Effect of Different Levels of Silymarin and Caproic Acid on Storage of Ram Semen in Liquid Form
Contents Two experiments were designed to evaluate the effect of silymarin on stored spermatozoa using four rams. In experiment 1, silymarin was evaluated as a supplement for Tris–glucose extender. Semen samples (n = 20) were diluted with extender containing 0, 50, 100, 150 and 200 μg/ml silymarin a...
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| Veröffentlicht in: | Reproduction in domestic animals 2016-08, Vol.51 (4), p.569-574 |
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| creator | Roostaei-Ali Mehr, M Parisoush, P |
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Two experiments were designed to evaluate the effect of silymarin on stored spermatozoa using four rams. In experiment 1, silymarin was evaluated as a supplement for Tris–glucose extender. Semen samples (n = 20) were diluted with extender containing 0, 50, 100, 150 and 200 μg/ml silymarin and incubated at 5°C for 72 h. Membrane integrity, acrosome integrity, sperm viability and motility were evaluated at 72 h. Concentration of malondialdehyde (MDA) was determined after 48 h. Membrane integrity was higher in 100 μg/ml silymarin (65.2%) than control group (43.2%, p |
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Two experiments were designed to evaluate the effect of silymarin on stored spermatozoa using four rams. In experiment 1, silymarin was evaluated as a supplement for Tris–glucose extender. Semen samples (n = 20) were diluted with extender containing 0, 50, 100, 150 and 200 μg/ml silymarin and incubated at 5°C for 72 h. Membrane integrity, acrosome integrity, sperm viability and motility were evaluated at 72 h. Concentration of malondialdehyde (MDA) was determined after 48 h. Membrane integrity was higher in 100 μg/ml silymarin (65.2%) than control group (43.2%, p < 0.05). Acrosome integrity was highest in 100 μg/ml silymarin (71.3%, p < 0.05). Progressive motility was higher in 100 (58.5%), 150 (60.62%) and 200 μg/ml silymarin (54.7%) than control group (30.7%, p < 0.05). The highest MDA concentration was observed in control group (400 mm/10 × 106 sperm; p < 0.05). The goal of experiment 2 was to determine the interaction between silymarin and caproic acid on ram stored sperm. Ejaculates (n = 20) were diluted by Tris–glucose extender, added 0 (S−) or 100 μg/ml (S+) silymarin and 0 (C−) or 0.3125% (C+) caproic acid, and thereafter, aliquots were incubated at 5°C for 72 h. Membrane integrity was lower in C−S− (57.6%) than C−S+ (73.2%), C+S− (80.2%) and C+S+ (72.1%, p > 0.05). The highest sperm viability and acrosome integrity were observed in C+S‐ (82.4 and 80.1%, respectively; p < 0.05). There was no difference between C‐S+ and C+S+ on sperm viability and membrane integrity, progressive motility and MDA concentration (p > 0.05). Therefore, the supplementation of extender with silymarin and caproic acid improved sperm quality and caproic acid was superior to caproic acid plus silymarin.</description><identifier>ISSN: 0936-6768</identifier><identifier>EISSN: 1439-0531</identifier><identifier>DOI: 10.1111/rda.12721</identifier><identifier>PMID: 27321767</identifier><language>eng</language><publisher>Germany: Blackwell Publishing Ltd</publisher><subject>Acids ; Animal reproduction ; Animals ; Body fluids ; Caproates - administration & dosage ; Caproates - pharmacology ; Cold Temperature ; Dilution ; Dose-Response Relationship, Drug ; Glucose ; Hexanoic acid ; Integrity ; Lipid Peroxidation - drug effects ; Male ; Malondialdehyde ; Motility ; Plant Extracts - administration & dosage ; Plant Extracts - chemistry ; Plant Extracts - pharmacology ; Rams ; Semen ; Semen - physiology ; Semen Preservation - methods ; Semen Preservation - veterinary ; Sheep ; Sheep - physiology ; Silymarin ; Silymarin - chemistry ; Sperm ; Sperm Motility - drug effects ; Spermatozoa ; Supplements ; Viability</subject><ispartof>Reproduction in domestic animals, 2016-08, Vol.51 (4), p.569-574</ispartof><rights>2016 Blackwell Verlag GmbH</rights><rights>2016 Blackwell Verlag GmbH.</rights><rights>Copyright © 2016 Blackwell Verlag GmbH</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4521-e8dfd6f14dcd8dfbd0f550bf5ff7fee38d842ef9d1c9705c1d70f5cda1d691c53</citedby><cites>FETCH-LOGICAL-c4521-e8dfd6f14dcd8dfbd0f550bf5ff7fee38d842ef9d1c9705c1d70f5cda1d691c53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Frda.12721$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Frda.12721$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,777,781,1412,27905,27906,45555,45556</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27321767$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Roostaei-Ali Mehr, M</creatorcontrib><creatorcontrib>Parisoush, P</creatorcontrib><title>Effect of Different Levels of Silymarin and Caproic Acid on Storage of Ram Semen in Liquid Form</title><title>Reproduction in domestic animals</title><addtitle>Reprod Dom Anim</addtitle><description>Contents
