P51 PREPARATION OF ASIATIC ACID LOADED NANOPARTICLES FOR DELIVERY ACROSS THE BLOOD BRAIN BARRIER AND ITS EFFECTS ON GLIOBLASTOMA CELLS IN VITRO

P51 PREPARATION OF ASIATIC ACID LOADED NANOPARTICLES FOR DELIVERY ACROSS THE BLOOD BRAIN BARRIER AND ITS EFFECTS ON GLIOBLASTOMA CELLS IN VITRO

INTRODUCTION: Drug delivery across tight junctions of the blood brain barrier poses a significant challenge to glioma treatment. Biodegradable nanoparticles (NPs) formulated with poly- epsilon -caprolactone were prepared to investigate sustained delivery of asiatic acid (AA). METHOD: Blank & dru...

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Veröffentlicht in:Neuro-oncology (Charlottesville, Va.) Va.), 2014-10, Vol.16 (suppl 6), p.vi8-vi8
Hauptverfasser: Thakor, Flourina, Welsby, Philip J, Wan, Ka-Wai, Welsby, Gail
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container_end_page vi8
container_issue suppl 6
container_start_page vi8
container_title Neuro-oncology (Charlottesville, Va.)
container_volume 16
creator Thakor, Flourina
Welsby, Philip J
Wan, Ka-Wai
Welsby, Gail
description INTRODUCTION: Drug delivery across tight junctions of the blood brain barrier poses a significant challenge to glioma treatment. Biodegradable nanoparticles (NPs) formulated with poly- epsilon -caprolactone were prepared to investigate sustained delivery of asiatic acid (AA). METHOD: Blank & drug-loaded NPs were prepared by the solvent displacement method using a two-phase system consisting of acetone & water; drug (AA or cisplatin) was dissolved in the organic phase. NPs were characterised for stability, encapsulation efficiency, biodegradation & rate of drug release. The IC50 values for AA & cisplatin in SVGP12 and U87-MG cell lines were also established. RESULTS: Blank NPs were produced in the range of 131-148nm ( Day 0: 133 plus or minus 2; Day 60: 142 plus or minus 5), with AA- loaded particles in the range of 150-151nm ( Day 0: 153 plus or minus 3; Day 60: 150 plus or minus 1). NPs had a negative surface charge of -17 plus or minus 1 mV to -31 plus or minus 3 mV and -20 plus or minus 1 mV to -30 plus or minus 1 mV for blank & AA-loaded NPs, respectively. Polydispersity index was
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Biodegradable nanoparticles (NPs) formulated with poly- epsilon -caprolactone were prepared to investigate sustained delivery of asiatic acid (AA). METHOD: Blank & drug-loaded NPs were prepared by the solvent displacement method using a two-phase system consisting of acetone & water; drug (AA or cisplatin) was dissolved in the organic phase. NPs were characterised for stability, encapsulation efficiency, biodegradation & rate of drug release. The IC50 values for AA & cisplatin in SVGP12 and U87-MG cell lines were also established. RESULTS: Blank NPs were produced in the range of 131-148nm ( Day 0: 133 plus or minus 2; Day 60: 142 plus or minus 5), with AA- loaded particles in the range of 150-151nm ( Day 0: 153 plus or minus 3; Day 60: 150 plus or minus 1). NPs had a negative surface charge of -17 plus or minus 1 mV to -31 plus or minus 3 mV and -20 plus or minus 1 mV to -30 plus or minus 1 mV for blank & AA-loaded NPs, respectively. Polydispersity index was <1 demonstrating a monodisperse, uniform polymer was created. NPs were stable for a minimum of 60 days. AA-loaded NPs had a drug loading efficiency of 28 plus or minus 1.2%, showing a sustained drug release in phosphate buffered saline at pH 7.4 & pH 5.5 over 24hrs. An enhanced drug release of 65 plus or minus 4% at the end of 24hrs along with NP degradation was observed in the presence of lipase. The IC50 values for AA & cisplatin at 48hrs were 67mM & 10mM for SVGP12 and 50mM & 7mM for U87-MG cells respectively. CONCLUSION: This study provides a method for the preparation of stable, monodispersed NPs containing AA, showing a sustained drug release. Future work will examine the ability of the NPs to cross the blood brain barrier using an in vitro model system.]]