The role of regulatory B cells (Bregs) in the Tregs-amplifying effect of Sirolimus
Sirolimus can significantly amplify regulatory T cells (Tregs) in vivo and in vitro, but the specific mechanism of this has not been well documented. The role of regulatory B cells (Bregs) in the Tregs-amplifying effect of Sirolimus was investigated in peripheral blood mononuclear cells (PBMCs) in v...
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| Veröffentlicht in: | International immunopharmacology 2016-09, Vol.38, p.90-96 |
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| creator | Song, Jiyong Xiao, Li Du, Guosheng Gao, Yu Chen, Wen Yang, Shaozhen Fan, Wenmei Shi, Bingyi |
| description | Sirolimus can significantly amplify regulatory T cells (Tregs) in vivo and in vitro, but the specific mechanism of this has not been well documented. The role of regulatory B cells (Bregs) in the Tregs-amplifying effect of Sirolimus was investigated in peripheral blood mononuclear cells (PBMCs) in vitro in this study. The results showed that the percentages of both CD19+CD24+CD38+TGF-β1+ Bregs and CD19+CD24+CD38+IL-10+ Bregs to B cells were elevated by Sirolimus in PBMCs including B cells. Sirolimus significantly enhances the cytokine production of transforming growth factor-β1 (TGF-β1) and interleukin-10 (IL-10) in PBMCs with B cells, and the enhancement significantly decreased in PBMCs without B cells. The percentage of CD4+CD25+Foxp3+ Tregs to T cells was also elevated by Sirolimus in PBMCs including B cells. The elevation of Tregs percentage decreased in PBMCs without B cells and recovered when additional TGF-β1 and IL-10 were added. The amplification of Tregs by Sirolimus was partially inhibited when either TGF-β1 or IL-10 was neutralized, and it even disappeared when these two cytokines were both neutralized. These results indicate that Sirolimus can amplify Bregs and Tregs in PBMCs in vitro, and Bregs may be the why Sirolimus amplifies Tregs.
•It is the first study on Sirolimus amplifying Breg in vitro.•The role of B cells in Sirolimus amplifying Treg was intensively investigated.•A hypothesis that Breg is one link of Sirolimus amplifying Treg was suggested. |
| doi_str_mv | 10.1016/j.intimp.2016.05.014 |
| format | Article |
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•It is the first study on Sirolimus amplifying Breg in vitro.•The role of B cells in Sirolimus amplifying Treg was intensively investigated.•A hypothesis that Breg is one link of Sirolimus amplifying Treg was suggested.</description><identifier>ISSN: 1567-5769</identifier><identifier>EISSN: 1878-1705</identifier><identifier>DOI: 10.1016/j.intimp.2016.05.014</identifier><identifier>PMID: 27258186</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>B-Lymphocytes, Regulatory - drug effects ; B-Lymphocytes, Regulatory - immunology ; Breg ; Cell Communication ; Cells, Cultured ; Forkhead Transcription Factors - metabolism ; Humans ; Immunosuppressive agent ; Immunosuppressive Agents - pharmacology ; Interleukin-10 - metabolism ; Leukocytes, Mononuclear - drug effects ; Leukocytes, Mononuclear - immunology ; Lymphocyte Activation - drug effects ; Lymphocyte subset ; Organ transplantation ; Sirolimus ; Sirolimus - pharmacology ; T-Lymphocytes, Regulatory - drug effects ; T-Lymphocytes, Regulatory - immunology ; Transforming Growth Factor beta1 - metabolism ; Treg</subject><ispartof>International immunopharmacology, 2016-09, Vol.38, p.90-96</ispartof><rights>2016</rights><rights>Copyright © 2016. Published by Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c362t-b87602d2ff32cf3799ea1004c8a90ab43ea52362a87fde9df97ebeb463c15e353</citedby><cites>FETCH-LOGICAL-c362t-b87602d2ff32cf3799ea1004c8a90ab43ea52362a87fde9df97ebeb463c15e353</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.intimp.2016.05.014$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27258186$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Song, Jiyong</creatorcontrib><creatorcontrib>Xiao, Li</creatorcontrib><creatorcontrib>Du, Guosheng</creatorcontrib><creatorcontrib>Gao, Yu</creatorcontrib><creatorcontrib>Chen, Wen</creatorcontrib><creatorcontrib>Yang, Shaozhen</creatorcontrib><creatorcontrib>Fan, Wenmei</creatorcontrib><creatorcontrib>Shi, Bingyi</creatorcontrib><title>The