Antibacterial properties and mechanism of graphene oxide-silver nanocomposites as bactericidal agents for water disinfection
Providing clean and affordable drinking water without harmful disinfection byproducts generated by conventional chemical disinfectants gives rise to the need for technological innovation. Nanotechnology has great potential in purifying water and wastewater treatment. A graphene oxide-silver (GO-Ag)...
Gespeichert in:
| Veröffentlicht in: | Archives of biochemistry and biophysics 2016-08, Vol.604, p.167-176 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 176 |
|---|---|
| container_issue | |
| container_start_page | 167 |
| container_title | Archives of biochemistry and biophysics |
| container_volume | 604 |
| creator | Song, Biao Zhang, Chang Zeng, Guangming Gong, Jilai Chang, Yingna Jiang, Yan |
| description | Providing clean and affordable drinking water without harmful disinfection byproducts generated by conventional chemical disinfectants gives rise to the need for technological innovation. Nanotechnology has great potential in purifying water and wastewater treatment. A graphene oxide-silver (GO-Ag) nanocomposite with excellent antibacterial activity was prepared and characterized by transmission electron microscope and X-ray photoelectron spectroscopy. The tests were carried out using Escherichia coli and Staphylococcus aureus as model strains of Gram-negative and Gram-positive bacteria, respectively. The effect of bactericide dosage and pH on antibacterial activity of GO-Ag was examined. Morphological observation of bacterial cells by scanning electron microscope showed that GO-Ag was much more destructive to cell membrane of Escherichia coli than that of Staphylococcus aureus. Experiments were carried out using catalase, superoxide dismutase and sodium thioglycollate to investigate the formation of reactive oxygen species and free silver ions in the bactericidal process. The activity of intracellular antioxidant enzymes was measured to investigate the potential role of oxidative stress. According to the consequence, synergetic mechanism including destruction of cell membranes and oxidative stress accounted for the antibacterial activity of GO-Ag nanocomposites. All the results suggested that GO-Ag nanocomposites displayed a good potential for application in water disinfection.
[Display omitted]
•Graphene oxide-silver (GO-Ag) nanocomposites with excellent antibacterial activity in aqueous solutions were prepared.•The antibacterial activity relied on the concentration of GO-Ag.•GO-Ag nanocomposites were much more destructive to cell membrane of Escherichia coli than that of Staphylococcus aureus.•Silver ions dissolved form silver nanoparticles were the main substance to directly kill bacteria.•Synergetic mechanism accounted for the antibacterial activity of GO-Ag nanocomposites. |
| doi_str_mv | 10.1016/j.abb.2016.04.018 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1805766202</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0003986116301527</els_id><sourcerecordid>1805766202</sourcerecordid><originalsourceid>FETCH-LOGICAL-c419t-c39e738675dfd7f4852d95e06d1cf88d48a2a9d973960d610777f939b6caebe83</originalsourceid><addsrcrecordid>eNp9kE1v1DAQhi1ERbeFH8AF-cglYZxk_SFOVVWgUqVeytly7HHrVWIHO1tA6o_Hq104cprR6H0faR5C3jNoGTD-adeacWy7urYwtMDkK7JhoHgDvRxekw0A9I2SnJ2Ti1J2AIwNvHtDzjvBBHCADXm5imsYjV0xBzPRJacF8xqwUBMdndE-mRjKTJOnj9ksTxiRpl_BYVPC9IyZRhOTTfOSSlgPrUJPNBtcBZpHjGuhPmX609QzdaGE6NGuIcW35MybqeC707wk37_cPFx_a-7uv95eX901dmBqbWyvUPSSi63zTvhBbjuntgjcMeuldIM0nVFOiV5xcJyBEMKrXo3cGhxR9pfk45Fb3_uxx7LqORSL02Qipn3RTMJWcN5BV6PsGLU5lZLR6yWH2eTfmoE-SNc7XaXrg3QNg67Sa-fDCb8fZ3T_Gn8t18DnYwDrk88Bsy42YLToQq4mtEvhP_g_FzaUxg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1805766202</pqid></control><display><type>article</type><title>Antibacterial properties and mechanism of graphene oxide-silver nanocomposites as bactericidal agents for water disinfection</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Song, Biao ; Zhang, Chang ; Zeng, Guangming ; Gong, Jilai ; Chang, Yingna ; Jiang, Yan</creator><creatorcontrib>Song, Biao ; Zhang, Chang ; Zeng, Guangming ; Gong, Jilai ; Chang, Yingna ; Jiang, Yan</creatorcontrib><description>Providing clean and affordable drinking water without harmful disinfection byproducts generated by conventional chemical disinfectants gives rise to the need for technological innovation. Nanotechnology has great potential in purifying water and wastewater treatment. A graphene oxide-silver (GO-Ag) nanocomposite with excellent antibacterial activity was prepared and characterized by transmission electron microscope and X-ray photoelectron spectroscopy. The tests were carried out using Escherichia coli and Staphylococcus aureus as model strains of Gram-negative and Gram-positive bacteria, respectively. The effect of bactericide dosage and pH on antibacterial activity of GO-Ag was examined. Morphological observation of bacterial cells by scanning electron microscope showed that GO-Ag was much more destructive to cell membrane of Escherichia coli than that of Staphylococcus aureus. Experiments were carried out using catalase, superoxide dismutase and sodium thioglycollate to investigate the formation of reactive oxygen species and free silver ions in the bactericidal process. The activity of intracellular antioxidant enzymes was measured to investigate the potential role of oxidative stress. According to the consequence, synergetic mechanism including destruction of cell membranes and oxidative stress accounted for the antibacterial activity of GO-Ag nanocomposites. All the results suggested that GO-Ag nanocomposites displayed a good potential for application in water disinfection.
