The synergistic effect of nano-hydroxyapatite and dexamethasone in the fibrous delivery system of gelatin and poly(l-lactide) on the osteogenesis of mesenchymal stem cells
[Display omitted] Recently, electrospun nanofibrous scaffolds are vastly taken into consideration in the bone tissue engineering due to mimicking the natural structure of native tissue. In our study, surface features of nanofibers were modified through simultaneous electrospining of the synthetic an...
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| Veröffentlicht in: | International journal of pharmaceutics 2016-06, Vol.507 (1-2), p.1-11 |
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| creator | Amjadian, Sara Seyedjafari, Ehsan Zeynali, Bahman Shabani, Iman |
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Recently, electrospun nanofibrous scaffolds are vastly taken into consideration in the bone tissue engineering due to mimicking the natural structure of native tissue. In our study, surface features of nanofibers were modified through simultaneous electrospining of the synthetic and natural polymers using poly l-lactide (PLLA) and gelatin to fabricate the hybrid scaffold (PLLA/gelatin). Then, hydroxyapatite nanoparticles (nHA) were loaded in electrospun PLLA nanofibers (PLLA,nHA/gelatin) and also dexamethasone (DEX) was incorporated in these fibers (PLLA,nHA,DEX/gelatin) in the second experiment. Fabricated nanofibrous composite scaffolds were characterized via SEM, FTIR spectroscopy, contact angle, tensile strength measurements, DEX release profile and MTT assay. After seeding adipose derived mesenchymal stem cells, osteoinductivity and osteoconductivity of fabricated scaffolds were analyzed using common osteogenic markers such as alkaline phosphatase activity, calcium depositions and gene expression. These results confirmed that all properties of nanofibers were improved by modifications. Moreover, osteogenic differentiation of stem cells increased in PLLA,nHA/gelatin group in comparison with PLLA/gelatin. The sustained release of DEX was obtained from PLLA,nHA,DEX/gelatin which subsequently led to more osteogenic differentiation. Taken together, PLLA,nHA,DEX/gelatin showed significant potential to support the stem cell proliferation and ostogenic differentiation, and can be a good candidates for tissue engineering and regenerative medicine applications. |
| doi_str_mv | 10.1016/j.ijpharm.2016.04.032 |
| format | Article |
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Recently, electrospun nanofibrous scaffolds are vastly taken into consideration in the bone tissue engineering due to mimicking the natural structure of native tissue. In our study, surface features of nanofibers were modified through simultaneous electrospining of the synthetic and natural polymers using poly l-lactide (PLLA) and gelatin to fabricate the hybrid scaffold (PLLA/gelatin). Then, hydroxyapatite nanoparticles (nHA) were loaded in electrospun PLLA nanofibers (PLLA,nHA/gelatin) and also dexamethasone (DEX) was incorporated in these fibers (PLLA,nHA,DEX/gelatin) in the second experiment. Fabricated nanofibrous composite scaffolds were characterized via SEM, FTIR spectroscopy, contact angle, tensile strength measurements, DEX release profile and MTT assay. After seeding adipose derived mesenchymal stem cells, osteoinductivity and osteoconductivity of fabricated scaffolds were analyzed using common osteogenic markers such as alkaline phosphatase activity, calcium depositions and gene expression. These results confirmed that all properties of nanofibers were improved by modifications. Moreover, osteogenic differentiation of stem cells increased in PLLA,nHA/gelatin group in comparison with PLLA/gelatin. The sustained release of DEX was obtained from PLLA,nHA,DEX/gelatin which subsequently led to more osteogenic differentiation. Taken together, PLLA,nHA,DEX/gelatin showed significant potential to support the stem cell proliferation and ostogenic differentiation, and can be a good candidates for tissue engineering and regenerative medicine applications.