An in vitro study of alkaline phosphatase sensitivity to mixture of aflatoxin B1 and fumonisin B1 in the hepatopancreas of coastal lagoon wild and farmed shrimp Litopenaeus vannamei
This study aimed to establish the combined effect of aflatoxin B 1 (AFB 1 ) and fumonisin B 1 (FB 1 ) on wild Litopenaeus vannamei hepatopancreas alkaline phosphatase (AP) activity compared with that of farmed shrimp. AP activity in hepatopancreas extract was confirmed by several specific inhibitor...
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| Veröffentlicht in: | Mycotoxin research 2016-08, Vol.32 (3), p.117-125 |
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| creator | Pérez-Acosta, Jesús A. Burgos-Hernandez, Armando Velázquez-Contreras, Carlos A. Márquez-Ríos, Enrique Torres-Arreola, Wilfrido Arvizu-Flores, Aldo A. Ezquerra-Brauer, J. Marina |
| description | This study aimed to establish the combined effect of aflatoxin B
1
(AFB
1
) and fumonisin B
1
(FB
1
) on wild
Litopenaeus vannamei
hepatopancreas alkaline phosphatase (AP) activity compared with that of farmed shrimp. AP activity in hepatopancreas extract was confirmed by several specific inhibitor assays. AP activity of wild shrimp was higher than that of farmed shrimp (
p
|
| doi_str_mv | 10.1007/s12550-016-0246-x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1805768606</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1805768606</sourcerecordid><originalsourceid>FETCH-LOGICAL-c259x-45cb24089d6f34172a6325c5bd60a89956475cab77577a3afddbe67630a672a93</originalsourceid><addsrcrecordid>eNp9kc2O1DAQhC0EYoeBB-CCfOQSsJP4J8dlxZ80Ehc4W53E2XhJ7OB2lsyD8X54yMKRiy3ZX5W6ugh5ydkbzph6i7wUghWMy4KVtSy2R-TAtVQFl1X5mBwYV7pQWusr8gzxjjFZ1VI_JVelYjXTXB_Ir2tPnaf3LsVAMa39mYaBwvQdJuctXcaAywgJ0FK0Hl1yGT3TFOjstrRG-wcfJkhhyz7vOAXf02Gdg3e4P-QzjZaOdsnQAr6LFvAi6wJggolOcBuCpz_d1O9qiLPtKY7RzQs9uayyHuyK9B68h9m65-TJABPaFw_3kXz78P7rzafi9OXj55vrU9GVotmKWnRtmYM2vRyqmqsS8l5EJ9peMtBNI2StRAetUkIpqGDo-9ZKJSsGMsNNdSSvd98lhh-rxWRmh52dJvA2rGi4ZkJJLfNij4TvaBcDYrSDWfL4EM-GM3Npy-xtmdyWubRltqx59WC_tjnxP8XfejJQ7gDmL39ro7kLa_Q58n9cfwOsyaPN</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1805768606</pqid></control><display><type>article</type><title>An in vitro study of alkaline phosphatase sensitivity to mixture of aflatoxin B1 and fumonisin B1 in the hepatopancreas of coastal lagoon wild and farmed shrimp Litopenaeus vannamei</title><source>MEDLINE</source><source>Springer Online Journals Complete</source><creator>Pérez-Acosta, Jesús A. ; Burgos-Hernandez, Armando ; Velázquez-Contreras, Carlos A. ; Márquez-Ríos, Enrique ; Torres-Arreola, Wilfrido ; Arvizu-Flores, Aldo A. ; Ezquerra-Brauer, J. Marina</creator><creatorcontrib>Pérez-Acosta, Jesús A. ; Burgos-Hernandez, Armando ; Velázquez-Contreras, Carlos A. ; Márquez-Ríos, Enrique ; Torres-Arreola, Wilfrido ; Arvizu-Flores, Aldo A. ; Ezquerra-Brauer, J. Marina</creatorcontrib><description>This study aimed to establish the combined effect of aflatoxin B
1
(AFB
1
) and fumonisin B
1
(FB
1
) on wild
Litopenaeus vannamei
hepatopancreas alkaline phosphatase (AP) activity compared with that of farmed shrimp. AP activity in hepatopancreas extract was confirmed by several specific inhibitor assays. AP activity of wild shrimp was higher than that of farmed shrimp (
p
