Anti-melanogenic effects of Aster spathulifolius extract in UVB-exposed C57BL/6J mice and B16F10 melanoma cells through the regulation of MAPK/ERK and AKT/GSK3β signalling
Objectives Pharmacological studies of Aster spathulifolius Maxim(AS) have demonstrated its anti‐allergy, anti‐viral and anti‐obesity effects, however, its anti‐melanogenic effects is still unclear. In this study, the effects of AS extract (ASE) on the inhibition of melanin synthesis were investigate...
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| Veröffentlicht in: | Journal of pharmacy and pharmacology 2016-04, Vol.68 (4), p.503-513 |
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| creator | Hwang, Ga Yeon Choung, Se-Young |
| description | Objectives
Pharmacological studies of Aster spathulifolius Maxim(AS) have demonstrated its anti‐allergy, anti‐viral and anti‐obesity effects, however, its anti‐melanogenic effects is still unclear. In this study, the effects of AS extract (ASE) on the inhibition of melanin synthesis were investigated in vitro and in vivo.
Methods
To perform this study, the contents of melanin and tyrosinase activity were analysed in B16F10 melanoma cells. Western blotting was carried out to determine the underlyling mechanism. Additionally, we investigated the effect of this extract on hyperpigmentation in C57bL/6J mice induced by 3, 6 and 9 weeks of UVB irradiation.
Key findings
AS extract led to reduced melanin synthesis through the regulation of MITF and its downstream signals. Furthermore, ASE increased the phosphorylation of MAPK/ERK and Akt/GSK3β signalling pathway components. In vivo study, hypopigmentation effects were also observed. The melanocyte activity and the distribution of melanin granules were decreased in UVB‐irradiated mice treated with ASE.
Conclusions
These results suggest that the ASE may be promising as an active anti‐melanogenic component, and further investigations should be performed regarding its potential as a whitening agent in the field of cosmetics. |
| doi_str_mv | 10.1111/jphp.12524 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1805498690</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1805498690</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4004-af9325ea62aeed9cc17aa0dc0e9b6bb6c55d2cbe6470f8cbad99ad5d23aa61373</originalsourceid><addsrcrecordid>eNp9kctu00AUhi0EoqGw4QHQLBGSm7l4ZuKlE5qUJECgLSxH4_GxM2V8wWOL9J1Y8SA8E07ddslsjjT6_u8c6Q-C1wSfkeFNb5p9c0Yop9GTYEJxRENJ-OxpMMGY0pBxyU6CF97fYIylEOJ5cEJFHDPM5ST4nVSdDUtwuqoLqKxBkOdgOo_qHCW-gxb5Rnf73tm8drb3CA5dq02HbIWuv81DODS1hwwtuJxvp2KNSmsA6SpDcyKWBKPRXWpkwDmPun1b98V-mIBaKHqnO1tXx20fk91mev51cxdONlfT1eWG_f2DvC0q7ZytipfBs1w7D6_u52lwvTy_WlyE28-rD4tkG5oI4yjUecwoBy2oBshiY4jUGmcGQ5yKNBWG84yaFEQkcT4zqc7iWGfDH9NaECbZafB29DZt_bMH36nS-uP5uoK694rMMI_imYjxgL4bUdPW3reQq6a1pW5vFcHq2I46tqPu2hngN_fePi0he0Qf6hgAMgK_rIPb_6jUenexe5CGY8YObR0eM7r9oYRkkqvvn1ZKXJL58v06Vl_YP8CIquc</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1805498690</pqid></control><display><type>article</type><title>Anti-melanogenic effects of Aster spathulifolius extract in UVB-exposed C57BL/6J mice and B16F10 melanoma cells through the regulation of MAPK/ERK and AKT/GSK3β signalling</title><source>MEDLINE</source><source>Wiley Journals</source><source>Oxford University Press Journals All Titles (1996-Current)</source><creator>Hwang, Ga Yeon ; Choung, Se-Young</creator><creatorcontrib>Hwang, Ga Yeon ; Choung, Se-Young</creatorcontrib><description>Objectives
Pharmacological studies of Aster spathulifolius Maxim(AS) have demonstrated its anti‐allergy, anti‐viral and anti‐obesity effects, however, its anti‐melanogenic effects is still unclear. In this study, the effects of AS extract (ASE) on the inhibition of melanin synthesis were investigated in vitro and in vivo.
