Sequence Analysis and Heterologous Expression of Two Rhamnogalacturonan Acetylesterase Genes (Asrgae1 and Asrgaea2) from a Shoyu Koji Mold, Aspergillus sojae KBN1340
Two rhamnogalacturonan acetylesterase genes, designated as Asrgae1 and Asrgae2, were isolated from a shoyu koji mold, Aspergillus sojae KBN1340, and characterized. Asrgae1 comprised 793 bp, encoding 248 amino acids with a predicted molecular mass of 26,092 Da, interrupted by a single putative intron...
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| Veröffentlicht in: | FOOD SCIENCE AND TECHNOLOGY RESEARCH 2015, Vol.21(5), pp.665-670 |
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| creator | Kitamoto, Noriyuki Yoshino-Yasuda, Shoko |
| description | Two rhamnogalacturonan acetylesterase genes, designated as Asrgae1 and Asrgae2, were isolated from a shoyu koji mold, Aspergillus sojae KBN1340, and characterized. Asrgae1 comprised 793 bp, encoding 248 amino acids with a predicted molecular mass of 26,092 Da, interrupted by a single putative intron of 46 bp in length. The mature AsRgae1 of 232 amino acids had a calculated molecular mass of 24,415 Da. The coding region of Asrgae2 was determined to be 777 bp in length with no introns. The predicted protein of 258 amino acids had an estimated molecular mass of 26,902 Da. The mature AsRgae2 of 238 amino acids had a calculated molecular mass of 25,015 Da. Utilizing the A. oryzae taaG2 gene promoter and the A. oryzae taaG3 gene terminator, AsRgae1 and AsRgae2 were successfully expressed in A. oryzae and secreted into the culture medium. AsRgae1 and AsRgae2 had a molecular mass of 28.0 kDa and 33.0 kDa, respectively. |
| doi_str_mv | 10.3136/fstr.21.665 |
| format | Article |
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Asrgae1 comprised 793 bp, encoding 248 amino acids with a predicted molecular mass of 26,092 Da, interrupted by a single putative intron of 46 bp in length. The mature AsRgae1 of 232 amino acids had a calculated molecular mass of 24,415 Da. The coding region of Asrgae2 was determined to be 777 bp in length with no introns. The predicted protein of 258 amino acids had an estimated molecular mass of 26,902 Da. The mature AsRgae2 of 238 amino acids had a calculated molecular mass of 25,015 Da. Utilizing the A. oryzae taaG2 gene promoter and the A. oryzae taaG3 gene terminator, AsRgae1 and AsRgae2 were successfully expressed in A. oryzae and secreted into the culture medium. 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Asrgae1 comprised 793 bp, encoding 248 amino acids with a predicted molecular mass of 26,092 Da, interrupted by a single putative intron of 46 bp in length. The mature AsRgae1 of 232 amino acids had a calculated molecular mass of 24,415 Da. The coding region of Asrgae2 was determined to be 777 bp in length with no introns. The predicted protein of 258 amino acids had an estimated molecular mass of 26,902 Da. The mature AsRgae2 of 238 amino acids had a calculated molecular mass of 25,015 Da. Utilizing the A. oryzae taaG2 gene promoter and the A. oryzae taaG3 gene terminator, AsRgae1 and AsRgae2 were successfully expressed in A. oryzae and secreted into the culture medium. AsRgae1 and AsRgae2 had a molecular mass of 28.0 kDa and 33.0 kDa, respectively.</description><subject>Amino acids</subject><subject>Aspergillus</subject><subject>Aspergillus sojae</subject><subject>Culture</subject><subject>Gene expression</subject><subject>Genes</subject><subject>heterologous expression</subject><subject>Koji</subject><subject>Mathematical analysis</subject><subject>Molds</subject><subject>rhamnogalacturonan acetylesterase gene</subject><subject>shoyu koji mold</subject><issn>1344-6606</issn><issn>1881-3984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNqNkcFuEzEQhlcIJErhxAtY4lIEG2zvrnd9QQpVSVELSDR3a9YeJxt57WBvBHmgvmcdUgWJExfPSP7m_0fzF8VrRmcVq8QHm6Y442wmRPOkOGNdx8pKdvXT3Fd1XQpBxfPiRUobSlkjO35W3N_hzx16jWTuwe3TkAh4Q65xwhhcWIVdIle_txFTGoInwZLlr0B-rGH0YQUO9LSLwYMnc43T3mHKc5CQLNBjIhfzFFeA7I_msQf-ltgYRgLkbh32O3ITNgP5Gpx5n4ktxtXgXDZNYQNIbj59y5vTl8UzCy7hq8d6Xiw_Xy0vr8vb74svl_PbUouGTmWra2PblotcjNVSUst61lsAI_sKjYG2RcmtqTvsewNaNr1FY6UQrOZVdV5cHGW3MeSrpEmNQ9LoHHjMh1Csox1jjMr_QFvBq6bltM7om3_QTdjFfO0D1RwIVotMvTtSOoaUIlq1jcMIca8YVYdw1SFcxZnK4WZ6caRHNIMGF7wbPP4VtmNtQzBJ8Ry0opQzeii1onk6Py2tJBdcyKz08ai0SROs8OQKcRq0w5Nr82h9-tBriAp99QDVRcc7</recordid><startdate>2015</startdate><enddate>2015</enddate><creator>Kitamoto, Noriyuki</creator><creator>Yoshino-Yasuda, Shoko</creator><general>Japanese