Extra virgin olive oil rich in polyphenols modulates VEGF-induced angiogenic responses by preventing NADPH oxidase activity and expression
Previous studies have shown the antiinflammatory, antioxidant and antiangiogenic properties by pure olive oil polyphenols; however, the effects of olive oil phenolic fraction on the inflammatory angiogenesis are unknown. In this study, we investigated the effects of the phenolic fraction (olive oil...
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| Veröffentlicht in: | The Journal of nutritional biochemistry 2016-02, Vol.28, p.19-29 |
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| creator | Calabriso, Nadia Massaro, Marika Scoditti, Egeria D’Amore, Simona Gnoni, Antonio Pellegrino, Mariangela Storelli, Carlo De Caterina, Raffaele Palasciano, Giuseppe Carluccio, Maria Annunziata |
| description | Previous studies have shown the antiinflammatory, antioxidant and antiangiogenic properties by pure olive oil polyphenols; however, the effects of olive oil phenolic fraction on the inflammatory angiogenesis are unknown. In this study, we investigated the effects of the phenolic fraction (olive oil polyphenolic extract, OOPE) from extra virgin olive oil and related circulating metabolites on the VEGF-induced angiogenic responses and NADPH oxidase activity and expression in human cultured endothelial cells. We found that OOPE (1–10 μg/ml), at concentrations achievable nutritionally, significantly reduced, in a concentration-dependent manner, the VEGF-induced cell migration, invasiveness and tube-like structure formation through the inhibition of MMP-2 and MMP-9. OOPE significantly (P |
| doi_str_mv | 10.1016/j.jnutbio.2015.09.026 |
| format | Article |
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[Display omitted]</description><identifier>ISSN: 0955-2863</identifier><identifier>EISSN: 1873-4847</identifier><identifier>DOI: 10.1016/j.jnutbio.2015.09.026</identifier><identifier>PMID: 26878779</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Endothelial cells ; Endothelium, Vascular - cytology ; Endothelium, Vascular - drug effects ; Endothelium, Vascular - enzymology ; Human Umbilical Vein Endothelial Cells ; Humans ; Matrix metalloproteinases ; NADPH Oxidases - antagonists & inhibitors ; Neovascularization, Pathologic - prevention & control ; Nox2 ; Nox4 ; Olea ; Olive Oil - chemistry ; Oxidative stress ; p47phox ; Polyphenols - pharmacology ; Vascular Endothelial Growth Factor A - physiology</subject><ispartof>The Journal of nutritional biochemistry, 2016-02, Vol.28, p.19-29</ispartof><rights>2015 Elsevier Inc.</rights><rights>Copyright © 2015 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c464t-51ecc1ef8152cd9ad43244a1855ba31f1100b386994b027b0eaf1e0879188f363</citedby><cites>FETCH-LOGICAL-c464t-51ecc1ef8152cd9ad43244a1855ba31f1100b386994b027b0eaf1e0879188f363</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0955286315002776$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26878779$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Calabriso, Nadia</creatorcontrib><creatorcontrib>Massaro, Marika</creatorcontrib><creatorcontrib>Scoditti, Egeria</creatorcontrib><creatorcontrib>D’Amore, Simona</creatorcontrib><creatorcontrib>Gnoni, Antonio</creatorcontrib><creatorcontrib>Pellegrino, Mariangela</creatorcontrib><creatorcontrib>Storelli, Carlo</creatorcontrib><creatorcontrib>De Caterina, Raffaele</creatorcontrib><creatorcontrib>Palasciano, Giuseppe</creatorcontrib><creatorcontrib>Carluccio, Maria Annunziata</creatorcontrib><title>Extra virgin olive oil rich in polyphenols modulates VEGF-induced angiogenic responses by preventing NADPH oxidase activity and expression</title><title>The Journal of nutritional biochemistry</title><addtitle>J Nutr Biochem</addtitle><description>Previous studies have shown the antiinflammatory, antioxidant and antiangiogenic properties by pure olive oil polyphenols; however, the effects of olive oil phenolic fraction on the inflammatory angiogenesis are unknown. In this study, we investigated the effects of the phenolic fraction (olive oil polyphenolic extract, OOPE) from extra virgin olive oil and related circulating metabolites on the VEGF-induced angiogenic responses and NADPH oxidase activity and expression in human cultured endothelial cells. We found that OOPE (1–10 μg/ml), at concentrations achievable nutritionally, significantly reduced, in a concentration-dependent manner, the VEGF-induced cell migration, invasiveness and tube-like structure formation through the inhibition of MMP-2 and MMP-9. OOPE significantly (P<0.05) reduced VEGF-induced intracellular reactive oxygen species by modulating NADPH oxidase activity, p47phox membrane translocation and the expression of Nox2 and Nox4. Moreover, the treatment of endothelial cells with serum obtained 4 h after acute intake of extra virgin olive oil, with high polyphenol content, decreased VEGF-induced NADPH oxidase activity and Nox4 expression, as well as, MMP-9 expression, as compared with fasting control serum. Overall, native polyphenols and serum metabolites of extra virgin olive oil rich in polyphenols are able to lower the VEGF-induced angiogenic responses by preventing endothelial NADPH oxidase activity and decreasing the expression of selective NADPH oxidase subunits. Our results provide an alternative mechanism by which the consumption of olive oil rich in polyphenols may account for a reduction of oxidative stress inflammatory-related sequelae associated with chronic degenerative diseases.