Two experiments were designed to evaluate the effect of silymarin on stored spermatozoa using four rams. In experiment 1, silymarin was evaluated as a supplement for Tris–glucose extender. Semen samples (n = 20) were diluted with extender containing 0, 50, 100, 150 and 200 μg/ml silymarin and incubated at 5°C for 72 h. Membrane integrity, acrosome integrity, sperm viability and motility were evaluated at 72 h. Concentration of malondialdehyde (MDA) was determined after 48 h. Membrane integrity was higher in 100 μg/ml silymarin (65.2%) than control group (43.2%, p < 0.05). Acrosome integrity was highest in 100 μg/ml silymarin (71.3%, p < 0.05). Progressive motility was higher in 100 (58.5%), 150 (60.62%) and 200 μg/ml silymarin (54.7%) than control group (30.7%, p < 0.05). The highest MDA concentration was observed in control group (400 mm/10 × 106 sperm; p < 0.05). The goal of experiment 2 was to determine the interaction between silymarin and caproic acid on ram stored sperm. Ejaculates (n = 20) were diluted by Tris–glucose extender, added 0 (S−) or 100 μg/ml (S+) silymarin and 0 (C−) or 0.3125% (C+) caproic acid, and thereafter, aliquots were incubated at 5°C for 72 h. Membrane integrity was lower in C−S− (57.6%) than C−S+ (73.2%), C+S− (80.2%) and C+S+ (72.1%, p > 0.05). The highest sperm viability and acrosome integrity were observed in C+S‐ (82.4 and 80.1%, respectively; p < 0.05). There was no difference between C‐S+ and C+S+ on sperm viability and membrane integrity, progressive motility and MDA concentration (p > 0.05). Therefore, the supplementation of extender with silymarin and caproic acid improved sperm quality and caproic acid was superior to caproic acid plus silymarin.</description><subject>Acids</subject><subject>Animal reproduction</subject><subject>Animals</subject><subject>Body fluids</subject><subject>Caproates - administration & dosage</subject><subject>Caproates - pharmacology</subject><subject>Cold Temperature</subject><subject>Dilution</subject><subject>Dose-Response Relationship, Drug</subject><subject>Glucose</subject><subject>Hexanoic acid</subject><subject>Integrity</subject><subject>Lipid Peroxidation - drug effects</subject><subject>Male</subject><subject>Malondialdehyde</subject><subject>Motility</subject><subject>Plant Extracts - administration & dosage</subject><subject>Plant Extracts - chemistry</subject><subject>Plant Extracts - pharmacology</subject><subject>Rams</subject><subject>Semen</subject><subject>Semen - physiology</subject><subject>Semen Preservation - methods</subject><subject>Semen Preservation - veterinary</subject><subject>Sheep</subject><subject>Sheep - physiology</subject><subject>Silymarin</subject><subject>Silymarin - chemistry</subject><subject>Sperm</subject><subject>Sperm Motility - drug effects</subject><subject>Spermatozoa</subject><subject>Supplements</subject><subject>Viability</subject><issn>0936-6768</issn><issn>1439-0531</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0U1vFCEYB3BiNHatHvwChsRLPUwLDC8zx81ut5psatJtqzfCwoOhzksLM-p-exm37cGkRi4Q8nv-4eFB6C0lxzSvk-jMMWWK0WdoRnlZF0SU9DmakbqUhVSyOkCvUrohhIpKqZfogKmSUSXVDOlT78EOuPd4GfIxQjfgNfyAJk13m9DsWhNDh03n8MLcxj5YPLfB4b7Dm6GP5htM8MK0eAMtdDjbdbgbs1j1sX2NXnjTJHhzvx-iq9Xp5eJjsf589mkxXxeWC0YLqJx30lPurMvHrSNeCLL1wnvlAcrKVZyBrx21tSLCUqeysM5QJ2tqRXmIjva5-YV3I6RBtyFZaBrTQT8mTStSSSFFyf-HMsWZICTT93_Rm36MXW5EM0KrWkpa_1NNWbmRik_qw17Z2KcUwevbGPLf7jQlehqjzmPUf8aY7bv7xHHbgnuUD3PL4GQPfoYGdk8n6Yvl_CGy2FeENMCvxwoTv-ucp4T-cn6mCV99vV5uuL4sfwPgO7M_</recordid><startdate>201608</startdate><enddate>201608</enddate><creator>Roostaei-Ali Mehr, M</creator><creator>Parisoush, P</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201608</creationdate><title>Effect of Different Levels of Silymarin and Caproic Acid on Storage of Ram Semen in Liquid Form</title><author>Roostaei-Ali Mehr, M ; Parisoush, P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4521-e8dfd6f14dcd8dfbd0f550bf5ff7fee38d842ef9d1c9705c1d70f5cda1d691c53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Acids</topic><topic>Animal reproduction</topic><topic>Animals</topic><topic>Body