Features for instantaneous emissions of low-level infrared signals of glucokinase enzyme from Pyrococcus furiosus
A noncontact infrared (IR) imaging-based methodology and signal recovery tools are applied on an enzyme reaction as a test target. The method is implemented by a long-wave (8-12 μm) IR microbolometer imaging array and a germanium-based IR optical vision. The reaction is carried out by the glucokinas...
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| Veröffentlicht in: | Applied optics (2004) 2015-03, Vol.54 (8), p.2057-2065 |
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| container_issue | 8 |
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| container_title | Applied optics (2004) |
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| creator | Torres, Sergio Mella, Héctor Reyes, Claudio Meza, Pablo Gallardo, Maria J Staforelli, Juan P |
| description | A noncontact infrared (IR) imaging-based methodology and signal recovery tools are applied on an enzyme reaction as a test target. The method is implemented by a long-wave (8-12 μm) IR microbolometer imaging array and a germanium-based IR optical vision. The reaction is carried out by the glucokinase, which produces a rapid exothermal release of energy that is weak, and, even worse, the IR video captured by the uncooled microbolometer detector is affected by spatial and temporal noise with specific complexities. Hitherto, IR-based signal recovery tools have worked with a standard acquisition frequency, which is clearly beyond the time scale of a real scenario. The implications of this (and similar) rapid reactions motivate the designs of a signal recovery method using prior information of the processes to extract and quantify the spontaneity of the enzymatic reaction in a three-dimensional (space and time) single and noncontact online measurement. |
| doi_str_mv | 10.1364/AO.54.002057 |
| format | Article |
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| identifier | ISSN: 1559-128X |
| ispartof | Applied optics (2004), 2015-03, Vol.54 (8), p.2057-2065 |
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| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection; Optica Publishing Group Journals |
| subjects | Adenosine Triphosphate - chemistry Arrays Calibration Detectors Electronics Enzymes Equipment Design Glucokinase - chemistry Hot Temperature Infrared Infrared imaging Infrared Rays Normal Distribution Optical Devices Optical Phenomena Pyrococcus furiosus Pyrococcus furiosus - enzymology Recovery Spectrophotometry, Infrared - instrumentation Spectrophotometry, Infrared - methods Three dimensional |
| title | Features for instantaneous emissions of low-level infrared signals of glucokinase enzyme from Pyrococcus furiosus |
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