topic><topic>Pharmacology/Toxicology</topic><topic>Rheumatology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shindo, Satoru</creatorcontrib><creatorcontrib>Hosokawa, Yoshitaka</creatorcontrib><creatorcontrib>Hosokawa, Ikuko</creatorcontrib><creatorcontrib>Ozaki, Kazumi</creatorcontrib><creatorcontrib>Matsuo, Takashi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Inflammation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shindo, Satoru</au><au>Hosokawa, Yoshitaka</au><au>Hosokawa, Ikuko</au><au>Ozaki, Kazumi</au><au>Matsuo, Takashi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Shikonin Inhibits Inflammatory Cytokine Production in Human Periodontal Ligament Cells</atitle><jtitle>Inflammation</jtitle><stitle>Inflammation</stitle><addtitle>Inflammation</addtitle><date>2016-06-01</date><risdate>2016</risdate><volume>39</volume><issue>3</issue><spage>1124</spage><epage>1129</epage><pages>1124-1129</pages><issn>0360-3997</issn><eissn>1573-2576</eissn><coden>INFLD4</coden><abstract>Shikonin, which is derived from
Lithospermum erythrorhizon
, a herb used in traditional medicine, has long been considered to be a useful treatment for various diseases in traditional oriental medicine. Shikonin has recently been reported to have several pharmacological properties, e.g., it has anti-microbial, anti-tumor, and anti-inflammatory effects. The aim of this study was to examine whether shikonin is able to influence the production of interleukin (IL)-6, IL-8, and/or chemokine C-C motif ligand (CCL)20, which contribute to the pathogenesis of periodontal disease, in human periodontal ligament cells (HPDLC). The production levels of IL-6, IL-8, and CCL20 in HPDLC were determined using an ELISA. Western blot analysis was used to detect nuclear factor kappa B (NF-κB) pathway activation in HPDLC. Shikonin prevented IL-1β- or tumor necrosis factor (TNF)-α-mediated IL-6, IL-8, and CCL20 production in HPDLC. Moreover, we found that shikonin suppressed the phosphorylation and degradation of inhibitor of kappa B-alpha (IκB-α) in IL-1β- or TNF-α-stimulated HPDLC. These findings suggest that shikonin could have direct beneficial effects against periodontal disease by reducing IL-6, IL-8, and CCL20 production in periodontal lesions.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>27072015</pmid><doi>10.1007/s10753-016-0344-0</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Anti-Inflammatory Agents, Non-Steroidal - pharmacology Biomedical and Life Sciences Biomedicine Cells, Cultured Chemokine CCL20 - biosynthesis Chemokine CCL20 - drug effects Cytokines - biosynthesis Cytokines - drug effects Humans Immunology Inflammation Interleukin-6 - antagonists & inhibitors Interleukin-6 - biosynthesis Interleukin-8 - biosynthesis Interleukin-8 - drug effects Internal Medicine Lithospermum erythrorhizon Naphthoquinones - pharmacology Original Article Pathology Periodontal Diseases - drug therapy Periodontal Diseases - pathology Periodontal Ligament - cytology Periodontal Ligament - drug effects Pharmacology/Toxicology Rheumatology |
| title | Shikonin Inhibits Inflammatory Cytokine Production in Human Periodontal Ligament Cells |
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