Bone morphogenetic proteins (BMP6 and BMP7) enhance the protective effect of neurotrophins on cultured septal cholinergic neurons during hypoglycemia
The effects of two bone morphogenetic proteins (BMP6, BMP7), alone and in combination with neurotrophins, were tested on cultures of embryonic day 15 rat septum. A week‐long exposure to BMP6 or BMP7 in the optimal concentration range of 2–5 n m increased the activity of choline acetyltransferase (Ch...
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| Veröffentlicht in: | Journal of neurochemistry 2001-04, Vol.77 (2), p.691-699 |
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| creator | Nonner, Doris Barrett, Ellen F. Kaplan, Paul Barrett, John N. |
| description | The effects of two bone morphogenetic proteins (BMP6, BMP7), alone and in combination with neurotrophins, were tested on cultures of embryonic day 15 rat septum. A week‐long exposure to BMP6 or BMP7 in the optimal concentration range of 2–5 n m increased the activity of choline acetyltransferase (ChAT) by 1.6–2‐fold, in both septal and combined septal−hippocampal cultures. The increase in ChAT activity reached significance after 4 days and continued to increase over an 11‐day exposure. Under control culture conditions neither BMP significantly altered the number of cholinergic neurons, and BMP effects on ChAT activity were less than linearly additive with those of nerve growth factor. The effects of BMPs and BMP + neurotrophin combinations were also assayed under two stress conditions: low‐density culture and hypoglycemia. In low‐density cultures BMPs and BMP + neurotrophin combinations preserved ChAT activity more effectively than neurotrophins alone. During 24 h hypoglycemic stress, BMPs alone did not preserve ChAT activity, but BMP + neurotrophin combinations preserved ChAT activity much more effectively than neurotrophins alone. These results demonstrate that BMP6 and BMP7 enhance ChAT activity under control and low‐density stress conditions, and that during a hypoglycemic stress their trophic effect requires and complements that exerted by neurotrophins. |
| doi_str_mv | 10.1046/j.1471-4159.2001.00273.x |
| format | Article |
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A week‐long exposure to BMP6 or BMP7 in the optimal concentration range of 2–5 n m increased the activity of choline acetyltransferase (ChAT) by 1.6–2‐fold, in both septal and combined septal−hippocampal cultures. The increase in ChAT activity reached significance after 4 days and continued to increase over an 11‐day exposure. Under control culture conditions neither BMP significantly altered the number of cholinergic neurons, and BMP effects on ChAT activity were less than linearly additive with those of nerve growth factor. The effects of BMPs and BMP + neurotrophin combinations were also assayed under two stress conditions: low‐density culture and hypoglycemia. In low‐density cultures BMPs and BMP + neurotrophin combinations preserved ChAT activity more effectively than neurotrophins alone. During 24 h hypoglycemic stress, BMPs alone did not preserve ChAT activity, but BMP + neurotrophin combinations preserved ChAT activity much more effectively than neurotrophins alone. These results demonstrate that BMP6 and BMP7 enhance ChAT activity under control and low‐density stress conditions, and that during a hypoglycemic stress their trophic effect requires and complements that exerted by neurotrophins.</description><identifier>ISSN: 0022-3042</identifier><identifier>EISSN: 1471-4159</identifier><identifier>DOI: 10.1046/j.1471-4159.2001.00273.x</identifier><identifier>PMID: 11299331</identifier><identifier>CODEN: JONRA9</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Animals ; Biochemistry and metabolism ; Biological and medical sciences ; Bone Morphogenetic Protein 6 ; Bone Morphogenetic Protein 7 ; bone morphogenetic proteins ; Bone Morphogenetic Proteins - pharmacology ; Brain-Derived Neurotrophic Factor - pharmacology ; Cells, Cultured ; Central nervous system ; choline acetyltransferase ; Choline O-Acetyltransferase - biosynthesis ; Drug Synergism ; Fundamental and applied biological sciences. Psychology ; Hippocampus - cytology ; Hippocampus - embryology ; hypoglycemia ; Hypoglycemia - metabolism ; Nerve Growth Factor - pharmacology ; Nerve Growth Factors - pharmacology ; Nerve Tissue Proteins - biosynthesis ; Neurons - drug effects ; Neurons - metabolism ; Neuroprotective Agents - pharmacology ; Neurotrophin 3 - pharmacology ; neurotrophins ; Rats ; Rats, Sprague-Dawley ; septal cholinergic neurons ; Septum Pellucidum - cytology ; Septum Pellucidum - embryology ; stress protection ; Stress, Physiological - metabolism ; Transforming Growth Factor beta ; Vertebrates: nervous system and sense organs</subject><ispartof>Journal of neurochemistry, 2001-04, Vol.77 (2), p.691-699</ispartof><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4913-e711f4395314e168235e2035ea49ae6169646be115f6aa43163af01e0878305d3</citedby><cites>FETCH-LOGICAL-c4913-e711f4395314e168235e2035ea49ae6169646be115f6aa43163af01e0878305d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1471-4159.2001.00273.