Society for Food Science and Technology</general><general>The Japanese Society for Food Science and Technology</general><general>Japan Science and Technology Agency</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QR</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>M7N</scope></search><sort><creationdate>2015</creationdate><title>Sequence Analysis and Heterologous Expression of Two Rhamnogalacturonan Acetylesterase Genes (Asrgae1 and Asrgaea2) from a Shoyu Koji Mold, Aspergillus sojae KBN1340</title><author>Kitamoto, Noriyuki ; Yoshino-Yasuda, Shoko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c650t-7c4df77264dfdfc990f1b1bfaad9b3edda77e92fd48ebbdac95bfedf96614233</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Amino acids</topic><topic>Aspergillus</topic><topic>Aspergillus sojae</topic><topic>Culture</topic><topic>Gene expression</topic><topic>Genes</topic><topic>heterologous expression</topic><topic>Koji</topic><topic>Mathematical analysis</topic><topic>Molds</topic><topic>rhamnogalacturonan acetylesterase gene</topic><topic>shoyu koji mold</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kitamoto, Noriyuki</creatorcontrib><creatorcontrib>Yoshino-Yasuda, Shoko</creatorcontrib><creatorcontrib>Food Research Center</creatorcontrib><creatorcontrib>Industrial Research Center</creatorcontrib><creatorcontrib>Aichi Center for Industry and Science Technology</creatorcontrib><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>FOOD SCIENCE AND TECHNOLOGY RESEARCH</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kitamoto, Noriyuki</au><au>Yoshino-Yasuda, Shoko</au><aucorp>Food Research Center</aucorp><aucorp>Industrial Research Center</aucorp><aucorp>Aichi Center for Industry and Science Technology</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sequence Analysis and Heterologous Expression of Two Rhamnogalacturonan Acetylesterase Genes (Asrgae1 and Asrgaea2) from a Shoyu Koji Mold, Aspergillus sojae KBN1340</atitle><jtitle>FOOD SCIENCE AND TECHNOLOGY RESEARCH</jtitle><addtitle>Food Science and Technology Research</addtitle><date>2015</date><risdate>2015</risdate><volume>21</volume><issue>5</issue><spage>665</spage><epage>670</epage><pages>665-670</pages><issn>1344-6606</issn><eissn>1881-3984</eissn><abstract>Two rhamnogalacturonan acetylesterase genes, designated as Asrgae1 and Asrgae2, were isolated from a shoyu koji mold, Aspergillus sojae KBN1340, and characterized. Asrgae1 comprised 793 bp, encoding 248 amino acids with a predicted molecular mass of 26,092 Da, interrupted by a single putative intron of 46 bp in length. The mature AsRgae1 of 232 amino acids had a calculated molecular mass of 24,415 Da. The coding region of Asrgae2 was determined to be 777 bp in length with no introns. The predicted protein of 258 amino acids had an estimated molecular mass of 26,902 Da. The mature AsRgae2 of 238 amino acids had a calculated molecular mass of 25,015 Da. Utilizing the A. oryzae taaG2 gene promoter and the A. oryzae taaG3 gene terminator, AsRgae1 and AsRgae2 were successfully expressed in A. oryzae and secreted into the culture medium. AsRgae1 and AsRgae2 had a molecular mass of 28.0 kDa and 33.0 kDa, respectively.</abstract><cop>Tsukuba</cop><pub>Japanese Society for Food Science and Technology</pub><doi>10.3136/fstr.21.665</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Amino acids Aspergillus Aspergillus sojae Culture Gene expression Genes heterologous expression Koji Mathematical analysis Molds rhamnogalacturonan acetylesterase gene shoyu koji mold |
| title | Sequence Analysis and Heterologous Expression of Two Rhamnogalacturonan Acetylesterase Genes (Asrgae1 and Asrgaea2) from a Shoyu Koji Mold, Aspergillus sojae KBN1340 |
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