Cytotoxic substances from Aspergillus fumigatus in oxygenated or poorly oxygenated environment
Aspergillus fumigatus often causes serious health problems. The airway of the human body, the most common initial site of damage, is always exposed to an oxygenated condition, and the oxygen concentration may play a critical role in the virulence of A. fumigatus. In this study, oxygen content, funga...
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| Veröffentlicht in: | Mycopathologia (1975) 2004-07, Vol.158 (1), p.1-7 |
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| container_title | Mycopathologia (1975) |
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| creator | Watanabe, Akira Kamei, Katsuhiko Sekine, Toshikazu Higurashi, Hiromi Ochiai, Eri Hashimoto, Yoshie Nishimura, Kazuko |
| description | Aspergillus fumigatus often causes serious health problems. The airway of the human body, the most common initial site of damage, is always exposed to an oxygenated condition, and the oxygen concentration may play a critical role in the virulence of A. fumigatus. In this study, oxygen content, fungal growth, the production of cytotoxic substance(s) in the fungal culture, and their relationship were investigated. Two clinical strains of A. fumigatus were cultured under certain oxygen contents (10, 14 and 20%), and cytotoxicity of their culture filtrates on murine macrophages and their fungal growth were evaluated. The components of these filtrates were analyzed by gas chromatography-mass spectrometry. All culture filtrates contained gliotoxin and showed potent cytotoxicity on macrophages at very low concentration. The amount of gliotoxin in the culture filtrate prepared at 10% oxygen was markedly less, but diminutions in fungal growth and cytotoxicity of this culture filtrate were negligible. These results suggest that a well-oxygenated condition is suitable for the production of gliotoxin by A. fumigatus. A significant role of cytotoxic substances(s) other than gliotoxin is also suggested. |
| doi_str_mv | 10.1023/b:myco.0000038439.56108.3c |
| format | Article |
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The airway of the human body, the most common initial site of damage, is always exposed to an oxygenated condition, and the oxygen concentration may play a critical role in the virulence of A. fumigatus. In this study, oxygen content, fungal growth, the production of cytotoxic substance(s) in the fungal culture, and their relationship were investigated. Two clinical strains of A. fumigatus were cultured under certain oxygen contents (10, 14 and 20%), and cytotoxicity of their culture filtrates on murine macrophages and their fungal growth were evaluated. The components of these filtrates were analyzed by gas chromatography-mass spectrometry. All culture filtrates contained gliotoxin and showed potent cytotoxicity on macrophages at very low concentration. The amount of gliotoxin in the culture filtrate prepared at 10% oxygen was markedly less, but diminutions in fungal growth and cytotoxicity of this culture filtrate were negligible. These results suggest that a well-oxygenated condition is suitable for the production of gliotoxin by A. fumigatus. A significant role of cytotoxic substances(s) other than gliotoxin is also suggested.</description><identifier>ISSN: 0301-486X</identifier><identifier>EISSN: 1573-0832</identifier><identifier>DOI: 10.1023/b:myco.0000038439.56108.3c</identifier><identifier>PMID: 15487313</identifier><language>eng</language><publisher>Netherlands: Springer Nature B.V</publisher><subject>Animals ; Aspergillosis - microbiology ; Aspergillus fumigatus ; Aspergillus fumigatus - growth & development ; Aspergillus fumigatus - isolation & purification ; Aspergillus fumigatus - metabolism ; Chromatography ; Cytotoxicity ; Fungi ; Gas Chromatography-Mass Spectrometry ; Gliotoxin - biosynthesis ; Gliotoxin - toxicity ; Humans ; Infections ; Macrophages, Peritoneal - drug effects ; Macrophages, Peritoneal - metabolism ; Male ; Mass spectrometry ; Mice ; Mice, Inbred BALB C ; Oxygen - metabolism ; Science ; Scientific imaging ; Virulence</subject><ispartof>Mycopathologia (1975), 2004-07, Vol.158 (1), p.1-7</ispartof><rights>Copyright (c) 2004 Kluwer Academic Publishers</rights><rights>Kluwer Academic Publishers 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c466t-69081e75cc4c2d470ff3a268c526cb35bf4bd71170d9e420dcbef1444ec823df3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15487313$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Watanabe, Akira</creatorcontrib><creatorcontrib>Kamei, Katsuhiko</creatorcontrib><creatorcontrib>Sekine, Toshikazu</creatorcontrib><creatorcontrib>Higurashi, Hiromi</creatorcontrib><creatorcontrib>Ochiai, Eri</creatorcontrib><creatorcontrib>Hashimoto, Yoshie</creatorcontrib><creatorcontrib>Nishimura, Kazuko</creatorcontrib><title>Cytotoxic substances from Aspergillus fumigatus in oxygenated or poorly oxygenated environment</title><title>Mycopathologia (1975)</title><addtitle>Mycopathologia</addtitle><description>Aspergillus fumigatus often causes serious health problems. The airway of the human body, the most common initial site of damage, is always exposed to an oxygenated condition, and the oxygen concentration may play a critical role in the virulence of A. fumigatus. In this study, oxygen content, fungal growth, the production of cytotoxic substance(s) in the fungal culture, and their relationship were investigated. Two clinical strains of A. fumigatus were cultured under certain oxygen contents (10, 14 and 20%), and cytotoxicity of their culture filtrates on murine macrophages and their fungal growth were evaluated. The components of these filtrates were analyzed by gas chromatography-mass spectrometry. All culture filtrates contained gliotoxin and showed potent cytotoxicity on macrophages at very low concentration. The amount of gliotoxin in the culture filtrate prepared at 10% oxygen was markedly less, but diminutions in fungal growth and cytotoxicity of this culture filtrate were negligible. These results suggest that a well-oxygenated condition is suitable for the production of gliotoxin by A. fumigatus. A significant role of cytotoxic substances(s) other than gliotoxin is also suggested.</description><subject>Animals</subject><subject>Aspergillosis - microbiology</subject><subject>Aspergillus fumigatus</subject><subject>Aspergillus fumigatus - growth & development</subject><subject>Aspergillus fumigatus - isolation & purification</subject><subject>Aspergillus fumigatus - metabolism</subject><subject>Chromatography</subject><subject>Cytotoxicity</subject><subject>Fungi</subject><subject>Gas Chromatography-Mass Spectrometry</subject><subject>Gliotoxin - biosynthesis</subject><subject>Gliotoxin - toxicity</subject><subject>Humans</subject><subject>Infections</subject><subject>Macrophages, Peritoneal - drug effects</subject><subject>Macrophages, Peritoneal - metabolism</subject><subject>Male</subject><subject>Mass spectrometry</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Oxygen - metabolism</subject><subject>Science</subject><subject>Scientific imaging</subject><subject>Virulence</subject><issn>0301-486X</issn><issn>1573-0832</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kUtLw0AQxxdRbK1-BQk9eEudfWR305sWX1DpRUEvLslmU1KSbN1NpPn2prZQ8eAwMMPwmwfzR2iMYYKB0Ot0WnXaTmBrVDIaTyKOQU6oPkJDHAkagqTkGA2BAg6Z5G8DdOb9CgD3Lk7RAEdMCorpEH3MusY2dlPowLepb5JaGx_kzlbBjV8btyzKsu0LbVUsk6bPijqwm25p6qQxWWBdsLbWld3voqm_CmfrytTNOTrJk9Kbi30codf7u5fZYzhfPDzNbuahZpw3IY9BYiMirZkmGROQ5zQhXOqIcJ3SKM1ZmgmMBWSxYQQynZocM8aMloRmOR2hq93ctbOfrfGNqgqvTVkmtbGtV1gCF7GIe3D8B1zZ1tX9bYoQTIBH_UP_hzADwqMemu4g7az3zuRq7YoqcZ3CoLY6qVv1_D5bqINO6kcnRXXffLnf0KaVyQ6te2HoN77EkVY</recordid><startdate>20040701</startdate><enddate>20040701</enddate><creator>Watanabe, Akira</creator><creator>Kamei, Katsuhiko</creator><creator>Sekine, Toshikazu</creator><creator>Higurashi, Hiromi</creator><creator>Ochiai, Eri</creator><creator>Hashimoto, Yoshie</creator><creator>Nishimura, Kazuko</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20040701</creationdate><title>Cytotoxic substances from Aspergillus fumigatus in oxygenated or poorly oxygenated environment</title><author>Watanabe, Akira ; Kamei, Katsuhiko ; Sekine, Toshikazu ; Higurashi, Hiromi ; Ochiai, Eri ; Hashimoto, Yoshie ; Nishimura, Kazuko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c466t-69081e75cc4c2d470ff3a268c526cb35bf4bd71170d9e420dcbef1444ec823df3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Aspergillosis - microbiology</topic><topic>Aspergillus fumigatus</topic><topic>Aspergillus fumigatus - growth & development</topic><topic>Aspergillus fumigatus - isolation & purification</topic><topic>Aspergillus fumigatus - metabolism</topic><topic>Chromatography</topic><topic>Cytotoxicity</topic><topic>Fungi</topic><topic>Gas Chromatography-Mass Spectrometry</topic><topic>Gliotoxin - biosynthesis</topic><topic>Gliotoxin - toxicity</topic><topic>Humans</topic><topic>Infections</topic><topic>Macrophages, Peritoneal - drug effects</topic><topic>Macrophages, Peritoneal - metabolism</topic><topic>Male</topic><topic>Mass spectrometry</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Oxygen - metabolism</topic><topic>Science</topic><topic>Scientific imaging</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Watanabe, Akira</creatorcontrib><creatorcontrib>Kamei, Katsuhiko</creatorcontrib><creatorcontrib>Sekine, Toshikazu</creatorcontrib><creatorcontrib>Higurashi, Hiromi</creatorcontrib><creatorcontrib>Ochiai, Eri</creatorcontrib><creatorcontrib>Hashimoto, Yoshie</creatorcontrib><creatorcontrib>Nishimura, Kazuko</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>ProQuest Biological Science Journals</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Mycopathologia (1975)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Watanabe, Akira</au><au>Kamei, Katsuhiko</au><au>Sekine, Toshikazu</au><au>Higurashi, Hiromi</au><au>Ochiai, Eri</au><au>Hashimoto, Yoshie</au><au>Nishimura, Kazuko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cytotoxic substances from Aspergillus fumigatus in oxygenated or poorly oxygenated environment</atitle><jtitle>Mycopathologia (1975)</jtitle><addtitle>Mycopathologia</addtitle><date>2004-07-01</date><risdate>2004</risdate><volume>158</volume><issue>1</issue><spage>1</spage><epage>7</epage><pages>1-7</pages><issn>0301-486X</issn><eissn>1573-0832</eissn><abstract>Aspergillus fumigatus often causes serious health problems. The airway of the human body, the most common initial site of damage, is always exposed to an oxygenated condition, and the oxygen concentration may play a critical role in the virulence of A. fumigatus. In this study, oxygen content, fungal growth, the production of cytotoxic substance(s) in the fungal culture, and their relationship were investigated. Two clinical strains of A. fumigatus were cultured under certain oxygen contents (10, 14 and 20%), and cytotoxicity of their culture filtrates on murine macrophages and their fungal growth were evaluated. The components of these filtrates were analyzed by gas chromatography-mass spectrometry. All culture filtrates contained gliotoxin and showed potent cytotoxicity on macrophages at very low concentration. The amount of gliotoxin in the culture filtrate prepared at 10% oxygen was markedly less, but diminutions in fungal growth and cytotoxicity of this culture filtrate were negligible. These results suggest that a well-oxygenated condition is suitable for the production of gliotoxin by A. fumigatus. A significant role of cytotoxic substances(s) other than gliotoxin is also suggested.</abstract><cop>Netherlands</cop><pub>Springer Nature B.V</pub><pmid>15487313</pmid><doi>10.1023/b:myco.0000038439.56108.3c</doi><tpages>7</tpages></addata></record> |
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| ispartof | Mycopathologia (1975), 2004-07, Vol.158 (1), p.1-7 |
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| subjects | Animals Aspergillosis - microbiology Aspergillus fumigatus Aspergillus fumigatus - growth & development Aspergillus fumigatus - isolation & purification Aspergillus fumigatus - metabolism Chromatography Cytotoxicity Fungi Gas Chromatography-Mass Spectrometry Gliotoxin - biosynthesis Gliotoxin - toxicity Humans Infections Macrophages, Peritoneal - drug effects Macrophages, Peritoneal - metabolism Male Mass spectrometry Mice Mice, Inbred BALB C Oxygen - metabolism Science Scientific imaging Virulence |
| title | Cytotoxic substances from Aspergillus fumigatus in oxygenated or poorly oxygenated environment |
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