</description><identifier>ISSN: 0378-5173</identifier><identifier>EISSN: 1873-3476</identifier><identifier>DOI: 10.1016/j.ijpharm.2016.04.032</identifier><identifier>PMID: 27107902</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Cell Proliferation - drug effects ; Cells, Cultured ; Dexamethasone ; Dexamethasone - administration & dosage ; Dexamethasone - chemistry ; Dexamethasone - pharmacokinetics ; Dexamethasone - pharmacology ; Drug Delivery Systems ; Drug Liberation ; Drug Synergism ; Durapatite - administration & dosage ; Durapatite - chemistry ; Electrospinning ; Gelatin ; Gelatin - administration & dosage ; Gelatin - chemistry ; Humans ; Mesenchymal stem cells ; Mesenchymal Stromal Cells - cytology ; Mesenchymal Stromal Cells - drug effects ; Nano-hydroxyapatite ; Nanofibers - chemistry ; Nanofibers - ultrastructure ; Nanoparticles - chemistry ; Nanoparticles - ultrastructure ; Osteogenesis - drug effects ; Polyesters - administration & dosage ; Polyesters - chemistry ; Tensile Strength ; Tissue engineering</subject><ispartof>International journal of pharmaceutics, 2016-06, Vol.507 (1-2), p.1-11</ispartof><rights>2016 Elsevier B.V.</rights><rights>Copyright © 2016 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c464t-76e25ca25fe5820613e492781181ced4e2dc87f27d43eef1db136376a60332043</citedby><cites>FETCH-LOGICAL-c464t-76e25ca25fe5820613e492781181ced4e2dc87f27d43eef1db136376a60332043</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ijpharm.2016.04.032$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27107902$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Amjadian, Sara</creatorcontrib><creatorcontrib>Seyedjafari, Ehsan</creatorcontrib><creatorcontrib>Zeynali, Bahman</creatorcontrib><creatorcontrib>Shabani, Iman</creatorcontrib><title>The synergistic effect of nano-hydroxyapatite and dexamethasone in the fibrous delivery system of gelatin and poly(l-lactide) on the osteogenesis of mesenchymal stem cells</title><title>International journal of pharmaceutics</title><addtitle>Int J Pharm</addtitle><description>[Display omitted]
Recently, electrospun nanofibrous scaffolds are vastly taken into consideration in the bone tissue engineering due to mimicking the natural structure of native tissue. In our study, surface features of nanofibers were modified through simultaneous electrospining of the synthetic and natural polymers using poly l-lactide (PLLA) and gelatin to fabricate the hybrid scaffold (PLLA/gelatin). Then, hydroxyapatite nanoparticles (nHA) were loaded in electrospun PLLA nanofibers (PLLA,nHA/gelatin) and also dexamethasone (DEX) was incorporated in these fibers (PLLA,nHA,DEX/gelatin) in the second experiment. Fabricated nanofibrous composite scaffolds were characterized via SEM, FTIR spectroscopy, contact angle, tensile strength measurements, DEX release profile and MTT assay. After seeding adipose derived mesenchymal stem cells, osteoinductivity and osteoconductivity of fabricated scaffolds were analyzed using common osteogenic markers such as alkaline phosphatase activity, calcium depositions and gene expression. These results confirmed that all properties of nanofibers were improved by modifications. Moreover, osteogenic differentiation of stem cells increased in PLLA,nHA/gelatin group in comparison with PLLA/gelatin. The sustained release of DEX was obtained from PLLA,nHA,DEX/gelatin which subsequently led to more osteogenic differentiation. Taken together, PLLA,nHA,DEX/gelatin showed significant potential to support the stem cell proliferation and ostogenic differentiation, and can be a good candidates for tissue engineering and regenerative medicine applications.