[Display omitted]
•Graphene oxide-silver (GO-Ag) nanocomposites with excellent antibacterial activity in aqueous solutions were prepared.•The antibacterial activity relied on the concentration of GO-Ag.•GO-Ag nanocomposites were much more destructive to cell membrane of Escherichia coli than that of Staphylococcus aureus.•Silver ions dissolved form silver nanoparticles were the main substance to directly kill bacteria.•Synergetic mechanism accounted for the antibacterial activity of GO-Ag nanocomposites.</description><identifier>ISSN: 0003-9861</identifier><identifier>EISSN: 1096-0384</identifier><identifier>DOI: 10.1016/j.abb.2016.04.018</identifier><identifier>PMID: 27170600</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Anti-Bacterial Agents - chemistry ; Antibacterial ; Catalase - metabolism ; Disinfection ; Escherichia coli - enzymology ; Graphene oxide ; Graphite - chemistry ; Hydrogen-Ion Concentration ; Ions ; Lipid Peroxidation ; Metal Nanoparticles - chemistry ; Nanocomposites - chemistry ; Oxidative Stress ; Oxides - chemistry ; Photoelectron Spectroscopy ; Silver - chemistry ; Silver nanoparticles ; Staphylococcus aureus - enzymology ; Superoxide Dismutase - metabolism ; Thioglycolates - metabolism ; Waste Water ; Water - chemistry ; Water disinfection ; Water Microbiology ; Water Purification - methods</subject><ispartof>Archives of biochemistry and biophysics, 2016-08, Vol.604, p.167-176</ispartof><rights>2016 Elsevier Inc.</rights><rights>Copyright © 2016 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c419t-c39e738675dfd7f4852d95e06d1cf88d48a2a9d973960d610777f939b6caebe83</citedby><cites>FETCH-LOGICAL-c419t-c39e738675dfd7f4852d95e06d1cf88d48a2a9d973960d610777f939b6caebe83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.abb.2016.04.018$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27170600$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Song, Biao</creatorcontrib><creatorcontrib>Zhang, Chang</creatorcontrib><creatorcontrib>Zeng, Guangming</creatorcontrib><creatorcontrib>Gong, Jilai</creatorcontrib><creatorcontrib>Chang, Yingna</creatorcontrib><creatorcontrib>Jiang, Yan</creatorcontrib><title>Antibacterial properties and mechanism of graphene oxide-silver nanocomposites as bactericidal agents for water disinfection</title><title>Archives of biochemistry and biophysics</title><addtitle>Arch Biochem Biophys</addtitle><description>Providing clean and affordable drinking water without harmful disinfection byproducts generated by conventional chemical disinfectants gives rise to the need for technological innovation. Nanotechnology has great potential in purifying water and wastewater treatment. A graphene oxide-silver (GO-Ag) nanocomposite with excellent antibacterial activity was prepared and characterized by transmission electron microscope and X-ray photoelectron spectroscopy. The tests were carried out using Escherichia coli and Staphylococcus aureus as model strains of Gram-negative and Gram-positive bacteria, respectively. The effect of bactericide dosage and pH on antibacterial activity of GO-Ag was examined. Morphological observation of bacterial cells by scanning electron microscope showed that GO-Ag was much more destructive to cell membrane of Escherichia coli than that of Staphylococcus aureus. Experiments were carried out using catalase, superoxide dismutase and sodium thioglycollate to investigate the formation of reactive oxygen species and free silver ions in the bactericidal process. The activity of intracellular antioxidant enzymes was measured to investigate the potential role of oxidative stress. According to the consequence, synergetic mechanism including destruction of cell membranes and oxidative stress accounted for the antibacterial activity of GO-Ag nanocomposites. All the results suggested that GO-Ag nanocomposites displayed a good potential for application in water disinfection.
