Evaluation of a transcription mediated amplification assay for detection of herpes simplex virus types 1 and 2 mRNA in clinical specimens
Highlights • Herpes simplex viruses (HSVs) are double-stranded DNA viruses that package viral mRNAs. • A real time amplified molecular test was designed to detect packaged viral mRNA. • This test exhibited high clinical sensitivity and specificity in an STD clinic population.
Gespeichert in:
| Veröffentlicht in: | Journal of clinical virology 2016-07, Vol.80, p.62-67 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 67 |
|---|---|
| container_issue | |
| container_start_page | 62 |
| container_title | Journal of clinical virology |
| container_volume | 80 |
| creator | Swenson, Paul D El-Sabaeny, Azza Thomas-Moricz, Vanessa Allen, Megan Groskopf, Anabel Jiang, Alice Getman, Damon |
| description | Highlights • Herpes simplex viruses (HSVs) are double-stranded DNA viruses that package viral mRNAs. • A real time amplified molecular test was designed to detect packaged viral mRNA. • This test exhibited high clinical sensitivity and specificity in an STD clinic population. |
| doi_str_mv | 10.1016/j.jcv.2016.04.023 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1808634480</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1386653216301019</els_id><sourcerecordid>1795870875</sourcerecordid><originalsourceid>FETCH-LOGICAL-c484t-ddc7d9ad370eef8562ec58af881c2ed3a7f0b764d06adb79abdbd6b67180106f3</originalsourceid><addsrcrecordid>eNqFks1u1TAQhS0Eoj_wAGyQl2wS7DixHSEhVVWhSBWVCqwtx54Ih_zhSa64j8Bb43B7u-gCVjMafecszhlCXnGWc8bl2y7v3C4v0pqzMmeFeEJOuVYiq2qpnqZdaJnJShQn5AyxY4xXolTPyUmhuKpKpU_J76ud7Ve7hGmkU0stXaId0cUw_z0N4INdwFM7zH1ogzuQFtHuaTtF6mEBd1R_hzgDUgwJhl90F-KKdNlvN07t6GlBh7vPFzSM1PVhTG49xRlcGGDEF-RZa3uEl_fznHz7cPX18jq7uf346fLiJnOlLpfMe6d8bb1QDKDVlSzAVdq2WnNXgBdWtaxRsvRMWt-o2ja-8bKRimvGmWzFOXlz8J3j9HMFXMwQ0EHf2xGmFU3itBRlqdn_UVVXWjGtqoTyA-rihBihNXMMg417w5nZyjKdSWWZrSzDSpPKSprX9_Zrk4J-UBzbScC7AwApj12AaNAFGF0qJabUjZ_CP-3fP1IfQ_8Be8BuWuOYgjbcYGGY-bJ9y_YsXIqUFK_FHxS9vB8</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1795870875</pqid></control><display><type>article</type><title>Evaluation of a transcription mediated amplification assay for detection of herpes simplex virus types 1 and 2 mRNA in clinical specimens</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Swenson, Paul D ; El-Sabaeny, Azza ; Thomas-Moricz, Vanessa ; Allen, Megan ; Groskopf, Anabel ; Jiang, Alice ; Getman, Damon</creator><creatorcontrib>Swenson, Paul D ; El-Sabaeny, Azza ; Thomas-Moricz, Vanessa ; Allen, Megan ; Groskopf, Anabel ; Jiang, Alice ; Getman, Damon</creatorcontrib><description>Highlights • Herpes simplex viruses (HSVs) are double-stranded DNA viruses that package viral mRNAs. • A real time amplified molecular test was designed to detect packaged viral mRNA. • This test exhibited high clinical sensitivity and specificity in an STD clinic population.</description><identifier>ISSN: 1386-6532</identifier><identifier>EISSN: 1873-5967</identifier><identifier>DOI: 10.1016/j.jcv.2016.04.023</identifier><identifier>PMID: 27175478</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Allergy and Immunology ; Aptima ; Cytomegalovirus ; Female ; Herpes Simplex - diagnosis ; Herpes simplex virus (HSV) ; Herpes simplex virus 1 ; Herpes simplex virus 2 ; Herpesvirus 1, Human - genetics ; Herpesvirus 1, Human - growth & development ; Herpesvirus 2, Human - genetics ; Herpesvirus 2, Human - growth & development ; Human herpesvirus 3 ; Human herpesvirus 4 ; Human herpesvirus 5 ; Human herpesvirus 6 ; Human herpesvirus 8 ; Humans ; Infectious Disease ; Male ; Real-Time Polymerase Chain Reaction ; RNA, Messenger - analysis ; RNA, Viral - analysis ; Sensitivity and Specificity ; Transcription, Genetic ; Transcription-mediated amplification ; UL42 mRNA</subject><ispartof>Journal of clinical virology, 2016-07, Vol.80, p.62-67</ispartof><rights>2016 The Author(s)</rights><rights>Copyright © 2016 The Author(s). Published by Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c484t-ddc7d9ad370eef8562ec58af881c2ed3a7f0b764d06adb79abdbd6b67180106f3</citedby><cites>FETCH-LOGICAL-c484t-ddc7d9ad370eef8562ec58af881c2ed3a7f0b764d06adb79abdbd6b67180106f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jcv.2016.04.023$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27175478$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Swenson, Paul D</creatorcontrib><creatorcontrib>El-Sabaeny, Azza</creatorcontrib><creatorcontrib>Thomas-Moricz, Vanessa</creatorcontrib><creatorcontrib>Allen, Megan</creatorcontrib><creatorcontrib>Groskopf, Anabel</creatorcontrib><creatorcontrib>Jiang, Alice</creatorcontrib><creatorcontrib>Getman, Damon</creatorcontrib><title>Evaluation of a transcription mediated amplification assay for detection of herpes simplex virus types 1 and 2 mRNA in clinical specimens</title><title>Journal of clinical virology</title><addtitle>J Clin Virol</addtitle><description>Highlights • Herpes simplex viruses (HSVs) are double-stranded DNA viruses that package viral mRNAs. • A real time amplified molecular test was designed to detect packaged viral mRNA. • This test exhibited high clinical sensitivity and specificity in an STD clinic population.</description><subject>Allergy and Immunology</subject><subject>Aptima</subject><subject>Cytomegalovirus</subject><subject>Female</subject><subject>Herpes Simplex - diagnosis</subject><subject>Herpes simplex virus (HSV)</subject><subject>Herpes simplex virus 1</subject><subject>Herpes simplex virus 2</subject><subject>Herpesvirus 1, Human - genetics</subject><subject>Herpesvirus 1, Human - growth & development</subject><subject>Herpesvirus 2, Human - genetics</subject><subject>Herpesvirus 2, Human - growth & development</subject><subject>Human herpesvirus 3</subject><subject>Human herpesvirus 4</subject><subject>Human herpesvirus 5</subject><subject>Human herpesvirus 6</subject><subject>Human herpesvirus 8</subject><subject>Humans</subject><subject>Infectious Disease</subject><subject>Male</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>RNA, Messenger - analysis</subject><subject>RNA, Viral - analysis</subject><subject>Sensitivity and Specificity</subject><subject>Transcription, Genetic</subject><subject>Transcription-mediated amplification</subject><subject>UL42 mRNA</subject><issn>1386-6532</issn><issn>1873-5967</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks1u1TAQhS0Eoj_wAGyQl2wS7DixHSEhVVWhSBWVCqwtx54Ih_zhSa64j8Bb43B7u-gCVjMafecszhlCXnGWc8bl2y7v3C4v0pqzMmeFeEJOuVYiq2qpnqZdaJnJShQn5AyxY4xXolTPyUmhuKpKpU_J76ud7Ve7hGmkU0stXaId0cUw_z0N4INdwFM7zH1ogzuQFtHuaTtF6mEBd1R_hzgDUgwJhl90F-KKdNlvN07t6GlBh7vPFzSM1PVhTG49xRlcGGDEF-RZa3uEl_fznHz7cPX18jq7uf346fLiJnOlLpfMe6d8bb1QDKDVlSzAVdq2WnNXgBdWtaxRsvRMWt-o2ja-8bKRimvGmWzFOXlz8J3j9HMFXMwQ0EHf2xGmFU3itBRlqdn_UVVXWjGtqoTyA-rihBihNXMMg417w5nZyjKdSWWZrSzDSpPKSprX9_Zrk4J-UBzbScC7AwApj12AaNAFGF0qJabUjZ_CP-3fP1IfQ_8Be8BuWuOYgjbcYGGY-bJ9y_YsXIqUFK_FHxS9vB8</recordid><startdate>20160701</startdate><enddate>20160701</enddate><creator>Swenson, Paul D</creator><creator>El-Sabaeny, Azza</creator><creator>Thomas-Moricz, Vanessa</creator><creator>Allen, Megan</creator><creator>Groskopf, Anabel</creator><creator>Jiang, Alice</creator><creator>Getman, Damon</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TM</scope><scope>7U9</scope><scope>H94</scope></search><sort><creationdate>20160701</creationdate><title>Evaluation of a transcription mediated amplification assay for detection of herpes simplex virus types 1 and 2 mRNA in clinical specimens</title><author>Swenson, Paul D ; El-Sabaeny, Azza ; Thomas-Moricz, Vanessa ; Allen, Megan ; Groskopf, Anabel ; Jiang, Alice ; Getman, Damon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c484t-ddc7d9ad370eef8562ec58af881c2ed3a7f0b764d06adb79abdbd6b67180106f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Allergy and Immunology</topic><topic>Aptima</topic><topic>Cytomegalovirus</topic><topic>Female</topic><topic>Herpes Simplex - diagnosis</topic><topic>Herpes simplex