A paper-based lateral flow assay for morphine
Morphine was used as a model analyte to examine the possibility of using cellulose, physically modified by papermaking and converting techniques, as a capillary matrix in a lateral flow type of diagnostic assay. This research was directed toward low-cost, disposable, and portable paper-based diagnos...
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| Veröffentlicht in: | Analytical and bioanalytical chemistry 2014-09, Vol.406 (24), p.5955-5965 |
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| creator | Teerinen, Tuija Lappalainen, Timo Erho, Tomi |
| description | Morphine was used as a model analyte to examine the possibility of using cellulose, physically modified by papermaking and converting techniques, as a capillary matrix in a lateral flow type of diagnostic assay. This research was directed toward low-cost, disposable, and portable paper-based diagnostics, with the aim of addressing the analytical performance of paper as a substrate in the analysis for drugs of abuse. Antibody Fab fragments were used as sensing molecules, and gold nanoparticle detection was employed. Inkjet printing was used to pattern sensing biomolecules as detection zones on paper. To validate the usefulness of paper as a diagnostic platform, the principle of a direct sandwich assay, based on immunocomplex formation between morphine and the anti-morphine Fab fragment and detection of the formed immunocomplex by another Fab fragment, was implemented. Results were compared with that achieved by using nitrocellulose as a reference material. Possible interfering from the sample matrix on assay quality was investigated with spiked oral fluid samples. Under optimized conditions, a visually assessed limit of detection for the sandwich assay was 1 ng/mL, indicating that the paper-based test devices developed in this work can perform screening for drugs of abuse and can fulfill the requirement for a sensitive assay in diagnostically relevant ranges.
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| doi_str_mv | 10.1007/s00216-014-8001-7 |
| format | Article |
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Fig
ᅟ</description><identifier>ISSN: 1618-2642</identifier><identifier>EISSN: 1618-2650</identifier><identifier>DOI: 10.1007/s00216-014-8001-7</identifier><identifier>PMID: 25023970</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Analysis ; Analytical Chemistry ; Assaying ; Biochemistry ; Biological assay ; Blood vessels ; Cellulose ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Collodion - chemistry ; Contact angle ; Detection ; Diagnostic systems ; Drugs ; Food Science ; Fragments ; Humans ; Hydrogen bonds ; Immunoassay - instrumentation ; Immunoassay - methods ; Infectious diseases ; Laboratory Medicine ; Mathematical analysis ; Monitoring/Environmental Analysis ; Morphine ; Morphine - analysis ; Point of care testing ; Porous materials ; Reagents ; Research Paper ; Saliva - chemistry ; Substance Abuse Detection - instrumentation ; Substance Abuse Detection - methods</subject><ispartof>Analytical and bioanalytical chemistry, 2014-09, Vol.406 (24), p.5955-5965</ispartof><rights>Springer-Verlag Berlin Heidelberg 2014</rights><rights>COPYRIGHT 2014 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c608t-3dd00ca40cf8f57cefd0555fba5bfc993ed3327e7d6aa6af09a78d43d851c27e3</citedby><cites>FETCH-LOGICAL-c608t-3dd00ca40cf8f57cefd0555fba5bfc993ed3327e7d6aa6af09a78d43d851c27e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00216-014-8001-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00216-014-8001-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25023970$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Teerinen, Tuija</creatorcontrib><creatorcontrib>Lappalainen, Timo</creatorcontrib><creatorcontrib>Erho, Tomi</creatorcontrib><title>A paper-based lateral flow assay for morphine</title><title>Analytical and bioanalytical chemistry</title><addtitle>Anal Bioanal Chem</addtitle><addtitle>Anal Bioanal Chem</addtitle><description>Morphine was used as a model analyte to examine the possibility of using cellulose, physically modified by papermaking and converting techniques, as a capillary matrix in a lateral flow type of diagnostic assay. This research was directed toward low-cost, disposable, and portable paper-based diagnostics, with the aim of addressing the analytical performance of paper as a substrate in the analysis for drugs of abuse. Antibody Fab fragments were used as sensing molecules, and gold nanoparticle detection was employed. Inkjet printing was used to pattern sensing biomolecules as detection zones on paper. To validate the usefulness of paper as a diagnostic platform, the principle of a direct sandwich assay, based on immunocomplex formation between morphine and the anti-morphine Fab fragment and detection of the formed immunocomplex by another Fab fragment, was implemented. Results were compared with that achieved by using nitrocellulose as a reference material. Possible interfering from the sample matrix on assay quality was investigated with spiked oral fluid samples. Under optimized conditions, a visually assessed limit of detection for the sandwich assay was 1 ng/mL, indicating that the paper-based test devices developed in this work can perform screening for drugs of abuse and can fulfill the requirement for a sensitive assay in diagnostically relevant ranges.
