Genome-wide association study for sperm membrane integrity in frozen-thawed semen of Holstein-Friesian bulls
•First genome-wise association study for sperm membrane integrity in Holstein bulls.•SNP on chromosome 6 significantly associated with sperm plasma membrane integrity.•Three candidate genes involved in sperm plasma membrane integrity in thawed semen. The aim of the study was to screen the entire bul...
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| Veröffentlicht in: | Animal reproduction science 2016-07, Vol.170, p.135-140 |
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| creator | Kamiński, Stanisław Hering, Dorota M. Oleński, Kamil Lecewicz, Marek Kordan, Władysław |
| description | •First genome-wise association study for sperm membrane integrity in Holstein bulls.•SNP on chromosome 6 significantly associated with sperm plasma membrane integrity.•Three candidate genes involved in sperm plasma membrane integrity in thawed semen.
The aim of the study was to screen the entire bull genome to identify SNP markers and propose candidate genes potentially involved in the variation of sperm membrane integrity in Holstein-Friesian bulls. Two hundred eighty eight bulls kept in one AI center were included in the study. Each bull was genotyped for 54.001 Single Nucleotide Polymorpisms (SNP) by the Illumina BovineSNP50 BeadChip. Commercial straws of frozen-thawed semen were used for the evaluation of sperm plasma membrane integrity (SYBR-14/PI staining) and sperm mitochondrial function (JC1/PI staining). An additive model for Linear Regression Analysis was applied to estimate the effect of SNP marker for sperm membrane integrity (by the use of GoldenHelix SVS7 software). Five significant markers (encompassing 2,2 MB region located on chromosome 6) for SYBR-14/PI were found. Among them one marker—rs41570391 passed Bonferroni correction test. Within approximately 3 Mb genomic region including significant markers three candidate genes: SGMS2 (Sphingomyelin Synthase 2), TET2 (Methylcytosine dioxygenase 2) and GSTCD genes (Gluthatione S-transferase C terminal domain) were proposed as potentially involved in sperm membrane integrity in frozen-thawed semen of Holstein-Friesian bulls. |
| doi_str_mv | 10.1016/j.anireprosci.2016.05.002 |
| format | Article |
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The aim of the study was to screen the entire bull genome to identify SNP markers and propose candidate genes potentially involved in the variation of sperm membrane integrity in Holstein-Friesian bulls. Two hundred eighty eight bulls kept in one AI center were included in the study. Each bull was genotyped for 54.001 Single Nucleotide Polymorpisms (SNP) by the Illumina BovineSNP50 BeadChip. Commercial straws of frozen-thawed semen were used for the evaluation of sperm plasma membrane integrity (SYBR-14/PI staining) and sperm mitochondrial function (JC1/PI staining). An additive model for Linear Regression Analysis was applied to estimate the effect of SNP marker for sperm membrane integrity (by the use of GoldenHelix SVS7 software). Five significant markers (encompassing 2,2 MB region located on chromosome 6) for SYBR-14/PI were found. Among them one marker—rs41570391 passed Bonferroni correction test. Within approximately 3 Mb genomic region including significant markers three candidate genes: SGMS2 (Sphingomyelin Synthase 2), TET2 (Methylcytosine dioxygenase 2) and GSTCD genes (Gluthatione S-transferase C terminal domain) were proposed as potentially involved in sperm membrane integrity in frozen-thawed semen of Holstein-Friesian bulls.</description><identifier>ISSN: 0378-4320</identifier><identifier>EISSN: 1873-2232</identifier><identifier>DOI: 10.1016/j.anireprosci.2016.05.002</identifier><identifier>PMID: 27236378</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Bull ; Cattle ; Cell Membrane - physiology ; Cryopreservation - veterinary ; Freezing ; Gene Expression Regulation ; Genome-wide association study ; Genome-Wide Association Study - veterinary ; Genotype ; Male ; Polymorphism, Single Nucleotide ; Semen Preservation - veterinary ; SNP ; Sperm membrane</subject><ispartof>Animal reproduction science, 2016-07, Vol.170, p.135-140</ispartof><rights>2016 Elsevier B.V.</rights><rights>Copyright © 2016 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c410t-14306f99ac6d44759373663dcd0d5c1bd6d6726e168346bafb37c567f34db62f3</citedby><cites>FETCH-LOGICAL-c410t-14306f99ac6d44759373663dcd0d5c1bd6d6726e168346bafb37c567f34db62f3</cites><orcidid>0000-0002-5538-1352</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.anireprosci.2016.05.002$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27236378$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kamiński, Stanisław</creatorcontrib><creatorcontrib>Hering, Dorota M.</creatorcontrib><creatorcontrib>Oleński, Kamil</creatorcontrib><creatorcontrib>Lecewicz, Marek</creatorcontrib><creatorcontrib>Kordan, Władysław</creatorcontrib><title>Genome-wide association study for sperm membrane integrity in frozen-thawed semen of Holstein-Friesian bulls</title><title>Animal reproduction science</title><addtitle>Anim Reprod Sci</addtitle><description>•First genome-wise association study for sperm membrane integrity in Holstein bulls.•SNP on chromosome 6 significantly associated with sperm plasma membrane integrity.•Three candidate genes involved in sperm plasma membrane integrity in thawed semen.