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1471-4159.2001.00273.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1074819$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11299331$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nonner, Doris</creatorcontrib><creatorcontrib>Barrett, Ellen F.</creatorcontrib><creatorcontrib>Kaplan, Paul</creatorcontrib><creatorcontrib>Barrett, John N.</creatorcontrib><title>Bone morphogenetic proteins (BMP6 and BMP7) enhance the protective effect of neurotrophins on cultured septal cholinergic neurons during hypoglycemia</title><title>Journal of neurochemistry</title><addtitle>J Neurochem</addtitle><description>The effects of two bone morphogenetic proteins (BMP6, BMP7), alone and in combination with neurotrophins, were tested on cultures of embryonic day 15 rat septum. A week‐long exposure to BMP6 or BMP7 in the optimal concentration range of 2–5 n m increased the activity of choline acetyltransferase (ChAT) by 1.6–2‐fold, in both septal and combined septal−hippocampal cultures. The increase in ChAT activity reached significance after 4 days and continued to increase over an 11‐day exposure. Under control culture conditions neither BMP significantly altered the number of cholinergic neurons, and BMP effects on ChAT activity were less than linearly additive with those of nerve growth factor. The effects of BMPs and BMP + neurotrophin combinations were also assayed under two stress conditions: low‐density culture and hypoglycemia. In low‐density cultures BMPs and BMP + neurotrophin combinations preserved ChAT activity more effectively than neurotrophins alone. During 24 h hypoglycemic stress, BMPs alone did not preserve ChAT activity, but BMP + neurotrophin combinations preserved ChAT activity much more effectively than neurotrophins alone. These results demonstrate that BMP6 and BMP7 enhance ChAT activity under control and low‐density stress conditions, and that during a hypoglycemic stress their trophic effect requires and complements that exerted by neurotrophins.</description><subject>Animals</subject><subject>Biochemistry and metabolism</subject><subject>Biological and medical sciences</subject><subject>Bone Morphogenetic Protein 6</subject><subject>Bone Morphogenetic Protein 7</subject><subject>bone morphogenetic proteins</subject><subject>Bone Morphogenetic Proteins - pharmacology</subject><subject>Brain-Derived Neurotrophic Factor - pharmacology</subject><subject>Cells, Cultured</subject><subject>Central nervous system</subject><subject>choline acetyltransferase</subject><subject>Choline O-Acetyltransferase - biosynthesis</subject><subject>Drug Synergism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hippocampus - cytology</subject><subject>Hippocampus - embryology</subject><subject>hypoglycemia</subject><subject>Hypoglycemia - metabolism</subject><subject>Nerve Growth Factor - pharmacology</subject><subject>Nerve Growth Factors - pharmacology</subject><subject>Nerve Tissue Proteins - biosynthesis</subject><subject>Neurons - drug effects</subject><subject>Neurons - metabolism</subject><subject>Neuroprotective Agents - pharmacology</subject><subject>Neurotrophin 3 - pharmacology</subject><subject>neurotrophins</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>septal cholinergic neurons</subject><subject>Septum Pellucidum - cytology</subject><subject>Septum Pellucidum - embryology</subject><subject>stress protection</subject><subject>Stress, Physiological - metabolism</subject><subject>Transforming Growth Factor beta</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0022-3042</issn><issn>1471-4159</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkcuO1DAQRS0EYnoGfgF5gRCzSHDFjpMsWDAtnhoeC1hbHqfScSuxg53A9IfwvzjTLWDJxi6VT_mq7iWEAsuBCflin4OoIBNQNnnBGOSMFRXPb--RzZ-H-2STukXGmSjOyHmM-wRKIeEhOQMomoZz2JBfV94hHX2Yer9Dh7M1dAp-RusifX718Yuk2rU0FdUlRddrZ5DOPR4hM9sfSLHrUkV9Rx0uqR381K_j3lGzDPMSsKURp1kP1PR-sA7DLsncwQlrl2DdjvaHye-Gg8HR6kfkQaeHiI9P9wX59ub11-277Prz2_fbV9eZEQ3wDCuATvCm5CAQZF3wEguWDi0ajRJkk_a9QYCyk1oLDpLrjgGyuqo5K1t-QZ4d_03bfF8wzmq00eAwaId-iQpqVjPJZALrI2iCjzFgp6ZgRx0OCphaI1F7tTqvVufVGom6i0TdptEnJ43lZsT27-ApgwQ8PQE6Gj10IXls4z8ClaihSdjLI_bTDnj4b3314dN2rfhvwq6oAA</recordid><startdate>200104</startdate><enddate>200104</enddate><creator>Nonner, Doris</creator><creator>Barrett, Ellen F.</creator><creator>Kaplan, Paul</creator><creator>Barrett, John N.</creator><general>Blackwell Science Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope></search><sort><creationdate>200104</creationdate><title>Bone morphogenetic proteins (BMP6 and BMP7) enhance the protective effect of neurotrophins on cultured septal cholinergic neurons during hypoglycemia</title><author>Nonner, Doris ; Barrett, Ellen F. ; Kaplan, Paul ; Barrett, John N.