< 0.05). However, AP activity from both wild and farmed shrimp was inhibited when incubated with AFB
1
and FB
1
. The greatest inhibition occurred when AP was incubated with a mixture of AFB
1
and FB
1
. The IC
50
for AFB
1
on AP activity of wild and farmed shrimp hepatopancreases was 0.790 and 0.398 μg/mL, respectively. The IC
50
of FB
1
was 0.87 μg/mL for wild shrimp and 0.69 μg/mL for farmed shrimp. These results suggest that, at the mycotoxins concentrations used in the study, AP from farmed
L. vannamei
was sensitive to the presence of both mycotoxins; however, AP is more sensitive to the combination of AFB
1
+ FB
1
suggesting a possible synergistic or potentiating inhibitory effect.</description><identifier>ISSN: 0178-7888</identifier><identifier>EISSN: 1867-1632</identifier><identifier>DOI: 10.1007/s12550-016-0246-x</identifier><identifier>PMID: 27040818</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Aflatoxin B1 - metabolism ; Alkaline Phosphatase - antagonists & inhibitors ; Animals ; Biomedical and Life Sciences ; Chemistry/Food Science ; Enzyme Inhibitors - metabolism ; Fumonisins - metabolism ; Hepatopancreas - enzymology ; Inhibitory Concentration 50 ; Life Sciences ; Medical Microbiology ; Medicine/Public Health ; Microbiology ; Original Article ; Penaeidae - enzymology</subject><ispartof>Mycotoxin research, 2016-08, Vol.32 (3), p.117-125</ispartof><rights>Society for Mycotoxin Research and Springer-Verlag Berlin Heidelberg 2016</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c259x-45cb24089d6f34172a6325c5bd60a89956475cab77577a3afddbe67630a672a93</citedby><cites>FETCH-LOGICAL-c259x-45cb24089d6f34172a6325c5bd60a89956475cab77577a3afddbe67630a672a93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12550-016-0246-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12550-016-0246-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27040818$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pérez-Acosta, Jesús A.</creatorcontrib><creatorcontrib>Burgos-Hernandez, Armando</creatorcontrib><creatorcontrib>Velázquez-Contreras, Carlos A.</creatorcontrib><creatorcontrib>Márquez-Ríos, Enrique</creatorcontrib><creatorcontrib>Torres-Arreola, Wilfrido</creatorcontrib><creatorcontrib>Arvizu-Flores, Aldo A.</creatorcontrib><creatorcontrib>Ezquerra-Brauer, J. Marina</creatorcontrib><title>An in vitro study of alkaline phosphatase sensitivity to mixture of aflatoxin B1 and fumonisin B1 in the hepatopancreas of coastal lagoon wild and farmed shrimp Litopenaeus vannamei</title><title>Mycotoxin research</title><addtitle>Mycotoxin Res</addtitle><addtitle>Mycotoxin Res</addtitle><description>This study aimed to establish the combined effect of aflatoxin B
1
(AFB
1
) and fumonisin B
1
(FB
1
) on wild
Litopenaeus vannamei
hepatopancreas alkaline phosphatase (AP) activity compared with that of farmed shrimp. AP activity in hepatopancreas extract was confirmed by several specific inhibitor assays. AP activity of wild shrimp was higher than that of farmed shrimp (
p
< 0.05). However, AP activity from both wild and farmed shrimp was inhibited when incubated with AFB
1
and FB
1
. The greatest inhibition occurred when AP was incubated with a mixture of AFB
1
and FB
1
. The IC
50
for AFB
1
on AP activity of wild and farmed shrimp hepatopancreases was 0.790 and 0.398 μg/mL, respectively. The IC