The aim of the study was to screen the entire bull genome to identify SNP markers and propose candidate genes potentially involved in the variation of sperm membrane integrity in Holstein-Friesian bulls. Two hundred eighty eight bulls kept in one AI center were included in the study. Each bull was genotyped for 54.001 Single Nucleotide Polymorpisms (SNP) by the Illumina BovineSNP50 BeadChip. Commercial straws of frozen-thawed semen were used for the evaluation of sperm plasma membrane integrity (SYBR-14/PI staining) and sperm mitochondrial function (JC1/PI staining). An additive model for Linear Regression Analysis was applied to estimate the effect of SNP marker for sperm membrane integrity (by the use of GoldenHelix SVS7 software). Five significant markers (encompassing 2,2 MB region located on chromosome 6) for SYBR-14/PI were found. Among them one marker—rs41570391 passed Bonferroni correction test. Within approximately 3 Mb genomic region including significant markers three candidate genes: SGMS2 (Sphingomyelin Synthase 2), TET2 (Methylcytosine dioxygenase 2) and GSTCD genes (Gluthatione S-transferase C terminal domain) were proposed as potentially involved in sperm membrane integrity in frozen-thawed semen of Holstein-Friesian bulls.</description><subject>Animals</subject><subject>Bull</subject><subject>Cattle</subject><subject>Cell Membrane - physiology</subject><subject>Cryopreservation - veterinary</subject><subject>Freezing</subject><subject>Gene Expression Regulation</subject><subject>Genome-wide association study</subject><subject>Genome-Wide Association Study - veterinary</subject><subject>Genotype</subject><subject>Male</subject><subject>Polymorphism, Single Nucleotide</subject><subject>Semen Preservation - veterinary</subject><subject>SNP</subject><subject>Sperm membrane</subject><issn>0378-4320</issn><issn>1873-2232</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU9v3CAQxVHUKNkk_QoVvfVihz822Mdq1SSVIuWSnBGGoWVlwxZwo-2nL9GmVW_piRHz5s3o_RD6SElLCRXXu1YHn2CfYja-ZfWrJX1LCDtBGzpI3jDG2Tu0IVwOTccZOUcXOe8IIVKI8QydM8m4qM0Nmm8hxAWaZ28B65yj8br4GHAuqz1gFxPOe0gLXmCZkg6AfSjwLflyqBV2Kf6C0JTv-hkszrBAwNHhuzjnAj40N8lD9jrgaZ3nfIVOnZ4zvH99L9HTzZfH7V1z_3D7dfv5vjEdJaWhHSfCjaM2wnad7EcuuRDcGktsb-hkhRWSCaBi4J2YtJu4NL2Qjnd2EszxS_Tp6FsT-rFCLmrx2cA81_vjmhUdyCB4XwN4WyrHfhCSMlml41Fqau45gVP75BedDooS9cJF7dQ_XNQLF0V6VbnU2Q-va9ZpAft38g-IKtgeBVBz-ekhqWoBwYCthqYoG_1_rPkNhtmmHQ</recordid><startdate>201607</startdate><enddate>201607</enddate><creator>Kamiński, Stanisław</creator><creator>Hering, Dorota M.</creator><creator>Oleński, Kamil</creator><creator>Lecewicz, Marek</creator><creator>Kordan, Władysław</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><orcidid>https://orcid.org/0000-0002-5538-1352</orcidid></search><sort><creationdate>201607</creationdate><title>Genome-wide association study for sperm membrane integrity in frozen-thawed semen of Holstein-Friesian bulls</title><author>Kamiński, Stanisław ; Hering, Dorota M. ; Oleński, Kamil ; Lecewicz, Marek ; Kordan, Władysław</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c410t-14306f99ac6d44759373663dcd0d5c1bd6d6726e168346bafb37c567f34db62f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Bull</topic><topic>Cattle</topic><topic>Cell Membrane - physiology</topic><topic>Cryopreservation - veterinary</topic><topic>Freezing</topic><topic>Gene Expression Regulation</topic><topic>Genome-wide association study</topic><topic>Genome-Wide Association Study - veterinary</topic><topic>Genotype</topic><topic>Male</topic><topic>Polymorphism, Single Nucleotide</topic><topic>Semen Preservation - veterinary</topic><topic>SNP</topic><topic>Sperm membrane</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kamiński, Stanisław</creatorcontrib><creatorcontrib>Hering, Dorota M.