Methods
To perform this study, the contents of melanin and tyrosinase activity were analysed in B16F10 melanoma cells. Western blotting was carried out to determine the underlyling mechanism. Additionally, we investigated the effect of this extract on hyperpigmentation in C57bL/6J mice induced by 3, 6 and 9 weeks of UVB irradiation.
Key findings
AS extract led to reduced melanin synthesis through the regulation of MITF and its downstream signals. Furthermore, ASE increased the phosphorylation of MAPK/ERK and Akt/GSK3β signalling pathway components. In vivo study, hypopigmentation effects were also observed. The melanocyte activity and the distribution of melanin granules were decreased in UVB‐irradiated mice treated with ASE.
Conclusions
These results suggest that the ASE may be promising as an active anti‐melanogenic component, and further investigations should be performed regarding its potential as a whitening agent in the field of cosmetics.</description><identifier>ISSN: 0022-3573</identifier><identifier>EISSN: 2042-7158</identifier><identifier>DOI: 10.1111/jphp.12524</identifier><identifier>PMID: 26993057</identifier><language>eng</language><publisher>England: Blackwell Publishing Ltd</publisher><subject><![CDATA[Akt/GSK3β ; Animals ; Aster Plant - chemistry ; Aster spathulifolius maxim ; B16F10 ; C57bl/6J ; Cell Line, Tumor ; Chromatography, High Pressure Liquid ; Disease Models, Animal ; Dose-Response Relationship, Drug ; Extracellular Signal-Regulated MAP Kinases - antagonists & inhibitors ; Extracellular Signal-Regulated MAP Kinases - metabolism ; Glycogen Synthase Kinase 3 beta - antagonists & inhibitors ; Glycogen Synthase Kinase 3 beta - metabolism ; Hyperpigmentation - enzymology ; Hyperpigmentation - prevention & control ; MAPK/ERK ; melanin synthesis ; melanin synthesis, Aster spathulifolius maxim ; Melanins - metabolism ; Melanocytes - drug effects ; Melanocytes - enzymology ; Melanoma, Experimental - drug therapy ; Melanoma, Experimental - enzymology ; Melanoma, Experimental - pathology ; Mice, Inbred C57BL ; Microphthalmia-Associated Transcription Factor - metabolism ; Monophenol Monooxygenase - metabolism ; Phosphorylation ; Phytotherapy ; Plant Extracts - isolation & purification ; Plant Extracts - pharmacology ; Plants, Medicinal ; Protein Kinase Inhibitors - pharmacology ; Proto-Oncogene Proteins c-akt - antagonists & inhibitors ; Proto-Oncogene Proteins c-akt - metabolism ; Signal Transduction - drug effects ; Skin - drug effects ; Skin - enzymology ; Skin Lightening Preparations - isolation & purification ; Skin Lightening Preparations - pharmacology ; Skin Neoplasms - drug therapy ; Skin Neoplasms - enzymology ; Skin Neoplasms - pathology ; Skin Pigmentation - drug effects ; Spectrometry, Mass, Electrospray Ionization ; Time Factors ; Ultraviolet Rays]]></subject><ispartof>Journal of pharmacy and pharmacology, 2016-04, Vol.68 (4), p.503-513</ispartof><rights>2016 Royal Pharmaceutical Society</rights><rights>2016 Royal Pharmaceutical Society, Journal of Pharmacy and Pharmacology.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4004-af9325ea62aeed9cc17aa0dc0e9b6bb6c55d2cbe6470f8cbad99ad5d23aa61373</citedby><cites>FETCH-LOGICAL-c4004-af9325ea62aeed9cc17aa0dc0e9b6bb6c55d2cbe6470f8cbad99ad5d23aa61373</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjphp.12524$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjphp.12524$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26993057$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hwang, Ga Yeon</creatorcontrib><creatorcontrib>Choung, Se-Young</creatorcontrib><title>Anti-melanogenic effects of Aster spathulifolius extract in UVB-exposed C57BL/6J mice and B16F10 melanoma cells through the regulation of MAPK/ERK and AKT/GSK3β signalling</title><title>Journal of pharmacy and pharmacology</title><addtitle>J Pharm Pharmacol</addtitle><description>Objectives
Pharmacological studies of Aster spathulifolius Maxim(AS) have demonstrated its anti‐allergy, anti‐viral and anti‐obesity effects, however, its anti‐melanogenic effects is still unclear. In this study, the effects of AS extract (ASE) on the inhibition of melanin synthesis were investigated in vitro and in vivo.