></description><identifier>ISSN: 1522-8517</identifier><identifier>DOI: 10.1093/neuonc/nou249.39</identifier><language>eng</language><ispartof>Neuro-oncology (Charlottesville, Va.), 2014-10, Vol.16 (suppl 6), p.vi8-vi8</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Thakor, Flourina</creatorcontrib><creatorcontrib>Welsby, Philip J</creatorcontrib><creatorcontrib>Wan, Ka-Wai</creatorcontrib><creatorcontrib>Welsby, Gail</creatorcontrib><title>P51 PREPARATION OF ASIATIC ACID LOADED NANOPARTICLES FOR DELIVERY ACROSS THE BLOOD BRAIN BARRIER AND ITS EFFECTS ON GLIOBLASTOMA CELLS IN VITRO</title><title>Neuro-oncology (Charlottesville, Va.)</title><description><![CDATA[INTRODUCTION: Drug delivery across tight junctions of the blood brain barrier poses a significant challenge to glioma treatment. Biodegradable nanoparticles (NPs) formulated with poly- epsilon -caprolactone were prepared to investigate sustained delivery of asiatic acid (AA). METHOD: Blank & drug-loaded NPs were prepared by the solvent displacement method using a two-phase system consisting of acetone & water; drug (AA or cisplatin) was dissolved in the organic phase. NPs were characterised for stability, encapsulation efficiency, biodegradation & rate of drug release. The IC50 values for AA & cisplatin in SVGP12 and U87-MG cell lines were also established. RESULTS: Blank NPs were produced in the range of 131-148nm ( Day 0: 133 plus or minus 2; Day 60: 142 plus or minus 5), with AA- loaded particles in the range of 150-151nm ( Day 0: 153 plus or minus 3; Day 60: 150 plus or minus 1). NPs had a negative surface charge of -17 plus or minus 1 mV to -31 plus or minus 3 mV and -20 plus or minus 1 mV to -30 plus or minus 1 mV for blank & AA-loaded NPs, respectively. Polydispersity index was <1 demonstrating a monodisperse, uniform polymer was created. NPs were stable for a minimum of 60 days. AA-loaded NPs had a drug loading efficiency of 28 plus or minus 1.2%, showing a sustained drug release in phosphate buffered saline at pH 7.4 & pH 5.5 over 24hrs. An enhanced drug release of 65 plus or minus 4% at the end of 24hrs along with NP degradation was observed in the presence of lipase. The IC50 values for AA & cisplatin at 48hrs were 67mM & 10mM for SVGP12 and 50mM & 7mM for U87-MG cells respectively. CONCLUSION: This study provides a method for the preparation of stable, monodispersed NPs containing AA, showing a sustained drug release. 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Biodegradable nanoparticles (NPs) formulated with poly- epsilon -caprolactone were prepared to investigate sustained delivery of asiatic acid (AA). METHOD: Blank & drug-loaded NPs were prepared by the solvent displacement method using a two-phase system consisting of acetone & water; drug (AA or cisplatin) was dissolved in the organic phase. NPs were characterised for stability, encapsulation efficiency, biodegradation & rate of drug release. The IC50 values for AA & cisplatin in SVGP12 and U87-MG cell lines were also established. RESULTS: Blank NPs were produced in the range of 131-148nm ( Day 0: 133 plus or minus 2; Day 60: 142 plus or minus 5), with AA- loaded particles in the range of 150-151nm ( Day 0: 153 plus or minus 3; Day 60: 150 plus or minus 1). NPs had a negative surface charge of -17 plus or minus 1 mV to -31 plus or minus 3 mV and -20 plus or minus 1 mV to -30 plus or minus 1 mV for blank & AA-loaded NPs, respectively. Polydispersity index was <1 demonstrating a monodisperse, uniform polymer was created. NPs were stable for a minimum of 60 days. AA-loaded NPs had a drug loading efficiency of 28 plus or minus 1.2%, showing a sustained drug release in phosphate buffered saline at pH 7.4 & pH 5.5 over 24hrs. An enhanced drug release of 65 plus or minus 4% at the end of 24hrs along with NP degradation was observed in the presence of lipase. The IC50 values for AA & cisplatin at 48hrs were 67mM & 10mM for SVGP12 and 50mM & 7mM for U87-MG cells respectively. CONCLUSION: This study provides a method for the preparation of stable, monodispersed NPs containing AA, showing a sustained drug release. Future work will examine the ability of the NPs to cross the blood brain barrier using an in vitro model system.]]></abstract><doi>10.1093/neuonc/nou249.39</doi></addata></record>
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title P51 PREPARATION OF ASIATIC ACID LOADED NANOPARTICLES FOR DELIVERY ACROSS THE BLOOD BRAIN BARRIER AND ITS EFFECTS ON GLIOBLASTOMA CELLS IN VITRO
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