Anti-Toxoplasma activity and impact evaluation of lyophilization, hot molding process, and gamma-irradiation techniques on CLH-PLGA intravitreal implants
Intraocular delivery systems have been developed to treat many eye diseases, especially those affecting the posterior segment of the eye. However, ocular toxoplasmosis, the leading cause of infectious posterior uveitis in the world, still lacks an effective treatment. Therefore, our group developed...
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| Veröffentlicht in: | Journal of materials science. Materials in medicine 2016, Vol.27 (1), p.10-12, Article 10 |
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| creator | Fernandes-Cunha, Gabriella M. Rezende, Cíntia M. F. Mussel, Wagner N. da Silva, Gisele R. de L. Gomes, Elionai C. Yoshida, Maria I. Fialho, Sílvia L. Goes, Alfredo M. Gomes, Dawison A. de Almeida Vitor, Ricardo W. Silva-Cunha, Armando |
| description | Intraocular delivery systems have been developed to treat many eye diseases, especially those affecting the posterior segment of the eye. However, ocular toxoplasmosis, the leading cause of infectious posterior uveitis in the world, still lacks an effective treatment. Therefore, our group developed an intravitreal polymeric implant to release clindamycin, a potent anti-
Toxoplasma
antibiotic. In this work, we used different techniques such as differential scanning calorimetry, thermogravimetry, X-ray diffraction, scanning electron microscopy, and fourier-transform infrared spectroscopy to investigate drug/polymer properties while manufacturing the delivery system. We showed that the lyophilization, hot molding process, and sterilization by gamma irradiation did not change drug/polymer physical-chemistry properties. The drug was found to be homogeneously dispersed into the poly lactic-co-glycolic acid (PLGA) chains and the profile release was characterized by an initial burst followed by prolonged release. The drug profile release was not modified after gamma irradiation and non-covalent interaction was found between the drug and the PLGA. We also observed the preservation of the drug activity by showing the potent anti-
Toxoplasma
effect of the implant, after 24–72 h in contact with cells infected by the parasite, which highlights this system as an alternative to treat toxoplasmic retinochoroiditis.
Graphical Abstract |
| doi_str_mv | 10.1007/s10856-015-5621-1 |
| format | Article |
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Toxoplasma
antibiotic. In this work, we used different techniques such as differential scanning calorimetry, thermogravimetry, X-ray diffraction, scanning electron microscopy, and fourier-transform infrared spectroscopy to investigate drug/polymer properties while manufacturing the delivery system. We showed that the lyophilization, hot molding process, and sterilization by gamma irradiation did not change drug/polymer physical-chemistry properties. The drug was found to be homogeneously dispersed into the poly lactic-co-glycolic acid (PLGA) chains and the profile release was characterized by an initial burst followed by prolonged release. The drug profile release was not modified after gamma irradiation and non-covalent interaction was found between the drug and the PLGA. We also observed the preservation of the drug activity by showing the potent anti-
Toxoplasma
effect of the implant, after 24–72 h in contact with cells infected by the parasite, which highlights this system as an alternative to treat toxoplasmic retinochoroiditis.