Methods
To perform this study, the contents of melanin and tyrosinase activity were analysed in B16F10 melanoma cells. Western blotting was carried out to determine the underlyling mechanism. Additionally, we investigated the effect of this extract on hyperpigmentation in C57bL/6J mice induced by 3, 6 and 9 weeks of UVB irradiation.
Key findings
AS extract led to reduced melanin synthesis through the regulation of MITF and its downstream signals. Furthermore, ASE increased the phosphorylation of MAPK/ERK and Akt/GSK3β signalling pathway components. In vivo study, hypopigmentation effects were also observed. The melanocyte activity and the distribution of melanin granules were decreased in UVB‐irradiated mice treated with ASE.
Conclusions
These results suggest that the ASE may be promising as an active anti‐melanogenic component, and further investigations should be performed regarding its potential as a whitening agent in the field of cosmetics.</description><subject>Akt/GSK3β</subject><subject>Animals</subject><subject>Aster Plant - chemistry</subject><subject>Aster spathulifolius maxim</subject><subject>B16F10</subject><subject>C57bl/6J</subject><subject>Cell Line, Tumor</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Disease Models, Animal</subject><subject>Dose-Response Relationship, Drug</subject><subject>Extracellular Signal-Regulated MAP Kinases - antagonists & inhibitors</subject><subject>Extracellular Signal-Regulated MAP Kinases - metabolism</subject><subject>Glycogen Synthase Kinase 3 beta - antagonists & inhibitors</subject><subject>Glycogen Synthase Kinase 3 beta - metabolism</subject><subject>Hyperpigmentation - enzymology</subject><subject>Hyperpigmentation - prevention & control</subject><subject>MAPK/ERK</subject><subject>melanin synthesis</subject><subject>melanin synthesis, Aster spathulifolius maxim</subject><subject>Melanins - metabolism</subject><subject>Melanocytes - drug effects</subject><subject>Melanocytes - enzymology</subject><subject>Melanoma, Experimental - drug therapy</subject><subject>Melanoma, Experimental - enzymology</subject><subject>Melanoma, Experimental - pathology</subject><subject>Mice, Inbred C57BL</subject><subject>Microphthalmia-Associated Transcription Factor - metabolism</subject><subject>Monophenol Monooxygenase - metabolism</subject><subject>Phosphorylation</subject><subject>Phytotherapy</subject><subject>Plant Extracts - isolation & purification</subject><subject>Plant Extracts - pharmacology</subject><subject>Plants, Medicinal</subject><subject>Protein Kinase Inhibitors - pharmacology</subject><subject>Proto-Oncogene Proteins c-akt - antagonists & inhibitors</subject><subject>Proto-Oncogene Proteins c-akt - metabolism</subject><subject>Signal Transduction - drug effects</subject><subject>Skin - drug effects</subject><subject>Skin - enzymology</subject><subject>Skin Lightening Preparations - isolation & purification</subject><subject>Skin Lightening Preparations - pharmacology</subject><subject>Skin Neoplasms - drug therapy</subject><subject>Skin Neoplasms - enzymology</subject><subject>Skin Neoplasms - pathology</subject><subject>Skin Pigmentation - drug effects</subject><subject>Spectrometry, Mass, Electrospray Ionization</subject><subject>Time Factors</subject><subject>Ultraviolet Rays</subject><issn>0022-3573</issn><issn>2042-7158</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kctu00AUhi0EoqGw4QHQLBGSm7l4ZuKlE5qUJECgLSxH4_GxM2V8wWOL9J1Y8SA8E07ddslsjjT6_u8c6Q-C1wSfkeFNb5p9c0Yop9GTYEJxRENJ-OxpMMGY0pBxyU6CF97fYIylEOJ5cEJFHDPM5ST4nVSdDUtwuqoLqKxBkOdgOo_qHCW-gxb5Rnf73tm8drb3CA5dq02HbIWuv81DODS1hwwtuJxvp2KNSmsA6SpDcyKWBKPRXWpkwDmPun1b98V-mIBaKHqnO1tXx20fk91mev51cxdONlfT1eWG_f2DvC0q7ZytipfBs1w7D6_u52lwvTy_WlyE28-rD4tkG5oI4yjUecwoBy2oBshiY4jUGmcGQ5yKNBWG84yaFEQkcT4zqc7iWGfDH9NaECbZafB29DZt_bMH36nS-uP5uoK694rMMI_imYjxgL4bUdPW3reQq6a1pW5vFcHq2I46tqPu2hngN_fePi0he0Qf6hgAMgK_rIPb_6jUenexe5CGY8YObR0eM7r9oYRkkqvvn1ZKXJL58v06Vl_YP8CIquc</recordid><startdate>201604</startdate><enddate>201604</enddate><creator>Hwang, Ga