Growing Self-Organizing Mini-Guts from a Single Intestinal Stem Cell: Mechanism and Applications
Recent examples have highlighted how stem cells have the capability to initiate morphogenesis in vitro; that is, to generate complex structures in culture that closely parallel their in vivo counterparts. Lgr5, the receptor for the Wnt-agonistic R-spondins, marks stem cells in multiple adult organs...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 2013-06, Vol.340 (6137), p.1190-1194 |
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| creator | Sato, Toshiro Clevers, Hans |
| description | Recent examples have highlighted how stem cells have the capability to initiate morphogenesis in vitro; that is, to generate complex structures in culture that closely parallel their in vivo counterparts. Lgr5, the receptor for the Wnt-agonistic R-spondins, marks stem cells in multiple adult organs of mice and humans. In R-spondin-based three-dimensional cultures, these Lgr5 stem cells can grow into ever-expanding epithelial organoids that retain their original organ identity. Single Lgr5 stem cells derived from the intestine can be cultured to build epithelial structures that retain hallmarks of the in vivo epithelium. Here, we review the mechanisms that support this notable example of self-organization and discuss applications of this technology for stem cell research, disease modeling (e.g., for colorectal cancer and cystic fibrosis), and regenerative medicine. |
| doi_str_mv | 10.1126/science.1234852 |
| format | Article |
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| ispartof | Science (American Association for the Advancement of Science), 2013-06, Vol.340 (6137), p.1190-1194 |
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| subjects | Adult Stem Cells - physiology Animals Cell Count Cell culture Cell Culture Techniques Cell lines Cellular differentiation Construction Crypts Culture Cystic fibrosis Daughter cells Disease Models, Animal Enteroendocrine cells Ephrin-B1 - metabolism Epithelial cells Epithelium Humans Intestinal Mucosa - physiology Intestine, Small - growth & development Intestines Mesenchymal stem cells Mice Morphogenesis Organoids Organs Receptors, G-Protein-Coupled - genetics Regenerative Medicine REVIEWS Stem Cell Niche Stem cells Three dimensional Wnt Proteins - metabolism |
| title | Growing Self-Organizing Mini-Guts from a Single Intestinal Stem Cell: Mechanism and Applications |
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