50
of FB
1
was 0.87 μg/mL for wild shrimp and 0.69 μg/mL for farmed shrimp. These results suggest that, at the mycotoxins concentrations used in the study, AP from farmed
L. vannamei
was sensitive to the presence of both mycotoxins; however, AP is more sensitive to the combination of AFB
1
+ FB
1
suggesting a possible synergistic or potentiating inhibitory effect.</description><subject>Aflatoxin B1 - metabolism</subject><subject>Alkaline Phosphatase - antagonists & inhibitors</subject><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Chemistry/Food Science</subject><subject>Enzyme Inhibitors - metabolism</subject><subject>Fumonisins - metabolism</subject><subject>Hepatopancreas - enzymology</subject><subject>Inhibitory Concentration 50</subject><subject>Life Sciences</subject><subject>Medical Microbiology</subject><subject>Medicine/Public Health</subject><subject>Microbiology</subject><subject>Original Article</subject><subject>Penaeidae - enzymology</subject><issn>0178-7888</issn><issn>1867-1632</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc2O1DAQhC0EYoeBB-CCfOQSsJP4J8dlxZ80Ehc4W53E2XhJ7OB2lsyD8X54yMKRiy3ZX5W6ugh5ydkbzph6i7wUghWMy4KVtSy2R-TAtVQFl1X5mBwYV7pQWusr8gzxjjFZ1VI_JVelYjXTXB_Ir2tPnaf3LsVAMa39mYaBwvQdJuctXcaAywgJ0FK0Hl1yGT3TFOjstrRG-wcfJkhhyz7vOAXf02Gdg3e4P-QzjZaOdsnQAr6LFvAi6wJggolOcBuCpz_d1O9qiLPtKY7RzQs9uayyHuyK9B68h9m65-TJABPaFw_3kXz78P7rzafi9OXj55vrU9GVotmKWnRtmYM2vRyqmqsS8l5EJ9peMtBNI2StRAetUkIpqGDo-9ZKJSsGMsNNdSSvd98lhh-rxWRmh52dJvA2rGi4ZkJJLfNij4TvaBcDYrSDWfL4EM-GM3Npy-xtmdyWubRltqx59WC_tjnxP8XfejJQ7gDmL39ro7kLa_Q58n9cfwOsyaPN</recordid><startdate>20160801</startdate><enddate>20160801</enddate><creator>Pérez-Acosta, Jesús A.</creator><creator>Burgos-Hernandez, Armando</creator><creator>Velázquez-Contreras, Carlos A.</creator><creator>Márquez-Ríos, Enrique</creator><creator>Torres-Arreola, Wilfrido</creator><creator>Arvizu-Flores, Aldo A.</creator><creator>Ezquerra-Brauer, J. Marina</creator><general>Springer Berlin Heidelberg</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20160801</creationdate><title>An in vitro study of alkaline phosphatase sensitivity to mixture of aflatoxin B1 and fumonisin B1 in the hepatopancreas of coastal lagoon wild and farmed shrimp Litopenaeus vannamei</title><author>Pérez-Acosta, Jesús A. ; Burgos-Hernandez, Armando ; Velázquez-Contreras, Carlos A. ; Márquez-Ríos, Enrique ; Torres-Arreola, Wilfrido ; Arvizu-Flores, Aldo A. ; Ezquerra-Brauer, J. Marina</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c259x-45cb24089d6f34172a6325c5bd60a89956475cab77577a3afddbe67630a672a93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Aflatoxin B1 - metabolism</topic><topic>Alkaline Phosphatase - antagonists & inhibitors</topic><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Chemistry/Food Science</topic><topic>Enzyme Inhibitors - metabolism</topic><topic>Fumonisins - metabolism</topic><topic>Hepatopancreas - enzymology</topic><topic>Inhibitory Concentration 50</topic><topic>Life Sciences</topic><topic>Medical Microbiology</topic><topic>Medicine/Public Health</topic><topic>Microbiology</topic><topic>Original Article</topic><topic>Penaeidae - enzymology</topic><toplevel>online_resources</toplevel><creatorcontrib>Pérez-Acosta, Jesús A.