A Radical Intermediate in Tyrosine Scission to the CO and CN⁻ Ligands of FeFe Hydrogenase

The radical S-adenosylmethionine (SAM) enzyme HydG lyses free L-tyrosine to produce CO and CN⁻ for the assembly of the catalytic H cluster of FeFe hydrogenase. We used electron paramagnetic resonance spectroscopy to detect and characterize HydG reaction intermediates generated with a set of ²H, ¹³C,...

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Veröffentlicht in:	Science (American Association for the Advancement of Science) 2013-10, Vol.342 (6157), p.472-475
Hauptverfasser:	Kuchenreuther, Jon M., Myers, William K., Stich, Troy A., George, Simon J., NejatyJahromy, Yaser, Swartz, James R., Britt, R. David
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino acids Atoms Bacterial Proteins - chemistry Bacterial Proteins - genetics Carbon Monoxide - chemistry Catalysis Catalytic Domain Chains Continuous spectra Electronic structure Electrons Enzymes Hydrogenase Hydrogenase - chemistry Iron-Sulfur Proteins - chemistry Iron-Sulfur Proteins - genetics Ligands Proteins Protons Radicals S-Adenosylmethionine - chemistry Scission Shewanella - enzymology Signals Spectroscopy Spectrum analysis Tyrosine Tyrosine - chemistry
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