Evaluation of therapeutic effects of natural killer (NK) cell‐based immunotherapy in mice using in vivo apoptosis bioimaging with a caspase‐3 sensor
ABSTRACT Natural killer (NK) cell‐based immunotherapy is a promising strategy for cancer treatment, and caspase‐3 is an important effector molecule in NK cell‐mediated apoptosis in cancers. Here, we evaluated the antitumor effects of NK cell‐based immunotherapy by serial noninvasive imaging of apopt...
Gespeichert in:
| Veröffentlicht in: | The FASEB journal 2014-07, Vol.28 (7), p.2932-2941 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 2941 |
|---|---|
| container_issue | 7 |
| container_start_page | 2932 |
| container_title | The FASEB journal |
| container_volume | 28 |
| creator | Lee, Ho Won Singh, Thoudam Debraj Lee, Sang‐Woo Ha, Jeoung‐Hee Rehemtulla, Alnawaz Ahn, Byeong‐Cheol Jeon, Young Hyun Lee, Jaetae |
| description | ABSTRACT
Natural killer (NK) cell‐based immunotherapy is a promising strategy for cancer treatment, and caspase‐3 is an important effector molecule in NK cell‐mediated apoptosis in cancers. Here, we evaluated the antitumor effects of NK cell‐based immunotherapy by serial noninvasive imaging of apoptosis using a caspase‐3 sensor in mice with human glioma xenografts. Human glioma cells expressing both a caspase‐3 sensor as a surrogate marker for caspase‐3 activation and Renilla luciferase (Rluc) as a surrogate marker for cell viability were established and referred to as D54‐CR cells. Human NK92 cells were used as effector cells. Treatment with NK92 cells resulted in a time‐ and effector number‐dependent increase in bioluminescence imaging (BLI) activity of the caspase‐3 sensor in D54‐CR cells in vitro. Caspase‐3 activation by NK92 treatment was blocked by Z‐VAD treatment in D54‐CR cells. Transfusion of NK92 cells induced an increase of the BLI signal by caspase‐3 activation in a dose‐ and time‐dependent manner in D54‐CR tumor‐bearing mice but not in PBS‐treated mice. Accordingly, sequential BLI with the Rluc reporter gene revealed marked retardation of tumor growth in the NK92‐treatment group but not in the PBS‐treatment group. These data suggest that noninvasive imaging of apoptosis with a caspase‐3 sensor can be used as an effective tool for evaluation of therapeutic efficacy as well as for optimization of NK cell‐based immunotherapy.—Lee, H. W., Singh, T. D., Lee, S.‐W., Ha, J.‐H., Rehemtulla, A., Ahn, B.‐C., Jeon, Y.‐H., Lee, J. Evaluation of therapeutic effects of natural killer (NK) cell‐based immunotherapy in mice using in vivo apoptosis bioimaging with a caspase‐3 sensor. FASEB J. 28, 2932–2941 (2014). www.fasebj.org |
| doi_str_mv | 10.1096/fj.13-243014 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1808653837</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1549636038</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4114-b021cc34e4326886619b3331892c93f1ab748c4ef22523d9b93e8cd86b6d1b683</originalsourceid><addsrcrecordid>eNqFkc1u1DAURi0EokNhxxp5WSRSfH09HmcJVYe_ChbA2rIdp_WQxKmdTDU7HoElz8eTkCgDS1hZ1_fcI336CHkK7BxYKV_Wu3PAggtkIO6RFayRFVJJdp-smCp5ISWqE_Io5x1jDBjIh-SEiw1KAbgiPy_3phnNEGJHY02HG59M78chOOrr2rshz9-dGcZkGvotNI1P9Ozjh-fU-ab59f2HNdlXNLTt2MXl-kBDR9vgPB1z6K7naR_2kZo-9kPMIVMbYmjN9by8C8MNNdSZ3E-iyYc0-y7H9Jg8qE2T_ZPje0q-bi-_XLwtrj69eXfx6qpwAkAUlnFwDoUXyKVSUkJpERGm5K7EGozdCOWErzlfc6xKW6JXrlLSygqsVHhKzhZvn-Lt6POg25DnbKbzccwaFFNyjQo3_0fXopQoGc7WFwvqUsw5-Vr3aYqcDhqYnmvT9U4D6qW2CX92NI-29dVf-E9PE6AW4C40_vBPmd5-fs237wGP7t-CgKYp</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1549636038</pqid></control><display><type>article</type><title>Evaluation