[Display omitted]</description><subject>Endothelial cells</subject><subject>Endothelium, Vascular - cytology</subject><subject>Endothelium, Vascular - drug effects</subject><subject>Endothelium, Vascular - enzymology</subject><subject>Human Umbilical Vein Endothelial Cells</subject><subject>Humans</subject><subject>Matrix metalloproteinases</subject><subject>NADPH Oxidases - antagonists & inhibitors</subject><subject>Neovascularization, Pathologic - prevention & control</subject><subject>Nox2</subject><subject>Nox4</subject><subject>Olea</subject><subject>Olive Oil - chemistry</subject><subject>Oxidative stress</subject><subject>p47phox</subject><subject>Polyphenols - pharmacology</subject><subject>Vascular Endothelial Growth Factor A - physiology</subject><issn>0955-2863</issn><issn>1873-4847</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAQhi0EokvhEUA-cskyTuLEOaGq3bZIFXAArpbjTLazytrBTqLdV-CpcbUL155GGn3__NJ8jL0XsBYgqk-79c7NU0t-nYOQa2jWkFcv2EqoushKVdYv2QoaKbNcVcUFexPjDgDyUlav2UVeqVrVdbNifzaHKRi-UNiS436gBbmngQeyjzxtRj8cx0d0foh877t5MBNG_mtzd5uR62aLHTduS36LjiwPGEfvYiLaIx8DLugmclv-9erm-z33B-pMRG7sRAtNx5TsOB4SFyN595a96s0Q8d15XrKft5sf1_fZw7e7L9dXD5ktq3LKpEBrBfZKyNx2jenKIi9LI5SUrSlELwRAW6iqacoW8roFNL1AUHUjlOqLqrhkH093x-B_zxgnvadocRiMQz9HLRRICRIKeB6tK9mIOldFQuUJtcHHGLDXY6C9CUctQD8Z0zt9NqafjGlodDKWch_OFXO7x-5_6p-iBHw-AZh-shAGHS2hS5-ngHbSnadnKv4CsDyr8g</recordid><startdate>201602</startdate><enddate>201602</enddate><creator>Calabriso, Nadia</creator><creator>Massaro, Marika</creator><creator>Scoditti, Egeria</creator><creator>D’Amore, Simona</creator><creator>Gnoni, Antonio</creator><creator>Pellegrino, Mariangela</creator><creator>Storelli, Carlo</creator><creator>De Caterina, Raffaele</creator><creator>Palasciano, Giuseppe</creator><creator>Carluccio, Maria Annunziata</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>201602</creationdate><title>Extra virgin olive oil rich in polyphenols modulates VEGF-induced angiogenic responses by preventing NADPH oxidase activity and expression</title><author>Calabriso, Nadia ; Massaro, Marika ; Scoditti, Egeria ; D’Amore, Simona ; Gnoni, Antonio ; Pellegrino, Mariangela ; Storelli, Carlo ; De Caterina, Raffaele ; Palasciano, Giuseppe ; Carluccio, Maria Annunziata</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c464t-51ecc1ef8152cd9ad43244a1855ba31f1100b386994b027b0eaf1e0879188f363</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Endothelial cells</topic><topic>Endothelium, Vascular - cytology</topic><topic>Endothelium, Vascular - drug effects</topic><topic>Endothelium, Vascular - enzymology</topic><topic>Human Umbilical Vein Endothelial Cells</topic><topic>Humans</topic><topic>Matrix metalloproteinases</topic><topic>NADPH Oxidases - antagonists & inhibitors</topic><topic>Neovascularization, Pathologic - prevention & control</topic><topic>Nox2</topic><topic>Nox4</topic><topic>Olea</topic><topic>Olive Oil - chemistry</topic><topic>Oxidative stress</topic><topic>p47phox</topic><topic>Polyphenols - pharmacology</topic><topic>Vascular Endothelial Growth Factor A - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Calabriso, Nadia</creatorcontrib><creatorcontrib>Massaro, Marika</creatorcontrib><creatorcontrib>Scoditti, Egeria</creatorcontrib><creatorcontrib>D’Amore, Simona</creatorcontrib><creatorcontrib>Gnoni, Antonio</creatorcontrib><creatorcontrib>Pellegrino, Mariangela</creatorcontrib><creatorcontrib>Storelli, Carlo</creatorcontrib><creatorcontrib>De