Differential effects of two-pore channel protein 1 and 2 silencing in MDA-MB-468 breast cancer cells
Two-pore channel proteins, TPC1 and TPC2, are calcium permeable ion channels found localized to the membranes of endolysosomal calcium stores. There is increasing interest in the role of TPC-mediated intracellular signaling in various pathologies; however their role in breast cancer has not been ext...
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Veröffentlicht in: | Biochemical and biophysical research communications 2016-09, Vol.477 (4), p.731-736 |
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creator | Jahidin, Aisyah H. Stewart, Teneale A. Thompson, Erik W. Roberts-Thomson, Sarah J. Monteith, Gregory R. |
description | Two-pore channel proteins, TPC1 and TPC2, are calcium permeable ion channels found localized to the membranes of endolysosomal calcium stores. There is increasing interest in the role of TPC-mediated intracellular signaling in various pathologies; however their role in breast cancer has not been extensively evaluated. TPC1 and TPC2 mRNA was present in all non-tumorigenic and tumorigenic breast cell lines assessed. Silencing of TPC2 but not TPC1 attenuated epidermal growth factor-induced vimentin expression in MDA-MB-468 breast cancer cells. This effect was not due to a general inhibition of epithelial to mesenchymal transition (EMT) as TPC2 silencing had no effect on epidermal growth factor (EGF)-induced changes on E-cadherin expression. TPC1 and TPC2 were also shown to differentially regulate cyclopiazonic acid (CPA)-mediated changes in cytosolic free Ca2+. These findings indicate potential differential regulation of signaling processes by TPC1 and TPC2 in breast cancer cells.
•TPC1 and TPC2 are present in tumorigenic and non-tumorigenic breast cell lines.•TPC2, but not TPC1, regulates EGF-induced vimentin in MDA-MB-468 cells.•TPC silencing differentially alters CPA-mediated changes in Ca2+ signaling. |
doi_str_mv | 10.1016/j.bbrc.2016.06.127 |
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•TPC1 and TPC2 are present in tumorigenic and non-tumorigenic breast cell lines.•TPC2, but not TPC1, regulates EGF-induced vimentin in MDA-MB-468 cells.•TPC silencing differentially alters CPA-mediated changes in Ca2+ signaling.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2016.06.127</identifier><identifier>PMID: 27353380</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Breast cancer ; Breast Neoplasms - metabolism ; Calcium ; Calcium - metabolism ; Calcium Channels - genetics ; Calcium Channels - metabolism ; Calcium Signaling ; Cell Line, Tumor ; Epidermal growth factor ; Female ; Gene Expression Regulation, Neoplastic ; Gene Silencing ; Humans ; Ion Channel Gating ; Receptor, Epidermal Growth Factor - metabolism ; Two-pore channel ; Vimentin ; Vimentin - metabolism</subject><ispartof>Biochemical and biophysical research communications, 2016-09, Vol.477 (4), p.731-736</ispartof><rights>2016 Elsevier Inc.</rights><rights>Copyright © 2016 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c400t-f371cacbcb6c717bbf42b686a03c907d3990ddd7a554da00e103ee37c6a88b6e3</citedby><cites>FETCH-LOGICAL-c400t-f371cacbcb6c717bbf42b686a03c907d3990ddd7a554da00e103ee37c6a88b6e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bbrc.2016.06.127$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27353380$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jahidin, Aisyah H.</creatorcontrib><creatorcontrib>Stewart, Teneale A.</creatorcontrib><creatorcontrib>Thompson, Erik W.</creatorcontrib><creatorcontrib>Roberts-Thomson, Sarah J.</creatorcontrib><creatorcontrib>Monteith, Gregory R.</creatorcontrib><title>Differential effects of two-pore channel protein 1 and 2 silencing in MDA-MB-468 breast cancer cells</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Two-pore channel proteins, TPC1 and TPC2, are calcium permeable ion channels found localized to the membranes of endolysosomal calcium stores. There is increasing interest in the role of TPC-mediated intracellular signaling in various pathologies; however their role in breast cancer has not been extensively evaluated. TPC1 and TPC2 mRNA was present in all non-tumorigenic and tumorigenic breast cell lines assessed. Silencing of TPC2 but not TPC1 attenuated epidermal growth factor-induced vimentin expression in MDA-MB-468 breast cancer cells. This effect was not due to a general inhibition of epithelial to mesenchymal transition (EMT) as TPC2 silencing had no effect on epidermal growth factor (EGF)-induced changes on E-cadherin expression. TPC1 and TPC2 were also shown to differentially regulate cyclopiazonic acid (CPA)-mediated changes in cytosolic free Ca2+. These findings indicate potential differential regulation of signaling processes by TPC1 and TPC2 in breast cancer cells.