Graphical Abstract</description><identifier>ISSN: 0957-4530</identifier><identifier>EISSN: 1573-4838</identifier><identifier>DOI: 10.1007/s10856-015-5621-1</identifier><identifier>PMID: 26676856</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Antiprotozoal Agents - administration & dosage ; Biomaterials ; Biomaterials Synthesis and Characterization ; Biomedical Engineering and Bioengineering ; Biomedical materials ; Calorimetry, Differential Scanning ; Cell Line ; Ceramics ; Chemistry and Materials Science ; Clindamycin - administration & dosage ; Composites ; Delivery systems ; Drug delivery systems ; Drugs ; Eyes ; Freeze Drying ; Gamma irradiation ; Gamma Rays ; Glass ; Hot Temperature ; Humans ; Implants ; Lactic Acid ; Materials Science ; Microscopy, Electron, Scanning ; Molding (process) ; Natural Materials ; Polyglycolic Acid ; Polymer Sciences ; Regenerative Medicine/Tissue Engineering ; Spectroscopy, Fourier Transform Infrared ; Surfaces and Interfaces ; Thermogravimetry ; Thin Films ; Toxoplasma - drug effects ; Vitreous Body ; X-Ray Diffraction</subject><ispartof>Journal of materials science. Materials in medicine, 2016, Vol.27 (1), p.10-12, Article 10</ispartof><rights>Springer Science+Business Media New York 2015</rights><rights>Springer Science+Business Media New York 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-d7059a3ee60ea3ad9fd968bcd7aab8244aac6abb2f44314d58b983db803ea11e3</citedby><cites>FETCH-LOGICAL-c438t-d7059a3ee60ea3ad9fd968bcd7aab8244aac6abb2f44314d58b983db803ea11e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10856-015-5621-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10856-015-5621-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,781,785,27929,27930,41493,42562,51324</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26676856$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fernandes-Cunha, Gabriella M.</creatorcontrib><creatorcontrib>Rezende, Cíntia M. F.</creatorcontrib><creatorcontrib>Mussel, Wagner N.</creatorcontrib><creatorcontrib>da Silva, Gisele R.</creatorcontrib><creatorcontrib>de L. Gomes, Elionai C.</creatorcontrib><creatorcontrib>Yoshida, Maria I.</creatorcontrib><creatorcontrib>Fialho, Sílvia L.</creatorcontrib><creatorcontrib>Goes, Alfredo M.</creatorcontrib><creatorcontrib>Gomes, Dawison A.</creatorcontrib><creatorcontrib>de Almeida Vitor, Ricardo W.</creatorcontrib><creatorcontrib>Silva-Cunha, Armando</creatorcontrib><title>Anti-Toxoplasma activity and impact evaluation of lyophilization, hot molding process, and gamma-irradiation techniques on CLH-PLGA intravitreal implants</title><title>Journal of materials science. Materials in medicine</title><addtitle>J Mater Sci: Mater Med</addtitle><addtitle>J Mater Sci Mater Med</addtitle><description>Intraocular delivery systems have been developed to treat many eye diseases, especially those affecting the posterior segment of the eye. However, ocular toxoplasmosis, the leading cause of infectious posterior uveitis in the world, still lacks an effective treatment. Therefore, our group developed an intravitreal polymeric implant to release clindamycin, a potent anti-
Toxoplasma
antibiotic. In this work, we used different techniques such as differential scanning calorimetry, thermogravimetry, X-ray diffraction, scanning electron microscopy, and fourier-transform infrared spectroscopy to investigate drug/polymer properties while manufacturing the delivery system. We showed that the lyophilization, hot molding process, and sterilization by gamma irradiation did not change drug/polymer physical-chemistry properties. The drug was found to be homogeneously dispersed into the poly lactic-co-glycolic acid (PLGA) chains and the profile release was characterized by an initial burst followed by prolonged release. The drug profile release was not modified after gamma irradiation and non-covalent interaction was found between the drug and the PLGA. We also observed the preservation of the drug activity by showing the potent anti-
Toxoplasma
effect of the implant, after 24–72 h in contact with cells infected by the parasite, which highlights this system as an alternative to treat toxoplasmic retinochoroiditis.
