A rapid, highly sensitive and culture-free detection of pathogens from whole blood by removal of white blood cells using immuno-magnetic beads

Using anti-human CD45 antibody coated beads, we show a 98% reduction of WBCs from spiked blood samples in 1h, thereby enriching it for pathogens. This enrichment allowed the detection of

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Bibliographische Detailangaben
Veröffentlicht in:	Journal of microbiological methods 2016-08, Vol.127, p.59-61
Hauptverfasser:	Vutukuru, Manjula Ramya, Sharma, Divya Khandige, MS, Ragavendar, Mitra, Nivedita
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical sensitivity Bacteria - classification Bacteria - genetics Bacteria - immunology Bacteria - isolation & purification Blood - microbiology Blood stream infections CD45 Culture-free detection Escherichia coli Escherichia coli - genetics Escherichia coli - immunology Escherichia coli - isolation & purification Escherichia coli Infections - diagnosis Healthcare acquired infections Humans Immunomagnetic Separation - methods Leukocyte Common Antigens - immunology Leukocytes Limit of Detection Low pathogen load Molecular diagnostics and culture-free detection of pathogens from whole blood Pathogen enrichment Polymerase Chain Reaction - methods Quantitative PCR (qPCR) Sensitivity and Specificity Sepsis
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Beschreibung
Zusammenfassung:	Using anti-human CD45 antibody coated beads, we show a 98% reduction of WBCs from spiked blood samples in 1h, thereby enriching it for pathogens. This enrichment allowed the detection of
ISSN:	0167-7012 1872-8359
DOI:	10.1016/j.mimet.2016.05.018