</description><subject>Cell Proliferation - drug effects</subject><subject>Cells, Cultured</subject><subject>Dexamethasone</subject><subject>Dexamethasone - administration & dosage</subject><subject>Dexamethasone - chemistry</subject><subject>Dexamethasone - pharmacokinetics</subject><subject>Dexamethasone - pharmacology</subject><subject>Drug Delivery Systems</subject><subject>Drug Liberation</subject><subject>Drug Synergism</subject><subject>Durapatite - administration & dosage</subject><subject>Durapatite - chemistry</subject><subject>Electrospinning</subject><subject>Gelatin</subject><subject>Gelatin - administration & dosage</subject><subject>Gelatin - chemistry</subject><subject>Humans</subject><subject>Mesenchymal stem cells</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Mesenchymal Stromal Cells - drug effects</subject><subject>Nano-hydroxyapatite</subject><subject>Nanofibers - chemistry</subject><subject>Nanofibers - ultrastructure</subject><subject>Nanoparticles - chemistry</subject><subject>Nanoparticles - ultrastructure</subject><subject>Osteogenesis - drug effects</subject><subject>Polyesters - administration & dosage</subject><subject>Polyesters - chemistry</subject><subject>Tensile Strength</subject><subject>Tissue engineering</subject><issn>0378-5173</issn><issn>1873-3476</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1u1DAUhS0EokPhEUBelkWCfxI7WSFUFYpUiU1ZWx77ZuJRYg-2p2qeqS-J0xnYwsqyfM651-dD6D0lNSVUfNrXbn8YdZxrVq41aWrC2Qu0oZ3kFW-keIk2hMuuaqnkF-hNSntCiGCUv0YXTFIie8I26Ol-BJwWD3HnUnYGwzCAyTgM2GsfqnGxMTwu-qCzy4C1t9jCo54hjzoFD9h5nEvE4LYxHFN5nNwDxKVkpgzzmrODqZj9s_cQpuVqqiZtsrPwEYeTOxRt2IGH5NJqmSGBN-My6wk_xxiYpvQWvRr0lODd-bxEP7_e3F_fVnc_vn2__nJXmUY0uZICWGs0awdoO0YE5dD0THaUdtSAbYBZ08mBSdtwgIHaLeWCS6EF4ZyRhl-iq1PuIYZfR0hZzS6tG2gP5Y-KdqQTtO_7_5DKnpfKCe2KtD1JTQwpRRjUIbpZx0VRolakaq_OSNWKVJFGFaTF9-E84ridwf51_WFYBJ9PAiidPDiIKhlX6gPrYkGpbHD_GPEbBAK4DQ</recordid><startdate>20160630</startdate><enddate>20160630</enddate><creator>Amjadian, Sara</creator><creator>Seyedjafari, Ehsan</creator><creator>Zeynali, Bahman</creator><creator>Shabani, Iman</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>7QP</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20160630</creationdate><title>The synergistic effect of nano-hydroxyapatite and dexamethasone in the fibrous delivery system of gelatin and poly(l-lactide) on the osteogenesis of mesenchymal stem cells</title><author>Amjadian, Sara ; Seyedjafari, Ehsan ; Zeynali, Bahman ; Shabani, Iman</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c464t-76e25ca25fe5820613e492781181ced4e2dc87f27d43eef1db136376a60332043</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Cell Proliferation - drug effects</topic><topic>Cells, Cultured</topic><topic>Dexamethasone</topic><topic>Dexamethasone - administration & dosage</topic><topic>Dexamethasone - chemistry</topic><topic>Dexamethasone - pharmacokinetics</topic><topic>Dexamethasone - pharmacology</topic><topic>Drug Delivery Systems</topic><topic>Drug Liberation</topic><topic>Drug Synergism</topic><topic>Durapatite - administration & dosage</topic><topic>Durapatite - chemistry</topic><topic>Electrospinning</topic><topic>Gelatin</topic><topic>Gelatin - administration & dosage</topic><topic>Gelatin - chemistry</topic><topic>Humans</topic><topic>Mesenchymal stem cells</topic><topic>Mesenchymal Stromal Cells - cytology</topic><topic>Mesenchymal Stromal Cells - drug effects</topic><topic>Nano-hydroxyapatite</topic><topic>Nanofibers - chemistry</topic><topic>Nanofibers - ultrastructure</topic><topic>Nanoparticles - chemistry</topic><topic>Nanoparticles - ultrastructure</topic><topic>Osteogenesis - drug effects</topic><topic>Polyesters - administration & dosage</topic><topic>Polyesters - chemistry</topic><topic>Tensile Strength</topic><topic>Tissue engineering</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Amjadian, Sara</creatorcontrib><creatorcontrib>Seyedjafari, Ehsan</creatorcontrib><creatorcontrib>Zeynali, Bahman</creatorcontrib><creatorcontrib>Shabani, Iman</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>International journal of pharmaceutics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Amjadian, Sara</au><au>Seyedjafari, Ehsan</au><au>Zeynali, Bahman</au><au>Shabani, Iman</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The synergistic effect of nano-hydroxyapatite and dexamethasone in the fibrous delivery system of gelatin and poly(l-lactide) on the osteogenesis of mesenchymal stem cells</atitle><jtitle>International journal of pharmaceutics</jtitle><addtitle>Int J Pharm</addtitle><date>2016-06-30</date><risdate>2016</risdate><volume>507</volume><issue>1-2</issue><spage>1</spage><epage>11</epage><pages>1-11</pages><issn>0378-5173</issn><eissn>1873-3476</eissn><abstract>[Display omitted]
Recently, electrospun nanofibrous scaffolds are vastly taken into consideration in the bone tissue engineering due to mimicking the natural structure of native tissue. In our study, surface features of nanofibers were modified through simultaneous electrospining of the synthetic and natural polymers using poly l-lactide (PLLA) and gelatin to fabricate the hybrid scaffold (PLLA/gelatin). Then, hydroxyapatite nanoparticles (nHA) were loaded in electrospun PLLA nanofibers (PLLA,nHA/gelatin) and also dexamethasone (DEX) was incorporated in these fibers (PLLA,nHA,DEX/gelatin) in the second experiment. Fabricated nanofibrous composite scaffolds were characterized via SEM, FTIR spectroscopy, contact angle, tensile strength measurements, DEX release profile and MTT assay. After seeding adipose derived mesenchymal stem cells, osteoinductivity and osteoconductivity of fabricated scaffolds were analyzed using common osteogenic markers such as alkaline phosphatase activity, calcium depositions and gene expression. These results confirmed that all properties of nanofibers were improved by modifications. Moreover, osteogenic differentiation of stem cells increased in PLLA,nHA/gelatin group in comparison with PLLA/gelatin. The sustained release of DEX was obtained from PLLA,nHA,DEX/gelatin which subsequently led to more osteogenic differentiation. Taken together, PLLA,nHA,DEX/gelatin showed significant potential to support the stem cell proliferation and ostogenic differentiation, and can be a good candidates for tissue engineering and regenerative medicine applications.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>27107902</pmid><doi>10.1016/j.ijpharm.2016.04.032</doi><tpages>11</tpages></addata></record> |
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| subjects | Cell Proliferation - drug effects Cells, Cultured Dexamethasone Dexamethasone - administration & dosage Dexamethasone - chemistry Dexamethasone - pharmacokinetics Dexamethasone - pharmacology Drug Delivery Systems Drug Liberation Drug Synergism Durapatite - administration & dosage Durapatite - chemistry Electrospinning Gelatin Gelatin - administration & dosage Gelatin - chemistry Humans Mesenchymal stem cells Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - drug effects Nano-hydroxyapatite Nanofibers - chemistry Nanofibers - ultrastructure Nanoparticles - chemistry Nanoparticles - ultrastructure Osteogenesis - drug effects Polyesters - administration & dosage Polyesters - chemistry Tensile Strength Tissue engineering |
| title | The synergistic effect of nano-hydroxyapatite and dexamethasone in the fibrous delivery system of gelatin and poly(l-lactide) on the osteogenesis of mesenchymal stem cells |
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