Graphical Abstract</description><subject>Antiprotozoal Agents - administration & dosage</subject><subject>Biomaterials</subject><subject>Biomaterials Synthesis and Characterization</subject><subject>Biomedical Engineering and Bioengineering</subject><subject>Biomedical materials</subject><subject>Calorimetry, Differential Scanning</subject><subject>Cell Line</subject><subject>Ceramics</subject><subject>Chemistry and Materials Science</subject><subject>Clindamycin - administration & dosage</subject><subject>Composites</subject><subject>Delivery systems</subject><subject>Drug delivery systems</subject><subject>Drugs</subject><subject>Eyes</subject><subject>Freeze Drying</subject><subject>Gamma irradiation</subject><subject>Gamma Rays</subject><subject>Glass</subject><subject>Hot Temperature</subject><subject>Humans</subject><subject>Implants</subject><subject>Lactic Acid</subject><subject>Materials Science</subject><subject>Microscopy, Electron, Scanning</subject><subject>Molding (process)</subject><subject>Natural Materials</subject><subject>Polyglycolic Acid</subject><subject>Polymer Sciences</subject><subject>Regenerative Medicine/Tissue Engineering</subject><subject>Spectroscopy, Fourier Transform Infrared</subject><subject>Surfaces and Interfaces</subject><subject>Thermogravimetry</subject><subject>Thin Films</subject><subject>Toxoplasma - drug effects</subject><subject>Vitreous Body</subject><subject>X-Ray Diffraction</subject><issn>0957-4530</issn><issn>1573-4838</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNks1u1DAUhSMEokPhAdggS2xY1OD_OMvRCFqkkWBR1tFN7My4SuJgO1WHN-FtcZqCEBKClXXt75x7rHuL4iUlbykh5btIiZYKEyqxVIxi-qjYUFlyLDTXj4sNqWSJheTkrHgW4w0hRFRSPi3OmFKlytJN8X07Joev_Z2feogDIGiTu3XphGA0yA1TrpG9hX6G5PyIfIf6k5-Ornff7m8u0NEnNPjeuPGApuBbG-PFvfoAwwDYhQDGrepk2-Povs42olzt9lf48_5yi9yYAuSmwUK_9OxhTPF58aSDPtoXD-d58eXD--vdFd5_uvy42-5xK7hO2JREVsCtVcQCB1N1plK6aU0J0GgmBECroGlYJwSnwkjdVJqbRhNugVLLz4s3q2_OviRL9eBia_scwvo51lQTTRkjVflvtNREspIq_h-oJIIppmhGX_-B3vg5jPnPmVoGJnKETNGVaoOPMdiunoIbIJxqSuplG-p1G-q8DfWyDfXi_OrBeW4Ga34pfo4_A2wFYn4aDzb81vqvrj8ANXLByw</recordid><startdate>2016</startdate><enddate>2016</enddate><creator>Fernandes-Cunha, Gabriella M.</creator><creator>Rezende, Cíntia M. 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F. ; Mussel, Wagner N. ; da Silva, Gisele R. ; de L. Gomes, Elionai C. ; Yoshida, Maria I. ; Fialho, Sílvia L. ; Goes, Alfredo M. ; Gomes, Dawison A. ; de Almeida Vitor, Ricardo W. ; Silva-Cunha, Armando</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-d7059a3ee60ea3ad9fd968bcd7aab8244aac6abb2f44314d58b983db803ea11e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Antiprotozoal Agents - administration & dosage</topic><topic>Biomaterials</topic><topic>Biomaterials Synthesis and Characterization</topic><topic>Biomedical Engineering and Bioengineering</topic><topic>Biomedical materials</topic><topic>Calorimetry, Differential Scanning</topic><topic>Cell Line</topic><topic>Ceramics</topic><topic>Chemistry and Materials Science</topic><topic>Clindamycin - administration & dosage</topic><topic>Composites</topic><topic>Delivery systems</topic><topic>Drug delivery systems</topic><topic>Drugs</topic><topic>Eyes</topic><topic>Freeze Drying</topic><topic>Gamma irradiation</topic><topic>Gamma Rays</topic><topic>Glass</topic><topic>Hot Temperature</topic><topic>Humans</topic><topic>Implants</topic><topic>Lactic Acid</topic><topic>Materials Science</topic><topic>Microscopy, Electron, Scanning</topic><topic>Molding (process)</topic><topic>Natural Materials</topic><topic>Polyglycolic Acid</topic><topic>Polymer Sciences</topic><topic>Regenerative Medicine/Tissue Engineering</topic><topic>Spectroscopy, Fourier Transform Infrared</topic><topic>Surfaces and Interfaces</topic><topic>Thermogravimetry</topic><topic>Thin Films</topic><topic>Toxoplasma - drug effects</topic><topic>Vitreous Body</topic><topic>X-Ray Diffraction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fernandes-Cunha, Gabriella M.