of therapeutic effects of natural killer (NK) cell‐based immunotherapy in mice using in vivo apoptosis bioimaging with a caspase‐3 sensor</title><source>MEDLINE</source><source>Wiley Online Library All Journals</source><source>Alma/SFX Local Collection</source><creator>Lee, Ho Won ; Singh, Thoudam Debraj ; Lee, Sang‐Woo ; Ha, Jeoung‐Hee ; Rehemtulla, Alnawaz ; Ahn, Byeong‐Cheol ; Jeon, Young Hyun ; Lee, Jaetae</creator><creatorcontrib>Lee, Ho Won ; Singh, Thoudam Debraj ; Lee, Sang‐Woo ; Ha, Jeoung‐Hee ; Rehemtulla, Alnawaz ; Ahn, Byeong‐Cheol ; Jeon, Young Hyun ; Lee, Jaetae</creatorcontrib><description>ABSTRACT
Natural killer (NK) cell‐based immunotherapy is a promising strategy for cancer treatment, and caspase‐3 is an important effector molecule in NK cell‐mediated apoptosis in cancers. Here, we evaluated the antitumor effects of NK cell‐based immunotherapy by serial noninvasive imaging of apoptosis using a caspase‐3 sensor in mice with human glioma xenografts. Human glioma cells expressing both a caspase‐3 sensor as a surrogate marker for caspase‐3 activation and Renilla luciferase (Rluc) as a surrogate marker for cell viability were established and referred to as D54‐CR cells. Human NK92 cells were used as effector cells. Treatment with NK92 cells resulted in a time‐ and effector number‐dependent increase in bioluminescence imaging (BLI) activity of the caspase‐3 sensor in D54‐CR cells in vitro. Caspase‐3 activation by NK92 treatment was blocked by Z‐VAD treatment in D54‐CR cells. Transfusion of NK92 cells induced an increase of the BLI signal by caspase‐3 activation in a dose‐ and time‐dependent manner in D54‐CR tumor‐bearing mice but not in PBS‐treated mice. Accordingly, sequential BLI with the Rluc reporter gene revealed marked retardation of tumor growth in the NK92‐treatment group but not in the PBS‐treatment group. These data suggest that noninvasive imaging of apoptosis with a caspase‐3 sensor can be used as an effective tool for evaluation of therapeutic efficacy as well as for optimization of NK cell‐based immunotherapy.—Lee, H. W., Singh, T. D., Lee, S.‐W., Ha, J.‐H., Rehemtulla, A., Ahn, B.‐C., Jeon, Y.‐H., Lee, J. Evaluation of therapeutic effects of natural killer (NK) cell‐based immunotherapy in mice using in vivo apoptosis bioimaging with a caspase‐3 sensor. FASEB J. 28, 2932–2941 (2014). www.fasebj.org</description><identifier>ISSN: 0892-6638</identifier><identifier>EISSN: 1530-6860</identifier><identifier>DOI: 10.1096/fj.13-243014</identifier><identifier>PMID: 24736413</identifier><language>eng</language><publisher>United States: Federation of American Societies for Experimental Biology</publisher><subject>Animals ; Antineoplastic Agents - immunology ; Apoptosis - immunology ; Caspase 3 - immunology ; Cell Line ; Cell Line, Tumor ; Cell Survival - immunology ; glioma ; Glioma - immunology ; Glioma - therapy ; Humans ; Immunotherapy - methods ; Killer Cells, Natural - immunology ; Mice ; molecular imaging ; Renilla ; Renilla luciferase</subject><ispartof>The FASEB journal, 2014-07, Vol.28 (7), p.2932-2941</ispartof><rights>FASEB</rights><rights>FASEB.