Isolation of thermotolerant Vermamoeba vermiformis strains from water sources in Lanzarote Island, Canary Islands, Spain
In this study, twenty water samples were collected in the island of Lanzarote, Canary Islands, Spain in order to check for the presence of V. vermiformis strains in these samples. Water samples were cultured on 2% Non-Nutrient Agar (NNA) plates covered with a thin layer of heat killed E. coli and ch...
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| Veröffentlicht in: | Acta parasitologica 2016-09, Vol.61 (3), p.650-653 |
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| creator | Reyes-Batlle, María Wagner, Carolina Zamora-Herrera, Jonadab Vargas-Mesa, Alejandro Sifaoui, Ines González, Ana C. López-Arencibia, Atteneri Valladares, Basilio Martínez-Carretero, Enrique Piñero, José E. Lorenzo-Morales, Jacob |
| description | In this study, twenty water samples were collected in the island of Lanzarote, Canary Islands, Spain in order to check for the presence of
V. vermiformis
strains in these samples. Water samples were cultured on 2% Non-Nutrient Agar (NNA) plates covered with a thin layer of heat killed E. coli and checked daily for the presence of
Vermamoeba
. After a week,
V. vermiformis
amoebae were observed in 2 of the 20 processed samples (10%) incubated at room temperature and 37°C. Molecular characterization was carried out by amplifying the 18S rDNA gene and DNA sequencing in order to confirm the identity of the isolated amoebic strains. To the best of our knowledge, this is the first report on the presence of FLA in environmental sources in Lanzarote Island and the first report of
Vermamoeba vermiformis
in water sources in this island. Furthermore, the two strains isolated in this study were collected in recreational areas with close contact with humans and thus awareness should be raised. |
| doi_str_mv | 10.1515/ap-2016-0088 |
| format | Article |
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V. vermiformis
strains in these samples. Water samples were cultured on 2% Non-Nutrient Agar (NNA) plates covered with a thin layer of heat killed E. coli and checked daily for the presence of
Vermamoeba
. After a week,
V. vermiformis
amoebae were observed in 2 of the 20 processed samples (10%) incubated at room temperature and 37°C. Molecular characterization was carried out by amplifying the 18S rDNA gene and DNA sequencing in order to confirm the identity of the isolated amoebic strains. To the best of our knowledge, this is the first report on the presence of FLA in environmental sources in Lanzarote Island and the first report of
Vermamoeba vermiformis
in water sources in this island. Furthermore, the two strains isolated in this study were collected in recreational areas with close contact with humans and thus awareness should be raised.</description><identifier>ISSN: 1230-2821</identifier><identifier>EISSN: 1896-1851</identifier><identifier>DOI: 10.1515/ap-2016-0088</identifier><identifier>PMID: 27447234</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Amoeba - genetics ; Amoeba - isolation & purification ; Animal Systematics/Taxonomy/Biogeography ; Biomedical and Life Sciences ; Biomedicine ; Canary Islands ; DNA, Protozoan - genetics ; DNA, Ribosomal - genetics ; E coli ; Ecology ; Genotype ; Islands ; Lanzarote ; Medical Microbiology ; Microbiology ; Morphology ; Parasitology ; Pathogens ; Protozoa ; Research Note ; Spain ; water ; Water - parasitology</subject><ispartof>Acta parasitologica, 2016-09, Vol.61 (3), p.650-653</ispartof><rights>Witold Stefański Institute of Parasitology, Polish Academy of Sciences 2018</rights><rights>Copyright Walter de Gruyter GmbH 