</creatorcontrib><creatorcontrib>Oleński, Kamil</creatorcontrib><creatorcontrib>Lecewicz, Marek</creatorcontrib><creatorcontrib>Kordan, Władysław</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Animal reproduction science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kamiński, Stanisław</au><au>Hering, Dorota M.</au><au>Oleński, Kamil</au><au>Lecewicz, Marek</au><au>Kordan, Władysław</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genome-wide association study for sperm membrane integrity in frozen-thawed semen of Holstein-Friesian bulls</atitle><jtitle>Animal reproduction science</jtitle><addtitle>Anim Reprod Sci</addtitle><date>2016-07</date><risdate>2016</risdate><volume>170</volume><spage>135</spage><epage>140</epage><pages>135-140</pages><issn>0378-4320</issn><eissn>1873-2232</eissn><abstract>•First genome-wise association study for sperm membrane integrity in Holstein bulls.•SNP on chromosome 6 significantly associated with sperm plasma membrane integrity.•Three candidate genes involved in sperm plasma membrane integrity in thawed semen.
The aim of the study was to screen the entire bull genome to identify SNP markers and propose candidate genes potentially involved in the variation of sperm membrane integrity in Holstein-Friesian bulls. Two hundred eighty eight bulls kept in one AI center were included in the study. Each bull was genotyped for 54.001 Single Nucleotide Polymorpisms (SNP) by the Illumina BovineSNP50 BeadChip. Commercial straws of frozen-thawed semen were used for the evaluation of sperm plasma membrane integrity (SYBR-14/PI staining) and sperm mitochondrial function (JC1/PI staining). An additive model for Linear Regression Analysis was applied to estimate the effect of SNP marker for sperm membrane integrity (by the use of GoldenHelix SVS7 software). Five significant markers (encompassing 2,2 MB region located on chromosome 6) for SYBR-14/PI were found. Among them one marker—rs41570391 passed Bonferroni correction test. Within approximately 3 Mb genomic region including significant markers three candidate genes: SGMS2 (Sphingomyelin Synthase 2), TET2 (Methylcytosine dioxygenase 2) and GSTCD genes (Gluthatione S-transferase C terminal domain) were proposed as potentially involved in sperm membrane integrity in frozen-thawed semen of Holstein-Friesian bulls.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>27236378</pmid><doi>10.1016/j.anireprosci.2016.05.002</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0002-5538-1352</orcidid></addata></record> |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Bull Cattle Cell Membrane - physiology Cryopreservation - veterinary Freezing Gene Expression Regulation Genome-wide association study Genome-Wide Association Study - veterinary Genotype Male Polymorphism, Single Nucleotide Semen Preservation - veterinary SNP Sperm membrane |
| title | Genome-wide association study for sperm membrane integrity in frozen-thawed semen of Holstein-Friesian bulls |
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