Acid-inducible proton influx currents in the plasma membrane of murine osteoclast-like cells

Acidification of the resorption pits, which is essential for dissolving bone, is produced by secretion of protons through vacuolar H + -ATPases in the plasma membrane of bone-resorbing cells, osteoclasts. Consequently, osteoclasts face highly acidic extracellular environments, where the pH gradient across the plasma membrane could generate a force driving protons into the cells. Proton influx mechanisms during the acid exposure are largely unknown, however. In this study, we investigated extracellular-acid-inducible proton influx currents in osteoclast-like cells derived from a macrophage cell line (RAW264). Decreasing extracellular pH to