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c408t-13189e718a2e5e003484397b6b339ea74fe0c50273134921bcd6a3bd9e3875683</citedby><cites>FETCH-LOGICAL-c408t-13189e718a2e5e003484397b6b339ea74fe0c50273134921bcd6a3bd9e3875683</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1515/ap-2016-0088$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1515/ap-2016-0088$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27447234$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Reyes-Batlle, María</creatorcontrib><creatorcontrib>Wagner, Carolina</creatorcontrib><creatorcontrib>Zamora-Herrera, Jonadab</creatorcontrib><creatorcontrib>Vargas-Mesa, Alejandro</creatorcontrib><creatorcontrib>Sifaoui, Ines</creatorcontrib><creatorcontrib>González, Ana C.</creatorcontrib><creatorcontrib>López-Arencibia, Atteneri</creatorcontrib><creatorcontrib>Valladares, Basilio</creatorcontrib><creatorcontrib>Martínez-Carretero, Enrique</creatorcontrib><creatorcontrib>Piñero, José E.</creatorcontrib><creatorcontrib>Lorenzo-Morales, Jacob</creatorcontrib><title>Isolation of thermotolerant Vermamoeba vermiformis strains from water sources in Lanzarote Island, Canary Islands, Spain</title><title>Acta parasitologica</title><addtitle>Acta Parasit</addtitle><addtitle>Acta Parasitol</addtitle><description>In this study, twenty water samples were collected in the island of Lanzarote, Canary Islands, Spain in order to check for the presence of
V. vermiformis
strains in these samples. Water samples were cultured on 2% Non-Nutrient Agar (NNA) plates covered with a thin layer of heat killed E. coli and checked daily for the presence of
Vermamoeba
. After a week,
V. vermiformis
amoebae were observed in 2 of the 20 processed samples (10%) incubated at room temperature and 37°C. Molecular characterization was carried out by amplifying the 18S rDNA gene and DNA sequencing in order to confirm the identity of the isolated amoebic strains. To the best of our knowledge, this is the first report on the presence of FLA in environmental sources in Lanzarote Island and the first report of
Vermamoeba vermiformis
in water sources in this island. Furthermore, the two strains isolated in this study were collected in recreational areas with close contact with humans and thus awareness should be raised.</description><subject>Amoeba - genetics</subject><subject>Amoeba - isolation & purification</subject><subject>Animal Systematics/Taxonomy/Biogeography</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Canary Islands</subject><subject>DNA, Protozoan - genetics</subject><subject>DNA, Ribosomal - genetics</subject><subject>E coli</subject><subject>Ecology</subject><subject>Genotype</subject><subject>Islands</subject><subject>Lanzarote</subject><subject>Medical Microbiology</subject><subject>Microbiology</subject><subject>Morphology</subject><subject>Parasitology</subject><subject>Pathogens</subject><subject>Protozoa</subject><subject>Research Note</subject><subject>Spain</subject><subject>water</subject><subject>Water - parasitology</subject><issn>1230-2821</issn><issn>1896-1851</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkcur1TAQxoMo3ofuXEvAjYtTzatp6k4Oej1wwIWPbZm202svbVKT1Ov1r3cO5ygigptkBn7fZL58jD2R4oUsZfkSlkIJaQshnLvHzqWrbSFdKe9TrbQolFPyjF2kdCOEsc65h-xMVcZUSptz9n2XwgR5DJ6HgecvGOeQw4QRfOafqYM5YAv8G5XjEOhIPOUIo098iGHmt5Ax8hTW2GHio-d78D8ghox8lybw_YZvwUO8O7Vpwz8sJH_EHgwwJXx8ui_Zp7dvPm7fFfv3V7vt633RGeFyITUZwko6UFiiENo4o-uqta3WNUJlBhRdKVSlpTa1km3XW9BtX6N2VWmdvmTPj3OXGL6umHJDFjqcaBcMa2qkE9YaUTtL6LO_0Buy5Wm7A1VLqSolidocqS6GlCIOzRLHmQw2UjSHRBpYmkMizSERwp-ehq7tjP1v-FcEBLw6Arcw0Vf2eB3XOyr-ePwfc63UthQkLo7iRHP99f9U-ifyDaft</recordid><startdate>20160901</startdate><enddate>20160901</enddate><creator>Reyes-Batlle, María</creator><creator>Wagner, Carolina</creator><creator>Zamora-Herrera, Jonadab</creator><creator>Vargas-Mesa, Alejandro</creator><creator>Sifaoui, Ines</creator><creator>González, Ana C.