role of regulatory B cells (Bregs) in the Tregs-amplifying effect of Sirolimus</title><title>International immunopharmacology</title><addtitle>Int Immunopharmacol</addtitle><description>Sirolimus can significantly amplify regulatory T cells (Tregs) in vivo and in vitro, but the specific mechanism of this has not been well documented. The role of regulatory B cells (Bregs) in the Tregs-amplifying effect of Sirolimus was investigated in peripheral blood mononuclear cells (PBMCs) in vitro in this study. The results showed that the percentages of both CD19+CD24+CD38+TGF-β1+ Bregs and CD19+CD24+CD38+IL-10+ Bregs to B cells were elevated by Sirolimus in PBMCs including B cells. Sirolimus significantly enhances the cytokine production of transforming growth factor-β1 (TGF-β1) and interleukin-10 (IL-10) in PBMCs with B cells, and the enhancement significantly decreased in PBMCs without B cells. The percentage of CD4+CD25+Foxp3+ Tregs to T cells was also elevated by Sirolimus in PBMCs including B cells. The elevation of Tregs percentage decreased in PBMCs without B cells and recovered when additional TGF-β1 and IL-10 were added. The amplification of Tregs by Sirolimus was partially inhibited when either TGF-β1 or IL-10 was neutralized, and it even disappeared when these two cytokines were both neutralized. These results indicate that Sirolimus can amplify Bregs and Tregs in PBMCs in vitro, and Bregs may be the why Sirolimus amplifies Tregs.
•It is the first study on Sirolimus amplifying Breg in vitro.•The role of B cells in Sirolimus amplifying Treg was intensively investigated.•A hypothesis that Breg is one link of Sirolimus amplifying Treg was suggested.</description><subject>B-Lymphocytes, Regulatory - drug effects</subject><subject>B-Lymphocytes, Regulatory - immunology</subject><subject>Breg</subject><subject>Cell Communication</subject><subject>Cells, Cultured</subject><subject>Forkhead Transcription Factors - metabolism</subject><subject>Humans</subject><subject>Immunosuppressive agent</subject><subject>Immunosuppressive Agents - pharmacology</subject><subject>Interleukin-10 - metabolism</subject><subject>Leukocytes, Mononuclear - drug effects</subject><subject>Leukocytes, Mononuclear - immunology</subject><subject>Lymphocyte Activation - drug effects</subject><subject>Lymphocyte subset</subject><subject>Organ transplantation</subject><subject>Sirolimus</subject><subject>Sirolimus - pharmacology</subject><subject>T-Lymphocytes, Regulatory - drug effects</subject><subject>T-Lymphocytes, Regulatory - immunology</subject><subject>Transforming Growth Factor beta1 - metabolism</subject><subject>Treg</subject><issn>1567-5769</issn><issn>1878-1705</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kF1LwzAUhoMobk7_gUgu9aI1aZsmvRHc8AsGgs7rkKYnM6MfM2mF_XtTNr30KjmH5z0neRC6pCSmhOa3m9i2vW22cRKqmLCY0OwITangIqKcsONwZzmPGM-LCTrzfkNI6Gf0FE0SnjBBRT5Fb6tPwK6rAXcGO1gPteo7t8NzrKGuPb6eh6a_wbbFfSBXYxWpZltbs7PtGoMxoPsx_G7DGNsM_hydGFV7uDicM_Tx-LBaPEfL16eXxf0y0mme9FEpeE6SKjEmTbRJeVGAooRkWqiCqDJLQbEkkEpwU0FRmYJDCWWWp5oySFk6Q9f7uVvXfQ3ge9lYP75atdANXlJBuCiyQAY026Padd47MHLrbKPcTlIiR5tyI_c25WhTEiaDzRC7OmwYygaqv9CvvgDc7QEI__y24KTXFloNlXVBi6w6-_-GH3Ywh4M</recordid><startdate>201609</startdate><enddate>201609</enddate><creator>Song, Jiyong</creator><creator>Xiao, Li</creator><creator>Du, Guosheng</creator><creator>Gao, Yu</creator><creator>Chen, Wen</creator><creator>Yang, Shaozhen</creator><creator>Fan, Wenmei</creator><creator>Shi, Bingyi</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201609</creationdate><title>The role of regulatory B cells (Bregs) in the Tregs-amplifying effect of Sirolimus</title><author>Song, Jiyong ; Xiao, Li ; Du, Guosheng ; Gao, Yu ; Chen, Wen ; Yang, Shaozhen ; Fan, Wenmei ; Shi, Bingyi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c362t-b87602d2ff32cf3799ea1004c8a90ab43ea52362a87fde9df97ebeb463c15e353</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>B-Lymphocytes, Regulatory - drug effects</topic><topic>B-Lymphocytes, Regulatory - immunology</topic><topic>Breg</topic><topic>Cell