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4114-b021cc34e4326886619b3331892c93f1ab748c4ef22523d9b93e8cd86b6d1b683</citedby><cites>FETCH-LOGICAL-c4114-b021cc34e4326886619b3331892c93f1ab748c4ef22523d9b93e8cd86b6d1b683</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1096%2Ffj.13-243014$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1096%2Ffj.13-243014$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24736413$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, Ho Won</creatorcontrib><creatorcontrib>Singh, Thoudam Debraj</creatorcontrib><creatorcontrib>Lee, Sang‐Woo</creatorcontrib><creatorcontrib>Ha, Jeoung‐Hee</creatorcontrib><creatorcontrib>Rehemtulla, Alnawaz</creatorcontrib><creatorcontrib>Ahn, Byeong‐Cheol</creatorcontrib><creatorcontrib>Jeon, Young Hyun</creatorcontrib><creatorcontrib>Lee, Jaetae</creatorcontrib><title>Evaluation of therapeutic effects of natural killer (NK) cell‐based immunotherapy in mice using in vivo apoptosis bioimaging with a caspase‐3 sensor</title><title>The FASEB journal</title><addtitle>FASEB J</addtitle><description>ABSTRACT
Natural killer (NK) cell‐based immunotherapy is a promising strategy for cancer treatment, and caspase‐3 is an important effector molecule in NK cell‐mediated apoptosis in cancers. Here, we evaluated the antitumor effects of NK cell‐based immunotherapy by serial noninvasive imaging of apoptosis using a caspase‐3 sensor in mice with human glioma xenografts. Human glioma cells expressing both a caspase‐3 sensor as a surrogate marker for caspase‐3 activation and Renilla luciferase (Rluc) as a surrogate marker for cell viability were established and referred to as D54‐CR cells. Human NK92 cells were used as effector cells. Treatment with NK92 cells resulted in a time‐ and effector number‐dependent increase in bioluminescence imaging (BLI) activity of the caspase‐3 sensor in D54‐CR cells in vitro. Caspase‐3 activation by NK92 treatment was blocked by Z‐VAD treatment in D54‐CR cells. Transfusion of NK92 cells induced an increase of the BLI signal by caspase‐3 activation in a dose‐ and time‐dependent manner in D54‐CR tumor‐bearing mice but not in PBS‐treated mice. Accordingly, sequential BLI with the Rluc reporter gene revealed marked retardation of tumor growth in the NK92‐treatment group but not in the PBS‐treatment group. These data suggest that noninvasive imaging of apoptosis with a caspase‐3 sensor can be used as an effective tool for evaluation of therapeutic efficacy as well as for optimization of NK cell‐based immunotherapy.—Lee, H. W., Singh, T. D., Lee, S.‐W., Ha, J.‐H., Rehemtulla, A., Ahn, B.‐C., Jeon, Y.‐H., Lee, J. Evaluation of therapeutic effects of natural killer (NK) cell‐based immunotherapy in mice using in vivo apoptosis bioimaging with a caspase‐3 sensor. FASEB J. 28, 2932–2941 (2014). www.fasebj.org</description><subject>Animals</subject><subject>Antineoplastic Agents - immunology</subject><subject>Apoptosis - immunology</subject><subject>Caspase 3 - immunology</subject><subject>Cell Line</subject><subject>Cell Line, Tumor</subject><subject>Cell Survival - immunology</subject><subject>glioma</subject><subject>Glioma - immunology</subject><subject>Glioma - therapy</subject><subject>Humans</subject><subject>Immunotherapy - methods</subject><subject>Killer Cells, Natural - immunology</subject><subject>Mice</subject><subject>molecular imaging</subject><subject>Renilla</subject><subject>Renilla luciferase</subject><issn>0892-6638</issn><issn>1530-6860</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAURi0EokNhxxp5WSRSfH09HmcJVYe_ChbA2rIdp_WQxKmdTDU7HoElz8eTkCgDS1hZ1_fcI336CHkK7BxYKV_Wu3PAggtkIO6RFayRFVJJdp-smCp5ISWqE_Io5x1jDBjIh-SEiw1KAbgiPy_3phnNEGJHY02HG59M78chOOrr2rshz9-dGcZkGvotNI1P9Ozjh-fU-ab59f2HNdlXNLTt2MXl-kBDR9vgPB1z6K7naR_2kZo-9kPMIVMbYmjN9by8C8MNNdSZ3E-iyYc0-y7H9Jg8qE2T_ZPje0q-bi-_XLwtrj69eXfx6qpwAkAUlnFwDoUXyKVSUkJpERGm5K7EGozdCOWErzlfc6xKW6JXrlLSygqsVHhKzhZvn-Lt6POg25DnbKbzccwaFFNyjQo3_0fXopQoGc7WFwvqUsw5-Vr3aYqcDhqYnmvT9U4D6qW2CX92NI-29dVf-E9PE6AW4C40_vBPmd5-fs237wGP7t-CgKYp</recordid><startdate>201407</startdate><enddate>201407</enddate><creator>Lee, Ho Won</creator><creator>Singh, Thoudam Debraj</creator><creator>Lee, Sang‐Woo</creator><creator>Ha, Jeoung‐Hee</creator><creator>Rehemtulla, Alnawaz</creator><creator>Ahn, Byeong‐Cheol</creator><creator>Jeon, Young Hyun</creator><creator>Lee, Jaetae</creator><general>Federation of American Societies for Experimental Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>201407</creationdate><title>Evaluation of therapeutic effects of natural killer (NK) cell‐based immunotherapy in mice using in vivo apoptosis bioimaging with a caspase‐3 sensor</title><author>Lee, Ho Won ; Singh, Thoudam Debraj ; Lee, Sang‐Woo ; Ha, Jeoung‐Hee ; Rehemtulla, Alnawaz ; Ahn, Byeong‐Cheol ; Jeon, Young Hyun ; Lee, Jaetae</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4114-b021cc34e4326886619b3331892c93f1ab748c4ef22523d9b93e8cd86b6d1b683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Antineoplastic Agents - immunology</topic><topic>Apoptosis - immunology</topic><topic>Caspase 3 - immunology</topic><topic>Cell Line</topic><topic>Cell Line, Tumor</topic><topic>Cell Survival - immunology</topic><topic>glioma</topic><topic>Glioma - immunology</topic><topic>Glioma - therapy</topic><topic>Humans</topic><topic>Immunotherapy - methods</topic><topic>Killer Cells, Natural - immunology</topic><topic>Mice</topic><topic>molecular imaging</topic><topic>Renilla</topic><topic>Renilla luciferase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Ho Won</creatorcontrib><creatorcontrib>Singh, Thoudam Debraj</creatorcontrib><creatorcontrib>Lee, Sang‐Woo</creatorcontrib><creatorcontrib>Ha, Jeoung‐Hee</creatorcontrib><creatorcontrib>Rehemtulla, Alnawaz</creatorcontrib><creatorcontrib>Ahn, Byeong‐Cheol</creatorcontrib><creatorcontrib>Jeon, Young Hyun</creatorcontrib><creatorcontrib>Lee, Jaetae</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>The FASEB journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Ho Won</au><au>Singh, Thoudam Debraj</au><au>Lee, Sang‐Woo</au><au>Ha, Jeoung‐Hee</au><au>Rehemtulla, Alnawaz</au><au>Ahn, Byeong‐Cheol</au><au>Jeon, Young Hyun</au><au>Lee, Jaetae</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of therapeutic effects of natural killer (NK) cell‐based immunotherapy in mice using in vivo apoptosis bioimaging with a caspase‐3 sensor</atitle><jtitle>The FASEB journal</jtitle><addtitle>FASEB J</addtitle><date>2014-07</date><risdate>2014</risdate><volume>28</volume><issue>7</issue><spage>2932</spage><epage>2941</epage><pages>2932-2941</pages><issn>0892-6638</issn><eissn>1530-6860</eissn><abstract>ABSTRACT