</creator><creator>López-Arencibia, Atteneri</creator><creator>Valladares, Basilio</creator><creator>Martínez-Carretero, Enrique</creator><creator>Piñero, José E.</creator><creator>Lorenzo-Morales, Jacob</creator><general>Springer International Publishing</general><general>De Gruyter</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20160901</creationdate><title>Isolation of thermotolerant Vermamoeba vermiformis strains from water sources in Lanzarote Island, Canary Islands, Spain</title><author>Reyes-Batlle, María ; Wagner, Carolina ; Zamora-Herrera, Jonadab ; Vargas-Mesa, Alejandro ; Sifaoui, Ines ; González, Ana C. ; López-Arencibia, Atteneri ; Valladares, Basilio ; Martínez-Carretero, Enrique ; Piñero, José E. ; Lorenzo-Morales, Jacob</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-13189e718a2e5e003484397b6b339ea74fe0c50273134921bcd6a3bd9e3875683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Amoeba - 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Academic</collection><jtitle>Acta parasitologica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Reyes-Batlle, María</au><au>Wagner, Carolina</au><au>Zamora-Herrera, Jonadab</au><au>Vargas-Mesa, Alejandro</au><au>Sifaoui, Ines</au><au>González, Ana C.</au><au>López-Arencibia, Atteneri</au><au>Valladares, Basilio</au><au>Martínez-Carretero, Enrique</au><au>Piñero, José E.</au><au>Lorenzo-Morales, Jacob</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation of thermotolerant Vermamoeba vermiformis strains from water sources in Lanzarote Island, Canary Islands, Spain</atitle><jtitle>Acta parasitologica</jtitle><stitle>Acta Parasit</stitle><addtitle>Acta Parasitol</addtitle><date>2016-09-01</date><risdate>2016</risdate><volume>61</volume><issue>3</issue><spage>650</spage><epage>653</epage><pages>650-653</pages><issn>1230-2821</issn><eissn>1896-1851</eissn><abstract>In this study, twenty water samples were collected in the island of Lanzarote, Canary Islands, Spain in order to check for the presence of
V. vermiformis
strains in these samples. Water samples were cultured on 2% Non-Nutrient Agar (NNA) plates covered with a thin layer of heat killed E. coli and checked daily for the presence of
Vermamoeba
. After a week,
V. vermiformis
amoebae were observed in 2 of the 20 processed samples (10%) incubated at room temperature and 37°C. Molecular characterization was carried out by amplifying the 18S rDNA gene and DNA sequencing in order to confirm the identity of the isolated amoebic strains. To the best of our knowledge, this is the first report on the presence of FLA in environmental sources in Lanzarote Island and the first report of
Vermamoeba vermiformis
in water sources in this island. Furthermore, the two strains isolated in this study were collected in recreational areas with close contact with humans and thus awareness should be raised.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>27447234</pmid><doi>10.1515/ap-2016-0088</doi><tpages>4</tpages></addata></record> |
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| ispartof | Acta parasitologica, 2016-09, Vol.61 (3), p.650-653 |
| issn | 1230-2821 1896-1851 |
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| subjects | Amoeba - genetics Amoeba - isolation & purification Animal Systematics/Taxonomy/Biogeography Biomedical and Life Sciences Biomedicine Canary Islands DNA, Protozoan - genetics DNA, Ribosomal - genetics E coli Ecology Genotype Islands Lanzarote Medical Microbiology Microbiology Morphology Parasitology Pathogens Protozoa Research Note Spain water Water - parasitology |
| title | Isolation of thermotolerant Vermamoeba vermiformis strains from water sources in Lanzarote Island, Canary Islands, Spain |
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