[Display omitted]
•Graphene oxide-silver (GO-Ag) nanocomposites with excellent antibacterial activity in aqueous solutions were prepared.•The antibacterial activity relied on the concentration of GO-Ag.•GO-Ag nanocomposites were much more destructive to cell membrane of Escherichia coli than that of Staphylococcus aureus.•Silver ions dissolved form silver nanoparticles were the main substance to directly kill bacteria.•Synergetic mechanism accounted for the antibacterial activity of GO-Ag nanocomposites.</description><subject>Anti-Bacterial Agents - chemistry</subject><subject>Antibacterial</subject><subject>Catalase - metabolism</subject><subject>Disinfection</subject><subject>Escherichia coli - enzymology</subject><subject>Graphene oxide</subject><subject>Graphite - chemistry</subject><subject>Hydrogen-Ion Concentration</subject><subject>Ions</subject><subject>Lipid Peroxidation</subject><subject>Metal Nanoparticles - chemistry</subject><subject>Nanocomposites - chemistry</subject><subject>Oxidative Stress</subject><subject>Oxides - chemistry</subject><subject>Photoelectron Spectroscopy</subject><subject>Silver - chemistry</subject><subject>Silver nanoparticles</subject><subject>Staphylococcus aureus - enzymology</subject><subject>Superoxide Dismutase - metabolism</subject><subject>Thioglycolates - metabolism</subject><subject>Waste Water</subject><subject>Water - chemistry</subject><subject>Water disinfection</subject><subject>Water Microbiology</subject><subject>Water Purification - methods</subject><issn>0003-9861</issn><issn>1096-0384</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1v1DAQhi1ERbeFH8AF-cglYZxk_SFOVVWgUqVeytly7HHrVWIHO1tA6o_Hq104cprR6H0faR5C3jNoGTD-adeacWy7urYwtMDkK7JhoHgDvRxekw0A9I2SnJ2Ti1J2AIwNvHtDzjvBBHCADXm5imsYjV0xBzPRJacF8xqwUBMdndE-mRjKTJOnj9ksTxiRpl_BYVPC9IyZRhOTTfOSSlgPrUJPNBtcBZpHjGuhPmX609QzdaGE6NGuIcW35MybqeC707wk37_cPFx_a-7uv95eX901dmBqbWyvUPSSi63zTvhBbjuntgjcMeuldIM0nVFOiV5xcJyBEMKrXo3cGhxR9pfk45Fb3_uxx7LqORSL02Qipn3RTMJWcN5BV6PsGLU5lZLR6yWH2eTfmoE-SNc7XaXrg3QNg67Sa-fDCb8fZ3T_Gn8t18DnYwDrk88Bsy42YLToQq4mtEvhP_g_FzaUxg</recordid><startdate>20160815</startdate><enddate>20160815</enddate><creator>Song, Biao</creator><creator>Zhang, Chang</creator><creator>Zeng, Guangming</creator><creator>Gong, Jilai</creator><creator>Chang, Yingna</creator><creator>Jiang, Yan</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20160815</creationdate><title>Antibacterial properties and mechanism of graphene oxide-silver nanocomposites as bactericidal agents for water disinfection</title><author>Song, Biao ; Zhang, Chang ; Zeng, Guangming ; Gong, Jilai ; Chang, Yingna ; Jiang, Yan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c419t-c39e738675dfd7f4852d95e06d1cf88d48a2a9d973960d610777f939b6caebe83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Anti-Bacterial Agents - chemistry</topic><topic>Antibacterial</topic><topic>Catalase - metabolism</topic><topic>Disinfection</topic><topic>Escherichia coli - enzymology</topic><topic>Graphene oxide</topic><topic>Graphite - chemistry</topic><topic>Hydrogen-Ion Concentration</topic><topic>Ions</topic><topic>Lipid Peroxidation</topic><topic>Metal Nanoparticles - chemistry</topic><topic>Nanocomposites - chemistry</topic><topic>Oxidative Stress</topic><topic>Oxides - chemistry</topic><topic>Photoelectron Spectroscopy</topic><topic>Silver - chemistry</topic><topic>Silver nanoparticles</topic><topic>Staphylococcus aureus - enzymology</topic><topic>Superoxide Dismutase - metabolism</topic><topic>Thioglycolates - metabolism</topic><topic>Waste Water</topic><topic>Water - chemistry</topic><topic>Water disinfection</topic><topic>Water Microbiology</topic><topic>Water Purification - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Song, Biao</creatorcontrib><creatorcontrib>Zhang, Chang</creatorcontrib><creatorcontrib>Zeng, Guangming</creatorcontrib><creatorcontrib>Gong, Jilai</creatorcontrib><creatorcontrib>Chang, Yingna</creatorcontrib><creatorcontrib>Jiang, Yan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of biochemistry and biophysics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Song, Biao</au><au>Zhang, Chang</au><au>Zeng, Guangming</au><au>Gong, Jilai</au><au>Chang, Yingna</au><au>Jiang, Yan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antibacterial