Yeon</creator><creator>Choung, Se-Young</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>201604</creationdate><title>Anti-melanogenic effects of Aster spathulifolius extract in UVB-exposed C57BL/6J mice and B16F10 melanoma cells through the regulation of MAPK/ERK and AKT/GSK3β signalling</title><author>Hwang, Ga Yeon ; Choung, Se-Young</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4004-af9325ea62aeed9cc17aa0dc0e9b6bb6c55d2cbe6470f8cbad99ad5d23aa61373</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Akt/GSK3β</topic><topic>Animals</topic><topic>Aster Plant - chemistry</topic><topic>Aster spathulifolius maxim</topic><topic>B16F10</topic><topic>C57bl/6J</topic><topic>Cell Line, Tumor</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Disease Models, Animal</topic><topic>Dose-Response Relationship, Drug</topic><topic>Extracellular Signal-Regulated MAP Kinases - antagonists & inhibitors</topic><topic>Extracellular Signal-Regulated MAP Kinases - metabolism</topic><topic>Glycogen Synthase Kinase 3 beta - antagonists & inhibitors</topic><topic>Glycogen Synthase Kinase 3 beta - metabolism</topic><topic>Hyperpigmentation - enzymology</topic><topic>Hyperpigmentation - prevention & control</topic><topic>MAPK/ERK</topic><topic>melanin synthesis</topic><topic>melanin synthesis, Aster spathulifolius maxim</topic><topic>Melanins - metabolism</topic><topic>Melanocytes - drug effects</topic><topic>Melanocytes - enzymology</topic><topic>Melanoma, Experimental - drug therapy</topic><topic>Melanoma, Experimental - enzymology</topic><topic>Melanoma, Experimental - pathology</topic><topic>Mice, Inbred C57BL</topic><topic>Microphthalmia-Associated Transcription Factor - metabolism</topic><topic>Monophenol Monooxygenase - metabolism</topic><topic>Phosphorylation</topic><topic>Phytotherapy</topic><topic>Plant Extracts - isolation & purification</topic><topic>Plant Extracts - pharmacology</topic><topic>Plants, Medicinal</topic><topic>Protein Kinase Inhibitors - pharmacology</topic><topic>Proto-Oncogene Proteins c-akt - antagonists & inhibitors</topic><topic>Proto-Oncogene Proteins c-akt - metabolism</topic><topic>Signal Transduction - drug effects</topic><topic>Skin - drug effects</topic><topic>Skin - enzymology</topic><topic>Skin Lightening Preparations - isolation & purification</topic><topic>Skin Lightening Preparations - pharmacology</topic><topic>Skin Neoplasms - drug therapy</topic><topic>Skin Neoplasms - enzymology</topic><topic>Skin Neoplasms - pathology</topic><topic>Skin Pigmentation - drug effects</topic><topic>Spectrometry, Mass, Electrospray Ionization</topic><topic>Time Factors</topic><topic>Ultraviolet Rays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hwang, Ga Yeon</creatorcontrib><creatorcontrib>Choung, Se-Young</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Journal of pharmacy and pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hwang, Ga Yeon</au><au>Choung, Se-Young</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Anti-melanogenic effects of Aster spathulifolius extract in UVB-exposed C57BL/6J mice and B16F10 melanoma cells through the regulation of MAPK/ERK and AKT/GSK3β signalling</atitle><jtitle>Journal of pharmacy and pharmacology</jtitle><addtitle>J Pharm Pharmacol</addtitle><date>2016-04</date><risdate>2016</risdate><volume>68</volume><issue>4</issue><spage>503</spage><epage>513</epage><pages>503-513</pages><issn>0022-3573</issn><eissn>2042-7158</eissn><abstract>Objectives
Pharmacological studies of Aster spathulifolius Maxim(AS) have demonstrated its anti‐allergy, anti‐viral and anti‐obesity effects, however, its anti‐melanogenic effects is still unclear. In this study, the effects of AS extract (ASE) on the inhibition of melanin synthesis were investigated in vitro and in vivo.