</creatorcontrib><creatorcontrib>Rezende, Cíntia M. 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Materials in medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fernandes-Cunha, Gabriella M.</au><au>Rezende, Cíntia M. F.</au><au>Mussel, Wagner N.</au><au>da Silva, Gisele R.</au><au>de L. Gomes, Elionai C.</au><au>Yoshida, Maria I.</au><au>Fialho, Sílvia L.</au><au>Goes, Alfredo M.</au><au>Gomes, Dawison A.</au><au>de Almeida Vitor, Ricardo W.</au><au>Silva-Cunha, Armando</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Anti-Toxoplasma activity and impact evaluation of lyophilization, hot molding process, and gamma-irradiation techniques on CLH-PLGA intravitreal implants</atitle><jtitle>Journal of materials science. Materials in medicine</jtitle><stitle>J Mater Sci: Mater Med</stitle><addtitle>J Mater Sci Mater Med</addtitle><date>2016</date><risdate>2016</risdate><volume>27</volume><issue>1</issue><spage>10</spage><epage>12</epage><pages>10-12</pages><artnum>10</artnum><issn>0957-4530</issn><eissn>1573-4838</eissn><abstract>Intraocular delivery systems have been developed to treat many eye diseases, especially those affecting the posterior segment of the eye. However, ocular toxoplasmosis, the leading cause of infectious posterior uveitis in the world, still lacks an effective treatment. Therefore, our group developed an intravitreal polymeric implant to release clindamycin, a potent anti-
Toxoplasma
antibiotic. In this work, we used different techniques such as differential scanning calorimetry, thermogravimetry, X-ray diffraction, scanning electron microscopy, and fourier-transform infrared spectroscopy to investigate drug/polymer properties while manufacturing the delivery system. We showed that the lyophilization, hot molding process, and sterilization by gamma irradiation did not change drug/polymer physical-chemistry properties. The drug was found to be homogeneously dispersed into the poly lactic-co-glycolic acid (PLGA) chains and the profile release was characterized by an initial burst followed by prolonged release. The drug profile release was not modified after gamma irradiation and non-covalent interaction was found between the drug and the PLGA. We also observed the preservation of the drug activity by showing the potent anti-
Toxoplasma
effect of the implant, after 24–72 h in contact with cells infected by the parasite, which highlights this system as an alternative to treat toxoplasmic retinochoroiditis.
Graphical Abstract</abstract><cop>New York</cop><pub>Springer US</pub><pmid>26676856</pmid><doi>10.1007/s10856-015-5621-1</doi><tpages>12</tpages></addata></record> |
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| ispartof | Journal of materials science. Materials in medicine, 2016, Vol.27 (1), p.10-12, Article 10 |
| issn | 0957-4530 1573-4838 |
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| subjects | Antiprotozoal Agents - administration & dosage Biomaterials Biomaterials Synthesis and Characterization Biomedical Engineering and Bioengineering Biomedical materials Calorimetry, Differential Scanning Cell Line Ceramics Chemistry and Materials Science Clindamycin - administration & dosage Composites Delivery systems Drug delivery systems Drugs Eyes Freeze Drying Gamma irradiation Gamma Rays Glass Hot Temperature Humans Implants Lactic Acid Materials Science Microscopy, Electron, Scanning Molding (process) Natural Materials Polyglycolic Acid Polymer Sciences Regenerative Medicine/Tissue Engineering Spectroscopy, Fourier Transform Infrared Surfaces and Interfaces Thermogravimetry Thin Films Toxoplasma - drug effects Vitreous Body X-Ray Diffraction |
| title | Anti-Toxoplasma activity and impact evaluation of lyophilization, hot molding process, and gamma-irradiation techniques on CLH-PLGA intravitreal implants |
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