Detection of variant infectious bronchitis viruses in Sri Lanka (2012-2015)
Poultry production is an important sector of agriculture in Sri Lanka; however, there is a lack of information regarding circulation of infectious bronchitis virus (IBV). RNA was extracted from chicken tissues, subjected to IBV S1 RT-PCR, and sequenced. Overall, 19 out of 34 (55.88 %) samples were I...
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| Veröffentlicht in: | Archives of virology 2016-06, Vol.161 (6), p.1697-1699 |
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| creator | Ball, Christopher Forrester, Anne Ganapathy, Kannan |
| description | Poultry production is an important sector of agriculture in Sri Lanka; however, there is a lack of information regarding circulation of infectious bronchitis virus (IBV). RNA was extracted from chicken tissues, subjected to IBV S1 RT-PCR, and sequenced. Overall, 19 out of 34 (55.88 %) samples were IBV positive and contained the genotype 793B (n = 13; 68.42 %), D274 (n = 4; 21.05 %) or Massachusetts (n = 2; 10.53 %). All three genotypes contained at least one strain with less than 99 % nucleotide sequence identity to the corresponding vaccine strains. This report identified co-circulation of IBV strains 793B, Massachusetts and D274, in Sri Lanka that are divergent from the respective vaccine strains. |
| doi_str_mv | 10.1007/s00705-016-2831-x |
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RNA was extracted from chicken tissues, subjected to IBV S1 RT-PCR, and sequenced. Overall, 19 out of 34 (55.88 %) samples were IBV positive and contained the genotype 793B (n = 13; 68.42 %), D274 (n = 4; 21.05 %) or Massachusetts (n = 2; 10.53 %). All three genotypes contained at least one strain with less than 99 % nucleotide sequence identity to the corresponding vaccine strains. 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RNA was extracted from chicken tissues, subjected to IBV S1 RT-PCR, and sequenced. Overall, 19 out of 34 (55.88 %) samples were IBV positive and contained the genotype 793B (n = 13; 68.42 %), D274 (n = 4; 21.05 %) or Massachusetts (n = 2; 10.53 %). All three genotypes contained at least one strain with less than 99 % nucleotide sequence identity to the corresponding vaccine strains. This report identified co-circulation of IBV strains 793B, Massachusetts and D274, in Sri Lanka that are divergent from the respective vaccine strains.</description><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Brief Report</subject><subject>Bronchitis</subject><subject>Chickens - virology</subject><subject>Coronavirus Infections - veterinary</subject><subject>Coronavirus Infections - virology</subject><subject>Farms</subject><subject>Genetic Variation</subject><subject>Genotype</subject><subject>Genotype & phenotype</subject><subject>Infectious bronchitis virus</subject><subject>Infectious bronchitis virus - genetics</subject><subject>Infectious bronchitis virus - immunology</subject><subject>Infectious bronchitis virus - isolation & purification</subject><subject>Infectious Diseases</subject><subject>Medical Microbiology</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Poultry</subject><subject>Poultry Diseases - virology</subject><subject>RNA, Viral - genetics</subject><subject>Sri Lanka</subject><subject>Vaccines</subject><subject>Viral Vaccines - genetics</subject><subject>Virology</subject><subject>Virulence</subject><subject>Viruses</subject><issn>0304-8608</issn><issn>1432-8798</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkcFOGzEQhi3UqgmhD8AFrdQLHNyO7fXaPla00IpIHApny3HGrWnipfZuBG-PIWmFkCokayzNfPPP2D8hhww-MgD1qdQAkgLrKNeC0bs9MmWt4FQro9-QKQhoqe5AT8h-KTcANSHkOzLhCjhIBVNy8QUH9EPsU9OHZuNydGloYgpPybE0i9wn_ysOsTSbmMeCpVabHzk2c5d-u-aYA-O0BnlyQN4Gtyr4fnfPyPXZ16vTb3R-ef799POc-hbkQAMqvXAdY8tg0AiFHSxRKgTvHNYFg1OeC80NCCekqceL4F3AVqHUvBMzcrzVvc39nxHLYNexeFytXMK6smUadNcKVb_iVVQZaDtmWqjohxfoTT_mVB9SKW0UtFKbSrEt5XNfSsZgb3Ncu3xvGdhHU-zWFFtNsY-m2Lvac7RTHhdrXP7r-OtCBfgWKLWUfmJ-Nvq_qg8AkZRr</recordid><startdate>20160601</startdate><enddate>20160601</enddate><creator>Ball, Christopher</creator><creator>Forrester, Anne</creator><creator>Ganapathy, Kannan</creator><general>Springer Vienna</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20160601</creationdate><title>Detection of variant infectious bronchitis viruses in Sri Lanka (2012-2015)</title><author>Ball, Christopher ; 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RNA was extracted from chicken tissues, subjected to IBV S1 RT-PCR, and sequenced. Overall, 19 out of 34 (55.88 %) samples were IBV positive and contained the genotype 793B (n = 13; 68.42 %), D274 (n = 4; 21.05 %) or Massachusetts (n = 2; 10.53 %). All three genotypes contained at least one strain with less than 99 % nucleotide sequence identity to the corresponding vaccine strains. This report identified co-circulation of IBV strains 793B, Massachusetts and D274, in Sri Lanka that are divergent from the respective vaccine strains.</abstract><cop>Vienna</cop><pub>Springer Vienna</pub><pmid>27020570</pmid><doi>10.1007/s00705-016-2831-x</doi><tpages>3</tpages></addata></record> |
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| subjects | Animals Biomedical and Life Sciences Biomedicine Brief Report Bronchitis Chickens - virology Coronavirus Infections - veterinary Coronavirus Infections - virology Farms Genetic Variation Genotype Genotype & phenotype Infectious bronchitis virus Infectious bronchitis virus - genetics Infectious bronchitis virus - immunology Infectious bronchitis virus - isolation & purification Infectious Diseases Medical Microbiology Phylogenetics Phylogeny Poultry Poultry Diseases - virology RNA, Viral - genetics Sri Lanka Vaccines Viral Vaccines - genetics Virology Virulence Viruses |
| title | Detection of variant infectious bronchitis viruses in Sri Lanka (2012-2015) |
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