virus (HSV)</topic><topic>Herpes simplex virus 1</topic><topic>Herpes simplex virus 2</topic><topic>Herpesvirus 1, Human - genetics</topic><topic>Herpesvirus 1, Human - growth & development</topic><topic>Herpesvirus 2, Human - genetics</topic><topic>Herpesvirus 2, Human - growth & development</topic><topic>Human herpesvirus 3</topic><topic>Human herpesvirus 4</topic><topic>Human herpesvirus 5</topic><topic>Human herpesvirus 6</topic><topic>Human herpesvirus 8</topic><topic>Humans</topic><topic>Infectious Disease</topic><topic>Male</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>RNA, Messenger - analysis</topic><topic>RNA, Viral - analysis</topic><topic>Sensitivity and Specificity</topic><topic>Transcription, Genetic</topic><topic>Transcription-mediated amplification</topic><topic>UL42 mRNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Swenson, Paul D</creatorcontrib><creatorcontrib>El-Sabaeny, Azza</creatorcontrib><creatorcontrib>Thomas-Moricz, Vanessa</creatorcontrib><creatorcontrib>Allen, Megan</creatorcontrib><creatorcontrib>Groskopf, Anabel</creatorcontrib><creatorcontrib>Jiang, Alice</creatorcontrib><creatorcontrib>Getman, Damon</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Journal of clinical virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Swenson, Paul D</au><au>El-Sabaeny, Azza</au><au>Thomas-Moricz, Vanessa</au><au>Allen, Megan</au><au>Groskopf, Anabel</au><au>Jiang, Alice</au><au>Getman, Damon</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of a transcription mediated amplification assay for detection of herpes simplex virus types 1 and 2 mRNA in clinical specimens</atitle><jtitle>Journal of clinical virology</jtitle><addtitle>J Clin Virol</addtitle><date>2016-07-01</date><risdate>2016</risdate><volume>80</volume><spage>62</spage><epage>67</epage><pages>62-67</pages><issn>1386-6532</issn><eissn>1873-5967</eissn><abstract>Highlights • Herpes simplex viruses (HSVs) are double-stranded DNA viruses that package viral mRNAs. • A real time amplified molecular test was designed to detect packaged viral mRNA. • This test exhibited high clinical sensitivity and specificity in an STD clinic population.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>27175478</pmid><doi>10.1016/j.jcv.2016.04.023</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1386-6532 |
| ispartof | Journal of clinical virology, 2016-07, Vol.80, p.62-67 |
| issn | 1386-6532 1873-5967 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1808634480 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Allergy and Immunology Aptima Cytomegalovirus Female Herpes Simplex - diagnosis Herpes simplex virus (HSV) Herpes simplex virus 1 Herpes simplex virus 2 Herpesvirus 1, Human - genetics Herpesvirus 1, Human - growth & development Herpesvirus 2, Human - genetics Herpesvirus 2, Human - growth & development Human herpesvirus 3 Human herpesvirus 4 Human herpesvirus 5 Human herpesvirus 6 Human herpesvirus 8 Humans Infectious Disease Male Real-Time Polymerase Chain Reaction RNA, Messenger - analysis RNA, Viral - analysis Sensitivity and Specificity Transcription, Genetic Transcription-mediated amplification UL42 mRNA |
| title | Evaluation of a transcription mediated amplification assay for detection of herpes simplex virus types 1 and 2 mRNA in clinical specimens |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-10T14%3A49%3A27IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Evaluation%20of%20a%20transcription%20mediated%20amplification%20assay%20for%20detection%20of%20herpes%20simplex%20virus%20types%201%20and%202%20mRNA%20in%20clinical%20specimens&rft.jtitle=Journal%20of%20clinical%20virology&rft.au=Swenson,%20Paul%20D&rft.date=2016-07-01&rft.volume=80&rft.spage=62&rft.epage=67&rft.pages=62-67&rft.issn=1386-6532&rft.eissn=1873-5967&rft_id=info:doi/10.1016/j.jcv.2016.04.023&rft_dat=%3Cproquest_cross%3E1795870875%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1795870875&rft_id=info:pmid/27175478&rft_els_id=S1386653216301019&rfr_iscdi=true |