Caterina, Raffaele</creatorcontrib><creatorcontrib>Palasciano, Giuseppe</creatorcontrib><creatorcontrib>Carluccio, Maria Annunziata</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>The Journal of nutritional biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Calabriso, Nadia</au><au>Massaro, Marika</au><au>Scoditti, Egeria</au><au>D’Amore, Simona</au><au>Gnoni, Antonio</au><au>Pellegrino, Mariangela</au><au>Storelli, Carlo</au><au>De Caterina, Raffaele</au><au>Palasciano, Giuseppe</au><au>Carluccio, Maria Annunziata</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Extra virgin olive oil rich in polyphenols modulates VEGF-induced angiogenic responses by preventing NADPH oxidase activity and expression</atitle><jtitle>The Journal of nutritional biochemistry</jtitle><addtitle>J Nutr Biochem</addtitle><date>2016-02</date><risdate>2016</risdate><volume>28</volume><spage>19</spage><epage>29</epage><pages>19-29</pages><issn>0955-2863</issn><eissn>1873-4847</eissn><abstract>Previous studies have shown the antiinflammatory, antioxidant and antiangiogenic properties by pure olive oil polyphenols; however, the effects of olive oil phenolic fraction on the inflammatory angiogenesis are unknown. In this study, we investigated the effects of the phenolic fraction (olive oil polyphenolic extract, OOPE) from extra virgin olive oil and related circulating metabolites on the VEGF-induced angiogenic responses and NADPH oxidase activity and expression in human cultured endothelial cells. We found that OOPE (1–10 μg/ml), at concentrations achievable nutritionally, significantly reduced, in a concentration-dependent manner, the VEGF-induced cell migration, invasiveness and tube-like structure formation through the inhibition of MMP-2 and MMP-9. OOPE significantly (P<0.05) reduced VEGF-induced intracellular reactive oxygen species by modulating NADPH oxidase activity, p47phox membrane translocation and the expression of Nox2 and Nox4. Moreover, the treatment of endothelial cells with serum obtained 4 h after acute intake of extra virgin olive oil, with high polyphenol content, decreased VEGF-induced NADPH oxidase activity and Nox4 expression, as well as, MMP-9 expression, as compared with fasting control serum. Overall, native polyphenols and serum metabolites of extra virgin olive oil rich in polyphenols are able to lower the VEGF-induced angiogenic responses by preventing endothelial NADPH oxidase activity and decreasing the expression of selective NADPH oxidase subunits. Our results provide an alternative mechanism by which the consumption of olive oil rich in polyphenols may account for a reduction of oxidative stress inflammatory-related sequelae associated with chronic degenerative diseases.
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| subjects | Endothelial cells Endothelium, Vascular - cytology Endothelium, Vascular - drug effects Endothelium, Vascular - enzymology Human Umbilical Vein Endothelial Cells Humans Matrix metalloproteinases NADPH Oxidases - antagonists & inhibitors Neovascularization, Pathologic - prevention & control Nox2 Nox4 Olea Olive Oil - chemistry Oxidative stress p47phox Polyphenols - pharmacology Vascular Endothelial Growth Factor A - physiology |
| title | Extra virgin olive oil rich in polyphenols modulates VEGF-induced angiogenic responses by preventing NADPH oxidase activity and expression |
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