</creatorcontrib><creatorcontrib>Burgos-Hernandez, Armando</creatorcontrib><creatorcontrib>Velázquez-Contreras, Carlos A.</creatorcontrib><creatorcontrib>Márquez-Ríos, Enrique</creatorcontrib><creatorcontrib>Torres-Arreola, Wilfrido</creatorcontrib><creatorcontrib>Arvizu-Flores, Aldo A.</creatorcontrib><creatorcontrib>Ezquerra-Brauer, J. Marina</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Mycotoxin research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pérez-Acosta, Jesús A.</au><au>Burgos-Hernandez, Armando</au><au>Velázquez-Contreras, Carlos A.</au><au>Márquez-Ríos, Enrique</au><au>Torres-Arreola, Wilfrido</au><au>Arvizu-Flores, Aldo A.</au><au>Ezquerra-Brauer, J. Marina</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An in vitro study of alkaline phosphatase sensitivity to mixture of aflatoxin B1 and fumonisin B1 in the hepatopancreas of coastal lagoon wild and farmed shrimp Litopenaeus vannamei</atitle><jtitle>Mycotoxin research</jtitle><stitle>Mycotoxin Res</stitle><addtitle>Mycotoxin Res</addtitle><date>2016-08-01</date><risdate>2016</risdate><volume>32</volume><issue>3</issue><spage>117</spage><epage>125</epage><pages>117-125</pages><issn>0178-7888</issn><eissn>1867-1632</eissn><abstract>This study aimed to establish the combined effect of aflatoxin B
1
(AFB
1
) and fumonisin B
1
(FB
1
) on wild
Litopenaeus vannamei
hepatopancreas alkaline phosphatase (AP) activity compared with that of farmed shrimp. AP activity in hepatopancreas extract was confirmed by several specific inhibitor assays. AP activity of wild shrimp was higher than that of farmed shrimp (
p
< 0.05). However, AP activity from both wild and farmed shrimp was inhibited when incubated with AFB
1
and FB
1
. The greatest inhibition occurred when AP was incubated with a mixture of AFB
1
and FB
1
. The IC
50
for AFB
1
on AP activity of wild and farmed shrimp hepatopancreases was 0.790 and 0.398 μg/mL, respectively. The IC
50
of FB
1
was 0.87 μg/mL for wild shrimp and 0.69 μg/mL for farmed shrimp. These results suggest that, at the mycotoxins concentrations used in the study, AP from farmed
L. vannamei
was sensitive to the presence of both mycotoxins; however, AP is more sensitive to the combination of AFB
1
+ FB
1
suggesting a possible synergistic or potentiating inhibitory effect.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>27040818</pmid><doi>10.1007/s12550-016-0246-x</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0178-7888 |
| ispartof | Mycotoxin research, 2016-08, Vol.32 (3), p.117-125 |
| issn | 0178-7888 1867-1632 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1805768606 |
| source | MEDLINE; Springer Online Journals Complete |
| subjects | Aflatoxin B1 - metabolism Alkaline Phosphatase - antagonists & inhibitors Animals Biomedical and Life Sciences Chemistry/Food Science Enzyme Inhibitors - metabolism Fumonisins - metabolism Hepatopancreas - enzymology Inhibitory Concentration 50 Life Sciences Medical Microbiology Medicine/Public Health Microbiology Original Article Penaeidae - enzymology |
| title | An in vitro study of alkaline phosphatase sensitivity to mixture of aflatoxin B1 and fumonisin B1 in the hepatopancreas of coastal lagoon wild and farmed shrimp Litopenaeus vannamei |
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