Fig
ᅟ</description><subject>Analysis</subject><subject>Analytical Chemistry</subject><subject>Assaying</subject><subject>Biochemistry</subject><subject>Biological assay</subject><subject>Blood vessels</subject><subject>Cellulose</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Collodion - chemistry</subject><subject>Contact angle</subject><subject>Detection</subject><subject>Diagnostic systems</subject><subject>Drugs</subject><subject>Food Science</subject><subject>Fragments</subject><subject>Humans</subject><subject>Hydrogen bonds</subject><subject>Immunoassay - instrumentation</subject><subject>Immunoassay - methods</subject><subject>Infectious diseases</subject><subject>Laboratory Medicine</subject><subject>Mathematical analysis</subject><subject>Monitoring/Environmental Analysis</subject><subject>Morphine</subject><subject>Morphine - analysis</subject><subject>Point of care testing</subject><subject>Porous materials</subject><subject>Reagents</subject><subject>Research Paper</subject><subject>Saliva - 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Academic</collection><jtitle>Analytical and bioanalytical chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Teerinen, Tuija</au><au>Lappalainen, Timo</au><au>Erho, Tomi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A paper-based lateral flow assay for morphine</atitle><jtitle>Analytical and bioanalytical chemistry</jtitle><stitle>Anal Bioanal Chem</stitle><addtitle>Anal Bioanal Chem</addtitle><date>2014-09-01</date><risdate>2014</risdate><volume>406</volume><issue>24</issue><spage>5955</spage><epage>5965</epage><pages>5955-5965</pages><issn>1618-2642</issn><eissn>1618-2650</eissn><abstract>Morphine was used as a model analyte to examine the possibility of using cellulose, physically modified by papermaking and converting techniques, as a capillary matrix in a lateral flow type of diagnostic assay. This research was directed toward low-cost, disposable, and portable paper-based diagnostics, with the aim of addressing the analytical performance of paper as a substrate in the analysis for drugs of abuse. Antibody Fab fragments were used as sensing molecules, and gold nanoparticle detection was employed. Inkjet printing was used to pattern sensing biomolecules as detection zones on paper. To validate the usefulness of paper as a diagnostic platform, the principle of a direct sandwich assay, based on immunocomplex formation between morphine and the anti-morphine Fab fragment and detection of the formed immunocomplex by another Fab fragment, was implemented. Results were compared with that achieved by using nitrocellulose as a reference material. Possible interfering from the sample matrix on assay quality was investigated with spiked oral fluid samples. Under optimized conditions, a visually assessed limit of detection for the sandwich assay was 1 ng/mL, indicating that the paper-based test devices developed in this work can perform screening for drugs of abuse and can fulfill the requirement for a sensitive assay in diagnostically relevant ranges.
Fig
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| subjects | Analysis Analytical Chemistry Assaying Biochemistry Biological assay Blood vessels Cellulose Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Collodion - chemistry Contact angle Detection Diagnostic systems Drugs Food Science Fragments Humans Hydrogen bonds Immunoassay - instrumentation Immunoassay - methods Infectious diseases Laboratory Medicine Mathematical analysis Monitoring/Environmental Analysis Morphine Morphine - analysis Point of care testing Porous materials Reagents Research Paper Saliva - chemistry Substance Abuse Detection - instrumentation Substance Abuse Detection - methods |
| title | A paper-based lateral flow assay for morphine |
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