fluids</topic><topic>Caproates - administration & dosage</topic><topic>Caproates - pharmacology</topic><topic>Cold Temperature</topic><topic>Dilution</topic><topic>Dose-Response Relationship, Drug</topic><topic>Glucose</topic><topic>Hexanoic acid</topic><topic>Integrity</topic><topic>Lipid Peroxidation - drug effects</topic><topic>Male</topic><topic>Malondialdehyde</topic><topic>Motility</topic><topic>Plant Extracts - administration & dosage</topic><topic>Plant Extracts - chemistry</topic><topic>Plant Extracts - pharmacology</topic><topic>Rams</topic><topic>Semen</topic><topic>Semen - physiology</topic><topic>Semen Preservation - methods</topic><topic>Semen Preservation - veterinary</topic><topic>Sheep</topic><topic>Sheep - physiology</topic><topic>Silymarin</topic><topic>Silymarin - chemistry</topic><topic>Sperm</topic><topic>Sperm Motility - drug effects</topic><topic>Spermatozoa</topic><topic>Supplements</topic><topic>Viability</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Roostaei-Ali Mehr, M</creatorcontrib><creatorcontrib>Parisoush, P</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Reproduction in domestic animals</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Roostaei-Ali Mehr, M</au><au>Parisoush, P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Different Levels of Silymarin and Caproic Acid on Storage of Ram Semen in Liquid Form</atitle><jtitle>Reproduction in domestic animals</jtitle><addtitle>Reprod Dom Anim</addtitle><date>2016-08</date><risdate>2016</risdate><volume>51</volume><issue>4</issue><spage>569</spage><epage>574</epage><pages>569-574</pages><issn>0936-6768</issn><eissn>1439-0531</eissn><abstract>Contents
Two experiments were designed to evaluate the effect of silymarin on stored spermatozoa using four rams. In experiment 1, silymarin was evaluated as a supplement for Tris–glucose extender. Semen samples (n = 20) were diluted with extender containing 0, 50, 100, 150 and 200 μg/ml silymarin and incubated at 5°C for 72 h. Membrane integrity, acrosome integrity, sperm viability and motility were evaluated at 72 h. Concentration of malondialdehyde (MDA) was determined after 48 h. Membrane integrity was higher in 100 μg/ml silymarin (65.2%) than control group (43.2%, p < 0.05). Acrosome integrity was highest in 100 μg/ml silymarin (71.3%, p < 0.05). Progressive motility was higher in 100 (58.5%), 150 (60.62%) and 200 μg/ml silymarin (54.7%) than control group (30.7%, p < 0.05). The highest MDA concentration was observed in control group (400 mm/10 × 106 sperm; p < 0.05). The goal of experiment 2 was to determine the interaction between silymarin and caproic acid on ram stored sperm. Ejaculates (n = 20) were diluted by Tris–glucose extender, added 0 (S−) or 100 μg/ml (S+) silymarin and 0 (C−) or 0.3125% (C+) caproic acid, and thereafter, aliquots were incubated at 5°C for 72 h. Membrane integrity was lower in C−S− (57.6%) than C−S+ (73.2%), C+S− (80.2%) and C+S+ (72.1%, p > 0.05). The highest sperm viability and acrosome integrity were observed in C+S‐ (82.4 and 80.1%, respectively; p < 0.05). There was no difference between C‐S+ and C+S+ on sperm viability and membrane integrity, progressive motility and MDA concentration (p > 0.05). Therefore, the supplementation of extender with silymarin and caproic acid improved sperm quality and caproic acid was superior to caproic acid plus silymarin.</abstract><cop>Germany</cop><pub>Blackwell Publishing Ltd</pub><pmid>27321767</pmid><doi>10.1111/rda.12721</doi><tpages>6</tpages></addata></record> |
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| ispartof | Reproduction in domestic animals, 2016-08, Vol.51 (4), p.569-574 |
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| source | Wiley Online Library - AutoHoldings Journals; MEDLINE |
| subjects | Acids Animal reproduction Animals Body fluids Caproates - administration & dosage Caproates - pharmacology Cold Temperature Dilution Dose-Response Relationship, Drug Glucose Hexanoic acid Integrity Lipid Peroxidation - drug effects Male Malondialdehyde Motility Plant Extracts - administration & dosage Plant Extracts - chemistry Plant Extracts - pharmacology Rams Semen Semen - physiology Semen Preservation - methods Semen Preservation - veterinary Sheep Sheep - physiology Silymarin Silymarin - chemistry Sperm Sperm Motility - drug effects Spermatozoa Supplements Viability |
| title | Effect of Different Levels of Silymarin and Caproic Acid on Storage of Ram Semen in Liquid Form |
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