properties and mechanism of graphene oxide-silver nanocomposites as bactericidal agents for water disinfection</atitle><jtitle>Archives of biochemistry and biophysics</jtitle><addtitle>Arch Biochem Biophys</addtitle><date>2016-08-15</date><risdate>2016</risdate><volume>604</volume><spage>167</spage><epage>176</epage><pages>167-176</pages><issn>0003-9861</issn><eissn>1096-0384</eissn><abstract>Providing clean and affordable drinking water without harmful disinfection byproducts generated by conventional chemical disinfectants gives rise to the need for technological innovation. Nanotechnology has great potential in purifying water and wastewater treatment. A graphene oxide-silver (GO-Ag) nanocomposite with excellent antibacterial activity was prepared and characterized by transmission electron microscope and X-ray photoelectron spectroscopy. The tests were carried out using Escherichia coli and Staphylococcus aureus as model strains of Gram-negative and Gram-positive bacteria, respectively. The effect of bactericide dosage and pH on antibacterial activity of GO-Ag was examined. Morphological observation of bacterial cells by scanning electron microscope showed that GO-Ag was much more destructive to cell membrane of Escherichia coli than that of Staphylococcus aureus. Experiments were carried out using catalase, superoxide dismutase and sodium thioglycollate to investigate the formation of reactive oxygen species and free silver ions in the bactericidal process. The activity of intracellular antioxidant enzymes was measured to investigate the potential role of oxidative stress. According to the consequence, synergetic mechanism including destruction of cell membranes and oxidative stress accounted for the antibacterial activity of GO-Ag nanocomposites. All the results suggested that GO-Ag nanocomposites displayed a good potential for application in water disinfection.
[Display omitted]
•Graphene oxide-silver (GO-Ag) nanocomposites with excellent antibacterial activity in aqueous solutions were prepared.•The antibacterial activity relied on the concentration of GO-Ag.•GO-Ag nanocomposites were much more destructive to cell membrane of Escherichia coli than that of Staphylococcus aureus.•Silver ions dissolved form silver nanoparticles were the main substance to directly kill bacteria.•Synergetic mechanism accounted for the antibacterial activity of GO-Ag nanocomposites.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>27170600</pmid><doi>10.1016/j.abb.2016.04.018</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-9861 |
| ispartof | Archives of biochemistry and biophysics, 2016-08, Vol.604, p.167-176 |
| issn | 0003-9861 1096-0384 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1805766202 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Anti-Bacterial Agents - chemistry Antibacterial Catalase - metabolism Disinfection Escherichia coli - enzymology Graphene oxide Graphite - chemistry Hydrogen-Ion Concentration Ions Lipid Peroxidation Metal Nanoparticles - chemistry Nanocomposites - chemistry Oxidative Stress Oxides - chemistry Photoelectron Spectroscopy Silver - chemistry Silver nanoparticles Staphylococcus aureus - enzymology Superoxide Dismutase - metabolism Thioglycolates - metabolism Waste Water Water - chemistry Water disinfection Water Microbiology Water Purification - methods |
| title | Antibacterial properties and mechanism of graphene oxide-silver nanocomposites as bactericidal agents for water disinfection |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-20T12%3A09%3A15IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Antibacterial%20properties%20and%20mechanism%20of%20graphene%20oxide-silver%20nanocomposites%20as%20bactericidal%20agents%20for%20water%20disinfection&rft.jtitle=Archives%20of%20biochemistry%20and%20biophysics&rft.au=Song,%20Biao&rft.date=2016-08-15&rft.volume=604&rft.spage=167&rft.epage=176&rft.pages=167-176&rft.issn=0003-9861&rft.eissn=1096-0384&rft_id=info:doi/10.1016/j.abb.2016.04.018&rft_dat=%3Cproquest_cross%3E1805766202%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1805766202&rft_id=info:pmid/27170600&rft_els_id=S0003986116301527&rfr_iscdi=true |