Natural killer (NK) cell‐based immunotherapy is a promising strategy for cancer treatment, and caspase‐3 is an important effector molecule in NK cell‐mediated apoptosis in cancers. Here, we evaluated the antitumor effects of NK cell‐based immunotherapy by serial noninvasive imaging of apoptosis using a caspase‐3 sensor in mice with human glioma xenografts. Human glioma cells expressing both a caspase‐3 sensor as a surrogate marker for caspase‐3 activation and Renilla luciferase (Rluc) as a surrogate marker for cell viability were established and referred to as D54‐CR cells. Human NK92 cells were used as effector cells. Treatment with NK92 cells resulted in a time‐ and effector number‐dependent increase in bioluminescence imaging (BLI) activity of the caspase‐3 sensor in D54‐CR cells in vitro. Caspase‐3 activation by NK92 treatment was blocked by Z‐VAD treatment in D54‐CR cells. Transfusion of NK92 cells induced an increase of the BLI signal by caspase‐3 activation in a dose‐ and time‐dependent manner in D54‐CR tumor‐bearing mice but not in PBS‐treated mice. Accordingly, sequential BLI with the Rluc reporter gene revealed marked retardation of tumor growth in the NK92‐treatment group but not in the PBS‐treatment group. These data suggest that noninvasive imaging of apoptosis with a caspase‐3 sensor can be used as an effective tool for evaluation of therapeutic efficacy as well as for optimization of NK cell‐based immunotherapy.—Lee, H. W., Singh, T. D., Lee, S.‐W., Ha, J.‐H., Rehemtulla, A., Ahn, B.‐C., Jeon, Y.‐H., Lee, J. Evaluation of therapeutic effects of natural killer (NK) cell‐based immunotherapy in mice using in vivo apoptosis bioimaging with a caspase‐3 sensor. FASEB J. 28, 2932–2941 (2014). www.fasebj.org</abstract><cop>United States</cop><pub>Federation of American Societies for Experimental Biology</pub><pmid>24736413</pmid><doi>10.1096/fj.13-243014</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0892-6638 |
| ispartof | The FASEB journal, 2014-07, Vol.28 (7), p.2932-2941 |
| issn | 0892-6638 1530-6860 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1808653837 |
| source | MEDLINE; Wiley Online Library All Journals; Alma/SFX Local Collection |
| subjects | Animals Antineoplastic Agents - immunology Apoptosis - immunology Caspase 3 - immunology Cell Line Cell Line, Tumor Cell Survival - immunology glioma Glioma - immunology Glioma - therapy Humans Immunotherapy - methods Killer Cells, Natural - immunology Mice molecular imaging Renilla Renilla luciferase |
| title | Evaluation of therapeutic effects of natural killer (NK) cell‐based immunotherapy in mice using in vivo apoptosis bioimaging with a caspase‐3 sensor |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-11T23%3A34%3A24IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Evaluation%20of%20therapeutic%20effects%20of%20natural%20killer%20(NK)%20cell%E2%80%90based%20immunotherapy%20in%20mice%20using%20in%20vivo%20apoptosis%20bioimaging%20with%20a%20caspase%E2%80%903%20sensor&rft.jtitle=The%20FASEB%20journal&rft.au=Lee,%20Ho%20Won&rft.date=2014-07&rft.volume=28&rft.issue=7&rft.spage=2932&rft.epage=2941&rft.pages=2932-2941&rft.issn=0892-6638&rft.eissn=1530-6860&rft_id=info:doi/10.1096/fj.13-243014&rft_dat=%3Cproquest_cross%3E1549636038%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1549636038&rft_id=info:pmid/24736413&rfr_iscdi=true |