•TPC1 and TPC2 are present in tumorigenic and non-tumorigenic breast cell lines.•TPC2, but not TPC1, regulates EGF-induced vimentin in MDA-MB-468 cells.•TPC silencing differentially alters CPA-mediated changes in Ca2+ signaling.</description><subject>Breast cancer</subject><subject>Breast Neoplasms - metabolism</subject><subject>Calcium</subject><subject>Calcium - metabolism</subject><subject>Calcium Channels - genetics</subject><subject>Calcium Channels - metabolism</subject><subject>Calcium Signaling</subject><subject>Cell Line, Tumor</subject><subject>Epidermal growth factor</subject><subject>Female</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Gene Silencing</subject><subject>Humans</subject><subject>Ion Channel Gating</subject><subject>Receptor, Epidermal Growth Factor - metabolism</subject><subject>Two-pore channel</subject><subject>Vimentin</subject><subject>Vimentin - metabolism</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kDtPxDAQhC0EguPxByiQS5qEdZyzE4mGN0ggGpDoLD824FPOOewciH-PTweUVDtazYx2P0IOGZQMmDiZlcZEW1ZZlyBKVskNMmHQQlExqDfJBABEUbXsZYfspjQDYKwW7TbZqSSfct7AhLhL33UYMYxe9xSztmOiQ0fHz6FYDBGpfdMhYE8XcRjRB8qoDo5WNPkeg_Xhleblw-VZ8XBe1KKhJqJOI7U6WIzUYt-nfbLV6T7hwc_cI8_XV08Xt8X9483dxdl9YWuAsei4ZFZbY42wkkljuroyohEauG1BOt624JyTejqtnQZABhyRSyt00xiBfI8cr3vzre9LTKOa-7S6QAcclkmxBmQLwOs6W6u11cYhpYidWkQ_1_FLMVArumqmVnTViq4CoTLdHDr66V-aObq_yC_ObDhdGzB_-eExqmR9poTOxwxWucH_1_8NWOWKMQ</recordid><startdate>20160902</startdate><enddate>20160902</enddate><creator>Jahidin, Aisyah H.</creator><creator>Stewart, Teneale A.</creator><creator>Thompson, Erik W.</creator><creator>Roberts-Thomson, Sarah J.</creator><creator>Monteith, Gregory R.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20160902</creationdate><title>Differential effects of two-pore channel protein 1 and 2 silencing in MDA-MB-468 breast cancer cells</title><author>Jahidin, Aisyah H. ; Stewart, Teneale A. ; Thompson, Erik W. ; Roberts-Thomson, Sarah J. ; Monteith, Gregory R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c400t-f371cacbcb6c717bbf42b686a03c907d3990ddd7a554da00e103ee37c6a88b6e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Breast cancer</topic><topic>Breast Neoplasms - metabolism</topic><topic>Calcium</topic><topic>Calcium - metabolism</topic><topic>Calcium Channels - genetics</topic><topic>Calcium Channels - metabolism</topic><topic>Calcium Signaling</topic><topic>Cell Line, Tumor</topic><topic>Epidermal growth factor</topic><topic>Female</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Gene Silencing</topic><topic>Humans</topic><topic>Ion Channel Gating</topic><topic>Receptor, Epidermal Growth Factor - metabolism</topic><topic>Two-pore channel</topic><topic>Vimentin</topic><topic>Vimentin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jahidin, Aisyah H.</creatorcontrib><creatorcontrib>Stewart, Teneale A.</creatorcontrib><creatorcontrib>Thompson, Erik W.</creatorcontrib><creatorcontrib>Roberts-Thomson, Sarah J.</creatorcontrib><creatorcontrib>Monteith, Gregory R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jahidin, Aisyah H.</au><au>Stewart, Teneale A.</au><au>Thompson, Erik W.</au><au>Roberts-Thomson, Sarah J.</au><au>Monteith, Gregory R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential effects of two-pore channel protein 1 and 2 silencing in MDA-MB-468 breast cancer cells</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2016-09-02</date><risdate>2016</risdate><volume>477</volume><issue>4</issue><spage>731</spage><epage>736</epage><pages>731-736</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Two-pore channel proteins, TPC1 and TPC2, are calcium permeable ion channels found localized to the membranes of endolysosomal calcium stores. There is increasing interest in the role of TPC-mediated intracellular signaling in various pathologies; however their role in breast cancer has not been extensively evaluated. TPC1 and TPC2 mRNA was present in all non-tumorigenic and tumorigenic breast cell lines assessed. Silencing of TPC2 but not TPC1 attenuated epidermal growth factor-induced vimentin expression in MDA-MB-468 breast cancer cells. This effect was not due to a general inhibition of epithelial to mesenchymal transition (EMT) as TPC2 silencing had no effect on epidermal growth factor (EGF)-induced changes on E-cadherin expression. TPC1 and TPC2 were also shown to differentially regulate cyclopiazonic acid (CPA)-mediated changes in cytosolic free Ca2+. These findings indicate potential differential regulation of signaling processes by TPC1 and TPC2 in breast cancer cells.
•TPC1 and TPC2 are present in tumorigenic and non-tumorigenic breast cell lines.•TPC2, but not TPC1, regulates EGF-induced vimentin in MDA-MB-468 cells.•TPC silencing differentially alters CPA-mediated changes in Ca2+ signaling.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>27353380</pmid><doi>10.1016/j.bbrc.2016.06.127</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Breast cancer Breast Neoplasms - metabolism Calcium Calcium - metabolism Calcium Channels - genetics Calcium Channels - metabolism Calcium Signaling Cell Line, Tumor Epidermal growth factor Female Gene Expression Regulation, Neoplastic Gene Silencing Humans Ion Channel Gating Receptor, Epidermal Growth Factor - metabolism Two-pore channel Vimentin Vimentin - metabolism |
title | Differential effects of two-pore channel protein 1 and 2 silencing in MDA-MB-468 breast cancer cells |
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