Communication</topic><topic>Cells, Cultured</topic><topic>Forkhead Transcription Factors - metabolism</topic><topic>Humans</topic><topic>Immunosuppressive agent</topic><topic>Immunosuppressive Agents - pharmacology</topic><topic>Interleukin-10 - metabolism</topic><topic>Leukocytes, Mononuclear - drug effects</topic><topic>Leukocytes, Mononuclear - immunology</topic><topic>Lymphocyte Activation - drug effects</topic><topic>Lymphocyte subset</topic><topic>Organ transplantation</topic><topic>Sirolimus</topic><topic>Sirolimus - pharmacology</topic><topic>T-Lymphocytes, Regulatory - drug effects</topic><topic>T-Lymphocytes, Regulatory - immunology</topic><topic>Transforming Growth Factor beta1 - metabolism</topic><topic>Treg</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Song, Jiyong</creatorcontrib><creatorcontrib>Xiao, Li</creatorcontrib><creatorcontrib>Du, Guosheng</creatorcontrib><creatorcontrib>Gao, Yu</creatorcontrib><creatorcontrib>Chen, Wen</creatorcontrib><creatorcontrib>Yang, Shaozhen</creatorcontrib><creatorcontrib>Fan, Wenmei</creatorcontrib><creatorcontrib>Shi, Bingyi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International immunopharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Song, Jiyong</au><au>Xiao, Li</au><au>Du, Guosheng</au><au>Gao, Yu</au><au>Chen, Wen</au><au>Yang, Shaozhen</au><au>Fan, Wenmei</au><au>Shi, Bingyi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The role of regulatory B cells (Bregs) in the Tregs-amplifying effect of Sirolimus</atitle><jtitle>International immunopharmacology</jtitle><addtitle>Int Immunopharmacol</addtitle><date>2016-09</date><risdate>2016</risdate><volume>38</volume><spage>90</spage><epage>96</epage><pages>90-96</pages><issn>1567-5769</issn><eissn>1878-1705</eissn><abstract>Sirolimus can significantly amplify regulatory T cells (Tregs) in vivo and in vitro, but the specific mechanism of this has not been well documented. The role of regulatory B cells (Bregs) in the Tregs-amplifying effect of Sirolimus was investigated in peripheral blood mononuclear cells (PBMCs) in vitro in this study. The results showed that the percentages of both CD19+CD24+CD38+TGF-β1+ Bregs and CD19+CD24+CD38+IL-10+ Bregs to B cells were elevated by Sirolimus in PBMCs including B cells. Sirolimus significantly enhances the cytokine production of transforming growth factor-β1 (TGF-β1) and interleukin-10 (IL-10) in PBMCs with B cells, and the enhancement significantly decreased in PBMCs without B cells. The percentage of CD4+CD25+Foxp3+ Tregs to T cells was also elevated by Sirolimus in PBMCs including B cells. The elevation of Tregs percentage decreased in PBMCs without B cells and recovered when additional TGF-β1 and IL-10 were added. The amplification of Tregs by Sirolimus was partially inhibited when either TGF-β1 or IL-10 was neutralized, and it even disappeared when these two cytokines were both neutralized. These results indicate that Sirolimus can amplify Bregs and Tregs in PBMCs in vitro, and Bregs may be the why Sirolimus amplifies Tregs.
•It is the first study on Sirolimus amplifying Breg in vitro.•The role of B cells in Sirolimus amplifying Treg was intensively investigated.•A hypothesis that Breg is one link of Sirolimus amplifying Treg was suggested.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>27258186</pmid><doi>10.1016/j.intimp.2016.05.014</doi><tpages>7</tpages></addata></record> |
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| subjects | B-Lymphocytes, Regulatory - drug effects B-Lymphocytes, Regulatory - immunology Breg Cell Communication Cells, Cultured Forkhead Transcription Factors - metabolism Humans Immunosuppressive agent Immunosuppressive Agents - pharmacology Interleukin-10 - metabolism Leukocytes, Mononuclear - drug effects Leukocytes, Mononuclear - immunology Lymphocyte Activation - drug effects Lymphocyte subset Organ transplantation Sirolimus Sirolimus - pharmacology T-Lymphocytes, Regulatory - drug effects T-Lymphocytes, Regulatory - immunology Transforming Growth Factor beta1 - metabolism Treg |
| title | The role of regulatory B cells (Bregs) in the Tregs-amplifying effect of Sirolimus |
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