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4913-e711f4395314e168235e2035ea49ae6169646be115f6aa43163af01e0878305d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Biochemistry and metabolism</topic><topic>Biological and medical sciences</topic><topic>Bone Morphogenetic Protein 6</topic><topic>Bone Morphogenetic Protein 7</topic><topic>bone morphogenetic proteins</topic><topic>Bone Morphogenetic Proteins - pharmacology</topic><topic>Brain-Derived Neurotrophic Factor - pharmacology</topic><topic>Cells, Cultured</topic><topic>Central nervous system</topic><topic>choline acetyltransferase</topic><topic>Choline O-Acetyltransferase - biosynthesis</topic><topic>Drug Synergism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hippocampus - cytology</topic><topic>Hippocampus - embryology</topic><topic>hypoglycemia</topic><topic>Hypoglycemia - metabolism</topic><topic>Nerve Growth Factor - pharmacology</topic><topic>Nerve Growth Factors - pharmacology</topic><topic>Nerve Tissue Proteins - biosynthesis</topic><topic>Neurons - drug effects</topic><topic>Neurons - metabolism</topic><topic>Neuroprotective Agents - pharmacology</topic><topic>Neurotrophin 3 - pharmacology</topic><topic>neurotrophins</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>septal cholinergic neurons</topic><topic>Septum Pellucidum - cytology</topic><topic>Septum Pellucidum - embryology</topic><topic>stress protection</topic><topic>Stress, Physiological - metabolism</topic><topic>Transforming Growth Factor beta</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nonner, Doris</creatorcontrib><creatorcontrib>Barrett, Ellen F.</creatorcontrib><creatorcontrib>Kaplan, Paul</creatorcontrib><creatorcontrib>Barrett, John N.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><jtitle>Journal of neurochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nonner, Doris</au><au>Barrett, Ellen F.</au><au>Kaplan, Paul</au><au>Barrett, John N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bone morphogenetic proteins (BMP6 and BMP7) enhance the protective effect of neurotrophins on cultured septal cholinergic neurons during hypoglycemia</atitle><jtitle>Journal of neurochemistry</jtitle><addtitle>J Neurochem</addtitle><date>2001-04</date><risdate>2001</risdate><volume>77</volume><issue>2</issue><spage>691</spage><epage>699</epage><pages>691-699</pages><issn>0022-3042</issn><eissn>1471-4159</eissn><coden>JONRA9</coden><abstract>The effects of two bone morphogenetic proteins (BMP6, BMP7), alone and in combination with neurotrophins, were tested on cultures of embryonic day 15 rat septum. A week‐long exposure to BMP6 or BMP7 in the optimal concentration range of 2–5 n m increased the activity of choline acetyltransferase (ChAT) by 1.6–2‐fold, in both septal and combined septal−hippocampal cultures. The increase in ChAT activity reached significance after 4 days and continued to increase over an 11‐day exposure. Under control culture conditions neither BMP significantly altered the number of cholinergic neurons, and BMP effects on ChAT activity were less than linearly additive with those of nerve growth factor. The effects of BMPs and BMP + neurotrophin combinations were also assayed under two stress conditions: low‐density culture and hypoglycemia. In low‐density cultures BMPs and BMP + neurotrophin combinations preserved ChAT activity more effectively than neurotrophins alone. During 24 h hypoglycemic stress, BMPs alone did not preserve ChAT activity, but BMP + neurotrophin combinations preserved ChAT activity much more effectively than neurotrophins alone. These results demonstrate that BMP6 and BMP7 enhance ChAT activity under control and low‐density stress conditions, and that during a hypoglycemic stress their trophic effect requires and complements that exerted by neurotrophins.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>11299331</pmid><doi>10.1046/j.1471-4159.2001.00273.x</doi><tpages>9</tpages></addata></record> |
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| ispartof | Journal of neurochemistry, 2001-04, Vol.77 (2), p.691-699 |
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| subjects | Animals Biochemistry and metabolism Biological and medical sciences Bone Morphogenetic Protein 6 Bone Morphogenetic Protein 7 bone morphogenetic proteins Bone Morphogenetic Proteins - pharmacology Brain-Derived Neurotrophic Factor - pharmacology Cells, Cultured Central nervous system choline acetyltransferase Choline O-Acetyltransferase - biosynthesis Drug Synergism Fundamental and applied biological sciences. Psychology Hippocampus - cytology Hippocampus - embryology hypoglycemia Hypoglycemia - metabolism Nerve Growth Factor - pharmacology Nerve Growth Factors - pharmacology Nerve Tissue Proteins - biosynthesis Neurons - drug effects Neurons - metabolism Neuroprotective Agents - pharmacology Neurotrophin 3 - pharmacology neurotrophins Rats Rats, Sprague-Dawley septal cholinergic neurons Septum Pellucidum - cytology Septum Pellucidum - embryology stress protection Stress, Physiological - metabolism Transforming Growth Factor beta Vertebrates: nervous system and sense organs |
| title | Bone morphogenetic proteins (BMP6 and BMP7) enhance the protective effect of neurotrophins on cultured septal cholinergic neurons during hypoglycemia |
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