Methods
To perform this study, the contents of melanin and tyrosinase activity were analysed in B16F10 melanoma cells. Western blotting was carried out to determine the underlyling mechanism. Additionally, we investigated the effect of this extract on hyperpigmentation in C57bL/6J mice induced by 3, 6 and 9 weeks of UVB irradiation.
Key findings
AS extract led to reduced melanin synthesis through the regulation of MITF and its downstream signals. Furthermore, ASE increased the phosphorylation of MAPK/ERK and Akt/GSK3β signalling pathway components. In vivo study, hypopigmentation effects were also observed. The melanocyte activity and the distribution of melanin granules were decreased in UVB‐irradiated mice treated with ASE.
Conclusions
These results suggest that the ASE may be promising as an active anti‐melanogenic component, and further investigations should be performed regarding its potential as a whitening agent in the field of cosmetics.</abstract><cop>England</cop><pub>Blackwell Publishing Ltd</pub><pmid>26993057</pmid><doi>10.1111/jphp.12524</doi><tpages>11</tpages></addata></record> |
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| ispartof | Journal of pharmacy and pharmacology, 2016-04, Vol.68 (4), p.503-513 |
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| source | MEDLINE; Wiley Journals; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Akt/GSK3β Animals Aster Plant - chemistry Aster spathulifolius maxim B16F10 C57bl/6J Cell Line, Tumor Chromatography, High Pressure Liquid Disease Models, Animal Dose-Response Relationship, Drug Extracellular Signal-Regulated MAP Kinases - antagonists & inhibitors Extracellular Signal-Regulated MAP Kinases - metabolism Glycogen Synthase Kinase 3 beta - antagonists & inhibitors Glycogen Synthase Kinase 3 beta - metabolism Hyperpigmentation - enzymology Hyperpigmentation - prevention & control MAPK/ERK melanin synthesis melanin synthesis, Aster spathulifolius maxim Melanins - metabolism Melanocytes - drug effects Melanocytes - enzymology Melanoma, Experimental - drug therapy Melanoma, Experimental - enzymology Melanoma, Experimental - pathology Mice, Inbred C57BL Microphthalmia-Associated Transcription Factor - metabolism Monophenol Monooxygenase - metabolism Phosphorylation Phytotherapy Plant Extracts - isolation & purification Plant Extracts - pharmacology Plants, Medicinal Protein Kinase Inhibitors - pharmacology Proto-Oncogene Proteins c-akt - antagonists & inhibitors Proto-Oncogene Proteins c-akt - metabolism Signal Transduction - drug effects Skin - drug effects Skin - enzymology Skin Lightening Preparations - isolation & purification Skin Lightening Preparations - pharmacology Skin Neoplasms - drug therapy Skin Neoplasms - enzymology Skin Neoplasms - pathology Skin Pigmentation - drug effects Spectrometry, Mass, Electrospray Ionization Time Factors Ultraviolet Rays |
| title | Anti-melanogenic effects of Aster spathulifolius extract in UVB-exposed C57BL/6J mice and B16F10 melanoma cells through the regulation of MAPK/ERK and AKT/GSK3β signalling |
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